• Journal of Aquaculture
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• v.16 no.1
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• pp.51-58
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• 2003

The first Indian transgenic fish was generated in 1991 using borrowed constructs from foreign sources. To construct transformation vectors for the indigenous fishes, growth hormone genes of rohu (r-CH), Labeo rohita and catfish, Heteropneustes fossilis were isolated, cloned and sequenced; their fidelity was confirmed in prokaryotic and eukaryotic systems. A vector was constructed with grass carp b-actin promoter driving the expression of r-GH. Rohu eggs are large. fragile and swell 2~3 times. when fertilized. Hence they were amenable only for electroporated sperm-mediated gene transfer. Accordingly, the sperm electroporation technique was standardized to ensure 25% hatchling survival and 37% Presumptive transgenics without suffering any deformity. Southern analysis confirmed genomic integration in 15% of the tested individuals (Ti) belonging to family lines 2 and 3: another 25% of the Juveniles (Te) were also proved transgenic but with the transgene persisting extrachromosomally for longer than 1 to 2 years. perhaps due to the presence of replicon in the vector. Transgenics belonging to different family lines grew 6~8 times faster than the respective controls. Difference in growth trends of Ti and Te within a family line was not significant. In the Ti family 3 remarkable growth acceleration was sustained for a period longer than 36 weeks but in those of family 2, it gradually decreased. All transgenic fishes including the rohu converted the food at a significantly higher efficiency. Barring the transgenic mudloach, all the other transgenic fishes consumed food at significantly reduced rate.

• Ocean and Polar Research
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• v.40 no.1
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• pp.49-58
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• 2018

This study aimed to analyze the causality of hairtail prices among import and domestic distribution channels using a Vector Error Correction Model(VECM). The results are as follows. First, since the ADF unit-root test suggests that each of the price variables, apart from retail price, has a unit root, the price variables should be 1st-differenced to secure the stability of the prices. Next, through the Johansen co-integration test, it was discovered that there are long-term relationships among the price variables. On the basis of the co-integration test, VECM analysis shows that the producer price has a long-run balance with the import and wholesale prices. In particular, when the prices deviate from the balance, the producer price dynamically adjusts to return to the long-term relationship among prices. It also indicates that the producer price has an impact on the import, wholesale, and retail prices in the short-term, and the import price has an influence on the producer and wholesale prices. In addition, the impulse response analysis demonstrates that the impulse of import and producer prices has a lasting impact on each of the prices.

• Korean Journal of Animal Reproduction
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• v.25 no.4
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• pp.399-407
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• 2001

The microinjection of retroviral vectors into the perivitelline spaces of MII-stage oocytes increased production of transgenic bovine embryos. However, oocytes have various sizes of perivitelline space, and there is the tendency that the oocyte membranes are damageable by micropipettes during the injection of retroviral vectors into perivitelline spaces or oocytes. Thus, it was not always possible to stably inject retroviral vector into perivitelline spaces of oocytes. Here we used sucrose to minimize the damage of the oocyte membrane. When the oocytes were suspended in 0.5% sucrose, poor quality oocytes showed rough cytoplasmic membranes, while good quality oocytes maintained smooth membranes. However, when the tatters were subjected to in vitro fertilization, no significant differences were observed in cleavage rates (82% of control Vs. 84% of sucrose treated oocytes). The Same trends were obtained from the oocytes fertilized after microinjection of LN$\beta$-EGFP and LNC-hGH genes into the perivitelline spares. The rates of cleavage and blastocyst from microinjection of LN$\beta$-EGFP genes were 81 and 25%, and from microinjection of LNC-hGM genes were 53 and 30%, respectively. The result indicated that microinjected oocytes could develop to the blastocyst stages after in vitro fertilization with no significant difference from control group. Moreover, the integration of hGH-gene (by PCR analysis) was detected in 52% of infected cleaved embryos and the expression of EGFP-gene (under a fluoresrence microscope) was also observed in 34% of infected blastocyst. These results indicated that 0.5% sucrose treatment could be an efficient method not only to select good quality embryos but also to inject retroviral vectors into perivitelline spares without any harm and hence improving developmental rates.

• Geophysics and Geophysical Exploration
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• v.27 no.1
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• pp.51-56
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• 2024

In this paper, the vector gravity and gravity gradient tensor of an elliptical disk are derived. The vector gravity of an elliptical disk is defined by differentiating the gravitational potential due to the elliptical disk expressed by a double integral with respect to each axial direction. The vector gravity defined by the double integral is then transformed into a line integral of a closed curve along the elliptical disk boundary using the complex Green's theorem. Finally, vector gravity due to the elliptical disk is derived by 1D parametric numerical integration along the elliptical disk boundary. The xz, yz, zz components of the gravity gradient tensor due to the elliptical disk are obtained by differentiating the vector gravity with respect to vertical direction. The xx, yy, xy components are derived by differentiating the horizontal components of the vector gravity in the form of a double integral with respect to horizontal directions and then using the complex Green's theorem.

• Journal of the Institute of Electronics Engineers of Korea CI
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• v.49 no.2
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• pp.96-102
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• 2012

An advanced form of the Partitioned Feature-based Classifier with Expertise Table(PFC-ET) is proposed in this paper. As is the case with the PFC-ET, the proposed classifier model, called Classifier Integration Model(CIM), does not use the entire feature vectors extracted from the original data in a concatenated form to classify each datum, but rather uses groups of features related to each feature vector separately. The proposed CIM utilizes a proportion of selected cluster members instead of the expertise table in PFC-ET to minimize the error in confusion table. The proposed CIM is applied to the classification problem on two data sets, Caltech data set and collected terrain data sets. When compared with PFC model and PFC-ET model. the proposed CIM shows improvements in terms of classification accuracy and post processing efforts.

