• Journal of Embryo Transfer
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• v.28 no.3
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• pp.207-213
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• 2013

This study was carried out to examine a molecular marker system for parentage test in Jeju Black cattle (JBC). Based on the preliminarily studies, we finally selected for construction of a novel genetic marker system for molecular traceability, identity test, breed certification, and parentage test in JBC and its related industrial populations. The genetic marker system had eight MS markers, five indel markers, and two single nucleotide polymorphisms (SNPs; g.G299T and g.del310G) within MC1R gene which is critical to verify the breed specific genotypes for coat color of JBC differing from those of exotic black cattle breeds such as Holstein and Angus. The results showed lower level of a combined non-exclusion probability for second parent (NE-P2) of $4.1202{\times}10^{-4}$ than those previously recommended by International Society of Animal Genetics (ISAG) of $5.000{\times}10^{-4}$ for parentage, and a combined non-exclusion probability for sib identity (NE-SI) of $2.679{\times}10^{-5}$. Parentage analysis has been successfully identified the JBC offspring in the indigenous population and cattle farms used the certified AI semens for production using the JBC-derived offspring for commercial beef. This combined molecular marker system will be helpful to supply genetic information for parentage test and traceability and to develop the molecular breeding system for improvement of animal productivity in JBC population.

• Journal of The Geomorphological Association of Korea
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• v.20 no.4
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• pp.15-28
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• 2013

This paper was made on the basis of geotourism to explain morphological characteristics of Patagonia and Atacama region in the Andes through 10-day fieldwork. The Patagonian landscape was focused on the Torres del Paine National Park, Chile and the Los Glacier National Park, Argentina; on the other hand, the Atacama morphological landscape was investigated around the San Pedro de Atacama. Many geomorphological features such as uplift, semiarid and glacier landscapes can be found in Patagonia, so it is strongly recommended to have an opportunity to enjoy these landforms by geotourism. Massive intrusive rock bodies of granite can be shown in those two National Parks declared a Biosphere Reserve by UNESCO. And, the Atacama Desert located on the forearc depression in the Andes, Chile has also many geomorphological destinations of arid and volcanic landforms; badland, pediplain, pediment, salt lake and geyser. Especially, huge pediplain is one of the greatest view points in the Atacama Desert. Patagonia and Atacama are the best geographical tourism sites to know diverse tectonic landforms made by the conflict between South American and Pacific plates.

• 한국균학회소식:학술대회논문집
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• 2015.05a
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• pp.41-41
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• 2015

BSA-seq technologies, combined Bulked Segregant Analysis (BSA) and Next-Generation Sequencing (NGS), are making it faster and more efficient to establish the association of agronomic traits with molecular markers or candidate genes, which is the requirement for marker-assisted selection in molecular breeding. Clubroot disease, caused by Plasmodiophora brassicae, is a serious threat to Brassica crops. Even we have breed new clubroot resistant varieties of Chinese cabbage (B. rapa ssp. pekinesis), the underlying genetic mechanism is unclear. In this study, an $F_2$ population of 340 plants were inoculated with P. brassicae from Xinye (Pathotype 2 on the differentials of Williams). Resistance phenotype segregation ratio for the populations fit a 3:1 (R:S) segregation model, consistent with a single dominant gene model. Super-BSA, using re-sequencing the parents, extremely R and S DNA pools with each 50 plants, revealed 3 potential candidate regions on the chromosome A03, with the most significant region falling between 24.30 Mb and 24.75 Mb. A linkage map with 31 markers in this region was constructed with several closely linked markers identified. A Major QTL for clubroot resistance, CRq, which was identified with the peak LOD score at 169.3, explaining 89.9% of the phenotypic variation. And we developed a new co-segregated InDel marker BrQ-2. Joint BSA-seq and traditional QTL analysis delimited CRq to an 250 kb genomic region, where four TIR-NBS-LRR genes (Bra019409, Bra019410, Bra019412 and Bra019413) clustered. The CR gene CRq and closely linked markers will be highly useful for breeding new resistant Chinese cabbage cultivars.

