• Korean Journal of Animal Reproduction
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• v.27 no.4
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• pp.317-324
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• 2003

The present study were performed to analysis the hematocrit and the red blood cells content into the blood plasma of the transgenic pigs harboring recombinent human erythropoietin gene (rhEPO). Mouse whey acidic protein (mWAP) linked to rhEPO gene was microinjected into pronuclei of porcine one-cell zygotes. After delivered of offspring, PCR analyses identified one mWAP-rhEPO transgenic founder offspring(F$_{0}$). The first generation of transgenic pig (F$_{0}$) harboring mWAP-hEPO appeared to be a male, and the second generation (F$_1$) pigs were made by natural mating of F$_{0}$ with domestic swine, and male and female transgenic pigs (F$_1$) were identified by PCR. The blood samples from transgenic and normal pigs were collected for 50 days during lactation and were counted the red blood cell (RBC) numbers and Hematocrit (HCT) content into the blood. The transgenic pigs expressing rhEPO in their blood gave rise to higher RBC numbers and HCT contents than control animals. rhEPO was secreted both in the blood and milk of genetically engineered pigs harboring rhEPO gene. Therefore, this study provides a model regarding the production of transgenic pig carrying hEPO transgene for biomedical research.earch.

• Korean Journal of Animal Reproduction
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• v.27 no.4
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• pp.299-307
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• 2003

The effects of additions/deletions in glycosylated residues of recombinant human EPO (rhEPO) produced in CHO-K1 on their secretion were examined. hEPO cDNA was amplified from human liver mRNA and cloned into the pCR2.1 TOPO. Using overlapping-extension site-directed mutagenesis method, glycosylation sites at 24th, 38th, 83rd, and 126th were respectively or accumulatively removed by substituting its asparagine (or serine) with glutamine. To add novel glycosylation sites, 69 and 105th leucine was mutated to asparagine. Mutant and wild type rhEPO constructs were cloned into the pcDNA3 expression vector with CMV promoter and transfected into CHO cell line, CHO-K1, to produce mutant rhEPO mutant rhEPO proteins. Enzyme-linked immunosorbant assay (ELISA) and Western analysis with monoclonal anti-EPO antibody were performed using supernatants of the cultures showing transient and stable expressions respectively. Addition of novel glycosylation reduced rhEPO secretion dramatically while deletion mutants had little effect except some double deletion mutants ($\Delta$24/83 and $\Delta$38/83) and triple mutant ($\Delta$24/38/83). This fact suggests that not single but combination of changes in glycosyl groups affect secretion of rhEPO in cell culture, possibly via changes in their conformations.

• Journal of Animal Reproduction and Biotechnology
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• v.35 no.2
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• pp.163-170
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• 2020

Partheno Embryo's research is known to play a very important role in identifying the development of embryonic cells or analyzing the genetic mechanisms of embryonic development, but the information on apoptosis formed during the early stage of development on Partheno Embryo is very little. Therefore, this study analyzed whether the embryonic cell death of unit embryos can be inhibited by adding Scriptaid, one of HDACi, which plays a role in demethylation of histone proteins as a method of regulating the cell cycle in the early embryo development of Partheno Embryo. As a result, the differentiation rate was higher in the group that added Scriptaid and FBS, but the cellular development was higher in the group that added pregnant serum to Scriptaid. As a result of analyzing the expression of the gene through IF and PCR, the group with the addition of gestational serum increased the expression of BCL2 and PCNA, which affects the anti-Casp3 action in cell survival. In addition, it is interpreted that treatment of Scriptaid for 16 hours, rather than 24 h treatment lowers the expression of Casp-3, a representative factor of apoptosis, and also increases embryonic development, thus affecting early embryo development. Therefore, it is concluded that the 16-hour treatment of Scriptaid and the use of gestational serum will inhibit cell death in the early embryonic development and increase the development rate of the embryo.

