• Journal of Acupuncture Research
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• 제18권5호
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• pp.204-212
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• 2001

Objective : Septic arthritis is a disease of when bacterial infection causes inflammatory reactions to occur in the body's joints. Following damage, stiffness, and disformity may occur in the joints. Initially antibiotic treatment is used. Later treatment is the preservation and rehabilitation of the joints. Here we report a case of sequelae of septic arthritis, which is treated and managed by ways of oriental medicine. Methods : We considered that symptoms like fever, arthralgia and ankylosis were similar to Yeol-bi(熱痺) and the patient's constitution was Soyang-in(少陽人). So Yangguksanhwa-tang(凉膈散化湯) was used as medication, and acupuncture, TENS, rehabilitation treatment was carried out. Also Kinesio & Cross(Geok-ja(격자)) taping therapy was done after the contact test. Results : After 4-7 days from the end of antibiotic treatment, the patient regained movement in the joints. After 3 weeks, the patient was able to support his own weight, and one week later joints were restored to nearly normal range. Therefore sequelae of Septic arthritis is thought to be treated earlier when the above treatment is used, compared to western treatment, which mainly consist of antibiotic treatment. Conclusion : This case was managed by herbal medicine, acupuncture, TENS, rehabilitation treatment, and Kinesio & Cross(Geok-ja) taping therapy on sequelae of Septic arthritis. Earlier improvement was gained comparatively to western treatment, which mainly consist of antibiotic treatment.

• Journal of Pharmacopuncture
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• 제26권3호
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• pp.257-264
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• 2023

Objectives: The study was designed to evaluate anti-arthritic activity of Ajmodadi Churna (AC) and its effect on Complete freund's adjuvant (CFA)-induced arthritis in Wistar rats. Methods: Arthritis was induced by injecting 0.2 mL CFA into sub plantar surface of left hind paw. Test sample AC-1 and AC-2, 200 and 400 mg/kg, respectively was given to the animals for 21 consecutive days. The increase in swelling was observed after induction of arthritis. The paw edema was measured on 0, 3, 7, 14 and 21 day using Vernier caliper after the induction of arthritis. The collected blood samples further used for the estimation of red blood cells (RBC), white blood cells (WBC), erythrocytes sedimentation rate (ESR), and hemoglobin (Hb), using hematology analyzer. Serum concentration of IL-6 and TNF-α were also measured using rat ELISA kits. Results: Results showed that a significant reduction in paw edema was observed in AC-2 treated rats. The paw edema was restored on day 21 was 4.48 mm for AC-2, which is near to the control group. The arthritis score in treated rats was found to be considerably lower than in the control group i.e. 0.83 for AC-2 and 1.50 for AC-1. A decrease in levels of RBC and hemoglobin were observed in arthritic rats. Inflammation was significantly reduced and serum levels of IL-6 and TNF-α were lowered after treatment with the test drug. Conclusion: It can be concluded from the study that AC possess significant anti-arthritic activity. Furthermore, this condition was linked to a reduction in abnormal humoral immune responses.

• Herbal Formula Science
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• 제16권1호
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• pp.79-94
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• 2008

Although inflammatory mediators such as nitric oxide(NO) and pro-inflammatory cytokines are involved in host defense mechanism, these overproduction contributes to the pathogenesis of several diseases such as otitis media, hearing loss, periodontitis, bacterial sepsis, rheumatoid arthritis, chronic inflammation and autoimmune diseases. We investigate the anti-inflammatory effects of water extract from Ji-Pae-San(JPSWE) fomulated with Angelica dahurica plus Fritillaria Verticillata, Angelica dahurica(ADWE), and Fritillaria Verticillata(FUVE) in vitro and in vivo. Each extract inhibited the production of inflammatory mediators(NO, $IL-1{\beta}$, IL-6, $TNF-{\alpha}$, and prostaglandin $E_2$) and the expression of inducible NO synthase(iNOS) and cyclooxygenase-2(COX-2) in lipopolysaccharide(LPS)-stimulated RAW 264.7 macrophages in a dose-dependent manner. These inhibitory effects were synergistically increased by their combination. JPSWE also inhibited $TNF-{\alpha}$, $IL-1{\beta}$, IL-6. and $PGE_2$ production as well as COX activity in LPS-stimulated mice. Moreover, JPSWE significantly suppressed death by LPS-septic shock in mice(survival rate: 100%). These results suggest that Ji-Pae-San may be useful for therapeutic drugs against inflammatory immune diseases, probably by suppressing the production of inflammatory mediators.

