• Journal of Acupuncture Research
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• v.17 no.2
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• pp.231-246
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• 2000

Acupuncture has been used for treatment of numerous diseases, especially for pain control in the oriental culture. However, the mechanism of pain control by acupuncture was not clear so far. The present study was examined that the effects of electro-acupuncture (EA) applied to the acu-point of extra-segmental area on modulation of formalin induced pain in Sprague - Dawley rats. In order to apply EA to acu-points in the plantar area of right fore paws, a pair of teflon - coated stainless steel wires were implanted in HT 7 (shin-mun) and PC 7 (dae-neong) 5 days before behavioral test. A behavioral test was performed by means of video camera after injection of 5% formalin ($50{\mu}l$) into the lateral plantar region of left hind paw. EA was delivered by a constant current stimulator at 4~5 mA, 2 ms, and 10 Hz for 30 min. The electromyographic activities were recorded in the biceps femoris muscle under chloral hydrate anesthesia. Test stimuli with 1~9mA were applied to the sural nerve territory including the medial portion of the 4th toe and the lateral portion of the 5th toe. Behavioral responses including favoring, flinching and bitting were occured in the biphasic pattern, such as the lst phase (0~5 min) and the 2nd phase (20~45 min) after formalin injection. However, EA (4~5 mA, 2 ms, 10 Hz) significantly inhibited Che behavioral responses. EMG activities of flexor reflex had a latency of 100~300 ms and thresholds of test stimuli for EMG were 4~5 mA in normal rats. Injection of formalin decreased threshold of test stimuli and increased EMG activities for 2hrs after injection. However, EA significantly inhibited EMG activities of flexor reflex increased by formalin and recovered EMG evoked thresholds. These results suggest that contralateral extra-segmental EA inhibits the first and second phases of formalin induced pain but their mechanism be needed to examine additionally.

• The Korean Journal of Pharmacology
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• v.10 no.2
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• pp.13-23
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• 1974

Results of an experiment on the general behavior of rats in order to explore the possible pharmacological actions of Panax Ginseng upon the central nervous system can be summarized as follows: 1) The rats treated with Ginseng-saponin intraperitoneally with the doses of 50 or 100 mg/kg show the decreased sleeping component and increase in lying and grooming component and walking and rearing component of general behavior. 2) There are no difference between control group and saline treated group in pattern of general behavior components. 3) In the Ginseng-saponin treated groups, the amphetamine stimulated walking and rearing component of genenarl behavior are reduced but lying and grooming component are elevated. 4) Ginseng-saponin shows very low acute toxicity in mice. The $LD_{50}$ in mice of 24 hours after intraperitoneal injection was 795 mg/kg. From the above findings, it is to suggest that the Ginseng saponin might stimulate the central nervous system but its mode of action appears to be different from that of amphetamine. And the effects of Ginseng saponin may be varied depending on various experimental situation or condition of animals.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2002.10a
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• pp.603-607
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• 2002

Cavity pressure is a factor of what is occurring inside the mold and is used as one of the process parameters that control the overall injection molding cycle. The insight of cavity pressure is able to predict part quality and optimum process condition. In this paper, it is adapted ejector pin sensor to measure the cavity pressure and investigates the flow balance and the cavity pressure according to different runner thickness for adjusting the flow balance. Flow balance is very important to have not the poor results such as flash and warpage in the family mold. This paper predicted flow balance and cavity pressure using CAE analysis tool and compared with the test results. The results of analysis and test have a good agreement with the cavity pressure profile and flow pattern of the test.

