• Journal of Microbiology and Biotechnology
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• 제13권2호
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• pp.251-255
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• 2003

The bacterial compositions of seven hot-pepper sown soil were compared in this study. From the 624 isolates, 95 species and 49 genera were identified by fatty acid methyl ester analysis (FAME). The FAME results of seven soil showed two distinct clusters for aerobic and Gram-negative bacteria in the high productivity and monoculture soil samples. While Arthrobacter ($17\%$), Kocuria ($11\%$), Pseudomonas ($8\%$), and Bacillus ($8\%$) were predominant among bacteria which were cultured on heterotrophic (YG) agar medium, Pseudomonas ($56\%$), Stenotrophomonas ($16\%$), and Burkholderia ($8\%$) were predominant on crystal violet agar medium. Shannon Weaver indices (H) indicated that colonies obtained from heterotrophic agar medium (3.1) were found to be more diverse than those obtained from the crystal violet media (1.9). The results suggest that FAME analysis may be a potential indicator for of soil quality.

• Journal of Ecology and Environment
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• 제47권3호
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• pp.157-167
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• 2023

Background: The most hazardous wastewater sources in the northern part of the Middle Nile Delta, Egypt; receiving a massive amount of agricultural, industrial, and sewage drainage are Kitchener drain which is one of the tallest drainage systems, and Burullus Lake which represents the 2nd largest Egyptian coastal lake. Results: The current work is to determine the abundance and frequency of cyanophytes, chlorophytes, and bacillariophytes and the correlation between them and environmental abiotic components. Among sixty nine microalgal species, 19 species are belong Cyanophyta, 26 belong Chlorophyta and 24 belong Bacillariophyta. Genus Scenedesmus (Chlorophyta) was the most abundant in the study area (13 species), followed by Genus Oscillatoria (9 species) and Genus Navicula (7 species). Nostoc muscorum and Chlorella vulgaris were the most common and recorded in all sites (100% of the locations) under study. The application of the two-way indicator species analysis (TWINSPAN) and detrended correspondence analysis revealed agglomerating of 4 groups (communities) at 4th level of classification and reasonable segregation between these groups. Zinc, cadmium and lead were showed the highest levels (0.26±0.03, 0.26±0.06, and 0.17±0.01 ppm, respectively). Conclusions: The correlation analysis between water and community variables indicated a high negative correlation of total algae richness with nickel (r = -0.936, p < 0.01). Cyanophyta and Bacillariophyta were correlated negatively (r = -0.842, p < 0.01). However, Chlorophyta showed a negative richness with each of Ni and Pb (r = -0.965, -0.873, respectively) on one hand and a high positive correlation was revealed (r = 0.964) with all environmental variables on the other hand.

• 환경생물
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• 제28권1호
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• pp.1-7
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• 2010

Small wetlands in an upland matrix can support diverse vegetation composition that increase both local and regional species richness. In this study we characterize the full range of wetland vegetation in an upland forest landscape at Dumyeong-ri, Gijang-gun, Busan. This wetland index can be calculated with species data, or with community type data as performed. Classified community types were used to describe vegetation at three wetlands and adjacent areas. The communities contained 28 species of vascular plants and 28 species were identified four plant community types. The Pinus densiflora type was dominated by Pinus densiflora and contained only four species. None of the plots had high proportion of standing water. The Carpinus laxiflora type had high obligate upland species (OU) and facultative upland species (FU). The Rhododendron mucronulatum type grew in over half of the plots included Pinus densiflora and Alnus japonica. Some species bother swampy areas adjacent to site C. The Miscanthus sacchariflorus type consisted of seasonal wetlands. The three sites contained nine species with the strongest indicator species being Miscanthus sinensis var. purpurascens, Miscanthus sinensis, Echinochloa crus-galli, and Sagittaria aginashi. This type had the highest proportions of obligate wetland species. Plant species richness averaged 5.069. Shannon-Weaver index of diversity also varied among the community types (F=22.7, df=4, 115), with the types FU having significantly higher value (2.746) than the others (1.057 for type FW and 1.600 for type OU). Regional plans including all of the diverse types of wetland vegetation in upland forests will contribute substantially to the conservation of plant diversity.

• Journal of Microbiology and Biotechnology
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• 제26권4호
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• pp.648-658
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• 2016

Preservation of fresh algae plays an important role in algae seed subculture and aquaculture. The determination and examination of the changes of cell viability, composition, and bacterial species during storage would help to take suitable preservation methods to prolong the preservation time of fresh algae. Nostoc flagelliforme is a kind of edible cyanobacterium with important herbal and dietary values. This article investigated the changes of bacterial species and biochemical characteristics of fresh N. flagelliforme concentrate during natural storage. It was found that the viability of cells decreased along with the storage time. Fourteen bacteria strains in the algae concentrate were identified by PCR-DGGE and were grouped into four phyla, including Cyanobacteria, Firmicutes, Proteobacteria, and Bacteroidetes. Among them, Enterococcus viikkiensis may be a concern in the preservation. Eleven volatile organic compounds were identified from N. flagelliforme cells, in which geosmin could be treated as an indicator of the freshness of N. flagelliforme. The occurrence of indole compound may be an indicator of the degradation of cells.