• Journal of Plant Biotechnology
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• v.42 no.4
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• pp.326-335
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• 2015

Citrus is an economically important fruit tree with the largest amount of fruit production in the world. It provides important nutrition such as vitamin C and other health-promoting compounds including its unique flavonoids for human health. However, it is classified into the most difficult crops to develop new cultivars through conventional breeding approaches due to its long juvenility and some unique reproductive biological features such as gamete sterility, nucellar embryony, and high level of heterozygosity. Due to global warming and changes in consumer trends, establishing a systematic and efficient breeding programs is highly required for sustainable production of high quality fruits and diversification of cultivars. Recently, reference genome sequences of sweet orange and clementine mandarin have been released. Based on the reference whole-genome sequences, comparative genomics, reference-guided resequencing, and genotyping-by-sequencing for various citrus cultivars and crosses could be performed for the advance of functional genomics and development of traits-related molecular markers. In addition, a full understanding of gene function and gene co-expression networks can be provided through combined analysis of various transcriptome data. Analytic information on whole-genome and transcriptome will provide massive data on polymorphic molecular markers such as SNP, INDEL, and SSR, suggesting that it is possible to construct integrated maps and high-density genetic maps as well as physical maps. In the near future, integrated maps will be useful for map-based precise cloning of genes that are specific to citrus with major agronomic traits to facilitate rapid and efficient marker-assisted selection.

• Food Science of Animal Resources
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• v.30 no.2
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• pp.291-297
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• 2010

This study was carried out to investigate antioxidative and antimicrobial activities of ethanol extracted garlic stem and red cabbage, and their application to pork patties during refrigerated storage. Total phenolic compounds of ethanol extracted garlic stem and red cabbage were 3.13 g/100 g and 3.99 g/100 g, respectively. 1,1'-diphenyl-2-picryhdrazyl (DPPH) radical scavenging activity of garlic stem extracts increased up to 0.05%. Iron chelating activities of both garlic stem and red cabbage increased up to 0.5%, and red cabbage up to the concentration of 1% had similar iron chelating activity to those of EDTA. Pork patties containing garlic stem had higher Hunter L and b values, (p<0.05), whereas those with red cabbage had highest Hunter a values, resulting in similar value to those of control (p>0.05). Thiobarbituric acid reactive substances (TBARS) of pork patties with garlic stem were lower than those of the control (p<0.05). In addition, antioxidantive activities of pork patties with red cabbage were higher than those with garlic stem. No differences in microbial contents were observed between the control and treatments. In conclusion, garlic stem and red cabbage extracts might be used as natural antioxidants to be used in the manufacture of functional meat products.

• Journal of International Area Studies (JIAS)
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• v.14 no.3
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• pp.223-240
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• 2010

In this article I would like to propose the characteristics of "Village Council(Consejo del Pueblo)" as a sociopolitical device and institution, which maintains and encompasses all aspects of governance in a decentralized authority. A brief definition of Village Council is a "select group of people from the same village who are in charge of deciding the most important affairs of the village". The Village Council took charge of the most fundamental aspects of prehispanic Mayan society, most significantly, during the Late Postclassical era. The members of this council were respected people from the village, but not necessarily the people who held high positions within the hierarchical structure of Mayan society. It is likely that they were selected by the public in a direct way. This is the most essential bureaucratic institution in terms of the control of the political and socioeconomical affairs. The important decisions regarding legislation, the governance and jurisdiction of the village were entrusted to the council. Especially, the Village Council was responsible for monitoring and punishing the abuses of village governors. The majority of the members of the council were elders, consequently, the council was a political institution, more powerful than the governorships. The "Multepal" of Yucatán, could be an example of the Village Council during the late postclassic period.

• Journal of The Korean Society of Inherited Metabolic disease
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• v.16 no.2
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• pp.109-114
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• 2016

Carbamoyl phosphate synthetase 1 (CPS1) deficiency is an autosomal recessive urea cycle disorder which causes hyperammonemia. CPS1 is the first enzyme step in the urea cycle and almost patients present their symptoms during neonatal period. We report a case of CPS1 deficiency in a boy who developed symptoms including lethargy and seizure at 3 days of age. The ammonia level was up to $2,325{\mu}mol/L$, sodium benzoate (250 mg/kg/d) and high calories of both dextrose and lipid was promptly administered. Central access by experienced pediatric surgeon and emergent continuous hemodialysis by pediatric nephrologist was performed within 3 hours and ammonia was less than $100{\mu}mol/L$ at 5 days of age. Currently, he has showed excellent response to treatments including scavenging drugs and a low-protein diet. Despite of diffuse increasing signal intensity on cerebral white matters and basal ganglia on brain MRI, his development and weight gain were good at the last follow-up at 11 months of age. Molecular assay of the CPS1 gene demonstrated that patient had compound heterozygous for c.1529del ($p.Gly510Alafs^*5$) in exon 14 and c.3142-1G>C (IVS25(-1)G>C) in intron 25 and exon 26 boundary. The splicing mutation was novel mutation and inherited from patient's mother. Here, we report a neonatal lethal type CPS1 deficiency patient having novel mutation.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.6 no.7
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• pp.993-1000
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• 2002