• Korean Journal of Animal Reproduction
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• v.11 no.3
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• pp.189-195
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• 1987

This study was conducted in order to obtain the information of the histological changes in each of six segments of the epididymal ducts in Korean native goats. Thirty-two Korean native male goats were examined, dividing into seven groups, at 4 weeks intervals from 8 to 32 weeks of age. The results obtained were as follows. 1. The epididymal ducts showed histologically an abrupt growth at the age of 16 weeks being followed by almost full maturation at the age of 24 weeks. Diameter of the cauda was steadily larger than that of the caput and corpus of the epididymal ducts. 2. Spermatozoa in the lumen of epididymal ducts were first observable at the age of 16 weeks, thereafter showing sparse in the lumen of caput, whereas most dense in the lumen of cauda in the density of spermatozoa. 3. Ducts in the caput and corpus were lined by ciliated columnar epithelium until the age of 12 weeks, and later by pseudostratified ciliated columnar epithelium which was composed of ciliated columnar cells, clear cells and basal cells. Ducts of cauda epididymis were lined by simple ciliated columnar epithelia until 12 weeks of age and later by simple or pseudostratified ciliated columnar epithelium, and two types of ducts (small ducts with high epithelium and large ducts with lower epithelium) were noted. Nucleus of the epithelial cells in the caput were located in the base of cells but in the corpus and cauda, those were located in the mid part of cells. cilia were most developed in the epithelia of the corpus.

• The KIPS Transactions:PartC
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• v.11C no.5
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• pp.697-702
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• 2004

As for the digital content, a reproduction is easy and manuscript is identical with original copy. Because of these characteristics, there are difficulties on prevention of an illegal reproduction and an illegal currency. In recent days various digital content service systems based on a web are commercialized. An appropriate copyright protection technology is required so that these systems develop as a profit model. Generally we use encrypted digital content transmission method for the copyright protection on a web base system. At the time of this, it is increased sire of encrypted digital content. As for this, it be increased time required on an execution process. Therefore, a design of the system that considered a execution time and a security is required. In this study, we designed the digital content transmission system that considered execution time and a security through a partial encryption based on a digital content copyright management technique. Also we evaluated performance of a proposed system through analysis.

• The Journal of the Acoustical Society of Korea
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• v.20 no.8
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• pp.3-11
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• 2001

This paper describes an implementation of inverse filter using SVD in order to recover the input in multi-channel system. The matrix formulation in SISO system is extended to MIMO system. In time and frequency domain we investigates the inversion of minimum phase system and non-minimum phase system. To execute an effective inversion of non-minimum phase system, SVD is introduced. First of all we computes singular values of system matrix and then investigates the phase property of system. In case of overall system is non-minimum phase, system matrix has one (or more) very small singular value (s). The very small singular value (s) carries information about phase properties of system. Using this property, approximate inverse filter of overall system is founded. The numerical simulation shows potentials in use of the inverse filter.

• Reproductive and Developmental Biology
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• v.40 no.2
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• pp.15-22
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• 2016

As a one of unsaturated fatty acid, polyunsaturated fatty acids (PUFAs) have multiple actions: as precursor of prostaglandins (PGs), steroid hormone synthesis and energy production in animal reproduction. PUFAs, which include omega-3 (n-3) and omega-6 (n-6), are derived from the diet and changed by diet, species, breed and season. The plasma membrane of spermatozoa in mammals contain various PUFAs. These composition of PUFAs regulate the membrane fluidity and cause lipid peroxidation via generation of reactive oxygen species (ROS). Induced lipid peroxidation by ROS decreased viability and motility of spermatozoa, and it is reduced by addition of antioxidant and low concentration of PUFAs. Because oocytes of animal have a high lipid components, process of oocyte maturation and embryo development are influenced by PUFAs. In in vitro study, oocyte maturation, embryo development, intracellular cAMP and MAPK activity were increased by treatment of n-3 ${\alpha}$-linolenic acid (ALA) during maturation, whereas n-6 linoleic acid (LA) negatively influenced. Also, inhibition of fatty acid metabolism in oocyte influenced blastocyst formation of cattle. PGs are synthesized from PUFAs and various PUFAs influence PGs via regulation of PG-endoperoxide synthase (PTGS). Steroid hormone synthesis from cholesterol is regulated by expression of steroid acute regulator (StAR) protein and mRNA. Exogenous n-3 and n-6 PUFAs altered sex hormone in animal through stimulate or inhibit StAR activity. Because PUFAs altered PG and steroid hormone synthesis, follicular development was influenced by PUFAs. This effect of unsaturated fatty acid could provide information for improvement of reproductive ability in animals.

