• Title/Summary/Keyword: Inoue method

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Comparative Study on the Transport Characteristics of Canalicular Liver Plasma Membrane Vesicles Prepared by Two Different Methods (제조 방법에 따른 간 모세담관막 소포계의 수송 특성 비교)

  • Song, Im-Sook;Chung, Suk-Jae;Shim, Chang-Koo
    • Journal of Pharmaceutical Investigation
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    • v.29 no.1
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    • pp.13-19
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    • 1999
  • Canalicular liver plasma membrane vesicles (cLPM) were prepared according to two different methods (Inoue method and Meier method), and were evaluated for their protein yield, enzyme activity and transport characteristics. No difference was found between the methods in the protein yield (i.e., $0.14{\pm}0.031$ and $0.15{\pm}0.050$ mglg liver for Inoue method and Meier method, respectively). The activity of alkaline phosphatase, a marker enzyme of canalicular membrane, was significantly (P<0.05) higher in the vesicles of Meier method $(3.52{\pm}0.91\;mmol/mg/hr)$than in the vesicles of Inoue method ($2.28{\pm}0.94$ mmol/mg/hr) indicating that more purified cLPM were obtained from Meier method compared with Inoue method. ATP-dependent vesicular uptake of taurocholate and tributylmethylammonium (TBuMA) was observed for vesicles of both methods, and the kinetic parameters responsible for the transport were similar between the vesicles of both methods (for example, $V_{max}:$ 9.72 nmol/mg protein/30sec and $K_m:$ 0.63 mM for Inoue method; $V_{max}:$ 10.1 nmol/mg protein/30sec and $K_m:$ 0.70 mM for Meier method). A pH gradient dependent counter transport of TBuMA was also observed for both vesicles with similar kinetic characteristics. Either the uptake of taurocholate in the absence of ATP or that of TBuMA in the absence of pH gradient, which may represent passive diffusion of respective compound into the vesicles, was more rapid for the vesicles of Meier method than for the vesicles of Inoue method. For example, passive diffusion rate constants $(K_d)$ for TBuMA uptake into the vesicles were 0.00030 and 0.00052\;{\mu}l/mg$ protein/min for the vesicles of Inoue method and Meier method, respectively. It may indicate that more leaky vesicles are obtained form the Meier method compared with the Inoue method. These aspects together with the time necessary to prepare the vesicles (i.e., 8 hr for Inoue method and 23 hr for Meier method) should be considered before selecting an appropriate method for the preparation of cLPM.

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On the Mathematical Model for Estimating Manoeuvring Performance of Ships (선박의 조종성능평가를 위한 수학모델에 관한 연구)

  • 손경호
    • Journal of the Korean Institute of Navigation
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    • v.13 no.2
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    • pp.57-73
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    • 1989
  • This paper presents a practical method to predict the characteristics of ship manoeuvring motions. A attempt is made to calculate the manoeuvring motions utilizing principal particulars of ship hull, properller and rudder as basic input data. The mathematical models, which describe the ship manoeuvring motions, are developed on the basis of MMG(5), Inoue(17), Hooft(18) and so on. Calcuations of manoeuvring motions for three kinds of typical characteristics, namely turning motion, zig-zag manoeuvre response and steady turning performance, are carried out. In order to examine the validity of the calculation method of this paper, simulations are run for seven merchant ships employed by Inoue(4). The computed results by present method are compared with full scale trials and Inoue's calculations(4). It can be concluded that the calculation method proposed in this paper is useful and pwoerful for prediction of characteristics of ship manoeuvring motions at the initial design phase or the application study on manoeuvring motions.

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Improvement of Analysis of the Ship's Maneuverability at Early Design Stage (초기 계획 단계에서 선박조종성능 해석에 대한 정도향상 연구)

  • 이호영
    • Journal of the Korean Institute of Navigation
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    • v.21 no.2
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    • pp.11-17
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    • 1997
  • In this paper, we studied to improve Inoue's[1] and Kijima's[2] model used to predict ship's manoeuvrability in initial design stage. To perform this work, we had PMM(Planar Motion Mechanism) test and rudder open water test for 12 Models of low-speed blunt-ship which have horn type rudder and bulb in afterbody. Using MMG(Mathematical Modelling Group) model, we analyzed hydrodynamic and MMG experimetnal coefficidnts used to search sensitivities and to estimate ship's manoeuvrability, adn the results are performed by the regression analysis of principle parameters. We varified them to analyze ship's manoeubbrability , had simulate manoeubrability to check revised MMG model and compared them wiht Inoue's method. the result of PMM test and sea trial. Then it is found that the revised method can predict the maneuverability more accurately even for an unstable ship.