In this paper, we design the CORBA-based Mobile Agent Model (CMAM) which has the capability of the efficient work processing in distributed environment through sensing network traffic and searching the optimal path for migration nodes of mobile agent. In case existing mobile agent model is given various works from user, the network overhead and traffic are increased by increasing of execution module size. Also, if it happens a large quantity of traffics due to migration of nodes according to appointment of the passive host(below node) routing schedule by user, it needs much cost for node search time by traffic. Therefore, in this paper, we design a new mobile agent m()del that assures the reliability of agent's migration through dynamic act on the specific situation according to appointment of the active routing schedule and can minimize agent's work processing time through optimal path search. The proposed model assigns routing schedule of the migration nodes actively using an extended MAFFinder. Also, for decreasing overhead of network by agent's size, it separates the existing mobile agent object by mobile agent including only agent calling module and push agent with work executing module based on distributed object type of CORBA. Also, it reduces the required time for round works of mobile agent through the optimal path search of migration nodes.

• Asian Pacific Journal of Cancer Prevention
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• v.15 no.17
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• pp.7195-7200
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• 2014

Background: The aim of this study was to evaluate how CYP2C19 affects icotinib and metabolite' exposure, and to determine whether the exposure and EGFR genotype influences survival time, tumor metastasis and adverse drug reactions. Materials and Methods: 274 NSCLC patients who accepted 125mg icotinib/t.i.d. were chosen from a phase III study. Blood samples were obtained in $672^{nd}$ ($4^{th}$ week) and $1,680^{th}$ hours ($10^{th}$ week), and plasma was used to quantify the concentration of icotinib and blood cells were sampled to check the genotypes. Clinical data were also collected at the same time, including EGFR genotypes. Plasma concentrations were assessed by HPLC-MS/MS and genotype by sequencing. All data were analyzed through SPSS 17.0 and SAS 9.2. Results: CYP 2C19 genotypes affected bio-transformation from icotinib to M24 and M26, especially in poor-metabolisers. Higher icotinib concentrations (>1000 ng/mL) not only increased patient PFS and OS but also reduced tumor metastasis. Patients with mutant EGFR experienced a higher median PFS and OS (234 and 627 days), especially those with the 19del genotype demonstrating higher PR ratio. Patients who suffered grade II skin toxicity had a higher icotinib exposure than those with grade I skin toxicity or no adverse effects. Liver toxic reactions might occur in patients with greater M20 and M23 plasma concentrations. Conclusions: CYP2C19 polymorphisms significantly affect icotinib, M24 and M26 exposure. Patients with mutant EGFR genotype and higher icotinib concentration might have increased PFS and OS and lower tumor metastasis. Liver ADR events and serious skin effects might be respectively induced by greater M20, M23 and icotinib concentrations.

• Journal of the Korean Society for Library and Information Science
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• v.30 no.2
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• pp.149-165
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• 1996

Hong-sii Tokso-rok (홍씨독서록 or Hong's Annotated Bibliography of Korean and Chinise Book) is the only work on the history of Korean bibliographies that has the introductory notes to each class, that is description of the origin of subject fields, transition, and characteristics at the beginning of each class. This paper is aimed to examine the outline of the introductory description of class, to analyze the Four Category Classification System(사부분류법) devised by Hong Sok-chu, and to explain how the classes of Four Category Classification are set and ordered. This paper shows several characteristics in the idea of Hong's classification system. There characteristics were discovered by analyzing the content of each introduction of classes. The characteristics ale as follows First, classes are organize and arranged from the substantial problem to nonsubstantial ones. In other words, the greater the distance of the class from the substantial problem of Confucianism, the farther the order of the class will be found from the substantial problem. The order of classes is set by how the class is closed to the substantial problem in the same hierarchy. This principle is strictly applied to the Hong's classification system. Second, on the basis of democratic thought, he del·eloped the classification system. In other words, when he set up the priority of classes, he put emphasis on the democratism as a guideline. The organization of classes belong to the catagories of history (Sa-bu, 사부) and philosophy(Cha-bu, 자부) showed the application of this principle. Conclusively, this paper found that Hong did not randomly arrange the class older, but he set the class order with objective reasons and logic when he set the class order of arrangement.