• Journal of Microbiology and Biotechnology
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• 제22권2호
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• pp.234-238
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• 2012

Recombinant Escherichia coli displaying organophosphorus hydrolase (OPH) was used to overcome the diffusion barrier limitation of organophosphorus pesticides. A new anchor system derived from the N-terminal domain of ice-nucleation protein from Pseudomonas syringae InaV (InaV-N) was used to display OPH onto the surface. The designed sequence was cloned in the vector pET-28a(+) and then was expressed in E. coli. Tracing of the expression location of the recombinant protein using SDS-PAGE showed the presentation of OPH by InaV-N on the outer membrane, and the ability of recombinant E. coli to utilize diazinon as the sole source of energy, without growth inhibition, indicated its significant activity. The location of OPH was detected by comparing the activity of the outer membrane fraction with the inner membrane and cytoplasm fractions. Studies revealed that recombinant E. coli can degrade 50% of 2 mM chlorpyrifos in 2 min. It can be concluded that InaV-N can be used efficiently to display foreign functional protein, and these results highlight the high potential of an engineered bacterium to be used in bioremediation of pesticide-contaminated sources in the environment.

• 한국결정학회:학술대회논문집
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• 한국결정학회 2005년도 추계학술연구발표회 및 정기총회
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• pp.19-19
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• 2005

• 한국주거학회논문집
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• 제19권6호
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• pp.137-144
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• 2008

Public housing problem became a major issue in each European country during the period of the reconstruction. This study focuses on the one of the major institutional policies in the public housing sectors in Europe during the second postwar period, specifically the INA (Istituto Nazionale delle Assicurazioni)-Casa in Italy from 1949 to 1963. Thanks to the financial Aid provided by 'Marshall Plan' of United States government, Italy initiated INA-Casa housing program elaborated and realized by minister of Labour, Amintore Fanfani. He was strongly influenced by catholic socialism and Weberian corporatism, and also had previously written theoretical studies on the subject during the Interwar years. The outcome of this policy had been devaluated for its Keynesian aspect as well as vernacular predominance in the architectural forms. On the contrary, this study focuses on themes such as continuity of politics before and after the Liberation, status of the architects and their will to social engagement, notion of quarter as 'self-sufficient community' and 'residential unit'. These factors will allow to comprehend the complex nature of the reality where different categories were involved to resolve the common housing problem.

• 기록학연구
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• 제58호
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• pp.95-145
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• 2018

커뮤니케이션 언어로서 텍스트보다 사진, 이미지, 영상 등 비(非)텍스트 기록의 중요성이 커지고 있다. 이에 따라 국내 기록관리 유관 기관과 전문가들 또한 이미지 기록의 특수성에 적합한 기록관리 체계 마련의 필요성에 공감하고 있다. 이 글은 2018 국제 영상기록 관리연수(INA Frame)에 참가한 경험을 토대로 국내 영상기록관리 체계 마련을 위해 고민해야 할 지점들을 정리한 글이다. 특히 국가적 차원에서 관리해야 할 대상을 단순한 영화, 방송, 문화콘텐츠 등을 포괄하는 다양한 유형의 콘텐츠에서 더 나아가 '공공영상문화유산'의 개념정립의 필요성에 대해 역설하였다. 또한, 관련 제도 인프라 정비, 기관 또는 거버넌스 설계 등과 같은 '기반구축'에 해당하는 여러 과제들을 검토하여 이 선결과제들이 향후 국내에서 안정적인 '공공영상문화유산' 관리체계 마련으로 이어져야 함을 강조한다.

• 수완나부미
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• 제1권1호
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• pp.135-143
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• 2009

• Restorative Dentistry and Endodontics
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• 제21권1호
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• pp.425-435
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• 1996

The effect of application of dentin bonding agent to the exposed dentin on the intradental nerve activity (INA), dentinal fluid movement and sealing of the dentinal tubules, was investigated in this study. The INA was recorded from the single pulp nerve unit dissected from the inferior alveolar nerve. And specimen of dentin was observed by SEM. Dentinal fluid 'movement through exposed dentin surface was measured before and after the application of dentin bonding agent. 1. Eight Ao-fiber units (conduction velocity: $8.0{\pm}4.0m$/sec) were identified. 4M NaCl evoked an irregular burst of action potentials which ceased immediately after washing. 2. In 4 $A{\delta}$-fiber units, appliction of All Bond 2 completely abolished the INA induced by 4M NaCl. Also, application of Scotchbond Multipurpose(SBMP) totally abolished the INA induced by 4M NaCl in 4 $A{\delta}$-fiber units. 3. Before the application of dentin bonding agent, outward dentinal fluid movement of $10.2{\pm}5.7\;pl{\cdot}s^{-1}{\cdot}mm^{-2}$ was obsered. But after the application of dentin bonding agent the movement of dentinal fluid was stopped. 4. The gap width of 2-$3{\mu}m$ was formed between exposed dentin and adhesive resin in the specimens applied with dentin bonding agents of All Bone 2 and SBMP. But the formation of hybrid layer and the penetration of resin into were dentinal tubules were not clearly observed in interface between dentin and adhesive resin.