• International Journal of Industrial Entomology and Biomaterials
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• 제46권2호
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• pp.41-54
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• 2023

The blue-tailed damselfly, Ischnura elegans Van der Linden, 1820 (Odonata: Coenagrionidae), is a climate-sensitive indicator species in South Korea. In this study, we sequenced the complete mitochondrial genome (mitogenome) of I. elegans collected from South Korea for subsequent population genetic analysis, particularly to trace population movements in response to climate change. The 15,963 base pair (bp)-long complete mitogenome of I. elegans has typical sets of genes including a major non-coding region (the A+T-rich region), and an arrangement identical to that observed in ancestral insect species. The ATP6, ND3 and ND1 genes have the TTG start codon, which, although rare, is the canonical start codon for animal mitochondrial tRNA. The A/T content was 71.4% in protein-coding genes, 72.1% in tRNAs, 72.9% in the whole genome, 74.7% in srRNA, 75.3% in lrRNA, and 83.8% in the A+T-rich region. The A+T-rich region is unusually long (1,196 bp) and contains two subunits (192 bp and 176-165 bp), each of which is tandemly triplicated and surrounded by non-repeat sequences. Comparison of the sequence divergence among available mitogenomes of I. elegans, including the one from the current study, revealed ND2 as the most variable gene, followed by COII and COI, suggesting that ND2 should be targeted first in subsequent population-level studies. Phylogenetic reconstruction based on all available mitogenome sequences of Coenagrionidae showed a strong sister relationship between I. elegans and I. senegalensis.

• Animal cells and systems
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• 제12권3호
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• pp.165-170
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• 2008

Cuticular hydrocarbons(CHCs) of the epicuticle wax layer are so far used to differentiate the insects in species and subspecies levels. In this study, four species of malaria vectors(genus Anopheles) were collected from various localities in southern Iran. Twenty specimens of each species were randomly selected and one epicuticular extract was prepared of every five specimens. FID-GC profiles of the extracts did not show any qualitative difference. Using significant difference of CHC mass at retention time(RT) 39.6, the two species of An. sacharovi and An. fluviatilis could be distinguished. Similarly, the two species of An. superpictus & An. sacharovi and An. dthali & An. sacharovi were differentiated by their CHC level at RT 28.5. An. sacharovi was distinguished by integratable peaks at RTs 29.7, 30.6, 30.7, 31 and 32.6 while the other three species just indicated trace peaks at the same RTs. Similarly, An. dthali could be known by an integratable peak at RT 26.2 while An. fluviatilis and An. superpictus indicated trace peaks at the same RT. Integratable peaks and traces at RTs 27.4 and 28.5 were respectively used to differentiate An. superpictus from An. fluviatilis. Lastly, CHC trace amount of An. superpictus at RT 39.6 is another indicator to distinguish it from An. fluviatilis with an integratable peak at the same RT. In harmony with other studies worldwide we hereby report that quantitative analysis of CHCs was successfully applied to differentiate the four Anopheles species of southern Iran.

• 생태와환경
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• 제53권3호
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• pp.286-294
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• 2020

깔따구(Diptera: Chironomidae)는 저서성 대형무척추동물로 환경오염 및 수질 모니터링에 이용되는 중요한 지표생물이다. 본 연구에서는 인천 수돗물 정수장에서 발견된 깔따구류의 정밀한 종 동정을 위해 형태적 분류와 미토콘드리아 DNA에서 cytochrome c oxidase subunit I (COI) 유전자의 염기서열을 이용하여 분석하였다. 정수장 6곳의 20개체는 안개무늬날개깔따구(Chironomus kiiensis) 12개체, 노랑털깔따구(Chironomus flaviplumus) 6개체, 등깔따구(Chironomus dorsalis) 1개체, 용산무늬깔따구(Polypedilum yongsanensis) 1개체 등 4종으로 확인되었다. 각 깔따구 종의 형태적 특징은 두부, 하순기절, 대악, 안테나, 발톱의 형태적 특징을 살펴보았다. NCBI Genbank에 등록된 깔따구 17종 21개체의 COI 염기서열을 바탕으로 본 연구에서 조사된 20개체의 계통진화적 분석한 결과 각 4종의 깔따구 COI 염기서열은 등록된 동인 종과 높은 상동성을 보이며 (99~100%) 같은 계통군(clade)으로 나타났다. 이러한 결과는 국내 깔따구의 종 동정을 위한 형태적- 유전적 정보를 통합적으로 제공함으로 담수생태계의 모니터링을 위한 주요한 정보로 활용될 것이다.