• Molecular & Cellular Toxicology
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• 제1권2호
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• pp.124-129
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• 2005

Pro-inflammatory cytokines, such as tumor necrosis factor $(TNF)-{\alpha}$, interleukin-12 (IL-12) and interleukin $(IL-1)-{\beta}$, play a key role in causing inflammatory diseases, which are rheumatoid arthritis, Crohn's disease and sepsis. Accumulating evidences suggest that low level laser irradiation (LLLI) may have an anti-inflammatory action. However, there are few data regarding down regulation of Th1 immune response by using the diod typed laser emitting device for human patients. As a fundamental step in order to address this issue, we investigated immunological impact of the low level laser irradiation (10 mw laser diode with a wavelength of 630 nm) on expression of pro-inflammatory cytokines in murine immunocytes (splenocytes and peritoneal macrophages) in vitro. The LLLI on lipopolysaccharide (LPS 100 ng/ml)-stimulated murine splenocytes and macrophages, clearly down regulated mRNA expression of $TNF-{\alpha}$ and IL-12 in dose-dependent manner. In addition, LLLI significantly inhibits the NO production in the LPS-stimulated murine macrophages. This data suggests that LLLI (wavelength of 630 nm) may exert an anti-inflammatory action via modulation of pro-inflammatory cytokine and NO production pathway.

• Biomolecules & Therapeutics
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• 제2권1호
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• pp.71-76
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• 1994

Anti-inflammatory and analgesic activities and skin irritation of piroxicam patch were investigated. Piroxicam patch increased the pain threshold in rat hind paw inflamed by carrageenan and inhibited writhing induced by acetic acid in mice. Piroxicam patch also inhibited the carrageenan-induced edema in rat hind paw as well as the increased vascular permeability induced by histamine in rats. In adjuvant arthritis of rats, piroxicam patch showed anti-inflammatory effects. Skin irritation of piroxicam patch was tested in Newzealand White rabbits and evaluated by Primary Irritation Index of Draize. The results from skin irritation test showed that piroxicam patch seemed practically non-irritating. The result from the present study indicates that piroxicam may be useful without serious side effects as anti-inflammatory analgesics in this patch form.

• The Korea Journal of Herbology
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• 제25권4호
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• pp.137-143
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• 2010

Objectives : The present study was conducted to examine the effect of Cudraniae Tricuspidatae Radix(CTR), which is the radix part of Cudrania tricuspidata Bureau, on cytokine secretion from the joint cells and spleen cells of mice with arthritis induced by collagen and verify its efficacy against rheumatoid arthritis. Methods : Three kinds of extract were prepared from CTR through extraction with hot-water and ethanol. The levels of cytokine secreted from the cells were measured, after the mice knee joint cells were cultured with each extract. Results : The three kinds of extracts from CTR decreased the growth rate and levels of inflammatory cytokines such as IL-$1{\beta}$, IL-6, and TNF-${\alpha}$ from knee joint cells of mice. All of the organic-soluble fraction, such as hexane, ethyl ether, ethyl acetate, butanol, and residual water-soluble fraction decreased the levels of IL-$1{\beta}$, TNF-${\alpha}$, IL-6, and IL-10. Conclusion : These results suggest that CTR had some effects on rheumatitis, with fat and water-solubles in organic solvent considered as the effective element.

• Journal of Korean Academy of Nursing
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• 제17권3호
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• pp.218-226
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• 1987

