• 생명과학회지
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• 제30권9호
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• pp.749-762
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• 2020

돼지인플루엔자는 동물에서 사람에게 감염할 수 있는 인수공통전염병이다. 우리는 2019년 한국 돼지농장에서 호흡기 증상을 보이는 돼지에서 3주의 H1N2형 인플루엔자바이러스를 분리하였다. 유전자 분석결과, 이들 바이러스의 8개 유전자 중 PA 및 NP 유전자는 2009 대유행 H1N1 인플루엔자 유래였고, 나머지 유전자는 돼지에 유행하는 H3N2 및 H1N2 인플루엔자 유래 유전자를 가진 재조합 바이러스 이었다. 분리된 H1N2 바이러스를 마우스에 접종한 결과, 마우스는 17% 정도 체중이 감소하였고, 염증 세포들이 침윤한 간질성 폐렴 증상을 보였다.

• Journal of Microbiology and Biotechnology
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• 제17권5호
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• pp.868-872
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• 2007

H1N2 influenza viruses are circulating in pigs worldwide and cause considerable economic losses to the pig industry. We genetically analyzed the genes of our isolates from Korean pigs, and compared the antigenicity of our isolates with swine H1N2 viruses isolated from pigs in the U.S.A. In addition, we serologically surveyed the infection rate of swine H1N2 viruses in pigs. We found that H1N2 isolates from Korean pigs are genetically more related to swine H1N2 viruses isolated from pigs in the U.S.A. than those in European countries. When antigenicity was compared, our isolates were weakly reacted to antibodies against swine H1N2 viruses isolated from pigs in the U.S.A. The serological surveillance using sera from pigs in Korea showed that about 46% was positive for H1N2 viruses. Our results suggest that swine H1N2 viruses are widespread in Korean pigs, and the development of a vaccine against H1N2 viruses may help to control their infection in pigs.

• Journal of Veterinary Science
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• 제22권2호
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• pp.21.1-21.6
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• 2021

In this study, we describe the isolation and characterization of previously unreported Y280-lineage H9N2 viruses from two live bird markets in Korea in June 2020. Genetic analysis revealed that they were distinct from previous H9N2 viruses circulating in Korea and had highest homology to A/chicken/Shandong/1844/2019(H9N2) viruses. Their genetic constellation showed they belonged to genotype S, which is the predominant genotype in China since 2010, where genotype S viruses have infected humans and acted as internal gene donors to H5 and H7 zoonotic influenza viruses. Active surveillance and control measures need to be enhanced to protect the poultry industry and public health.

• 생명과학회지
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• 제28권8호
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• pp.962-968
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• 2018

A형 인플루엔자바이러스는 야생조류에 존재하며, 사람, 돼지, 가금 및 다른 포유류등 다양한 숙주를 감염한다. 본 연구에서는, 2016년 한국 서쪽의 철새도래지에서 채취한 철새 분변에서 재조합된 새로운 H7N7 조류인플루엔자를 분리하였으며 이 바이러스의 8개 유전자를 분석하였다. 분리된 A/waterfowl/Korea/S017/2016(H7N7) 바이러스의 유전자 분석 결과 이 바이러스는 야생조류 및 가금 오리에서 유래한 조류인플루엔자로 구성된 재조합된 유전자를 가지고 있었다. 계통 분석결과 이 바이러스는 유럽과 아시아계에 속하였다. 조류인플루엔자 바이러스가 계속 진화를 하고 H7 형 조류인플루엔자는 고 병원성 조류인플루엔자로 변하여 사람과 동물에게 커다란 위협이 될 수 있기에 계속 된 역학조사가 필요하다.

• 한국환경보건학회지
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• 제39권3호
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• pp.230-238
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• 2013

Objectives: For our survey of the incidence of influenza viruses among respiratory viral infections in Seoul, we evaluated their prevalence among infectious acute respiratory viral patients in Seoul from 2010 to 2012 through regular surveillance. Methods: For influenza virus detection, we conducted real-time PCR analyses on 2,544 throat specimens collected from patients with respiratory viral infections in Seoul between 2010 and 2012. They were collected and then tested for the presence of influenza viruses through reverse transcription (RT) - polymerase chain reaction (PCR). Results: 19.1% (486/2,544) of the throat specimens were determined to be positive for influenza viruses. The incidences of influenza viral infection in the case of respiratory viral infections through regular surveillance in Seoul were 23.0% (212/923) in 2010, 6.4% (47/738) in 2011, and 25.7% (227/883) in 2012, and 10.8% (275/2,544) of type A, and 8.3% (211/2,544) type B influenza viruses. In addition, the greatest prevalence was in the 20-49 age group (51.6% ), which shows that influenza viruses constituted a major causative agent of acute respiratory viral infections. Conclusions: The distributions of influenza viruses and the epidemiologic patterns of the viral pathogen in acute respiratory viral infectious patients may provide potentially effective data for epidemiological studies in Seoul, Korea.

