• East Asian mathematical journal
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• 제15권2호
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• pp.255-265
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• 1999

• Journal of Microbiology and Biotechnology
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• 제25권11호
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• pp.1863-1870
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• 2015

Fibrinolytic enzyme genes (aprE2, aprE176, and aprE179) were introduced into the Bacillus subtilis 168 chromosome without any antibiotic resistance gene. An integration vector, pDG1662, was used to deliver the genes into the amyE site of B. subtilis 168. Integrants, SJ3-5nc, SJ176nc, and SJ179nc, were obtained after two successive homologous recombinations. The integration of each fibrinolytic gene into the middle of the amyE site was confirmed by phenotypes (Amy-, Spec^S) and colony PCR results for these strains. The fibrinolytic activities of the integrants were higher than that of B. subtilis 168 by at least 3.2-fold when grown in LB broth. Cheonggukjang was prepared by inoculating each of B. subtilis 168, SJ3-5nc, SJ176nc, and SJ179nc, and the fibrinolytic activity of cheonggukjang was 4.6 ± 0.7, 10.8 ± 0.9, 7.0 ± 0.6, and 8.0 ± 0.2 (U/g of cheonggukjang), respectively at 72 h. These results showed that construction of B. subtilis strains with enhanced fibrinolytic activities is possible by integration of a strong fibrinolytic gene via a marker-free manner.

• 한국우주과학회:학술대회논문집(한국우주과학회보)
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• 한국우주과학회 2004년도 한국우주과학회보 제13권2호
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• pp.296-299
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• 2004

KARI precision attitude determination system has been developed for high accurate geo-coding of KOMPSAT-2 image. Sensor data from two star trackers and a IRU are used as measurement and dynamic data. Sensor data from star tracker are composed of QUEST and unit vector filter. Filter algorithms consists of extended Kalman filter, unscented Kalman filter, and least square batch filter. The type of sensor data and filter algorithm can be chosen by user options. Estimated parameters are Euler angle from 12000 frame to optical bench frame, gyro drift rate bias, gyro scale factor, misalignment angle of star tracker coordinate frame with respect to optical bench frame, and misalignment angle of gyro coordinate frame with respect to optical bench frame. In particular, ground control point data can be applied for estimating misalignment angle of star tracker coordinate frame. Through the simulation, KPADS is able to satisfy the KOMPSAT-2 mission requirement in which geo-location accuracy of image is 80 m (CE90) without ground control point.

• 한국미생물·생명공학회지
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• 제47권4호
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• pp.667-672
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• 2019

본 연구는 출아효모 Saccharomyces cerevisiae을 이용해 이종 유전자(heterologous gene)를 효모염색체내에 도입하여 안정적으로 발현하기 위한 시스템의 비교에 대해서 연구하였다. 반복적으로 사용할 수 있는 Cre/loxP system의 이용을 위해 C. glabrata 유래 유전자를 선택마커로 사용하였고, universal pRS-CMT vector를 이용한 4종의 유전자(XYLP, XYLB, GRE3 및 XYL2 유전자)를 모델 유전자로 cloning하였다. 구축된 pRS-XylP, pRS-XylB, pRS-Gre3 및 pRS-Xyl2 plasmid를 이용한 4번의 sequential integration을 통해 효모염색체내에 도입된 4종의 유전자를 순차적으로 발현시킬 수 있었다. 또한 4종의 유전자 발현 cassette를 동시에 가지는 pRS-PBG2 plasmid에 의한 one-step integration을 통해서, 도입될 유전자들의 순서를 정할 수 있었으며 각 유전자들의 동시발현을 안정적으로 유지할 수 있었다. 결론적으로 본 연구에서 사용한 4종의 유전자들의 염색체내 동시 integration 및 발현을 위해서는 one-step integration이 효과적임을 확인하였으며, 적절한 유전자 도입방법을 통해 산업적으로 유용한 생물시스템의 손쉬운 육종이 가능하리라 기대한다.

• Journal of Microbiology and Biotechnology
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• 제30권9호
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• pp.1273-1281
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• 2020

Due to the broad host suitability of viral vectors and their high gene delivery capacity, many researchers are focusing on viral vector-mediated gene therapy. Among the retroviruses, foamy viruses have been considered potential gene therapy vectors because of their non-pathogenicity. To date, the prototype foamy virus is the only retrovirus that has a high-resolution structure of intasomes, nucleoprotein complexes formed by integrase, and viral DNA. The integration of viral DNA into the host chromosome is an essential step for viral vector development. This process is mediated by virally encoded integrase, which catalyzes unique chemical reactions. Additionally, recent studies on foamy virus integrase elucidated the catalytic functions of its three distinct domains and their effect on viral pathogenicity. This review focuses on recent advancements in biochemical, structural, and functional studies of foamy virus integrase for gene therapy vector research.

