• The Plant Pathology Journal
• /
• 제21권2호
• /
• pp.106-110
• /
• 2005

Sclerotinia sclerotiorum attacks most of the vegetable crops in the Jordan valley. Twenty-five samples/isolates were obtained in a complete coverage of that region. They were characterized for their mycelium incompatibility, and specific gene amplified using the primer SSREV/SSFWD. All isolates gave similar single band around 278 bp. Thirteen isolates were completely incompatible with the other 12 ones. The latter ones fell into four subgroups of mycelium incompatibility. RAPD analysis using three primers (OPA-2, OPA-10, and OPA-18) clustered the 25 isolates into subgroups in agreement with their morphological separation, indicating close correlation between amplified gene(s) and the gene(s) of incompatibility. All highly virulent isolates were among the group of 13, indicating a well established genomic type pathogen in this region.

• 한국작물학회:학술대회논문집
• /
• 한국작물학회 2022년도 추계학술대회
• /
• pp.191-191
• /
• 2022

Heterostyly continues to fascinate evolutionary biologists interested in heredity, evolution, breeding, and adaptive function. Polymorphism demonstrates how simply inherited developmental changes in the location of plant sexual associations can have important consequences for population pollination and mating biology. In contrast to homozygous self incompatibility, only a small number of mating phenotypes can be maintained in the population because insect pollinators have limitations in achieving multiple segregation sites for pollen deposition. Field studies of pollen tube growth have shown that reciprocal style-stamen polymorphisms function to increase the capacity of insect-mediated cross-pollination. The genetic pattern of style morphs is well established in various taxa, but despite recent advances, the identity, number, and structure of the genes controlling the heteromorphic syndrome have been poorly elucidated. The phenomenon of heterostyly in buckwheat has been controlled by gene complex concentrate to S-locus. Homomorphic autogamous buckwheat strains were established by the interspecific hybridization. Backcrossing of this line to the common buckwheat (pin) and selecting homostylar progenies made it possible to introduce the self-compatible gene into common buckwheat. In the result, we obtained the BC₉F₂ generation, and defined the strong linkage between flower type and self-incompatibility by microscopic observation of pollen tube growth. This finding suggests that self-incompatibility character is not controlled by one gene. Moreover, we defined the strong linkage between flower type and self-incompatibility. It strongly supports the S supergene theory. Therefore, we have plan to elucidate the heterostyly self-incompatibility by using molecular genetics, proteome analysis and apply to exploitation of buckwheat improvement. In near future, the expression of heterozygous syndromes in genus Fagopyrum with single isolated heterozygous species may provide clues to early stages of polymorphic assembly and shed light on evolutionary models of heterozygous strains.

• BMB Reports
• /
• 제30권1호
• /
• pp.18-20
• /
• 1997

Lycopersicon peruvianum shows a gametophytic self-incompatibility (GSI). GSI is controlled by a single locus (S locus) with multiple alleles. S ribonucleases encoded in S alleles cosegregate with their phenotypes of GSI in genetic cross. To understand the genetic role of S allele in L peruvianum, two large genomic fragments isolated previously were analyzed with total genomic DNAs from several tomato lines generated by cross-pollination. Southern blot analysis with the S allele fragments as probes revealed that the flanking region of S allele contained the highly homologous regions. It is speculated that they may play an important role to prevent genetic cross by self-pollination.

• 가정과삶의질연구
• /
• 제8권1호
• /
• pp.49-67
• /
• 1990

The purpose of this study is to investigate the relations between marital status incompatibility and the ways of dealing with marital conflict. Research was conducted by interview using questionnaire on 177 employed wives. Statistics used for data analysis were frequency distribution, percentage, t-test, chi-square test. 1) In resolving marital conflict four types of method are appeared, namely constructive method, passive method, evasion and physical violence. 2) Only physical violence of husband-to-wife of the four methods is related to status in-compatibility. That is the incidence rate of physical violence of husband-to-wife in incompatible couples is higher than that of compatible couples in conflict situations. 3) In case the wife's occupational status is high relative to her husband the incidence rate of physical violence of husband-to-wife is extremely high, but is significantly low in couples which are compatible in this status. 4) In case wife's status advance ent is controlled, there is significant relationship between status incompatibility and the method of conflict resolution by husband whose wife is status striver.

