• Proceedings of the Korean Society for Applied Microbiology Conference
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• 2004.06a
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• pp.60-61
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• 2004

Metabolic engineering is now a well established discipline, used extensively to determine and execute rational strategies of strain development to improve the performance of micro-organisms employed in industrial fermentations. The basic principle of this approach is that performance of the microbial catalyst should be adequately characterised metabolically so as to clearlyidentify the metabolic network constraints, thereby identifying the most probable targets for genetic engineering and the extent to which improvements can be realistically achieved. In order to harness correctly this potential, it is clear that the physiological analysis of each strain studied needs to be undertaken under conditions as close as possible to the physico-chemical environment in which the strain evolves within the full-scale process. Furthermore, this analysis needs to be undertaken throughoutthe entire fermentation so as to take into account the changing environment in an essentially dynamic situation in which metabolic stress is accentuated by the microbial activity itself, leading to increasingly important stress response at a metabolic level. All too often these industrial fermentation constraints are overlooked, leading to identification of targets whose validity within the industrial context is at best limited. Thus the conceptual error is linked to experimental design rather than inadequate methodology. New tools are becoming available which open up new possibilities in metabolic engineering and the characterisation of complex metabolic networks. Traditionally metabolic analysis was targeted towards pre-identified genes and their corresponding enzymatic activities within pre-selected metabolic pathways. Those pathways not included at the onset were intrinsically removed from the network giving a fundamentally localised vision of pathway functionality. New tools from genome research extend this reductive approach so as to include the global characteristics of a given biological model which can now be seen as an integrated functional unit rather than a specific sub-group of biochemical reactions, thereby facilitating the resolution of complexnetworks whose exact composition cannot be estimated at the onset. This global overview of whole cell physiology enables new targets to be identified which would classically not have been suspected previously. Of course, as with all powerful analytical tools, post-genomic technology must be used carefully so as to avoid expensive errors. This is not always the case and the data obtained need to be examined carefully to avoid embarking on the study of artefacts due to poor understanding of cell biology. These basic developments and the underlying concepts will be illustrated with examples from the author's laboratory concerning the industrial production of commodity chemicals using a number of industrially important bacteria. The different levels of possibleinvestigation and the extent to which the data can be extrapolated will be highlighted together with the extent to which realistic yield targets can be attained. Genetic engineering strategies and the performance of the resulting strains will be examined within the context of the prevailing experimental conditions encountered in the industrial fermentor. Examples used will include the production of amino acids, vitamins and polysaccharides. In each case metabolic constraints can be identified and the extent to which performance can be enhanced predicted

• Journal of Korea Water Resources Association
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• v.40 no.8
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• pp.601-615
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• 2007

A selective withdrawal method has been widely used to control the quality of water released from a stratified reservoir and to improve downstream ecosystem habitats. Recently, several existing reservoir withdrawal facilities have been modified to accommodate multi-level water intake capabilities in order to adapt the impact of long-term discharge of high turbidity flow. The purpose of this study was to assess the effect of selective withdrawal method on the control of downstream turbidity and its impact on water quality in Daecheong Reservoir. A laterally integrated two-dimensional hydrodynamic and eutrophication model, which was calibrated and validated in the previous studies, was applied to simulate the temporal variations of outflow turbidity with various hypothetical selective withdrawal scenarios. In addition, their impacts on the algal growth as well as water quality constituents were analyzed in three different spatial domains of the reservoir The results showed that the costly selective withdrawal method would provide very limited benefits for downstream turbidity control during two years of consecutive simulations for 2004-2005. In particular, an excessive withdrawal from the epilimnion zone for supplying upper layer clean water resulted in movement of turbidity plume that contained high phosphorus concentrations upward photic zone, and in turn increased algal growth in the lacustrine zone.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.21 no.5
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• pp.93-102
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• 2020

The design of large complex mechanical systems, such as automobile, aircraft, and ship, is a kind of Multidisciplinary Design Optimization (MDO) because it requires both experience and expertise in many areas. With the rapid development of technology and the demand to improve human convenience, the complexity of these systems is increasing further. The design of such a complex system requires an integrated system design, i.e., MDO, which can fuse not only domain-specific knowledge but also knowledge, experience, and perspectives in various fields. In the past, the MDO relied heavily on the designer's intuition and experience, making it less efficient in terms of accuracy and time efficiency. Process integration and the design optimization framework mainly support MDO owing to the evolution of IT technology. This paper examined the procedure and methods to implement an efficient MDO with reasonable effort and time using RCE, an open-source PIDO framework. As a benchmarking example, the authors applied the proposed MDO methodology to a bulk carrier's conceptual design synthesis model. The validity of this proposed MDO methodology was determined by visual analysis of the Pareto optimal solutions.

