• Journal of the Korea Academia-Industrial cooperation Society
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• v.13 no.10
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• pp.4462-4470
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• 2012

This study aims to examine the effects of process-based integrated Mathematics Science Activities on children's science process skills and scientific attitudes and arrange efficient teaching and learning environment in the field. The subjects of research were 89 three-year and five-year old children attending kindergarten attached to W university in I city, Jeollabuk-do and process-based integrated Mathematics Science activities were conducted with themes. For data analysis, paired t-test was conducted with SPSS for Windows program and as a result of performing process-based integrated Mathematics Science activities, it was found that there were significant differences in sub-factors of science process skills and scientific attitude by age and sex. These results suggested that process-based integrated activities had positive influences on three-year and five-yeard old children.

• The Journal of the Korea Contents Association
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• v.18 no.5
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• pp.123-130
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• 2018

The present study aims to investigate how Korean EFL learners' views of form-focused instruction, integrated and isolated FFI (form-focused instruction), are related to their beliefs about grammar and attention and how different these relationships are between high and low proficiency levels and between males and females in Korean college contexts. The findings indicated the participants' strong preference for integrated FFI, which was significantly correlated with two factors, attention in English class and English proficiency. On the other hand, the isolated FFI was strongly correlated with their beliefs about grammar learning, that is, independent learning of grammar and importance of learning grammar rules. In conclusion, the integrated FFI was associated with students' proficiency and attention, while the isolated FFI was related to their views of grammar learning. In conclusion, it is suggested that we need to use integrated FFI in Korean EFL contexts considering students' levels of proficiency and attention.

• Journal of Microbiology and Biotechnology
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• v.18 no.1
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• pp.35-42
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• 2008

Vibrio vulnificus produces siderophores, low-molecular-weight iron-chelating compounds, to obtain iron under conditions of iron deprivation. To identify genes associated with the biosynthesis of siderophore in V. vulnificus MO6-24/O, we screened clones with mini-Tn5 random insertions for those showing decreased production of siderophore. Among 6,000 clones screened, nine such clones were selected. These clones contain the transposon inserted in VV2_0830 (GenBank accession number) that is a homolog of a nonribosomal peptide synthase (NRPS). There is an another NRPS module, VV2_0831, 49-bp upstream to VV2_0830. We named these two genes vvs (Vibrio vulnificus siderophore synthase) A and B, respectively. Mutation of either vvsA or vvsB showed a decreased production of siderophore. The expression of an NRPS-lux fusion was negatively modulated by the presence of iron, and the regulation was dependent on Fur (ferric uptake regulator). However, the expression of the NRPS genes was still not fully derepressed in the iron-rich condition, even in furnull mutant cells, suggesting that some other unknown factors are involved in the regulation of the genes. We also demonstrated that the NRPS genes are important for virulence of the pathogen in a mice model.

• Journal of Microbiology and Biotechnology
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• v.17 no.2
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• pp.234-243
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• 2007

Vibrio vulnificus is an opportunistic pathogen that causes septicemia in humans. To identify the genes associated with its pathogenicity, in vivo expression technology (IVET) was used to select genes specifically expressed in a host, yet not significantly in vitro. Random lacZ-fusions in the genome of V vulnificus strain MO6-24/O were constructed using an IVET vector, pSG3, which is a suicide vector containing promoterless-aph and -lacZ as reporter genes. A total of ${\sim}18,000$ resulting library clones were then intraperitoneally injected into BALB/c mice using a colony forming unit (CFU) of $1.6{\times}10^6$. Two hours after infection, kanamycin was administered at $200{mu}g$ per gram of mouse weight. After two selection cycles, 11 genes were eventually isolated, which were expressed only in the host. Among these genes, VV20781 and VV21007 exhibiting a homology to a hemagglutinin gene and tolC, respectively, were selected based on having the highest frequency. When compared to wild-type cells, mutants with lesions in these genes showed no difference in the rate of growth rate, yet a significant decrease in cytotoxicity and the capability to form a biofilm.

• Journal of Korea Entertainment Industry Association
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• v.15 no.2
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• pp.191-202
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• 2021

The purpose of this research was to investigate the effects of the communication ability, communication type and satisfaction of nursing service on the re-use intention of nursing and care integration services of the hospital. The research subjects were 291 patients who were hospitalized for more than 3 days in the integrated nursing and care service center. Data were analyzed using SPSS 21.0. As a result of the study, the variables that significantly affect the reuse of integrated nursing services in hospitals were satisfaction with nursing services and integrated services. The explanatory power of these variables was 63.4%. Therefore, to increase the intention to re-use integrated nursing and care services of the hospitals, efforts are needed to increase the satisfaction of nursing services. And research on other variables that affect the guarantee is highly recommended.