• Journal of Plant Biotechnology
• /
• 제37권2호
• /
• pp.186-195
• /
• 2010

In most self-incompatible plant species, recognition of self-pollen is controlled by a single locus, termed the S-locus. The self-incompatibility (SI) system in Brassica is controlled sporophytically by multiple alleles at a single locus, designated as S, and involves cell-cell communication between male and female. Two highly polymorphic S locus genes, SLG (S locus glycoprotein) and SRK (S receptor kinase), have been identified, both of which are expressed predominantly in the stigmatic papillar cell. Gain-of-function experiments have demonstrated that SRK solely determines S haplotype-specificity of the stigma, while SLG enhances the recognition reaction of SI. The sequence analysis of the S locus genomic region of B. campestris (syn. rapa) has led to the identification of an anther-specific gene, designated as SP11/SCR, which is the male S determinant. Molecular analysis has demonstrated that the dominance relationships between S alleles in the stigma were determined by SRK itself, but not by the relative expression level. In contrast, the expression of SP11/SCR from the recessive S allele was specifically suppressed in the S heterozygote, suggesting that the dominance relationships in pollen were determined by the expression level of SP11/SCR. Furthermore, recent studies on recessive allele-specific DNA methylation of Brassica self-incompatibility alleles demonstrate that DNA methylation patterns in plants can vary temporally and spatially in each generation. In this review, we firstly present overview of self incompatibility system in Brassica and then describe dominance relationships in Brassica self- incompatibility regulated by allele-specific DNA methylation.

• 한국균학회지
• /
• 제14권1호
• /
• pp.17-23
• /
• 1986

여름느타리버섯 Pleurotus sajor-caju은 2개(個)의 불화(不和) 합성(合性) 인자(因子)를 가진 4극성(極性) 교배계(交配系)를 가지고 있으며, 2핵균사체(核菌絲體)는 단핵균사체(單核菌絲體)보다 균사생장(菌絲生長)이 빨랐으나 상호(相互) 상관관계(相關關係)는 없었다. 여름느타리버섯은 2핵균사체(核菌絲體) 뿐만아니라 단핵균사체(單核菌絲體)도 agar배지(培地) 상(上)에서 원기형성(原基形成)이 잘되는 특징(特徵)이 있으며 2핵균사체(核菌絲體)에서 그 형성수(形成數)가 많은 경향(傾向)이었다. 단핵균사체(單核菌絲體)의 자실체(子實體)는 그 형태(形態)가 기형(奇型)이었으며 성숙(成熟)하지 못하고 사멸(死滅)되었으나 2핵균사체(核菌絲體)의 자실체(子實體)는 정상(正常)으로 생장(生長)하였다. 교배균주(交配菌株) 중(中) D-5 균주(菌株)는 모균주(母菌株) 보다 자실체(子實體) 수량(收量)이 높았다. Agar배지(培地) 상(上)에서 2핵균사체(核菌絲體)의 균사생장(菌絲生長)과 원기형성(原基形成) 수(數)는 톱밥 병재배(甁栽培)로 얻은 자실체(子實體) 수량(收量)과 고도(高度)의 상관(相關)이 있었으며, 자실체(子實體) 수량(收量)은 산(傘)의 직경(直徑)과 줄기의 길이와도 고도(高度)의 상관(相關)을 보였다.

