• 대한바이러스학회지
• /
• 제30권2호
• /
• pp.125-130
• /
• 2000

Infection with human cytomegalovirus (HCMV) is of considerable clinical relevance after placental transmission and in immunosuppressed patients such as transplant recipients or patients with AIDS. The rapid detection method of HCMV has been required to overcome the time-consuming methods such as classical plaque assay or other immunological methods. This study was performed to establish the in situ ELISA, in which human lung fibroblasts infected with HCMV were fixed and used directly as antigen in 96 well culture plate. Expressed HCMV antigens were detected with HCMV-specific monoclonal antibodies. This method could detect HCMV dose-dependently upto $3{\times}10^2\;pfu/ml$. Antiviral activity of ganciclovir could be assayed within the known range of effective dose. This result showed that HCMV could be quantitated by in situ ELISA. The chemical, which was selected on the basis of component analysis in natural product, was tested to have the anti-HCMV activity by in situ ELISA, and three among five samples were found to have anti-HCMV activity with the dose-dependent manner. Conclusively in situ ELISA could be useful method for quantitation of HCMV and screening antiviral activity of samples to HCMV.

• 대기
• /
• 제32권3호
• /
• pp.247-261
• /
• 2022

The importance of ozone monitoring has been growing due to the polar ozone depletion and increasing tropospheric ozone concentration over many Asian countries, including South Korea. In-situ measurement of the vertical ozone structure has advantages for ozone research, but observations are not sufficient. In this study, ozonesonde measurements were performed from October to November in Yongin during the GMAP (The GEMS Map of Air Pollution) 2021 campaign. The procedure for ozonesonde preparation and initial analysis of the observed ozone profile are documented. The observed ozone concentrations are in good agreement with previous studies in the troposphere, and they capture the stratospheric ozone distribution as well, including stratosphere-troposphere exchange event. These balloon-borne in situ measurements can contribute to the evaluation of remote sensing measurements such as Geostationary Environment Monitoring Spectrometer (GEMS). This document focuses on providing essential information of ozonesonde preparation and measurement for domestic researchers.

• 멤브레인
• /
• 제26권2호
• /
• pp.141-145
• /
• 2016

고분자전해질막은 전극 이외에 전기 화학 연료전지의 성능을 결정하는 중요한 요소이다. 고분자전해질막은 가스나 양성자 등의 작은 분자를 선택적으로 수송해야 한다. 고분자전해질막을 투과한 가스는 급속히 전기 화학적 환원을 발생시켜 음극 촉매의 열화를 유발하기 때문에 수소 장벽으로 작동해야 하며 가능한 한 빨리 양성자를 이동시켜야 한다. 지금까지 고분자전해질막의 수소 기체 투과도를 측정하는데 한정된 방법(예 : Constant volume/variable pressure (Time-lag)법)을 사용했다. 그러나 측정의 대부분은 고분자전해질막은 건조된 진공 하에서 이루어진다. 그렇지 않으면 얻어진 수소 투과도는 측정 오차가 커지는 원인이 되기 쉽다. 이 연구에서는 일반적으로 고분자전해질막으로 사용되는 Nafion212의 수소 가스 투과 특성을 온도와 습도가 동시에 제어되는 in-situ 측정 시스템을 이용하여 평가하였다.

• 대한기계학회논문집A
• /
• 제21권7호
• /
• pp.1156-1165
• /
• 1997

Complex modal testing is employed for the in-situ parameter identification of a four-axis active magnetic bearing system while the system is in operation. In the test, magnetic bearings are used as exciters as well as actuators for feedback control. The experimental results show that the directional frequency response function, which is properly defined in the complex domain, is a powerful tool for identification of bearing as well as modal parameters. It is also shown that the position and current stiffnesses can be accurately estimated using the relations between the measured forces, displacements, and currents.

• Environmental Engineering Research
• /
• 제10권2호
• /
• pp.71-78
• /
• 2005

A series of column experiments was conducted to develop a monitoring system for in-situ and realtime measurement of ozone transport in unsaturated porous media using a fiber optic sensor. The calibration of the fiber optic transflection dip probe (FOTDP) system was successfully carried out at various ozone concentrations using a column with length of 30 cm and diameter of 5 cm packed with glass beads, which don't react with gaseous ozone. The breakthrough curves (BTCs) of ozone were obtained by converting the normalized intensity into ozone concentration. The FOTDP system worked well for in-situ monitoring of gas phase ozone at various water saturations and in presence of soil organic matter (SOM). However, the FOTDP system did not measure the ozone concentration at more than 70% water saturation.

