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Contamination of Green Vegetable Juice by E. coli O157:H7 during Storage (E. coli O157:H7에 의한 녹즙 저장 환경에서의 미생물학적 오염도 조사)

  • Lim, Eun Seob;Koo, Ok Kyung
    • Korean Journal of Food Science and Technology
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    • v.47 no.4
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    • pp.446-451
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    • 2015
  • The market for green vegetable juice (GVJ) is growing owing to the increasing demand for healthy food; however data on the safety and quality of GVJ are poorly reported. The objective of this study was to investigate the change in microbial community in GVJ during storage and its contamination by E. coli O157:H7. The microbial community was analyzed via culturable and non-culturable methods at 5, 10, and $25^{\circ}C$ for different storage times. In the non-culturable method, denaturing gradient gel electrophoresis (DGGE) was used. The initial bacterial concentration was $2.92{\times}10^5CFU/mL$, which exceeded the limit prescribed by the Korean Food Hygiene law. The results of the DGGE analysis indicated that the microbial community during storage was diverse and the spoilage lactic acid bacteria were prevalent at a later stage. Other bacteria such as Rahnella, Citrobacter, Pseudomonas, and Cyanobacteria were identified. Thus, the results strongly emphasize the need to pay attention to GVJ production safety, especially with respect to temperature control, in order to prevent the growth of foodborne pathogens such as E. coli O157:H7 and other spoilage bacteria.

Recent Research Trends and Implications of the Social Service Supply System (사회서비스 공급체계의 최근의 연구 동향)

  • Seo, Jeong-Min;Kim, Nang-Hee
    • Journal of Digital Convergence
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    • v.14 no.4
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    • pp.55-68
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    • 2016
  • Academic research of the social service delivery system is very limited. This paper checked on the status of research on current social service delivery system in order to explore new research and policy directions. Analysis study consisted of 79 pieces and the framework was divided into dimensional analysis, content analysis and analytical standards. Research on the papers of social service delivery systems showed from the initial stage of the policy discussion to the phase of the individual services. Summarizing the study is as follows. First, This studies confirmed that at this time the quality studies on social service delivery system is needed. Second, this indicates that right now, the government budget of social services and the data relating to social service agencies will be opened to the public for the expansion of social services studies. Third, to expand social services delivery systems research, it showed that social services demand forecasting research such as budget allocation, population characteristics, equality of public service and local information is needed. Finally, since the quality of service is reduced by the oversupply, the discussion of the entry regulations of social service providers is required.

Screening of Potential Anticancer Compounds from Marketed Drugs: Aripiprazole, Haloperidol, Miconazole, and Terfenadine Inhibit Cytochrome P450 2J2 (시판 약물의 시토크롬 2J2 약물대사효소 저해능 탐색)

  • Liu, Kwang-Hyeon
    • Journal of Life Science
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    • v.21 no.11
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    • pp.1558-1564
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    • 2011
  • Cytochrome P450 2J2 (CYP2J2) plays important roles in the metabolism of endogenous metabolites such as arachidonic acid as well as therapeutic drugs. CYP2J2 is overexpressed in human cancer tissues and cancer cell lines, as well as in epoxyeicosatrienoic acids (EETs) and CYP2J2-mediated metabolites, and prevent apoptosis of cancer cells. This study aimed to screen marketed drugs for inhibitory potential on CYP2J2 isoforms using human liver microsomes. The initial screen isolated 4 compounds, from 120 marketed drugs, that inhibited the CYP2J2-mediated astemizole O-demethylation more than 50% in the following the order: haloperidol (75%) > terfenadine (56%) > aripiprazole (55%) > miconazole (52%). Miconazole strongly inhibited CYP2J2-mediated ebastine hydroxylation ($IC_{50}$=11.2 ${\mu}M$) and terfenadine hydroxylation ($IC_{50}$=2.2 ${\mu}M$), and terfenadine also inhibited CYP2J2-mediated ebastine hydroxylation ($IC_{50}$=13.6 ${\mu}M$) in a dose dependent manner. The present data suggest that these drugs are potential candidates for further evaluation for their anti-cancer activities.

A Study on the Adsorptive Removal of Heavy Metals Using Inflated Vermiculites (팽창질석을 이용한 중금속 흡착제거에 관한 연구)

  • Lee, Junki;Koh, Taehoon;Kim, Sukyung;Lee, Taeyoon
    • Journal of the Korean GEO-environmental Society
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    • v.10 no.6
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    • pp.61-68
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    • 2009
  • The main objective of this study was to examine the removal of heavy metals from water by inflated vermiculites. The component of vermiculites was analyzed by XRF, and the concentration of metal ion was measured by ICP-AES. Serial batch kinetic tests and batch sorption tests were conducted to determine the removal characteristics for heavy metals in aqueous solutions. As a result, solution pH values of tests with the inflated vermiculites generally increased and then stabilized. Equilibrium pHs were generally established within 5 hrs. In addition, removal rates of inflated vermiculites were tested at the initial concentration of 3 mg/L. As a result, at equilibrium concentration, except for chromium (36.23%), Most of the heavy metals were effectively removed (96.08~98.54%). Finally, sorption data were correlated with both Langmuir and Freundlich isotherms. The Qmax obtained from Langmuir isotherm were determined to Pb $725.4mg\;kg^{-1}$, Cd $568.8mg\;kg^{-1}$, Zn $540.2mg\;kg^{-1}$, Cu $457.2mg\;kg^{-1}$ Cr $0.9mg\;kg^{-1}$ respectively. The results of the study indicate that inflated vermiculites can be properly used as an adsorbent for various heavy metals because of its outstanding removal rate.

