• Asian-Australasian Journal of Animal Sciences
• /
• v.27 no.4
• /
• pp.511-517
• /
• 2014

This study examined the effects of bacterial inoculants on chemical composition and fermentation indices of barley silage. Barley forage (Youngyang) was harvested at 24% dry matter (DM) and wilted to 47.9% DM. The wilted barley forage was chopped to 3-5 cm length and applied with no inoculant (CON), L. plantarum ($1{\times}10^{10}cfu/g$, LP) or Effective Microorganisms ($0.5{\times}10^9cfu/g$, EM). Then the forages were ensiled in four replications for each treatment in 20 L mini silos and stored for 100 days. The contents of crude protein and ether extract were higher in CON silage ensiled for 100-d, while the contents of DM and crude ash were higher in EM silage (p<0.05). The contents of ADF, NDF and hemicellulose as well as the in vitro DM digestibility were not affected by microbial inoculation (p>0.05). The pH, ammonia-N concentration and lactate to acetate ratio were higher (p<0.05) in CON silage, while lactate concentrations were higher (p<0.05) in CON and LP silage. Acetate concentration and lactic acid bacteria was increased (p<0.05) by both inoculants (LP and EM), but propionate concentration and yeast was increased (p<0.05) by EM and LP, respectively. These results indicated that the fermentation quality of barley silage was improved by the application of bacterial inoculants.

• Journal of The Korean Society of Grassland and Forage Science
• /
• v.15 no.4
• /
• pp.303-309
• /
• 1995

An experiment was camed out in 1994 to determine the effects of curing periods and preservatives on the characteristics and forage quality of baled alfalfa(Medicago sativa L.) hay after 60 days of storage. The main plots consisted of the curing periods such as 5 and 7 days, and the subplots consisted of preservatives such as commercial propionic acid preservative, lactic inoculant and control. Temperature during storage remained lower in 7 days cured hay bale than in 5 days and didn't rise over $30^{\circle}C$ in 7 days cured hay bale. After storage, bale with 7 days curing remained in better condition by visual assessment and DM loss of the bale was 21.4% with 5 days curing and 11.8% with 7 days curing. ADF and NDF contents of 5 days cured baled hay were 43.0 and 56.1%. respectively, which were lower than those of 7 days cured baled hay by 1.3 and 1.4%, respectively(P< 0.05). ADF and NDF contents of preservative treated baled hay were higher than those of the control(P<0.05). According to RFV, forage quality of the control and inoculant treated baled hay of 5 days curing, and the control of 7 days curing was classified as 3rd grade, but other baled hay was classified as 4th grade. The total nitrogen content of 5 days cured baled hay was 1.84% and 7 days was 1.38%(P<0.05). Significant differences(P<0.05) in total N content of baled alfalfa hay were found among the preservatives. ADIN/total N of 5 and 7 days cured baled alfalfa hays was 28 and 26%. respectively.

• Microbiology and Biotechnology Letters
• /
• v.32 no.2
• /
• pp.172-178
• /
• 2004

This research is performed for a biological control of Chinese cabbage clubroot, we isolated an antagonistic bacterium AC-3 against Plasmodiophora sp., causal pathogens of cabbage clubroot. The isolated strain was identified as Streptomyces sp. by culture morphology, biochemical reactions, and homology research based on l6S rDNA sequences. Streptomyces sp. AC-3 produced chitinase (9.3 units/$m\ell$) in culture broth. So Plasmodiophora sp. mycelia changed abnonnal swelling, curling and branching mycelia by Streptomyces sp. AC-3 culture. In a field infected by Plasmodiophora sp., the treatment of a organic fertilizer added 2% Streptomyces sp. AC-3 microbial inoculant, it resulted in about 50% reducing the severity of cabbage clubroot significantly on cabbage plants compared with treated organic fertilizer plants. Additional disease such as sclerotinia rot, fusarium wilt and pythium rot were also significantly reduced by the treatment of the organic fertilizer added Streptomyces sp. AC-3 microbial inoculant.

