• Research in Plant Disease
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• v.15 no.3
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• pp.254-258
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• 2009

An isolate of Cucumber mosaic virus (CMV), called as Lag-CMV, was identified from Lagenaria leucantha var. gourda showing mosaic symptom, and its properties was compared to Fny-CMV (subgroup IA) and As-CMV (subgroup IB) by host reaction in several indicator plants, dsRNA analysis, RT-PCR analysis, restriction enzyme profile of the PCR products and nucleotide sequence of coat protein gene. Lag-CMV was similar to As-CMV used as a control CMV by the induced chlorotic spot on inoculated leaves and mosaic symptoms on upper leaves of N. tabacum. cv. Xanthi nc. In the cucumber and zucchini squash, Lag-CMV and As-CMV induced a mild mosaic symptoms than that of Fny-CMV. Size and shapes of local lesions on Chenophodium amaranticolor and Vigna unguiculata induced by Lag-CMV was similar those by Fny-CMV or As-CMV. In experiments of dsRNA profiles and RT-PCR analysis of coat protein gene, Lag-CMV was come within subgroup I CMV. Moreover, restriction enzyme analysis using EcoRI, SalI, MspI, XhoI, and HindIII of the RTPCR products and nucleotide sequence analysis of the coat protein gene showed that Lag-CMV belong to a member of CMV subgroup IB of the same to As-CMV.

• Research in Plant Disease
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• v.23 no.2
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• pp.177-185
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• 2017

Bacillus amyloliquefaciens LM11 was isolated from the feces of larvae of the rhino beetle and showed strong antifungal activities against various phytopathogenic fungi by producing biosurfactants. In this study, our overall goal was to determine relationship between biosurfactants produced from the LM11 strain and its role in growth inhibition of phytopathogenic fungi. Production and expression levels of B. amyloliquefaciens LM11 biosurfactants were significantly differed depending on growth phases. Transcriptional and biochemical analysis indicated that the biosurfactants of the LM11 strain were greatly enhanced in late log-phase to stationary phase. Inhibitions of phytopathogenic mycelial growth and spore germination were directly correlated (P<0.001, R=0.761) with concentrations of the LM11 cell-free culture filtrates. The minimum inhibitory surface tension of the culture filtrate of the B. amyloliquefaciens LM11 grown in stationary phase to inhibit mycelial growth of the phytopathogenic fungi was 38.5 mN/m (P<0.001, R=0.951-0.977). Our results indicated that the biosurfactants of B. amyloliquefaciens LM11 act as key antifungal metabolites in biocontrol of plant diseases, and measuring surface tension of the cell-free culture fluids can be used as an easy indicator for optimal usage of the biocontrol agents.

• Journal of Wetlands Research
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• v.16 no.4
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• pp.353-362
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• 2014

The purpose of this study is to investigate the effect of micro-topography to vegetation pattern in Dunchon-dong wetland. To characterize the effect of micro-topography, changes in water level and vegetation pattern were monitored from 2007 to 2008. Depending on the relative elevation, the study site was divided into 4 sectors. The relative areas of sectors in ascending order were 11%, 10%, 18% and 24%, respectively. During investigation period, average water pH was 6.10 (${\pm}0.13$), electron conductivity was $51.5({\pm}6.0){\mu}s/cm$, $PO_4$-P, $NO_3$-N and $NH_4$-N concentration were $0.04({\pm}0.02)mg/L$, $0.14({\pm}0.07)mg/L$, and lower than 0.01mg/L, respectively. Water level was very changeable in low-water season because the area of lowest sector was small. This characteristic increased the effect of difference of accumulated precipitation from March to April in 2007 and 2008 to plant community composition in lower sectors. Different plant guilds dominated respective sectors and annual plants were major dominant species in the study site. This study suggested that the elevation gradients are necessary to create the habitats for various plant guilds in wetland.