• 한국광학회지
• /
• 제4권3호
• /
• pp.260-265
• /
• 1993

실시간(in-situ) 스트레스측정 간섭계를 사용하여 이온보조증착에 의해 Ti$O_2$ 박막이 성장되는 동안에 박막내부의 스트레스를 측정하였다. 박막두께에 따른 스트레스의 변화는 이온빔에 의한 압축스트레스와 표면온도의 의해 결정되는 표면확산의 평형에 의해서 정성적으로 설병되어지며, 측정결과는 스트레스가 성장되는 박막에 전달되는 이온빔의 운동량에 따라 증가한다는 Windischmann의 모델과 일치하였다.

• 한국터널지하공간학회 논문집
• /
• 제3권1호
• /
• pp.51-62
• /
• 2001

AE와 DRA를 이용한 초기응력 측정법은 실험실 시험을 통해 초기응력을 측정하는 방법 중의 하나이다. 이 방법은 시추와 실험실 시험간에 지연시간이 발생하며 시료에 축방향 반복하중을 가하여 초기응력을 결정하기 때문에 지하 암반의 응력 상태와는 다른 결과를 얻을 수도 있다. 이 연구에서는 지연시간을 다르게 하여 지연시간이 선행응력 결정에 미치는 영향을 살펴보았다. 그리고 축방향 선행응력만을 가한 시료와 축방향 선행응력과 봉압을 모두 가한 시료에 대해 AE와 DRA를 이용하여 선행응력을 결정하여 봉압이 축방향 선행응력 결정에 미치는 영향을 살펴보았다. 지연시간이 길어질수록 오차가 약간씩 증가하는 경향이 나타났으나 지연시간이 1개월인 시료라 할지라도 AE와 DRA를 이용한 경우 각각 16%와 12%의 오차범위 내에서 선행응력을 결정할 수 있었다. 축방향 선행응력만을 가한 경우 AE를 이용하면 9%, DRA를 이용하면 4%의 오차범위 내에서 축방향 선행응력을 결정할 수 있었으나 축방향 선행응력과 봉압을 모두 가한 경우는 각각 17%와 14%의 오차범위 내에서 축방향 선행응력을 결정할 수 있었다. 터널현장에서 시추한 코어를 이용하여 초기응력을 측정한 결과 AE와 DRA는 유사한 결과를 나타내었으나 수압파쇄법에 의한 결과보다는 작은 값을 갖는 것으로 나타났다.

• 한국철도학회:학술대회논문집
• /
• 한국철도학회 2001년도 춘계학술대회 논문집
• /
• pp.443-450
• /
• 2001

This paper shows the reduction effect of tile earthquake-induced liquefaction potential of soils that improved by Vibro-Replacement Stone Columns. The Vibro-Replacement Stone Columns method transforms soft cohesive soils into a composite mass of compacted granular or crushed stone columns by using vibrating equipment and water jets. This study investigated and analyzed the behavior of the stone columns and composite ground using the results of in situ test and measurement at the job-site. This paper shows the evaluation of the earthquake-induced liquefaction potential of soils using in situ test. There are different types of in situ test used in the evaluation the liquefaction potential. In the particular study the Standard penetration test, and Cone penetration test were used. The N value of Standard Penetration test has been used all over for a very long time. The evaluation of the liquefaction of soil was performed using the worldwide renewed Cone penetration test

• 한국자기학회:학술대회 개요집
• /
• 한국자기학회 1997년도 추계연구발표회 논문개요집
• /
• pp.106-107
• /
• 1997

• 한국공작기계학회:학술대회논문집
• /
• 한국공작기계학회 1999년도 추계학술대회 논문집 - 한국공작기계학회
• /
• pp.93-98
• /
• 1999

Non-contacting capacitive sensors, based on principle of the cross capacitor, for measuring small displacement less than 1.95$\pm$0.5 mm have been fabricated and characterized. To overcome disadvantages of the existed capacitive sensors, the new sensor is consisted of 4-electrodes which are formed 2 active electrodes and 2 guard electrodes on a sapphire plate with diameter 17mm and thickness 0.7 mm, and are symmetrically situated with constant gap of 0.2 mm among the electrodes. The sensor is evaluated to be correlation coefficient of 0.9987 for the range of 1.95$\pm$0.5 mm and that of 0.9995 for 1.95$\pm$0.25 mm range. This sensor can be used for in situ measurements in the mechanical mirror polishing with precision less than $\pm$1${\mu}{\textrm}{m}$.