• Biomedical Science Letters
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• v.11 no.4
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• pp.429-434
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• 2005

Apolipoprotein E (apoE) restriction isotyping used oligonucleotides to amplify apoE gene sequences containing amino acid positions 112 and 158. The amplification products were digested with HhaI and subjected to electrophoresis on $4\%$ agarose gel. Each of the isoforms was distinguished by a unique combination of HhaI fragment sizes that enabled unambiguous typing of all homozygotic and heterozygotic combinations. HhaI cleaves at GCGC encoding 112arg (E4) and 158arg (E3, E4), but does not cut at GTGC encoding 112cys (E2, E3) and] 58cys (E2). DNA was isolated from 72 study participants and apoE genotypes were determined utilizing the polymerase chain reaction and restriction isotyping. In the entire group of subjects, $38 (52.8\%)$ had apo E4/4 or E3/4 (Group E4), $28(38.9\%)$ had the apo E3/3 genotype (Group E3) and $6(8.3\%)$ had apo E2/2 or E2/3 (Group E2). This genotypic information may help to identify individuals at increased risk for several diseases.

• The Plant Pathology Journal
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• v.30 no.3
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• pp.310-315
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• 2014

Tomato yellow leaf curl virus (TYLCV), a member of the genus Begomovirus, is responsible for one of the most devastating viral diseases in tomato-growing countries and is becoming a serious problem in many subtropical and tropical countries. The climate in Korea is getting warmer and developing subtropical features in response to global warming. These changes are being accompanied by TYLCV, which is now becoming a large problem in the Korean tomato industry. The most effective way to reduce damage caused by TYLCV is to breed resistant varieties of tomatoes. To accomplish this, it is necessary to establish a simple inoculation technique for the efficient evaluation of resistance to TYLCV. Here, we present the rolling circle amplification (RCA) method, which employs a bacteriophage using phi-29 DNA polymerase for construction of infectious TYLCV clones. The RCA method is simple, does not require sequence information for cloning, and is less expensive and time consuming than conventional PCR based-methods. Furthermore, RCA-based construction of an infectious clone can be very useful to other emerging and unknown geminiviruses in Korea.

• Proceedings of the Korean Geotechical Society Conference
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• 2008.03a
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• pp.563-574
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• 2008

Most of earthquake-induced geotechnical hazards have been caused by the site effects relating to the amplification of ground motion, which are strongly influenced by the local geologic conditions such as soil thickness or bedrock depth and soil stiffness. In this study, an integrated GIS-based information system for geotechnical data, called geotechnical information system (GTIS), was constructed to establish a regional counterplan against earthquake-induced hazards at an urban area, Daejeon, which is represented as a hub of research and development in Korea. To build the GTIS for the area of interesting, pre-existing geotechnical data collections were performed across the extended area including the study area and a walk-over site survey was additionally carried out to acquire surface geo-knowledge data. For practical application of the GTIS used to estimate the site effects at the area of interesting, seismic microzoning map of the characteristic site period was created and presented as regional synthetic strategy for earthquake-induced hazards prediction. In addition, seismic zonation for site classification according to the spatial distribution of the site period was also performed to determine the site amplification coefficients for seismic design and seismic performance evaluation at any site in the study area. Based on the case study on seismic zonations at Daejeon, it was verified that the GIS-based GTIS was very useful for the regional prediction of seismic hazards and also the decision support for seismic hazard mitigation.

• Analytical Science and Technology
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• v.22 no.6
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• pp.498-503
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• 2009

Lung cancer is the main cancer on the world today, due to the high case fatality. Lung cancer can devide into two major types, such as small-cell lung cancer (SCLC) and non-small-cell lung cancer (NSCLC). Mutations in the epidermal growth factor receptor (EGFR) have been described in patients with advanced NSCLC. Mutations in the EGFR are associated with clinical and radiographic responses to EGFR tyrosine kinase inhibitors gefitinib and erlotinib. Thus, the detection of EGFR mutation can offer an effective information in clinical decision-making. In this study, We developed very simple, cheep and rapid mutation detection system by chip-based isothermal amplification method. The method described here has shown the advantages of rapid amplification, high sensitivity, and specificity. Also, it will be useful for rapid and reliable clinical diagnosis of EGFR mutation.

• Proceedings of the IEEK Conference
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• 1998.10a
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• pp.319-322
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• 1998

A 20 GHz 2-stage MMIC (Monolithic Microwave Integrated Circuits) LNA(Low Noise Amplifiers) has been designed. The pHEMT with gate length of 1.15 um has been used to provide ultra low noise and high gain amplification. Series and Shunt feedback circuits were interted to ensured high stability over frequency range of DC to 60 GHz. The size of designed MMIC LNA is 2285um x 2000um(4.57mm2). The simulated noise figure of MMIC LNA is less than 1.7 dB over frequency range of 20 GHz to 21 GHz.

• Journal of Communications and Networks
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• v.1 no.2
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• pp.81-85
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• 1999

The regrowth of OQPSK power spectral sidelobes from AM/AM and AM/PM amplifier nonlinearity is analyzed. The time-domain expression for amplifier output shows how spectral re-growth will depend on the cubic coefficient of the Taylor's series of the amplifier nonlinearity as well as input amplitude ripple. Closed form spectrum calculations show that the spectral sidelobes produced by AM/PM take the same form as those produced by AM/AM. The rate of growth of AM/PM sidelobes is, however, not as great as for AM/AM.

• Proceedings of the IEEK Conference
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• 2003.11a
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• pp.355-358
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• 2003

Basically, the mis-convergence, which is inevitable in CRT Projection TV. is the degree of deviation of red and blue from green beam. The cause of mis-convergence is the change of magnetic field and electrical characteristic in deflection circuits and convergence amplification circuit. A new and easily implementable mis-convergence compensation algorithm is presented in this paper. The proposed algorithm does not needs any compensation devices. It uses only TV OSD and a remote controller and anyone who wants to compensate can easily correct the mismatch. Through real compensation experiments, it is found that the proposed algorithm is useful and effective one.

• Proceedings of the IEEK Conference
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• 1999.06a
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• pp.1149-1152
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• 1999

A gain controllable precision full-wave rectifier for the measurements of small-signal voltage is presented. It consists of gain controllable inverter superdiode and noninverter superdiode. The results of simulation with PSpice and experiment on breadboard show that the proposed rectifier has the characteristic of precise rectification and amplification for small signal voltage.

• Proceedings of the IEEK Conference
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• 1999.11a
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• pp.1163-1166
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• 1999

In this paper, A CMOS power amplifier for PCS is designed with 0.65-$\mu\textrm{m}$ CMOS technology. Differential cascode structure is used which has good reverse isolation and wide voltage swing. This amplifier circuits consist of three stages which are power amplification stage, driver stage and power control stage. We obtain output power of 30 ㏈m, IMD3 of -31㏈c and efficiency of 30 % at input power of 4 ㏈m.

• Proceedings of the IEEK Conference
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• 2006.06a
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• pp.815-816
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• 2006

In this paper, an EMG (Electromyography) sensor for utilizing an EMGl-based HCI are described. The EMG sensor is a dry type and has high gain (1000-10000). Therefore, this sensor can be properly applied to HCI devices using EMG signals without additional amplification circuit.