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A retrospective clinical study of survival rate of the ITI $TE^{(R)}$ implant (ITI $TE^{(R)}$ 임플란트의 생존율에 관한 후향적 임상 연구)

  • Suh, Hyun-Kee;Chae, Gyung-Joon;Jung, Ui-Won;Kim, Chang-Sung;Cho, Kyoo-Sung;Choi, Seong-Ho;Chai, Jung-Kyu;Kim, Chong-Kwan
    • Journal of Periodontal and Implant Science
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    • v.36 no.3
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    • pp.673-682
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    • 2006
  • Recent study shows that implant design has a great impact on initial stability in bone. The ITI $TE^{(R)}$ implant, designed originally for immediate placement has a tapered/ cylindrical form which fits the anatomical shape of the natural alvelous or tooth root. The increased diameter at the collar region coupled with more threads lead to more bone contact and enhanced stability. The aim of this retrospective study is to evaluate the clinical use and the efficacy of recently introduced ITI TE implant with a new macro-design. The following results are compiled from 139 patients who received ITl TE implant surgery at the periodontal department. of Yonsei University Hospital between July 2002 and September 2005. 1. 139 patients received 173 ITl $TE^{(R)}$ implants in their maxilla and mandible (Mx 82, Mn 91). Posterior area accounted for 84% of the whole implant surgery, 2. In the distribution of bone quality, type III(41,0%) was the most, followed by type IV(41,0%) and type II (27.7%). As for the bone quantity, type B(43.9%) was the most, followed by type C(42.2%), type D(12.2%) and type A(1.7%). 3. 125 implants(83.9%) were treated by single crown, which accounted for the majority. 4, The total implant survival rate was 100% after a mean follow-up period of 21.2 months. This preliminary data with ITl $TE^{(R)}$ implant showed excellent survival rate although the majority of implants evaluated in this study were placed in the posterior region of the jaw and compromised sites.

Estimation of In-plant Source Term Release Behaviors from Fukushima Daiichi Reactor Cores by Forward Method and Comparison with Reverse Method

  • Kim, Tae-Woon;Rhee, Bo-Wook;Song, Jin-Ho;Kim, Sung-Il;Ha, Kwang-Soon
    • Journal of Radiation Protection and Research
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    • v.42 no.2
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    • pp.114-129
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    • 2017
  • Background: The purpose of this paper is to confirm the event timings and the magnitude of fission product aerosol release from the Fukushima accident. Over a few hundreds of technical papers have been published on the environmental impact of Fukushima Daiichi accident since the accident occurred on March 11, 2011. However, most of the research used reverse or inverse method based on the monitoring of activities in the remote places and only few papers attempted to estimate the release of fission products from individual reactor core or from individual spent fuel pool. Severe accident analysis code can be used to estimate the radioactive release from which reactor core and from which radionuclide the peaks in monitoring points can be generated. Materials and Methods: The basic material used for this study are the initial core inventory obtained from the report JAEA-Data/Code 2012-018 and the given accident scenarios provided by Japanese Government or Tokyo Electric Power Company (TEPCO) in official reports. In this research a forward method using severe accident progression code is used as it might be useful for justifying the results of reverse or inverse method or vice versa. Results and Discussion: The release timing and amounts to the environment are estimated for volatile radioactive fission products such as noble gases, cesium, iodine, and tellurium up to 184 hours (about 7.7 days) after earthquake occurs. The in-plant fission product behaviors and release characteristics to environment are estimated using the severe accident progression analysis code, MELCOR, for Fukushima Daiichi accident. These results are compared with other research results which are summarized in UNSCEAR 2013 Report and other technical papers. Also it may provide the physically based arguments for justifying or suspecting the rationale for the scenarios provided in open literature. Conclusion: The estimated results by MELCOR code simulation of this study indicate that the release amount of volatile fission products to environment from Units 1, 2, and 3 cores is well within the range estimated by the reverse or inverse method, which are summarized in UNSCEAR 2013 report. But this does not necessarily mean that these two approaches are consistent.