• Journal of The Korean Society of Grassland and Forage Science
• /
• v.21 no.3
• /
• pp.169-174
• /
• 2001

This study was conducted to investigate the effects of feed resources and collection system of nonconventional agricultural roughages, and reduction of environment pollution by turning non-conventional agricultural roughages to feed resources and diversification of feed supply. And a series of experiment were conducted to determine the effect of moisture control material on quality of sweet potato stalk, peanut stalk, barley straw, the experiments were conducted at Grassland and Forage Crops Division, National Livestock Research Institute, RDA, Suwon in 1999 and 2000. The results obtained from these experiments are summarized as follows; Acid detergent fiber(ADF), neutral detergent fiber(NDF) and total digestible nutrient(TDN) of sweet potato were 38.8, 50.7 and 58.2% in the control silage, but that of rice straw added silage for moisture control were decreased to 41.5, 50.8 and 56.1%. ADF, NDF, CP(crude protein) and TDN of peanut stalk were 42.2, 49.6, 10.5 and 55.6% on the rice straw added silage for moisture control, and there were not affected by barely straw added silage for moisture control. And ADF, NDF and TDN content of barely straw increased 42.8, 65.8 and 55.1% on the water+inoculants treated silage. The results of this study indicate that non-conventional agricultural roughages to feed resources should be make for silage at silo after dry matter percentage(ab0ut 35%) control. Also, rice straw added silage for moisture control will improve the silage fermentation and conservation. (Key words : Feed resources, Non-conventional agriculture, Fermentation, Inoculant, Rice straw)

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.48 no.5
• /
• pp.361-368
• /
• 2003

The responses of five varieties and three cultivars of pea (Pisum sativum) to Rhizobium inoculation on nodulation, growth, nitrogenase activity, dry matter production and N uptake were investigated. The pea varieties were IPSA Motorshuti-l, IPSA Motorshuti-2, IPSA Motorshuti-3, BARI Motorshuti-l, BARI Motorshuti-2 and the cultivars were 063, Local small and Local white. Fifty percent seeds of each pea variety/cultivar were inoculated with a mixture of Rhizobium inoculants at rate of 15g/kg seed and the remaining fifty percent seeds were kept uninoculated. The plants inoculated with Rhizobium inoculant significantly increased nodulation, growth, nitrogenase activity, dry matter production and N uptake. Among the varieties/cultivars, BARI Motorshuti-l performed best in almost all parameters including nitrogenase activity of root nodule bacteria of the crop. There were positive correlations among the number and dry weight of nodules (r=$0.987^{**}$, $0.909^{**}$), nitrogenase activity of root nodule bacteria (r=$0.944^{**}$, $0.882^{**}$), dry weight of shoot (r=$0.787^{**}$, $0.952^{**}$), N content (r=$0.594^{**}$, $0.605^{**}$) and N uptake (r=$0.784^{**}$, $0.922^{**}$) by shoot both at flowering and pod filling stages of the crop, respectively. It was concluded that BARI Motorshuti-l in symbiotic association with Rhizobium inoculant performed best in recording nitrogenase activity, dry matter production and N uptake by pea.

• Journal of The Korean Society of Grassland and Forage Science
• /
• v.41 no.4
• /
• pp.259-266
• /
• 2021

The present study was aimed to estimate the effect of ensiling period and bacterial inoculants on chemical compositions and fermentation characteristics on rye silage harvested at delayed stage. Rye (Secale cereale L.) was harvested after 20 days of heading stage (29.4% dry matter, DM). The harvested rye forage was applied with different inoculants following: applications of distilled water (CON), Lactobacillus brevis (LBB), Leuconostoc holzapfelii (LCH), or mixture of LBB and LCH at 1:1 ratio (MIX). Each forage was ensiled into 20 L mini bucket silo (5 kg) for 50 (E50D) and 100 (E100D) days in triplicates. The E50D silages had higher in vitro digestibilities of DM (IVDMD, p<0.001) and neutral detergent fiber (IVNDFD, p=0.013), and lactate (p=0.009), and acetate (p=0.011) than those of E100D, but lower pH, lactic acid bacteria (LAB), and yeast. By inoculant application, LCH had highest IVDMD and IVNDFD (p<0.05), while MIX had highest lactate and lowest pH (p<0.05). The CON and LCH in E50D had highest LAB and yeast (p<0.05), whereas LBB in E100D had lowest (p<0.05). Therefore, this study concluded that LCH application improved the nutrient digesbility (IVDMD and IVNDFD) of lignified rye silage, and longer ensiling period for 100 days enhanced the fermentation characteristics of silage compared to ensiling for 50 days.