• Journal of Distribution Science
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• v.11 no.5
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• pp.55-62
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• 2013

Purpose - This study aims to determine the problems and limitations of the Commercial Area Activation System, which was created by a special law for promoting traditional markets and shopping districts to revitalize and efficiently manage the central commercial area in different regions. We also suggest different options for its improvement. Research design, data, and methodology - We also look into the problems of which is being promoted as a demonstration project, from the aspects of legal text and guidelines. Results - The current commercial area activation system has several problems. First, the establishment of a comprehensive basic plan on the commercial area activation is not a requirement. Second, the benefit principle should be established to prevent the moral laxity of merchants who serve important roles in the main components of the commercial area activation business when they conduct their business. Third, the current special law constrains the commercial management organization, as under the civil law yields a limitation on finding a profitable business model. Fourth, to efficiently, constructing a system that links the other central government businesses and is needed. into a regional development budget or a budget for funding small businesses that the central government can control, which is effective. Further, we offer some suggestions for medium- and long-term policies. First, an integrated coordination mechanism at the central office level should be installed while setting the basic policy to revitalize the Based on this policy, local governments need a system that exclusively based on the after establishing a comprehensive plan for urban regeneration and getting approval from the integration organization. Second, a system that enables an understanding of the problems with business promotion by monitoring the procedure of supporting projects and regularly assessing business achievements is needed. Third, a plan is needed for resolving conflicts between various interested parties that adopts the commercial area activation system for carrying out a total redevelopment of the commercial area where small shops are densely located. A market maintenance project has been conducted as a means to recover our traditional market, which was economically depressed, and to revive the local economy, but it is mostly conducted in the form of reconstruction or redevelopment and represents the interests of landowners and merchants. Thus, it is most likely to lead to a gradual disappearance of traditional markets. Conclusions - This study looks primarily into the problems that appeared in the legal text or the guidelines regarding the direction of improvement of the commercial area activation business that has been going on as a demonstration project since 2011 and suggests some solutions.

• Industry Promotion Research
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• v.5 no.2
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• pp.93-103
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• 2020

The purpose of this study is to provide a basis for the sustainability of cooperatives, To provide theoretical support for healthy development and academic research. The purpose of this study is to improve people's understanding of cooperatives (farmer professional cooperatives) in China. First of all, we analyze the background and concept of the introduction of cooperatives and the use of the latest advance research in China and South Korea; Secondly, we propose the status of Chinese cooperatives and the growth process of the legal system; Thirdly, we focus on the law and system of Chinese farmer professional cooperatives. Therefore, this study proposes the following four enlightenments : First, China should continue to improve and improve its legal systems, such as the tax assistance legal system, financial preferential legal system, and technical assistance legal system. Secondly, it is necessary to strengthen government supervision of cooperatives, improve the internal system of farmer cooperatives, improve the financing policies of farmer cooperatives, grasp the relationship between development and regulation, and form a development model centered on farmers. Third, the development of farmer cooperatives must be integrated with the local culture. Fourth, we must strengthen the training of cooperative members and strive to improve the level of management and self-innovation.

• Journal of the Institute of Electronics Engineers of Korea SD
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• v.48 no.7
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• pp.37-47
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• 2011

Real picture like high quality computer graphic is widely used in various fields and shader processor, a key part of a graphic processor, has been advanced to programmable unified shader. However, The existing graphic processors have been optimized to commercial algorithms, so development of an algorithm which is not based on it requires an independent shader processor. In this paper, we have designed and implemented a control unit to support high quality 3 dimensional computer graphic image on programmable integrated shader processor. We have done evaluation through functional level simulation of designed control unit. Hardware resource usage rate are measured by implementing directly on FPGA Virtex-4 and execution speed are verified by applying ASIC library. the result of an evaluation shows that the control unit has the commands more about 1.5 times compared to the other shader processors that is a behavior similar to the control unit and with a number of processing units used in a shader processor, compared with the other processors, overall performance of the control unit is improved about 3.1 GFLOPS.

