• Ocean and Polar Research
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• v.40 no.2
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• pp.69-75
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• 2018

We investigated the effects of environmental variations on the growth and survival rate of Stichopus japonicus to determine the optimum environmental conditions for its growth. Literature studies and a 12 month-long diver survey were carried out to understand the habitat, ecology and size of the surveyed area. Based on the collected data, we suggested optimum habitat conditions for releasing S. japonicus. Experiments on releasing S. japonicus were conducted in the breakwater of the Hwagye fishing cooperative in Hwagey-ri, Namhae-un, Gyeongsangnam-do, Korea. To implement the experiments, we divided the surveyed area into 4 sub-areas with different characteristics: (1) sand and silt zone; (2) artificial sea cucumber bank zone; (3) artificial rock bank zone; and (4) marine algae zone. The experiment lasted for 12 months. We released 32,000 sea cucumbers over $120m^2$ of each of the sand and silt zone, artificial rock bank zone and marine algae zone and released 6,000 sea cucumbers over $120m^2$ of the artificial sea cucumber bank zone. The average density of the released sea cucumbers from day 30 to day 360 after the releasing was conducted was the highest in the artificial sea cucumber bank zone ($23.7animal/m^2$), which was followed by artificial rock bank zone ($2.0animal/m^2$), marine algae zone ($1.9animal/m^2$) and sand and silt zone ($0.8animal/m^2$). The analysis on growth showed that the initial average weight of 2.3 g increased on day 360 after the releasing to 12.5 g in the artificial sea cucumber rank zone, 20.2 g in the sand and silt zone, 23.3 g in the artificial rock bank zone and 22.9 g in the marine algae zone. Results from the experiment along with the literature analysis suggest the following optimum habitat conditions: $10-15^{\circ}C$ water temperature; 28-34 psu salinity; 5-10 m water depth; 0.2-0.5 m/s velocity; rock, stone and muddy sand as substrate; and less than 20% mud in the substrate.

• Journal of Pharmaceutical Investigation
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• v.26 no.1
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• pp.33-41
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• 1996

In order to study the effect of phenobarbital(PB) on the hepatic transport of diltiazem(DTZ), $Ca^{2+}$ channel blocker, we used isolated hepatocytes of rat which was intraperitoneally pretreated with phenobarbital sodium(75 mg/kg) for four days once a day. For the isolation of rat liver cells, a modification of the two step procedure of Seglen was used. DTZ was dissolved in incubation buffer to the final DTZ concentrations of 200, 400, 600, 800 and 1000 ng/ml in order to elucidate the uptake characteristics of DTZ by hepatocytes. Reactions were stopped at 10, 20, 30, 45, 60, 90, 120 and 300 sec. The initial velocity was determined by disappearance of diltiazem in the hepatocyte suspension. On the other hand, to determine the effect of PB on the in vitro hepatic intrinsic clearance of DTZ we obtained the metabolism rates of DTZ in the control and the PB-pretreated rat hepatocyte at various time intervals. According to pretreatment with PB, the size of hepatocyte and the amount of protein per $10^6$ cells were significantly (p<0.01) increased from $26.92{\pm}0.1364\;m$ to $35.31{\pm}1.00\;m$ and from $468{\pm}6.5\;{\mu}g/10^6$ cells to $628.8{\pm}12.1{\mu}g/10^6$ cells, respectively. In the case or hepatic uptake of diltiazem, $K_m$ was not different in the normalization by cell numbers and increased from $2.90\;{\mu}M\;to\;13.89\;{\mu}M$ in the normalization by protein amount. $V_max$ was increased regardless of normalization by protein amount and cell numbers, from $1.21\;{\mu}mole/min \;{\cdot}\;mg\;protein\;to\;3.96\;{\mu}mole/min\;{\cdot}\;mg\;protein\;and\;from\;2.38\;{\mu}mole/min\;{\cdot}\;10^6\;cells\;to\;2.83\;{\mu}mole/min\;{\cdot}\;10^6\;cells$, respectively. The in vitro hepatic intrinsic clearance of DTZ was significantly (p<0.01) increased from $0.640{\pm}0.038\;ml/mim\;{\cdot}\;10^6\;cells\;to\;2.385{\pm}0.212\;ml/min\;{\cdot}\;10^6\;cells$ due to PB-pretreatment. These results suggest that the uptake of DTZ by hepatocyte is extremely fast and PB enhances the hepatic intrinsic metabolic clearance of DTZ.