• Structural Engineering and Mechanics
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• 제67권6호
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• pp.603-616
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• 2018

In this study, the acceleration vector in each time step is assumed to be a mth order time polynomial. By using the initial conditions, satisfying the equation of motion at both ends of the time step and minimizing the square of the residual vector, the m+3 unknown coefficients are determined. The order of accuracy for this approach is m+1, and it has a very low dispersion error. Moreover, the period error of the new technique is almost zero, and it is considerably smaller than the members of the Newmark method. The proposed scheme has an appropriate domain of stability, which is greater than that of the central difference and linear acceleration techniques. The numerical tests highlight the improved performance of the new algorithm over the fourth-order Runge-Kutta, central difference, linear and average acceleration methods.

• Mycobiology
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• 제43권1호
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• pp.1-8
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• 2015

Mushroom transformation requires a series of experimental steps, including generation of host strains with a desirable selective marker, design of vector DNA, removal of host cell wall, introduction of foreign DNA across the cell membrane, and integration into host genomic DNA or maintenance of an autonomous vector DNA inside the host cell. This review introduces limitations and obstacles related to transformation technologies along with possible solutions. Current methods for cell wall removal and cell membrane permeabilization are summarized together with details of two popular technologies, Agrobacterium tumefaciens-mediated transformation and restriction enzyme-mediated integration.

• 소성∙가공
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• 제12권7호
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• pp.615-622
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• 2003

In the automobile industry, the design of optimal blank shape becomes a significant part of the stamping. It provides many evident advantages, sush as enhancement of formability, reduction of material cost and product development period. However, the nesting process, required for the optimal usage of materials in the blanking becomes more complicated as the blank shape becomes complicated, like most optimal blank shape. In this study, stamping process optimization system for the optimal usage of material has been developed through the integration of optimal blank design and optimal nesting. For optimal blank design, a radius vector method, the modified version of the initial nodal velocity method, the past work of the present author, have been proposed. Both the optimal blank design and optimal nesting programs have been developed under the GUI environment for the convenience of engineers. The efficiency of the optimization system has been verified with some chosen problems.

• 한국콘텐츠학회:학술대회논문집
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• 한국콘텐츠학회 2007년도 추계 종합학술대회 논문집
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• pp.530-534
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• 2007

유동 가시화란 가시화 기술의 한 영역으로, 벡터 데이터를 2차원 또는 3차원의 형태로 시각적으로 표출하는 것을 말한다. 즉, 일반적으로 벡터 데이터는 (x, y, z)의 형식으로 이루어져 있는 수열의 집합인데, 이를 사람이 그 특징을 쉽게 인지할 수 있도록 그림 또는 애니메이션으로 표시하는 것을 말한다. 유동 가시화는 가시화 기법, 대상 데이터의 차원, 대상 유동의 종류 등 여러 가지 기준으로 분류가 가능하다. 가시화 기법은 크게 직접 기법, 인티그레이션(integration) 기법, 파생 데이터 기반 기법 등으로 나눌 수 있고, 데이터의 차원은 2차원, 2.5차원, 3차원 등으로 구분할 수 있으며, 유동의 종류는 일정한(steady) 유동과 불규칙한(unsteady) 유동으로 나눌 수 있다. 이러한 유동 가시화는 그 종류가 매우 많고 다양한데, 본 논문에서는 대표적인 인티그레이션 기법인 LIC 기법에 초점을 맞추고 각 기법들을 데이터의 차원으로 분류하고 각 기법의 장단점을 논하고자 한다.

• 제어로봇시스템학회:학술대회논문집
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• 제어로봇시스템학회 2003년도 ICCAS
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• pp.2641-2647
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• 2003

Conventional DLLs estimate the delay times of satellite signals individually and feed back these measurements to the VCO independently. But VDLL estimates delay times and user position directly and then estimate the feedback term for VCO using the estimated position changes. In this process, input measurements are treated as vectors and these vectors are used for navigation. First advantage of VDLL is that noise is reduced in all of the tracking channels making them less likely to enter the nonlinear region and fall below threshold. Second is that VDLL can operate successfully when the conventional independent parallel DLL approach fails completely. It means that VDLL receiver can get enough total signal power to track successfully to obtain accurate position estimates under the same conditions where the signal strength from each individual satellite is so low or week that none of the individual scalar DLL can remain in lock when operating independently. To operate VDLL successfully, it needs to know the initial user dynamics and position and prevents total system from the divergence. The suggested integration method is to use the inertial navigation system to provide initial dynamics for VDLL and to maintain total system stable. We designed the GPS/INS integrated navigation system. This new type of integrated system contained the vector pseudorange format generation block, VDLL signal processing block, position estimation block and the conversion block from position change to delay time feedback term aided by INS.