• Korean Journal of Environmental Biology
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• v.32 no.3
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• pp.176-210
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• 2014

This scientific study was carried out to investigate the flora of Jecheon, located in the middle part of the Korean peninsula. We have surveyed the vascular plants of the above region from March to October 2014 (18 times). Totally, we identified 765 taxa; 127 families, 411 genera, 678 species, 9 subspecies, 70 varieties, 6 forms, and 2 hybrids. Endangered species designated by the law called' Protection Law for Endangered wild fauna and flora' were 6 taxa. The red data of vascular plants according to IUCN valuation basis comprised 29 taxa; 21 families, 26 genera, 29 species. 25 taxa were considered to be endemic to Korea. The floristic regional indicator plants found in this region were 122 taxa in total. Among them 12 taxa revealed the floristic degree V, 13 for floristic degree IV, 34 for floristic degree III, 11 for floristic degree II and 52 for floristic degree I. The naturalized plants were identified as 58 taxa. Further, we anticipate that the findings of this study survey would be useful for the scientific community to gain capacity to identify and conserve vascular plants of the region.

• Korean Journal of Plant Resources
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• v.28 no.1
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• pp.35-63
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• 2015

A floristic, field research was conducted from March 2005 to October 2012 the Woraksan National Park (N $36^{\circ}46^{\prime}{\sim}36^{\circ}59^{\prime}$, E $128^{\circ}02^{\prime}{\sim}128^{\circ}19^{\prime}$) to characterize the flora of the region. Especially, we divided the surveyed region into nine representative collection areas based on floristic components were compared and analyzed. This field study discovered significant plants in various categories. The 41 surveys revealed that the vascular plants in this region comprised 815 taxa: 116 families, 429 genera, 717 species, 4 subspecies, 78 varieties and 16 forms. In this study, 80 taxa were firstly recorded from this region. Korean endemic plants numbered 22 taxa, and the fifteen taxa rare plants designated by Korean Forest Service as were found in this region. The taxa in the category II of rare and endangered plants and the taxa with the higher rank than the third degree among the floristic regional indicator plants designated by Korean Ministry of Environment were one and 56, respectively. From a geographical perspective, limited distributions of Matteuccia struthiopteris, Spiraea trichocarpa, and Aster tataricus at a latitudes higher than that of Woraksan National Park. Moreover, this study has confirmed that the Woraksan National Park is the species diversity center for Asarum koreanum, Elsholtzia angustifolia, and Allium linearifolium in the central province of the Korean peninsula. Inaddition, the naturalized plants of 54 taxa were recorded.

• Journal of Korean Society of Forest Science
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• v.109 no.2
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• pp.124-135
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• 2020

Alder (Alnus japonica) forests are representative of the wetland in East Asia, including Korea. In the past, alder forests were relatively common in various habitats such as mountains, riversides, back marshes, and alluvial plains. However, this plant community has recently become rare due to increasingly arid habitats and the influence of various land uses. In this study, we identify the synecological characteristics of alder (A. japonica) forests distributed naturally in the mountainous wetlands of Korea and provide basic data for their systematic conservation and management in the future. Based on vegetation survey data collected from 66 alder forests, community types were classified using the methods of the Zürich-Montpellier School of Phytosociology and two-way indicator species analysis. There were eight community types: Styrax obassia, Weigela subsessilis-Fraxinus mandschurica, Spiraea fritschiana, Viola verecunda, Impatiens textori-Spiraea salicifolia, Glyceria leptolepis, Molinia japonica, and Lindera obtusiloba-Quercus acutissima. These community types constituted a vegetation unit hierarchy of two communities, four subcommunities, and eight variants. In addition, the ecological characteristics of each community type were compared (including total coverage per 100 square meter, importance value index, constancy class, life-form composition, diversity indices, community similarity coefficient, and indicator species).

• Journal of Environmental Impact Assessment
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• v.26 no.5
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• pp.291-302
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• 2017

The research was carried out in order to find climate factors which determine the distribution of Eurya japonica, and the potential habitats (PHs) under the current climate and climate change scenario by using species distribution models (SDMs). Four climate factors; the warmth index (WI), the minimum temperature of the coldest month (TMC), summer precipitation (PRS), and winter precipitaion (PRW) : were used as independent variables for the model. Seventeen general circulation models under RCP (Representative concentration pathway) 8.5 scenarios were used as future climate scenarios for the 2050s (2040~2069) and 2080s (2070~2099). Highly accurate SDMs were obtained for E. japonica. The model of distribution for E. japonica constructed by SDMs showed that minimum temperature of the coldest month (TMC) is a major climate factor in determining the distribution of E. japonica. The area above the $-5.7^{\circ}C$ of TMC revealed high occurrence probability of the E. japonica. Future PHs for E. japonica were projected to increase respectively by 2.5 times, 3.4 times of current PHs under 2050s and 2080s. It is expected that the potential of E. japonica habitats is expanded gradually. E. japonica is applicable as indicator species for monitoring in the Korean Peninsula. E. japonica is necessary to be monitored of potential habitats.