• Biomolecules & Therapeutics
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• v.30 no.5
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• pp.455-464
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• 2022

Efonidipine, a calcium channel blocker, is widely used for the treatment of hypertension and cardiovascular diseases. In our preliminary study using structure-based virtual screening, efonidipine was identified as a potential inhibitor of c-Jun N-terminal kinase 3 (JNK3). Although its antihypertensive effect is widely known, the role of efonidipine in the central nervous system has remained elusive. The present study investigated the effects of efonidipine on the inflammation and cell migration induced by lipopolysaccharide (LPS) using murine BV2 and human HMC3 microglial cell lines and elucidated signaling molecules mediating its effects. We found that the phosphorylations of JNK and its downstream molecule c-Jun in LPS-treated BV2 cells were declined by efonidipine, confirming the finding from virtual screening. In addition, efonidipine inhibited the LPS-induced production of pro-inflammatory factors, including interleukin-1β (IL-1β) and nitric oxide. Similarly, the IL-1β production in LPS-treated HMC3 cells was also inhibited by efonidipine. Efonidipine markedly impeded cell migration stimulated by LPS in both cells. Furthermore, it inhibited the phosphorylation of inhibitor kappa B, thereby suppressing nuclear translocation of nuclear factor-κB (NF-κB) in LPS-treated BV2 cells. Taken together, efonidipine exerts anti-inflammatory and anti-migratory effects in LPS-treated microglial cells through inhibition of the JNK/NF-κB pathway. These findings imply that efonidipine may be a potential candidate for drug repositioning, with beneficial impacts on brain disorders associated with neuroinflammation.

• Journal of Korea Society of Industrial Information Systems
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• v.15 no.2
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• pp.115-126
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• 2010

Modern society can be described as ubiquitous computing over the concept of information. Information technology(IT) has been developing in a way that relative technologies are integrated to each other. Especially in ubiquitous environment, medical information industry shows significant interest in the U-healthcare service area. This paper will first look into U-healthcare service environment and component of Integrated Medical Information Systems(IMIS). Secondly, it examines the basic technological factors for integrated medical information systems, which is datawarehouse, network, communication standards and technology related U-healthcare service. Finally it proposes how to implement and operate new integrated medical information system for ubiquitous health care service. The system will do point of care(POC) for customers by real time and diagnose them using their various and personal medical data. The information will be communicated back to the customers, which will improve their satisfaction.

• The Korean Journal of Physiology and Pharmacology
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• v.28 no.3
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• pp.229-237
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• 2024

Cough is a common symptom of several respiratory diseases. However, frequent coughing from acute to chronic often causes great pain to patients. It may turn into cough variant asthma, which seriously affects people's quality of life. For cough treatment, it is dominated by over-the-counter antitussive drugs, such as asmeton, but most currently available antitussive drugs have serious side effects. Thus, there is a great need for the development of new drugs with potent cough suppressant. BALB/c mice were used to construct mice model with cough to investigate the pharmacological effects of pectolinarigenin (PEC). Hematoxylin-eosin and Masson staining were used to assess lung injury and airway remodeling, and ELISA was used to assess the level of inflammatory factor release. In addition, inflammatory cell counts were measured to assess airway inflammation. Airway hyperresponsiveness assay was used to assess respiratory resistance in mice. Finally, we used Western blotting to explore the potential mechanisms of PEC. We found that PEC could alleviate lung tissue injury and reduce the release of inflammatory factors, inhibit of cough frequency and airway wall collagen deposition in mice model with cough. Meanwhile, PEC inhibited the Ras/ERK/c-Fos pathway to exhibit antitussive effect. Therefore, PEC may be a potential drug for cough suppression.

• Journal of the Korean Society for Aviation and Aeronautics
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• v.26 no.2
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• pp.31-38
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• 2018

The pilot aptitude test so far has been conducted with a dichotomous concept of cognitive factor and non-cognitive factor, so it was not easy to explain concrete factors about pilot aptitude. Therefore, an integrated approach is needed to construct pilot aptitude test including all factors that can predict pilot aptitude. In previous study, we developed questionnaire items for the Pilot Aptitude Personality Test(PAPT) which can predict the flight training achievement through personality and personal characteristics. And those items were categorized as personality, cognition, and motivation factors. The flight training grades were correlated with all the test factors of the items, and the cognitive factors were high. As a result of the regression analysis, the total score of the three factors together accounted for 16% of the flight training grades. The results of the ANOVA showed that the cognition factors and motivation factors had significant effects on the completion of the flight training. The factors of PAPT predicted not only the completion of the flight training but also the flight performance. This is because the existing paper-pencil pilot aptitude test can only discriminate the flight training completion status. This is also a result of the fact that the introductory flight training consists of various factors including not only basic cognition but also personality and motivation.

• Korean Journal of Ecology and Environment
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• v.53 no.1
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• pp.46-54
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• 2020

Artificial and natural changes such as weir construction and climate change often cause abnormal blooming of organism. Therefore, variations in species community of organisms have been actively investigated to identify influential environmental changes on the fresh water ecosystem. In this study, we investigated Chironomidae community and environmental factors at 5 representative weirs (Ipo, IP; Sejong, SJ; Juksan, JS; Gangjeong-goryung, GG; and Dalsung weir) in 4 Korean major rivers to figure out relationship between Chironomidae community and environmental factors. Environmental factors indicating organic matter (total organic carbon, TOC and Chlorophyll-a, Chl-a) showed lower concentration in IP and SJ compared with other sites(JS, GG and DS). 3 sub-family 18 genus 25 species of Chironomidae community were found in this study. Among them, Chironominae was dominant in JS (Tanytarsus sp.1), GG (Polypedilum scalaenum) and DS (Polypedilum scalaenum), while different sub-family were dominant in IP (Orthcladinae, Tokunagayusurika akamushi) and SJ (Tanypodinae, Tanypus punctipennis). Moreover, based on the dominant species of Chironomidae community and environmental factors, the cluster analysis classified our study sites into 3 groups. These results imply that the diet resource is the most important factor for dominance of Chironomidae in Korean rivers. We also suggest that further study on the identification of diet resources for each Chironomidae specie is required for better understating of distribution in species community of Chironomidae at various ecosystems.