• Journal of the Korean earth science society
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• v.30 no.6
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• pp.787-798
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• 2009

A new apparatus was presented in order to help understand the concept of the Coriolis force which is essential in understanding the trajectories of the atmospheric current and the tide of seawater. In the apparatus proposed in high-school textbooks, since the slide from which the ball is released is set outside the rotating disk, it was not possible to interpret, with the trajectory of the ball on the disk, the motion of the atmosphere and the current of seawater occurring as a result of the Coriolis force. In order to resolve such problem, a new apparatus was developed in which the slide was set on the disk and rotated with the disk. Experiments were carried out using both the existing apparatus and the new apparatus, and the results were compared. While, in the experiment performed with the existing apparatus, it was difficult to analyze the trajectory of ball because the motion of the ball was not smooth when it hit the disk, in the experiment with the new apparatus it was much easier to analyze the trajectories. It was also possible to compare the trajectories when the initial velocity of the ball was varied.

• Korean Journal of Food Science and Technology
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• v.32 no.1
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• pp.140-146
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• 2000

The physicochemical properties, such as retrogradation, fat acidity and viscosity, on the pine nut's gruel at various contents of pine nut (0, 1, 3 and 5%) during storage at 4, 25 and $40^{\circ}C$ were investigated. The degree of retrogradation was increased rapidly at the initial storage stage. The lowest value (about 10%) of the degree of retrogradation was observed in the gruel sample containing 3% of pine nut. Also, the rate constant of retrogradation was showed the lowest value (0.0422) in the gruel containing 3% of pine nut regardless of storage temperatures. The fat acidity was showed the lower value than 30 mg KOH in all samples. The viscosity increasing velocity(RVU/min) between holding strength and final viscosity was decreased as the pine nut's content was increased. However, it was not affected by the storage temperatures.

• Membrane Journal
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• v.15 no.4
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• pp.338-348
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• 2005

The permeate flux decline on microfiltration capillary membrane operation was investigated with inorganic colloidal solutions. The permeate flux of the alumina solution is two times higher in average than that of the bentonite solution. The flux decline with increase in operation time was less in the alumina solution than in the bentonite solution. The rate of initial flux decline until 10 min was higher on the bentonite solution over the alumina solution. The decline in permeate flux was due to both the cake formation and the pore blocking. The latter effect was higher in the operation of the bentonite solution. In comparing the ratio of each fouling component to the total fouling fur the $1.0\;kg_f/cm^2$ TMP condition, complete blocking was $9.35\%$, standard blocking was about $6.82\%$ and cake filtration was $83.83\%$, respectively. With the increase in cross flow velocity, the permeate flux increased by $6.0\%$ for the alumina solution and by 14.0 for the bentonite solution. With the increase in average pore size fromm $0.24\;{\mu}m\;to\;0.34\;{\mu}m$, the permeate flux increases 1.61 times for the alumina solution and 1.76 times far the bentonite solution.

• Journal of the Korea Concrete Institute
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• v.13 no.4
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• pp.336-345
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• 2001

The construction of underwater structures has been increased, but underwater concrete hassome problems of quality deterioration and contamination around in-situ of civil and architecture; therefore, new materials and methods for them are demanded. In this paper in-situ application of underwater antiwashout concrete which is manufactured for trio purpose of not only decreasing suspended solids and the heat of hydration but also increasing long term strength was studied. In the case of mock-up test(Ⅰ), when underwater antiwashout concrete, whose slump flow was 58 cm, was placed in the mock-up test at a speed of 24 ㎥/hr, it took about a minute to flow to the side wall, and the surface was maintained at horizontal level. In this case, compressive strength of the core specimens in each section was higher than the standard design compressive strength of 240 kgf/㎠. In the case of mock-up test(II), pH value and suspended solids of high strength underwater antiwashout concrete were 10.0∼11.0 and 51 mg/ℓ at 30 minutes later, initial and final setting time were about 30, 37 hr, and the slump flow of that was 53$\pm$2 cm. In the placement at a speed of 27 ㎥/hr, there was no large difference in flowing velocity, with or without reinforcement and flowing slope was maintained at horizontal level. In this case, compressive strength and elastic modulus of the core specimens somewhat decreased as flowing distance was far : however, those of central area showed the highest value.