• Korean Journal of Ecology and Environment
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• v.53 no.3
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• pp.286-294
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• 2020

The Chironomus(Diptera: Chironomidae) is a freshwater benthic invertebrate that is an important indicator organism used for environmental pollution and water quality monitoring. In this study, we performed morphological classification and genetic species identification using the cytochrome c oxidase subunit I (COI) gene on mitochondrial DNA for an accurate species classification of Chironomus larvae found in tap water purification plants in Incheon, Korea. Twenty larvae in six water purification plants consist of four species, including twelve Chironomus kiiensis, six Chironomus flaviplumus, one Chironomus dorsalis, and one Polypedilum yongsanensis (not included Genus Chironomus). Morphological characteristics of each larvae were identified based on the head capsule, the mentum, the mandible, the antenna, and the claw. Based on the COI sequences of 21 individuals of 17 Chironomus species registered in NCBI Genbank, phylogenetic analysis indicated that the 20 individuals investigated in this study consist of the same clade with corresponding species of the high homology (99~100%) including C. kiiensis, C. flaviplumus, C. dorsalis, and P. yongsanensis. These results will be used as main classification indicator for monitoring freshwater ecosystems by providing integrated morphological and genetic information for the species identification of Korean Chironomus.

• Journal of Korean Society of Forest Science
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• v.87 no.2
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• pp.188-193
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• 1998

Soil pH change is an important factor which determines soil chemical properties. It is an indicator of the effect of urban environmental pollution on soils and plant growth. The objective of this study was to assess the effect of air pollution on the soil of Seoul urban forests during the course of the air pollution being dipersed toward suburb area. Study sites were divided into four sections of concentric circles with 5km interval. Soil samples were collected from A and B horizons in the urban forests, and analyzed for soil pH, soil buffer capacity, cation exchange capacity, and base saturation. Soil pH ranged from 3.96 to 5.08 for A horizon and from 4.10 to 5.25 for B horizon, which were not significantly different among the sections. However, there was a trend of soil pH lowered at the sections close to the urban center. Soil buffer capacity was lower at 0-5km and 5-10km sections compared to that at the outer sections. Cation exchange capacity and base saturation were not different significantly among the sections. Following the pattern of air pollutants being dispersed from urban center to suburb, soil acidification was observed at the urban forests in Seoul area. Low level of soil buffer capacity toward the urban center was an indicator of soil acidification at the urban forests.

• Journal of Wetlands Research
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• v.14 no.4
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• pp.699-713
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• 2012

Palustrine wetland is often used for development work and green area creation. However, the ecological characteristic of vegetation is not frequently considered in planning. Therefore, this study was conducted to investigate vegetation characteristic according to land-use of palustrine wetland and to provide basic materials in assessment of palustrine wetland and restoration and creation of wetland. Survey sites were caterogized into paddy, field, and mountain. Each category had two sites, so total 6 survey sites were investigated for vegetation. As a result, with a range of 61~92 species, total 216 taxa were confirmed, consisting of 71 families, 168 generic, 187 species, 2 variety 1 forma 1 subforma. Mountain received a high score in the evaluation score range of floristic regional indicator plants, and it was found that there were 10 families and 28 species of naturalized plant. Mountain showed low appearance, and fiddy and field showed relatively high appearance. Naturalized plants with slightly high vegetation cover ratio are Erigeron annuus, Amorpha fruticosa, Dactylis glomerata, Festuca arundinacea, Eragrostis curvula, Trifolium repens, Erigeron Canadensis, Ambrosia trifida, Oenothera odorata, Bidens frondosa, etc. Such naturalized plants should be removed to prevent terrestrialization of wetland and its harmful damages. Long-term monitoring and proper management of naturalized plants are expected to improve palustrine wetland into high quality biotope of rural area in the future.