• Animal cells and systems
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• v.9 no.3
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• pp.145-152
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• 2005

A retrograde tracer, WGA-apo-HRP-gold, was injected into midline thalamic nuclei and subsequently orexin-A immunostaining was performed on the tuberal region of the hypothalamus in order to investigate orexinergic projections to the midline thalamus. Injection site was targeted within one specific region, i.e., paraventricular, centromedian, rhomboid, reuniens, or intermediodorsal nucleus, but it proved to be either one or a combination of these thalamic nuclei. The distribution of WG/orexin-double-labeled neurons exhibited a general pattern in that the majority of labeled cells were observed within the ventral portion of the lateral hypothalamus as well as the perifornical nucleus (PeF). A small number of double-labeled cells were also observed at the dorsomedial nucleus, the area dorsal to the PeF, dorsal portion of the lateral hypothalamus, and the posterior hypothalamus. These orexin-immunoreactive neurons might have wake-related influences over a variety of functions related with midline thalamic nuclei, which include autonomic control, associative cortical functions, and limbic regulation.

• 제어로봇시스템학회:학술대회논문집
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• 2001.10a
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• pp.86.4-86
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• 2001

This paper presents the results of a visualization analysis of the correlation between the fiber orientation and flow pattern in injection molding using the Gate-magnetization method developed for the precise visualization of melt flow. The results of the comparisons of the fiber orientation angles with the flow patterns by the Gate-Magnetization method for GPPS mixed with glass fibers show the strong correlation between the flow patterns and fiber orientation angles. According to forward movement of the flow, the fiber orientation patterns move toward the side walls following the flow patterns. These results elucidate that fibers are oriented in the expansion process of the melt, and ...

• Journal of Korea Foundry Society
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• v.25 no.4
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• pp.168-172
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• 2005

In this study, the effects of chill layers occurred in shot sleeve on the molten metal filling were analyzed through computer simulation and the behavior of chill layers with temperature variation of shot sleeve set from 200 to $280^{\circ}C$ was also investigated. The simulation results showed the chill layers set in the in-gates during the injection process change the main filling direction and cause turbulent flow pattern, resulting in porosities inside the castings. The amount of chill layers with the increasing temperature of shot sleeve was considerably reduced. And particularly, at the setting temperature of $280^{\circ}C$ by heat control unit, the big reduction in chill layers, excellent trimmed surface and the highest densification were achieved, suggesting that as the optimal sleeve condition in diecasting, especially for the highly complex parts like valve body.

• Journal of Oral Medicine and Pain
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• v.22 no.2
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• pp.341-358
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• 1997

Clusterin is a highly glycosylated protein composed of two disulfide linked subunits. Although its biolobical action is not clearly defined, clusterin seems to be involved not only in remodeling of damaged tissue, but also in production of halitosis, the present study was designated to elucidate the expression of clusterin in the salivary gland of diabetic rats. For this study, 24 Sprague-Dawley rats were used for the experiment and divided into 2groups: control and experimental. The experimental group was composed of 18 rats and the control goup was 6 rats. After nduction of diabetes by STZ injection, the animals were sacrificed at 1,3,5,7,10,14 days. The parotid and submamndibular glands were observed histologically and the transcriptional expression of clusterin in the glands by Northern blot. The finding were as follows : 1. In experimental group, the salivary glands were observed at day 3 and then a seven destructive pattern was found in the glands at day 5. Howere, regeneration of gland tissue occured at day 14. 2. In experimental goup, destructive change was examined in the septal connective tissue after 7 days, and gradually more serious. 3. In experimental group, clusterin was expressed in the submandibular glands after 5 days, but in parotid glands to a lesser extent after 10 days. These results suggest that clusterin seems to be closely associated with histologic changes in the mucous glands rather serous glands.