• Journal of the Korea Convergence Society
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• v.11 no.3
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• pp.53-59
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• 2020

Initial response is important in marine oil spills, such as the Hebei Spirit oil spill, but it is very difficult to predict the movement of oil out of the ocean, where there are many variables. In order to solve this problem, the forecasting of oil spill has been carried out by expanding the particle prediction, which is an existing study that studies the movement of floats on the sea using the data of the float. In the ocean data format HDF5, the current and wind velocity data at a specific location were extracted using bilinear interpolation, and then the movement of numerous points was predicted by particles and the results were visualized using polygons and heat maps. In addition, we propose a spill oil particle matching algorithm to compensate for the lack of data and the difference between the spilled oil and movement. The spilled oil particle matching algorithm is an algorithm that tracks the movement of particles by granulating the appearance of surface oil spilled oil. The problem was segmented using principal component analysis and matched using genetic algorithm to the point where the variance of travel distance of effluent oil is minimized. As a result of verifying the effluent oil visualization data, it was confirmed that the particle matching algorithm using principal component analysis and genetic algorithm showed the best performance, and the mean data error was 3.2%.

• Journal of Navigation and Port Research
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• v.34 no.5
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• pp.349-354
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• 2010

Cheonan, Republic of Korea Navy patrol ship sank had happened by an unknown incident in the vicinity of Baekryeongdo southwest 1.6km(1 mile) sea at 21:45 on March 26, 2010. In terms of coastal researcher's point of view, it is meaningful to provide the sea condition of basic data necessary for search and rescue, more detailed predictions and inference data through the numerical simulations. Thus, in this study, we investigated the weather, wave, tide, tidal current, bottom soil conditions, and suspended sediment are investigated at the coast of Baekryeong-Daechung islands. And based on these data, the characteristics of sea conditions were analyzed. The tidal period at the time of incident corresponds between neap tide to mean tide. Until April 3-4 after March 26, the date of incident, the strongest velocity was progressed towards the spring tide. Thus, it was considered to be difficult to search and rescue operations. Also, because the ebb tide was in progress during 21:00 to 22:00, mass transport seems to be prevailed to the southeast. In particular, as the sudden turbulence due to the irregular topography existed was anticipated, we had carried out particle tracking experiment. From this experiment, depending on the situation of flow, the initial movement of the particles were directed to the southeast but it turned out moving towards the offshore based on the long term prediction. Through this result, it is considered that the scope of the search operation should be expanded towards the open sea.

• Journal of Biomedical Engineering Research
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• v.21 no.4
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• pp.391-401
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• 2000

Flow through compliant tubes with linear taper in wall thickness is numerically simulated by finite element analysis. For verification of the numerical method, flow through a compliant stenotic vessel is simulated and the results are compared to the existing experimental data. Steady two-dimensional flow in a collapsible channel with initial tension is also simulated and the results are compared with numerical solutions from the literature. Computational results show that as cross-sectional area decreases with the reduction in downstream pressure, flow rate increases and reaches the maximum when the speed index (mean velocity divided by wave speed) is near the unity at the point of minimum cross-section area, indicating the flow limitation or choking (flow speed equals wave speed) in one-dimensional studies. for further reductions in downstream pressure, flow rate decreases. The flow limitation or choking consist of the main reasons of waterfall effect which occurs in the airways, capillaries of lung, and other veins. Cross-sectional narrowing is significant but localized. When the ratio of downstream-to-upstream wall thickness is 2, the area throat is located near the downstream end. As this ratio is increased to 3, the constriction moves to the upstream end of the tube.

• Journal of The Korean Society of Emergency Medicine
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• v.28 no.5
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• pp.457-466
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• 2017

Purpose: Traumatic brain injury (TBI) is a significant cause of morbidity and mortality worldwide. Severity of the initial insult is one of the most significant factors affecting outcome following TBI. In order to investigate the mechanisms of cellular injury and develop novel therapeutic strategies for TBI, we designed a standardized animal TBI model and evaluated histological and functional outcomes according to the degree of impact severity. Methods: Male adult C57Bl/6 mice underwent controlled cortical impact (CCI) at varying depths of deflection (1.0-2.0 mm). We performed hematoxylin and eosin staining at 7 days after recovery from TBI. Neurobehavioral characterization after TBI was analyzed by the Barnes maze test, passive avoidance test, open field test, rotarod test, tail suspension test, and light/dark test. Results: We observed a graded injury response according to the degree of deflection depths tested (diameter, 3 mm; velocity, 3 m/s; and duration, 500 ms) compared to sham controls. In the Barnes maze test, the severe TBI (2 mm depth) group showed reduced spatial memory as compared with the sham and mild TBI (1 mm depth) groups at 7 days after TBI. There was a significant difference in the results of the open field test and light/dark test among the three groups. Conclusion: Our findings demonstrate that the graded injury responses following TBI resulted in differential histopathological and behavioral outcomes in a mouse experimental CCI model. Thus, a model of CCI with histologic/behavioral outcome analysis may offer a reliable and convenient design for preclinical TBI research involving mice.