• Structural Engineering and Mechanics
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• v.57 no.2
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• pp.327-355
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• 2016

A new analytical derivation of the elastodynamic point load solutions for an isotropic multi-layered half-space is presented by means of the precise integration method (PIM) and the approach of dual vector. The time-harmonic external load is prescribed either on the external boundary or in the interior of the solid medium. Starting with the axisymmetric governing motion equations in a cylindrical coordinate system, a second order ordinary differential matrix equation can be gained by making use of the Hankel integral transform. Employing the technique of dual vector, the second order ordinary differential matrix equation can be simplified into a first-order one. The approach of PIM is implemented to obtain the solutions of the ordinary differential matrix equation in the Hankel integral transform domain. The PIM is a highly accurate algorithm to solve sets of first-order ordinary differential equations and any desired accuracy of the dynamic point load solutions can be achieved. The numerical simulation is based on algebraic matrix operation. As a result, the computational effort is reduced to a great extent and the computation is unconditionally stable. Selected numerical trials are given to validate the accuracy and applicability of the proposed approach. More examples are discussed to portray the dependence of the load-displacement response on the isotropic parameters of the multi-layered media, the depth of external load and the frequency of excitation.

• Journal of Positioning, Navigation, and Timing
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• v.9 no.3
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• pp.207-214
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• 2020

In this paper, we implement linear-quadratic-Gaussian based vector tracking loop (LQG-VTL) instead of conventional extended Kalman filter based vector tracking loop (EKF-VTL). The LQG-VTL can improve the performance compared to the EKF-VTL by generating optimal control input at a specific performance index. Performance analysis is conducted through two factors, frequency thermal noise and frequency dynamic stress error, which determine total frequency tracking error. We derive the thermal noise and the dynamic stress error formula in the LQG-VTL. From frequency tracking error analysis, we can determine control gain matrix in the LQG controller and show that the frequency tracking error of the LQG-VTL is lower than that of the EKF-VTL in all C/N0 ranges. The simulation results show that the LQG-VTL improves performance by 30% in Doppler tracking, so the LQG-VTL can extend pre-integration time longer and track weaker signals than the EKF-VTL. Therefore, the LQG-VTL algorithm is more robust than the EKF-VTL in weak signal environments.

• Journal of Plant Biotechnology
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• v.46 no.4
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• pp.323-330
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• 2019

The high expression level of industrial and metabolically important proteins in plants can be achieved by plastid transformation. The CaIA vector, a Capsicum-specific vector harboring aadA (spectinomycin resistance), is a selectable marker controlled by the PsbA promoter, and the terminator is flanked by the trnA and trnI regions of the inverted repeat (IR) region of the plastid. The CaIA vector can introduce foreign genes into the IR region of the plastid genome. The biolistic method was used for chloroplast transformation in Scoparia dulcis with leaf explants followed by antibiotic selection on regeneration medium. Transplastomes were successfully screened, and the transformation efficiency of 3 transgenic lines from 25 bombarded leaf explants was determined. Transplastomic lines were evaluated by PCR and Southern blotting for the confirmation of aadA insertion and its integration into the chloroplast genome. Seeds collected from transplastomes were analyzed on spectinomycin medium with wild types to determine genetic stability. The increased chloroplast transformation efficiency (3 transplastomic lines from 25 bombarded explants) would be useful for expressing therapeutically and industrially important genes in Scoparia dulcis L.

• Journal of Microbiology and Biotechnology
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• v.16 no.9
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• pp.1362-1368
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• 2006

A new constitutive episomal expression vector, pGAPZ-E, was constructed and used for initial screening of eukaryotic target gene expression in Pichia pastoris. Two reporter genes such as beta-galactosidase gene and GFPuv gene were overexpressed in P. pastoris. The expression level of the episomal pGAPZ-E strain was higher than that of the integrated form when the beta-galactosidase gene was used as the reporter gene in P. pastoris X33. The avoiding of both the integration procedure and an induction step simplified the overall screening process for eukaryotic target gene expression in P. pastoris. Nine human protein targets from the Core 50, family of Northeast Structural Genomics Consortium (http://www.nesg.org), which were intractable when expressed in E. coli, were subjected to rapid screening for soluble expression in P. pastoris. HR547, HR919, and HR1697 human proteins, which had previously been found to express poorly or to be insoluble in E. coli, expressed in soluble form in P. pastoris. Therefore, the new episomal GAP promoter vector provides a convenient and alternative system for high-throughput screening of eukaryotic protein expression in P. pastoris.

• Journal of Computational Design and Engineering
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• v.2 no.2
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• pp.88-95
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• 2015

Recently, fiber composite materials have been attracting attention from industry because of their remarkable material characteristics, including light weight and high stiffness. However, the costs of products composed of fiber materials remain high because of the lack of effective manufacturing and designing technologies. To improve the relevant design technology, this paper proposes a novel simulation method for deforming fiber materials. Specifically, given a 3D model with constant thickness and known fiber orientation, the proposed method simulates the deformation of a model made of thick fiber-material. The method separates a 3D sheet model into two surfaces and then flattens these surfaces into two dimensional planes by a parameterization method with involves cross vector fields. The cross vector fields are generated by propagating the given fiber orientations specified at several important points on the 3D model. Integration of the cross vector fields gives parameterization with low-stretch and low-distortion.