• Journal of Microbiology and Biotechnology
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• 제20권1호
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• pp.63-70
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• 2010

Novel influenza A (H1N1) is a newly emerged flu virus that was first detected in April 2009. Unlike the avian influenza (H5N1), this virus has been known to be able to spread from human to human directly. Although it is uncertain how severe this novel H1N1 virus will be in terms of human illness, the illness may be more widespread because most people will not have immunity to it. In this study, we compared the codon usage bias between the novel H1N1 influenza A viruses and other viruses such as H1N1 and H5N1 subtypes to investigate the genomic patterns of novel influenza A (H1N1). Totally, 1,675 nucleotide sequences of the hemagglutinin (HA) and neuraminidase (NA) genes of influenza A virus, including H1N1 and H5N1 subtypes occurring from 2004 to 2009, were used. As a result, we found that the novel H1N1 influenza A viruses showed the most close correlations with the swine-origin H1N1 subtypes than other H1N1 viruses, in the result from not only the analysis of nucleotide compositions, but also the phylogenetic analysis. Although the genetic sequences of novel H1N1 subtypes were not exactly the same as the other H1N1 subtypes, the HA and NA genes of novel H1N1s showed very similar codon usage patterns with other H1N1 subtypes, especially with the swine-origin H1N1 influenza A viruses. Our findings strongly suggested that those novel H1N1 viruses seemed to be originated from the swine-host H1N1 viruses in terms of the codon usage patterns.

• Journal of Microbiology
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• 제43권4호
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• pp.366-369
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• 2005

Avian influenza viruses playa crucial role i,n the creation of human pandemic viruses. In this study, we have demonstrated that both human and avian influenza receptors exist in cells in the respiratory and intestinal tracts of chickens. We have also determined that primarily cultured chicken lung cells can support the replication of both avian and human influenza viruses.

• 생명과학회지
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• 제17권3호통권83호
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• pp.340-344
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• 2007

조류인플루엔자바이러스는 사람 및 동물에 상당한 위협을 가하고 있다. 이 연구는 알칼리성 소독용액이 H5N1, H3N2, H6N1, H9N2형 조류인플루엔자바이러스를 불활성화시킬 수 있는지를 조사했다. 알칼리성 소독용액을 생리식염수에 100배 희석한 경우 조류인플루엔자가 MDCK 세포에서 증식하는 것을 완전히 억압하였다. 형광항체법을 이용한 실험에서도 알칼리성소독액을 처리한 H9N2 조류인플루엔자바이러스는 MDCK세포에서 증식이 억제되었고, 알칼리성 소독액을 처리하지 않은 H9N2조류인플루엔자바이러스는 증식이 억제되지 않았다. 이 결과는 알칼리성 소독액은 고병원성 H5N1을 포함을 조류인플루엔자바이러스를 방역하는데 도움이 될 수 있음을 시사한다.

• 대한수의학회지
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• 제53권4호
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• pp.193-197
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• 2013

Avian influenza viruses (AIV) have been isolated from a wide range of domestic and wild birds. Wild birds, predominantly ducks, geese and gulls form the reservoir of AIV in nature. The viruses in wild bird populations are a potential source of widespread infections in poultry. Active surveillance for AIV infection provides information regarding AIV distribution, and global AIV surveillance can play a key role in the early recognition of highly pathogenic avian influenza (HPAI). Since 2003 in Korea, there have been four H5N1 HPAI outbreaks caused by clade 2.5, 2.2 and 2.3.2. Therefore, improvement of AIV surveillance strategy is required to detect HPAI viruses effectively. This article deals with the major events establishing the role of wild birds in the natural history of influenza in Korea. We highlighted the need for continuous surveillance in wild birds and characterization of these viruses to understand AIV epidemiology and host ecology in Korea.

• 생명과학회지
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• 제33권8호
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• pp.605-611
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• 2023

최근에 재조합 H7Nx 인플루엔자 바이러스가 산발적으로 인체 감염 사례가 보고되고 있으며 이러한 바이러스는 조류 종으로부터 지속적으로 분리되고있다. 본 연구에서는 조류에서 유래된 H7N1 인플루엔자 바이러스를 분리하여 A/wild bird/South Korea/sw-anu/2023로 명명하였고, 전장유전체 분석과 분자적 특성을 분석하였다. 계통발생학적 분석 결과 A/wild bird/South Korea/sw-anu/2023는 유라시아 혈통에 속하는 H7N1 인플루엔자 바이러스로 확인되었다. A/wild bird/South Korea/sw-anu/2023 바이러스의 polymerase basic 1(PB)2, PB1, polymerase acidic (PA), nucleoprotein (NP) 유전자는 야생 조류에서 분리되었던 조류 인플루엔자 바이러스유전자와 밀접한 관련이 있는 것으로 밝혀졌으며, hemagglutinin (HA), neuraminidase (NA), matrix (M), nonstructural (NS) 유전자는 집오리에서 분리되었던 조류 인플루엔자 바이러스와 유사하였다. 이러한 결과는 동아시아-호주 이동 경로를 따라 이동하는 야생 조류들 사이에서 새롭게 유전자가 재배열된 재조합 H7N1 조류 인플루엔자 바이러스가 순환되고 있음을 시사하고 있다. 따라서, H7Nx 인플루엔자 바이러스는 전 세계적으로 순환하며, 돌연변이된 H7N1 조류 인플루엔자 바이러스는 인간을 감염시킬 수 있으므로 야생 조류 및 가금류에서 H7N1 조류 인플루엔자 바이러스의 지속적인 감시가 필요할 것이다.