• Ocean and Polar Research
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• 제42권4호
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• pp.271-281
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• 2020

We investigated mesozooplankton in the Yellow Sea in spring to understand its community structure and relationship with environmental factors. Total mesozooplankton density ranged from 1,542 to 7,367 ind. m-3 and the biomass ranged from 3 to 42 mg C m-3. The total density and biomass had a positive relationship with chlorophyll-a (chl-a) concentration. The mesozooplankton community was divided into two groups at 125.5 E by cluster analysis: one was an inshore group and the other was an offshore group. The inshore group of mesozooplankton was of high density but low diversity, while the offshore group was of high diversity but low density. Copepod Acartia hongi and its copepodites were the most abundant species, comprising 27.8% of the total mesozooplankton density. A. hongi was especially abundant at the inshore, serving as the indicator species of the inshore group. Redundancy analysis found a positive relationship between the density of A. hongi and chl-a concentration. Oithona similis and Centropages abdominalis were 2nd and 3rd dominant species comprising 9 and 7% of the total density, respectively. The density of O. similis was positively related to water depth, but C. abdominalis was related to chl-a concentration. Chl-a concentration seems to influence significantly the mesozooplankton community structure in the Yellow Sea in spring, rather than water temperature or salinity.

• The Korean Journal of Ecology
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• 제10권2호
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• pp.63-80
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• 1987

Detrended correspondence analysis(DCA) and two way indicator species analysis(TWINSPAN) were applied to Pinus densiflora stands nearby Seoul area in order to classify them into several groups based on herbaceous species and environmental relationships. The eighteen Pinus densiflora stands were perferenially selected, In each stand, 30 to 60 quadrats, (20X20)cm, were randomly estabilished. In each quadrat, all stems for herbaceous plants were recorded by species and their covers were measured. In addition environmental factors such as soil depth, thickness of litter layer, soil compressibility, tree density, basal area, elevation, slope and exposure were detemined for each stand. Four to twelve soil cores were collected and physical and KDICical properties were determined. This survey was conducted form 15, July, 1985 to 22, September, 1985. Analysis of the vegetation and environmental data were performed with DECORANA and TWINSPAN. Stand ordination (DECORANA) and classification (TWINSPAN) based on herbaceous species suggest that the stands can be classified into five groups in terms of herbaceous species composition and environmental relationships. Type I, Type II, Type III, Type IV and Type V were classified to SUBMESIC-CLO-SED-CANOPY (Artemisia keiskeana, Carex nanella), MESIC-CLOSED CANOPY (Spodiopogon sibiricus, Miscanthus sinensis, Carex nanella, Pteridum aquilinum, Melampyrum rosium), SUBMESIC-OPEN CANOPY (Carex nanella, Spodiopogon sibiricus, Miscanthus sinensis), XERIC-CLOSED CANOPY (Carex nanella, Spodiopogon sibiricus, Carex nanella), and XERIC-OPEN CANOPY (Arundinella hirts, Spodiopogon sibiricus, Carex nanella), respectively. These results were consitent with environmental trends on stan ordination. Application of the ordination and classifiaction to Pinus densiflora stands were effective as a approach on obtaining perliminary ecological information.

• 한국산림과학회지
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• 제79권2호
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• pp.216-221
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• 1990

계룡산(鷄龍山)과 덕유산(德裕山) 삼림군집(森林群集)의 종(種) 조성(組成)과 입지(立地)와의 관계(關係)를 구명(究明)하기 위하여 TWINSPAN(two-way indicator species analysis)과 DCCA(detrended canonical correspondence analysis)를 시행하였다. 식생(植生) 및 환경자료(環境資料)로 계룡산(鷄龍山) 128개소(個所), 덕유산(德裕山) 57개소(個所)의 data를 사용(使用)하여 분석(分析)한 결과(結果)는 다음과 같다. 계룡산(鷄龍山)과 덕유산(德裕山)의 삼림군집(森林群集)과 토양환경(土壤環境)과의 관계(關係)를 살펴보면 Quercus mongolica군집(群集)은 해발고(海拔高)가 높고, 토양(土壤) 양료(養料)가 많은 곳에 주(主)로 분포(分布)하고 있으며, Carpinus laxiflora군집(群集)과 Fraxinus rhynchophylla군집(群集)은 해발고(海拔高)는 중간(中間)이고 양료(養料)가 많은 곳에 주(主)로 분포(分布)하고 있으며, Pinus densiflora-Quercus mongolica 군집(群集)과 Quercus variabilis 군집(群集)은 해발고(海拔高), 양료(養料) 모두 중간(中間) 부분(部分)인 곳에 주(主)로 분포(分布)하고 있으며, Styrax japonica 군집(群集)은 해발고(海拔高)는 낮고 양료(養料)가 중간부분(中間部分)인 곳에 주(主)로 분포(分布)하고 있으며, Pinus densiflora 군집(群集)은 해발고(海拔高)는 낮고 양료(養料)가 적은 부분(部分)에 주(主)로 분포(分布)하고 있다. 군집(群集) 분포(分布)에 영향을 미치는 제일(第一)의 환경(環境) 요인(要因)은 해발고(海拔高)(온도(溫度))이다.