• Journal of Powder Materials
• /
• v.30 no.3
• /
• pp.203-209
• /
• 2023

Aluminum alloy-based additive manufacturing (AM) has emerged as a popular manufacturing process for the fabrication of complex parts in the automotive and aerospace industries. The addition of an inoculant to aluminum alloy powder has been demonstrated to effectively reduce cracking by promoting the formation of equiaxed grains. However, the optimization of the AM process parameters remains challenging owing to their variability. In this study, the response surface methodology (RSM) was used to predict the crack density of AM-processed Al alloy samples. RSM was performed by setting the process parameters and equiaxed grain ratio, which influence crack propagation, as independent variables and designating crack density as a response variable. The RSM-based quadratic polynomial models for crack-density prediction were found to be highly accurate. The relationship among the process parameters, crack density, and equiaxed grain fraction was also investigated using RSM. The findings of this study highlight the efficacy of RSM as a reliable approach for optimizing the properties of AM-processed parts with limited experimental data. These results can contribute to the development of robust AM processing strategies for the fabrication of high-quality Al alloy components for various applications.

• Animal Bioscience
• /
• v.35 no.9
• /
• pp.1379-1389
• /
• 2022

Objective: This study identified the major lactic acid bacteria (LAB) strains from different fermented total mixed rations (FTMRs) via metataxonomic analysis and evaluated the ability of their standard strain as ensiling inoculants for corn stover silage. Methods: The bacterial composition of eight FTMRs were analyzed by 16S rDNA sequencing. Corn stover was ensiled without LAB inoculation (control) or with 1×10⁶ cfu/g LAB standard strain (Lactobacillus vaginalis, Lactobacillus reuteri, Lactobacillus helveticus, or Lactobacillus paralimentarius) selected from the FTMRs or 10 g/t commercial silage inoculant (CSI) around 25℃ for 56 days. For each inoculation, a portion of the silage was sampled to analyze ensiling characteristics at time intervals of 0, 1, 3, 7, 14, 28, and 56 days, gas production (GP), microbial crude protein and volatile fatty acids as the measurements of rumen fermentation characteristics were evaluated in vitro with the silages of 56 days after 72 h incubation. Results: Lactobacillus covered >85% relative abundance of all FTMRs, in which L. pontis, L. vaginalis, L. reuteri, L. helveticus, and L. paralimentarius showed >4% in specific FTMRs. CSI, L. helveticus, and L. paralimentarius accelerated the decline of silage pH. Silage inoculated with L. paralimentarius and CSI produced more lactic acid the early 14 days. Silage inoculated with L. paralimentarius produced less acetic acid and butyric acid. For the in vitro rumen fermentation, silage inoculated with CSI produced more potential GP, isobutyric acid, and isovaleric acid; silage inoculated with L. helveticus produced more potential GP and isovaleric acid, silage inoculated with L. paralimentarius or L. reuteri produced more potential GP only. Conclusion: The standard strain L. paralimentarius (DSM 13238) is a promising ensiling inoculant for corn stover silage. The findings provide clues on strategies to select LAB to improve the quality of silage.

• Korean Journal of Organic Agriculture
• /
• v.8 no.1
• /
• pp.139-145
• /
• 1999

The experiment was conducted to determine the effects of Plant growth and the microbial distribution in soils treated with microbial products, Mity-GroTM. The results from this experiment were as follows ; 1. Plant height of Tomato and Pepper were significantly increased at 30 days after planted in soils treated with Mity-GroTM. Therefore, treatment of microbial products, Mity-GroTM, was considered to contribute the plant growth at early stage. 2. Microbial distribution in soils treated with Mity-GroTM was significantly changed at specific mcirobial population. However, The ratio of bacteria/actinomycetes in the plot treated with Mity-GroTM was significantly enhanced.

• Journal of Korea Technical Association of The Pulp and Paper Industry
• /
• v.34 no.2
• /
• pp.54-60
• /
• 2002

The Wastewater of toilet paper mill recycling recovered milk carton was used as a raw material for this study. According to the actual mill conditions, hydraulic retention time was adjusted to 12 hours and F/M (Food/Micro-organism) ratio was adjusted to 0.23. Temperature of aeration basin was varied from 2$0^{\circ}C$ to 5$0^{\circ}C$. The change of Micro-organisms and removal efficiency of pollutant were investigated at the varied temperature of basin. Aeration basin using high thermal microbial inoculants showed more removal efficiency of SS, COD than aeration basin using conventional microbial inoculants at high temperature. Floc consolidation of aeration basin using high thermal microbial inoculants added sludge was better than that of sludge from aeration basin using conventional microbial inoculants.