Cross-layer Design of Routing and Link Capacity Extension for QoS in Communication Networks (통신망 QoS를 위한 라우팅과 용량 증설의 계층간 최적화 기법)

  • Shin, Bong-Suk;Lee, Hyun-Kwan;Park, Jung-Min;Kim, Dong-Min;Kim, Seong-Lyun;Lee, Sang-Il;Ahn, Myung-Kil
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.35 no.12B
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    • pp.1749-1757
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    • 2010
  • This paper considers the cost minimization problem to satisfy QoS (Quality of Service) requirements for a given network, in particular when communication resources to each link can be additionally assigned. For the purpose of quantifying QoS requirements such as data transfer delay and packet loss, we introduce the cost function considering both the link utilization factor and the additionally assigned resource. To minimize this cost function, we firstly formulate a Basic Capacity Planning (BCP) problem, a special case of Network Utility Maximization (NUM). We show that the solution of this BCP problem cannot be optimal via a counter example. In this paper, we suggest the cross-layer design of both additionally assigned resource and routing path, whose initial values are set to the result of BCP problem. This cross-layer design is based on a heuristic approach which presents an effective way to plan how much communication resources should be added to support the QoS requirements in future. By simulation study, we investigate the convergence of the cost function in a more general network topology as well as in a given simple topology.

Comparison of KTAS(Korean Triage and Acuity Scale) results by Triage Classifier (중증도 분류자 직종에 따른 중증도 분류 결과의 차이 비교)

  • Huh, Young-Jin;Oh, Mi-Ra;Kim, Se-Hyung;Han, So-Hyun;Pak, Yun-Suk
    • Journal of Convergence for Information Technology
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    • v.10 no.4
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    • pp.98-103
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    • 2020
  • The purpose of this study was to determine whether the results of KTAS(Korean Triage and Acuity Scale) triage classifier differ according to the occupations. We analyzed a total of 10,960,359 cases of data sent to the NEDIS from January 1st, 2016 to December 31th, 2017. The triage classifier were MD(Medical Doctor), R(Resident), INT(Intern), GP(General Practitioner), RN(Registered Nurses) and EMT(Emergency Medical Technician). The consistency between the initial triage and final triage results was the highest GP(98.9%) and the lowest INT(80.2%). The results of over-triage classification was the lowest by GP(0.6%) and the highest for INT(16.0%). Also, the results of under-triage classification was the lowest by MD, EMT(0.4%) and the highest for INT(3.8%). The results of KTAS triage classifier significantly differ from according to the occupations(p<0.001). Triage classification should not differ from according to occupations and skill. It is necessary to strengthen the classifier's capacity for accurate triage classifications.

Marine Controlled-source Electromagnetic Surveys for Hydrocarbon Exploration (탄화수소 탐지를 위한 해양 인공송신원 전자탐사)

  • Kim, Hee-Joon;Han, Nu-Ree;Choi, Ji-Hyang;Nam, Myung-Jin;Song, Yoon-Ho;Suh, Jung-Hee
    • Geophysics and Geophysical Exploration
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    • v.9 no.2
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    • pp.163-170
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    • 2006
  • The shortage of proven hydrocarbon reserves has resulted in exploration progressing from the offshore into progressively deeper water of the continental shelf. Despite the success of seismic acquisition at ever greater depths, there are marine geological terrenes in which the interpretation of seismic data is difficult, such regions dominated by scattering or high reflectivity that is characteristic of carbonate reefs, volcanic cover and submarine permafrost. A marine controlled-source electromagnetic (CSEM) method has recently been applied to the oil and gas exploration thanks to its high-resistivity characteristics of the hydrocarbon. In particular, this method produces better results in terms of sensitivity under the deep water environment rather than the shallow water. Only in the last five years has the relevance of CSEM been recognized by oil companies who now use it to help them make exploration drilling decisions. Initial results are most promising and several contractors now offer magnetotelluric and CSEM services.

Prediction of weight loss of low temperature storage tomato (Tiwai 250) by non-destructive firmness measurement (비파괴적인 경도 측정을 통한 저온저장 토마토(티와이250)의 감모율 예측)

  • Cui, Jinshi;Yoo, Areum;Yang, Myongkyoon;Cho, Seong In
    • Food Science and Preservation
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    • v.24 no.2
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    • pp.181-186
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    • 2017
  • This study was conducted to investigate the weight loss, firmness, external color and vitamin C (VC) content of tomatoes (Lycopersicon esculentum) using non-destructive method to measure identical tomato samples during 15 days storage at low temperature and high humidity. Tomatoes were harvested at the light red stage, sorted, box packed and then stored in thermo-hygrostat ($10{\pm}1^{\circ}C$, $90{\pm}10%RH$). The quality changes in weight loss, firmness and external color were measured every 3 day interval. Weight loss was increased by $1.13{\pm}0.15%$, but it may not be considered to affect quality. Surface color of fruit was changed, especially in lightness and hue angle value. The color values were analyzed by analysis of variance (ANOVA), and the results were significant (p<0.001). Firmness of fruit declined during storage, but it did not decrease in direct proportion. On the storage of day 15, firmness was decreased to 40% of initial state. At last, all the experiment data are summarized and the relationship between firmness and weight loss is analyzed to construct a linear regression mathematical model that can predict the weight loss with the firmness value measured by non-destructive method. This research result could be useful in helping tomato exporters and suppliers to get real-time quality factor by using proposed method and regression model.