• Transactions of the Korean Society of Automotive Engineers
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• v.22 no.2
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• pp.156-165
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• 2014

This paper is the first investigation on the effect of flow control methods on the part load performance in a spark ignition engine. For comparison of the methods, two control devices, port throttling and masking, were applied to a conventional engine without any design change of the intake port. Steady flow evaluation shows that steady flow rates per unit opening area and swirl ratio are very low compared with the port throttling and saturated from mid-stage valve lift, however, swirl increases slightly as the lift is higher in case of 1/4 masking control. In the part load performance, the effect of simple port throttling on lean misfire limit expansion is limited and insufficient; on the other hand a masking improves the limit considerably without any port modification for increasing swirl. Also the results show that the intake flow control improves the combustion with following two mechanisms: stratification induced by the combination of the flow pattern and the fuel injection timing attribute to ignition ability and the intensified flow ensure fast burn. In addition fuel consumption reduces under the flow controls and the reduction rate is different according to the operation conditions and control methods. At the Stoichiometric and/or low speed and low load the throttling method is more advantageous; however vice versa at lean and high load condition. Finally, the throttling is more efficient for HC reduction than masking, on the other side the NOx emissions increase under the masking and decrease under the port throttling compared with conventional port scheme.

• Korean Journal of Veterinary Research
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• v.57 no.2
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• pp.105-111
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• 2017

Hyaluronic acid (HA) has been investigated for biomedical and pharmaceutical applications. This study was conducted to determine the distributions of HA nanoparticles (NPs; size 350-400 nm) and larger HA polymers in mice at intervals after application. $^{177}Lutetium$ (Lu)-labeled HA-NPs or HA polymers were intravenously injected (5 mg/kg) into male ICR mice, and radioactivity levels in blood and target organs were measured from 0.25 h to 28 days post-injection. In blood, the radioactivities of HA-NPs and HA polymer peaked at 0.5 h after injection but were remarkably decreased at 2 h; subsequently, they maintained a constant level until 6 days post-injection. HA-NPs and HA polymers were observed in the liver, spleen, lung, kidney, and heart (in ascending order) but were seldom observed in other organs. After 3 days, both the HA-NP and HA polymer levels showed similar steady decreases in lung, kidney, and heart. However, in liver and spleen, the HA-NP levels tended to decrease gradually after 1 day and both were very low after 14 days, whereas the HA polymer level accumulated for 28 days. The results indicate that HA-NPs, with their faster clearance pattern, may act as a better drug delivery system than HA polymers, especially in the liver and spleen.

• Clinical and Experimental Reproductive Medicine
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• v.28 no.3
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• pp.199-207
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• 2001

Objective: The present study was undertaken to examine the effects of magnesium ion in the culture medium on the development of mouse fertilized oocytes either before or after pronuclear formation, and to investigate whether the effect of magnesium ion is related with the redistributional change of mitochondria. Methods : Fertilized oocytes obtained from the oviducts of mice at 15 hr after hCG injection before pronuclear formation (pre-PN) or 21 hr after hCG injection after pronuclear formation (post-PN) were used. The embryos were cultured for 3 days with basic T6 medium-magnesium free and various concentrations of magnesium ion, 0.0, 0.5, 1.0, 2.0, 4.0 or 8.0 mM, respectively. After culture, the developmental stages of embryos and the number of nuclei were evaluated. To observe the effects of magnesium ion on the mitochondrial distribution, fertilized oocytes were collected at 21 hr after hCG injection and cultured for 6 hr with various concentration of magnesium ion. As a control, fertilized oocytes with pronuclei at 27 hr after hCG injection were used. Results: The concentration of magnesium ion to accelerate the in vitro development of mouse fertilized oocytes appeared to be at 2.0 mM for the pre-PN and the post-PN stage embryos. In the mitochondrial redistribution patterns, the embryos cultured in 2.0 mM concentration of magnesium ion showed the highest percentage (22.6%) of distinct perinuclear clustering pattern comparing to other experimental group. Conclusion: The effect of magnesium ion may be related to the cytoplasmic redistribution of mitochondria. This relationship seems to connect the developmental competence of preimplantation mouse embryos in vitro. These results can suggest that higher concentration of magnesium ion (2.0 mM) than those of conventional culture medium ($0.2{\sim}1.2\;mM$) is more suitable for in vitro culture of preimplantation mouse embryos.