This descriptive-correlational study was undertaken in order to examine if there were relationships between family support, compliance and life satisfaction in chronic arthritis patients. The sampling method was a non-probability, purposive sampling technique. The participants of this study were 92 volunteer adults. 1) who have been diagnosed as having chronic arthritis and 2) who were at the medical out patient clinic of one university hospital in Seoul between April 27th to May 12th 1987. The instruments used for this study were the Family Support Scale developed by Kang Hyun Sook, a Compilance Scale developed by the researcher and the Life-Satisfaction Index-Z developed by Wood et.al. Analysis of data was done using t-test, ANOVA, Pearson Correlation Coefficient and Stepwise Multiple Regression Analysis. The results of study were as follows; Hypothesis I; “The higher the family support, the higher the compliance” was accepted. (r＝.2604, p＜.01) Hypothesis II; “The higher the compliance, the higher the life satisfaction,” was rejected. (r＝.1663, p＞0.5) Hypothesis III; “The higher the family support, the higher the life satisfaction” was accepted. (r＝.3914, p＜.01) In addition, by using Stepwise Multiple Regression Analysis it was determined that the main influencing factors on the life satisfaction were family support, the number of inflammatory joints, compliance and pain level. These 4 main variables made it possible to explain 30.8% of the variance in life satisfaction. In conclusion, this study revealed that family support is an important factor that can improve compliance and life satisfaction in chronic arthritis patients.

• Journal of Pharmaceutical Investigation
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• 제26권4호
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• pp.273-280
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• 1996

Chondroitin sulfate/gelatin microspheres containing dexamethasone 21-acetate were prepared by complex coacervation method and their release patterns were examined in vitro. Microspheres prepared with a small amount of crosslinking agent had smooth surface and few pores, but those with a large amount of crosslinking agent were more porous and less spherical. In vitro release patterns were varied by changing polymer/drug weight ratio and amount of crosslinking agent. The release rate of dexamethasone 21-acetate in the presence of collagenase was faster than that in the absence of collagenase. Anti-inflammatory effect of dexamethasone 21-acetate microspheres was more efficient than that of dexamethasone 21-acetate solution in carrageenan-induced arthritis in the rat. On the basis of the above results, we might expect the degradation and drug release rate of these microspheres to be regulated by the degree of crosslinking and the level of enzymes. In patients with severe rheumatoid arthritis who have high concentration of collagenase, more drug would be released from the microspheres. An intra-articular injection therapy of rheumatoid arthritis with desired release kinetics could be developed to enhance patient compliance and therapeutic index.

• Journal of Physiology & Pathology in Korean Medicine
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• 제20권5호
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• pp.1241-1244
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• 2006

This study was carried out to know the effect of Geopungjeseup-tang(GPJST) to on Rheumatoid Arthritis by using human fibroblast-like synoviocytes(hFLS). GPJST consists of Coisis Semen, Coccllus Trilobus DC., Lycopi Herba, Arisaematis Rhizoma. The author peformed several experimetal items : that is cytotoxicity of GPJST, mRNA expression of TNF-${\alpha}$, IL-1${\beta}$, ICAM-1, production of NO. The results were obtained as follows : The mRNA expression of pro-inflammatory cytokines TNF-${\alpha}$, IL-1${\beta}$, ICAM-1 were dose-dependently reduced in hFLS. The production of NO was reduced. Talking all these observations into account, GPJST is considered to be effective in treating rheumatoid arthritis. Therefore we have to survey continuously in looking for the effective substance and mechanism in the future.

• Biomolecules & Therapeutics
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• 제31권5호
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• pp.536-543
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• 2023

Phytoceramide (Pcer) is found mainly in plants and yeast. It can be neuroprotective and immunostimulatory on various cell types. In this study, the therapeutic effect of Pcer was explored using the carrageenan/kaolin (C/K)-induced arthritis rat model and fibroblast-like synoviocytes (FLS). Pcer treatment (1, 10, and 30 mg/kg/day) were given to the arthritic rats for 6 days after disease induction. Weight distribution ration (WDR), knee thickness, squeaking score, serum levels of proinflammatory mediators, and histological analysis were measured and performed to evaluate arthritic symptoms in the rat model. In interleukin (IL)-1β-stimulated FLS, proinflammatory mediators were measured after Pcer (1-30 µM) treatment. Arthritic symptoms in rats with Pcer treatment were significantly decreased at days 4 to 6 after C/K arthritis induction. Inflammation in the knee joints were also significantly decreased in rats with Pcer treatment. Furthermore, in IL-1β-stimulated FLS, the expressions of proinflammatory mediators were also inhibited by Pcer. As shown by the results, Pcer has anti-arthritic effects in the C/K rat model and in synovial cells, suggesting that Pcer has the potential to be a useful agent in arthritis treatment.