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Two Species of Genus Chordodes(Gordioidea, Nematomorpha) from Korea (한국산 흑연가시 속(유선형동물 문, 철선층 목)의 2종)

  • 백광민
    • Animal Systematics, Evolution and Diversity
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    • v.9 no.2
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    • pp.221-228
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    • 1993
  • This study on the genus Chordodes was based on the specimens collected from four areas in Korea during the period October 1988 to October 1992. As a result, a new species is described using a scanning electron microscope and conventional method, under the name of Chordodes koreensis. And It was identified that C. japonensis Inoue, 1952 is synonymous with C. ferganensis Kirjanova and Spiridonova, 1989. This genus is reported for the first time from Korea.

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Fault Detection by Using an Adaptive Observer

  • Inoue, A.;Deng, M.;Yoshinaga, S.
    • 제어로봇시스템학회:학술대회논문집
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    • 2005.06a
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    • pp.710-713
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    • 2005
  • In this paper, a design method to detect faults in plants with uncertainties is proposed. When a plant has faults, the plant will be corrupted by an unknown fault signal. In addition, the plant also includes uncertainties, such as disturbances and plant parameter deviations. In this case, the proposed method estimates the fault signal by using an adaptive observer. Numerical examples are given to demonstrate the validity of the proposed method.

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High performance torque control of induction motor by speed sensorless vector control

  • Harashima, Fumio;Kondo, Seiji;Inoue, Shuji
    • 제어로봇시스템학회:학술대회논문집
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    • 1990.10b
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    • pp.1410-1414
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    • 1990
  • A method of speed sensorless vector control of induction motor is proposed in this paper. This method uses the slip frequency estimated by only the primary voltage and current. As this slip frequency accords with the command value of it, the commanded primary frequency is controlled. The validity of the method is confirmed by the simulation and experimental results.

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EVALUATION OF HAIR DAMAGE BASED ON MEASUREMENTS OF LABILE PROTEIN

  • Inoue, Takafumi;Ito, Mayumi;Kizawa, Kenji;Iwamoto, Yoshimichi
    • Proceedings of the SCSK Conference
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    • 2003.09a
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    • pp.142-160
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    • 2003
  • Most consumers have noted hair damage following coloring treatments. Proper evaluation of hair bleaching products must be performed using quantitative assessments of hair damage, though they are difficult, because of the slight fluctuations in hair composition. In the present study, we utilized a sensitive evaluation method for hair damage and found that the amount of soluble protein fraction extracted from hair under a reducing condition, termed labile protein, dramatically increased after bleaching.(omitted)

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Carbon Nanotube Deposition using Helicon Plasma CVD at Low Temperature

  • Muroyama, Masakazu;Kazuto, Kimura;Yagi, Takao;Inoue, Kouji;Saito, Ichiro
    • 한국정보디스플레이학회:학술대회논문집
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    • 2003.07a
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    • pp.201-202
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    • 2003
  • We developed a novel growth method of aligned carbon nanotubes. Aligned carbon nanotubes are grown on a metal catalyst on a glass substrate using biased Helicon plasma chemical vapor deposition (HPECVD) of $CH_4/H_2$ gases from 400 C to 500 C. The Helicon plasma source is one of the high-density plasma sources and is promising for low temperature carbon deposition. A Ni film was used as a catalyst to reduce the activation energy of the nanotubes' growth. The carbon nanotubes were deposited on the nickel catalysis layer selectively.

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Heat Distribution Analysis of an End-Quenching Process Considering Latent Heat of Transformation (변태잠열을 고려한 담금과정의 열전도 해석)

    • Journal of the Korean Society of Manufacturing Technology Engineers
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    • v.7 no.3
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    • pp.79-84
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    • 1998
  • It is very difficult to analyze the transient temperature distribution during quenching of the steel because of coupled effects among temperature, structures and stresses. In this paper, using Inoue's equation of evolution and mixture rule, transient temperature distribution is calculated by the finite element method considering latent heat of transformation structure and temperature dependence of physical and mechanical prperties for the 0.45% carbon cylindrical steel bar with 40mm diameter and 20mm height during end-quenching.

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