• 한국균학회소식:학술대회논문집
• /
• 한국균학회 2009년도 추계학술대회 및 정기총회
• /
• pp.27-28
• /
• 2009

• 한국균학회소식:학술대회논문집
• /
• 한국균학회 2009년도 추계학술대회 및 정기총회
• /
• pp.14-16
• /
• 2009

Poria cocos is an importantant medical macrofungus,the sclerotium of Poria cocos has specific value as the drug material. There are few papers about its breeding and spawn rejuvenation. In this project, the protoplasts of cultivated strain T and wild strain L were prepared and treated separately by ultraviolet and heating, then fused with the PEG6000. The tural fusants were selected and identified by the affinity and ISSR analysis. 71 incompatibility strains between parents and reg regenerations were obtained from 118 regenerations by the affinity analysis. Five incompatibility strains were amplified with different primers, the results were showed that they had specific bands of both parents in the profile amplified with 3 primers, which proved these 5 strains were fusants by means of molecular biology marker. On the other hand, 25 strain were selected from 168 protoplast regenerations of cultivated strain T for cultivation experiment. The fresh sclerotium weight of these protoplast regenerations were better than the original strain.significantil 3 strains (T-1, T-4, T-7) increased respectively 118%, 73% and 73% than original strain. This method could be the effective in the rejuvenation Poria cocos.

• 한국육종학회지
• /
• 제41권4호
• /
• pp.397-402
• /
• 2009

Self-incompatibility (SI) prevents self-fertilization by inhibiting the pollen tube growth of self-pollen. Molecular analysis has revealed that the S locus comprises a number of genes, such as the S-locus glycoprotein (SLG), the S-locus receptor kinase (SRK), and SP11 (SCR). Although molecular markers related to those genes have been developed, a simple S-haplotype detecting method has not been reported due to the highly polymorphic and relatively small coding regions. In this study, the sequence characterized amplified region (SCAR) markers were used to establish an efficient radish genotyping method. We identified the S-haplotypes of 192 radish accessions using 19 different markers, which proved to be highly reliable. The accessions were assigned to 17 types of S-haplotypes, including 8 types of SRKs and 9 types of SLGs. Since the developed SCAR markers are based on their gene sequences, we could easily identify the S-haplotypes by a single specific band, with the highest frequencies detected for SLG 5, SRK 1, and SLG 1, in order. Among the tested markers, the SLG 1, SRK 1, and SRK 5 markers exhibited high reliability, compared to phenotypic results. Furthermore, we identified the seven types of unreported SLGs using SLG Class -I and -II specific markers. Although the developed SCAR markers still need to be improved for the genotyping of all S-haplotypes, these markers could be helpful for monitoring inbred lines, and for developing the MAS in radish breeding programs.

• KSII Transactions on Internet and Information Systems (TIIS)
• /
• 제15권8호
• /
• pp.2805-2826
• /
• 2021

In most cases, the routing policy of networks shows a preference for a static one-to-one mapping of communication pairs to routing paths, which offers adversaries a great advantage to conduct thorough reconnaissance and organize an effective attack in a stress-free manner. With the evolution of network intelligence, some flexible and adaptive routing policies have already proposed to intensify the network defender to turn the situation. Routing mutation is an effective strategy that can invalidate the unvarying nature of routing information that attackers have collected from exploiting the static configuration of the network. However, three constraints execute press on routing mutation deployment in practical: insufficient route mutation space, expensive control costs, and incompatibility. To enhance the availability of route mutation, we propose an OpenFlow-based route mutation technique called Polymorphic Path Transferring (PPT), which adopts a physical and virtual path segment mixed construction technique to enlarge the routing path space for elevating the security of communication. Based on the Markov Decision Process, with considering flows distribution in the network, the PPT adopts an evolution routing path scheduling algorithm with a segment path update strategy, which relieves the press on the overhead of control and incompatibility. Our analysis demonstrates that PPT can secure data delivery in the worst network environment while countering sophisticated attacks in an evasion-free manner (e.g., advanced persistent threat). Case study and experiment results show its effectiveness in proactively defending against targeted attacks and its advantage compared with previous route mutation methods.