• Journal of the Korean Association of Geographic Information Studies
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• v.4 no.2
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• pp.17-26
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• 2001

The Purpose of this study is to provide an effective way of developing GIS to UIS at the local level. For this purpose, the study typologically reviews the developing processes of UIS and utilizes a case study as research method. As the case, the study selects three types of UIS such as FM based UIS(Kwacheon), Urban Planning based UIS(Cheungju), and LIS based UIS(Taegu Nam-Gu). Based on these three UISs, the study concludes as follows. First, the developing process of GIS to UIS began with isolated approach through integrated model and finally to internet UIS. Second, UISs follows similar processes to that of GIS construction. Third, local governments focus on the development of FM based UIS of which construction budget is financed by the central government. Based on these findings, the study provides policy alternatives for the successful construction of UIS.

• The Journal of the Korea Contents Association
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• v.11 no.11
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• pp.49-61
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• 2011

In recent, library is considered as an integrated knowledge convergence center that can respond to various requests about information service of users. Therefor it is necessary to establish a novel information system based on information communications technologies of the era. In other words, it is currently required to develop mobile information service available in portable devices such as smart phones or tablet PCs, and to establish information system reflecting cloud computing, SaaS, Annotation, and Library 2.0 etc. In this paper we design and implement a library information system using collective intelligence and cloud computing. This information system can be adapted for the varieties of mobile service paradigm and abruptly increasing amount of electronic materials. Advantages of this concept model are resource sharing, multi-tenant supporting, configuration, and meta-data supporting etc. In addition it can offer software on-demand type user services. In order to test the performance of our system, we perform an effectiveness analysis and TTA authentication test. The average response time corresponding to variance of data reveals 0.692 seconds which is very good performance in timing effectiveness point of view. And we detect maturity level-3 or 4 authentication in TTA tests such as SaaS maturity, performance, and application programs.

• Korean Journal of Agricultural and Forest Meteorology
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• v.21 no.4
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• pp.261-268
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• 2019

Climate change and drought stress are having profound impacts on crop growth and development by altering crop physiological processes including photosynthetic activity. But finding a rapid, efficient, and non-destructive method for estimating environmental stress responses in the leaf and canopy is still a difficult issue for remote sensing research. We compared the relationships between photochemical reflectance index(PRI) and various optical and experimental indices on soybean drought stress under climate change conditions. Canopy photosynthesis trait, biomass change, chlorophyll fluorescence(Fv/Fm), stomatal conductance showed significant correlations with midday PRI value across the drought stress period under various climate conditions. In high temperature treatment, PRI were more sensitive to enhanced drought stress, demonstrating the negative effect of the high temperature on the drought stress. But high CO₂ concentration alleviated the midday depression of both photosynthesis and PRI. Although air temperature and CO₂ concentration could affect PRI interpretation and assessment of canopy radiation use efficiency(RUE), PRI was significantly correlated with canopy RUE both under climate change and drought stress conditions, indicating the applicability of PRI for tracking the drought stress responses in soybean. However, it is necessary to develop an integrated model for stress diagnosis using PRI at canopy level by minimizing the influence of physical and physiological factors on PRI and incorporating the effects of other vegetation indices.

• Culinary science and hospitality research
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• v.18 no.4
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• pp.27-46
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• 2012

Organizational factors and personal traits are two elements of widely acknowledged relevance in employees' organizational outcomes in hotel industry. Personal traits especially need to be further examined as a consideration for employment. As one of the personal traits that provide capability to manage emotions, emotional intelligence is selected. The empirical objective of this study is to investigate the effects of emotional intelligence on job satisfaction and organizational commitment in a structural model. To conduct research questions, five star deluxe hotel employees in Korea are targeted to be surveyed. Descriptive statistics and multivariate analysis of variance, and structural equation modeling(SEM) are utilized employing SPSS and AMOS 4.0 to analyze the survey results. It was found that the components of perceiving emotions and understanding emotions predicted job satisfaction. Relatively perceiving emotions presented a higher impact on each dimension of job satisfaction. Satisfaction with co-workers and communication can also explain the level of hotel employees' organizational commitment. Broadly speaking, the results suggest that effective psychotherapeutic or reciprocative programs should be integrated into hotel training contents for emotional intelligence development.