• Restorative Dentistry and Endodontics
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• 제21권2호
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• pp.505-516
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• 1996

The effects of application of dentin desensitizer containing glutaraldehyde (Gluma Desensitizer) and dentin adhesive system (All Bond 2) to the exposed dentin on the intradental nerve activity (INA) and the occluding aspects of dentinal tubules were investigated in cat canine teeth. Single pulp nerve units were dissected from the inferior alveolar nerve and indentified as $A{\delta}$-fiber units. The INAs elicited by 4M NaCl before and after the application of each experimental agent were compared. The morphological changes of exposed dentin surfaces and dentinal tubules in the dentin specimens used to record INAs were observed by SEM. The results obtained were as follows. 1. Eight $A{\delta}$-fiber units (conduction velocity: $8.0{\pm}4.0m$/sec) were identified. 4M NaCl evoked an irregular burst of action potentials which ceased immediately after washing. 2. In 4 $A{\delta}$-fiber units, the change of INA after the application of Gluma Desensitizer was $133.9{\pm}80.7%$ when it was compared with the INA before the application of the same agent. 3. In 4 $A{\delta}$-fiber units, application of All Bond 2 completely abolished the INA induced by 4M NaCl. 4. In specimens applied with Gluma Desensitizer, the formation of hybrid layer as well as the identification of resin penetration and reaction products with proteins in dentinal tubules were not clearly observed in interface between dentin and adhesive resin. In specimens applied with All Bond 2, the gap width of 2-$3{\mu}m$ was formed between exposed dentin and adhesive resin, and the hybrid layer and resin tags were not clearly formed either.

• 농업생명과학연구
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• 제46권4호
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• pp.113-118
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• 2012

생리활성물질 처리가 priming 고추종자의 발아에 미치는 영향을 분석한 결과, ASM 0.1 mM 처리에서는 치상후 7일째 98%의 발아율을 보였으며 T50은 0.96일이였다. 그러나 ASM 0.5 mM 처리에서는 17%의 낮은 발아율을 보여 발아가 억제되었다. INA 0.01 mM 처리에서는 치상 후 2일째에 90% 이상의 발아율을 보였지만, 0.1 mM 처리에서는 치상 후 5일째에 90%의 발아율을 보였다. T50은 INA 0.01과 0.1 mM 처리에서 각각 0.65와 6.03일로 나타났다. BABA와 JA 처리는 priming에 의한 발아촉진 효과에 영향을 미치지 않았다.

• 한국잠사곤충학회지
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• 제20권2호
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• pp.32-35
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• 1978

현장려잠품종에 대하여 FV 및 이나주바이러스의 2령기잠 경구접종에 의한 품종간저항성을 비교검정한 결과는 다음과 같다. 1. FV 에 대한 품종별 저항성은 한생 1 호 $\times$ 한생 2 호가 가장 강하였고 무등$\times$선호가 가장 약했다. 2. 이나주 바이러스에 대한 저항성은 잠 117$\times$잠118, 경추$\times$소일, 무등$\times$선호, 한생 1호$\times$한생 2호 및 한생 3호$\times$4호 등 5개 품종은 완전저항성을 나타냈으며 잠 115$\times$잠116이 가장 약했다.

• Membrane and Water Treatment
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• 제13권6호
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• pp.321-335
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• 2022

Copper-based Metal-organic framework (MOF) namely ([Cu (INA)₂]-MOF) is synthesized by ball milling and characterized using scanning electron microscopy (SEM) for the topography, microstructure, and elemental evidence determination, powdered X-ray diffraction (XRD) for the crystallinity measurement, thermogravimetric (TG) analysis was performed to determine the thermal stability of the material, and Fourier transformed infrared (FTIR) spectroscopy for functional groups identification. The use of [Cu (INA)₂]-MOF as hazardous removal material of β-agonists as persistent hazardous micro-pollutants in our environmental water is first reported in this study. The removal efficiency of the Cu-MOF is successfully determined to be 97.7% within 40 minutes, and the MOF has established an exceptional removal capacity of 835 mg L^-1 with 95 % percent removal on Clenbuterol (CLB) even after the 5th consecutive cycle. The Langmuir model of the adsorption isotherms was shown to be more favourable, while the pseudo-second-order model was found to be favoured in the kinetics. The reaction was exothermic and spontaneous from a thermodynamic standpoint, and the higher temperatures were unfavourable for the adsorption study of the CLB. As a result, the studied MOF have shown promising properties as possible adsorbents for the removal of CLB in wastewater.