• Journal of Internet Computing and Services
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• v.7 no.5
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• pp.95-108
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• 2006

The digital contents expand into software source code and maintenance of technology and IPR about source code have a very important meaning to international competition, The recognition about software security is very low specially among these Intellectual Property Rights. On occurring disputation property, we have to prove the fact, there is a problem to discriminate the original source code, Also, it is hard to accurate decision that is correct to complexity and the lack of read and understand ability even if software is reproduced. In this paper, we don't enforce distinction about software reproduction by one individual code unit. And we developed digital license prototype of XML that can distinguish reproduction based on structural conformability of whole source codes. Software has Context Free Grammar in structure and presents BNF notation type, it is apt to present hierarchical structure. Then, we can express architecture of software source code by hierarchical structure to discriminate structural conformability. In this paper, we make a study of the digital licence prototype for discriminate the original source code. Reserved words of software source code by parsing express to XML file that have hierarchical structure. Then, we can express architecture of software source code by tree structure form instead of complex source code.

• Journal of the Korean Graphic Arts Communication Society
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• v.20 no.2
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• pp.31-43
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• 2002

Screen printing is a stencil process whereby ink is transferred to the substrate through a stencil supported by a fine fabric mesh. Therefore screen had a tendency to distort and swell, as ink was deposited between the fibers, and were difficult to clean. The tow importance of stencil parameters that affect print quality are stencil thread diameter and the fabric thickness because of their influence on both ink deposit and print definition. Since screen printing inks can be formulated to adhere to almost any surface, and the printing process itself can be handled almost any substrate in a wide variety of shape, screen printing is a very versatile process. The small size pronting is reproduced image used screen printing because the surface of substrates is not suited at screen printing method. In screen printing, the need of high definition printing is gradually increasing according to developing special inks. A conventional haftone, so called AM screening, is simple and easy to implement, but the haftone dot patterns by using this method are not free for the moire fringe. This paper is used densitometry and image analysis to investigate relation with printing according to screen mesh, opening size and resolution of copy in image reproduction used FM screen. We had the good result of dot gain and tone reproduction on the screen printing of high definition using FM screen.

• Journal of Animal Reproduction and Biotechnology
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• v.35 no.1
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• pp.65-72
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• 2020

Fish ovarian germline stem cells (OGSCs) that have the abilities to self-renew and differentiate into functional gametes can be used in various researches and applications. A main issue to be solved for effective utilization of fish OGSCs is the development of their stable in vitro culture condition, but only few researches about fish OGSC culture have been reported so far. In this study, in order to find the clues to develop the culture condition for OGSCs from Japanese medaka (Oryzias latipes), we tried to establish somatic cell lines as a candidate for the feeder cells and evaluated its supporting effects on the culture of ovarian cell populations from O. latipes. As the results, the somatic cell lines could be established only from the embryonic tissues among three tissues derived from embryos, fins and ovaries. Three embryonic cell lines were tested as a feeder cell for the culture of ovarian cell population and all three cell lines induced cell aggregation formation of the cultured ovarian cells whereas the feeder-free condition did not. Furthermore, a significant cellular proliferation was observed in the ovarian cells cultured on two of three cell lines. As a trial to increase the capacity of the cell lines as a feeder cell that supports the proliferation of the cultured ovarian cells, we subsequently established a stable line that expresses the foreign O. latipes fibroblast growth factor 2 (FGF2) from an embryonic cell line and evaluated its effectiveness as a feeder cell. The ovarian cells cultured on FGF2 expressing feeder cells still formed cell aggregates but did not show a significant increase in cellular proliferation compared to those cultured on non-transformed feeder cells. The results from this study will provide the fundamental information for in vitro culture of medaka OGSCs.