• 생명과학회지
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• 제17권3호통권83호
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• pp.345-349
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• 2007

PDZ 도메인을 통하여 여러 단백질과 상호작용하며 신경전달 기전에 관여하는 단백질로 Shank1, Shank2, Shank3, PDS-95, AF있다. 본 연구는 Shank3 PDZ 도메인의 구조를 밝히기 위한 첫 단계로서 Shank3 단백질의 PDZ 도메인을 동정하였고, E. coli에서 발현하여 생성된 단백질을 정제한 후 1차 NMR 구조분석을 시도하였다. 그 결과에 의하면 정제된 Shank3 PDZ 단백질은 순도가 높고 안정적인 접힘(folding)구조를 제시하고있다. 현재 완전한 NMR 구조분석을 위해 좀더 많은 양의 정제된 Shank3 PDZ 단백질을 얻고자 연구하고 있다.

• 한국재난정보학회 논문집
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• 제8권3호
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• pp.297-303
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• 2012

본 연구는 목조문화재 지붕 적심부의 화재전파 차단기능을 확보하기 위해 개판 상부에 설치하는 방염천의 차열성능을 알아보기 위한 실물모형 실험을 실시하여 방염천의 차열성능을 검증하고, 그 밖에 방염천의 설치방식에 따른(평형식과 접이식) 방화성능을 비교 분석하였다. 실험 결과, 방염천을 개판 상부에 평형식으로 설치한 경우 개판 하부온도가 $750^{\circ}C$일때 개판 상부온도가 $50^{\circ}C$로 $700^{\circ}C$ 정도의 온도차를 나타냈고 이는 방염천 미설치 시와 비교했을 때 개판 상부온도가 $580^{\circ}C{\sim}600^{\circ}C$ 정도 낮은 것으로, 방염천 설치로 인한 차열성능이 우수한 것으로 평가되었다. 방염천을 개판 상부에 접이식으로 설치한 경우 개판 하부온도가 $600^{\circ}C$일 때 개판 상부온도는 $65^{\circ}C$로 약 $530^{\circ}C$ 정도의 온도차를 보여 평형식으로 설치한 경우보다는 차열성능이 약간 떨어지는 것으로 나타났다.

• Clinical and Experimental Reproductive Medicine
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• 제30권2호
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• pp.127-133
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• 2003

Objective: The aim of this study was to investigate the morphological aspects of testicular tissue before and after freezing-thawing by light and transmission electron microscopy. Methods: Tissue biopsies were carried out on mouse testis for freezing. Samples in medium containing 20% glycerol were frozen by computer-controlled freezing program. The effect of freezing-thawing on the structural change of testicular tissues were examined by light and electron microscopy. Results: The freezing-thawing procedure had no significant effect on tubular diameter. However, it caused folding of the lamina propria, and notable damage to Sertoli cells, spermatogonia and spermatocytes. The cells were detached, desquamated from the basal lamina and had increased vacuolization. Round spermatids, elongated spermatids and spermatozoa were less affected, and most of them maintained their normal structure. Conclusions: The structure of spermatogonia, spermatocyte and basal compartments in seminiferous epithelium was significantly altered by freezing-thawing procedure of mouse testicular tissues. Thus, we need to develop a more reliable method for the cryopreservation of testicular tissues.

• 한국미생물생명공학회:학술대회논문집
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• 한국미생물생명공학회 2001년도 Proceedings of 2001 International Symposium
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• pp.149-153
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• 2001

To investigate the mode of bactericidal action for antimicrobial peptide, pediocin, synthetic and mutant pediocins were prepared by direct chemical synthesis. Native pediocin was purified from Pedio-coccus acidilactici M and its conformational structure and bactericidal functions were analyzed and compared to synthetic pediocin. Schematic mode of pediocin actions, how pediocin binds on the target cell membrane, penetrates and makes tunnel are proposed. For these purposes, primary and secondary structures of pediocin was analyzed and disulfide bond assignment was also done. The pediocin purified from P. acidilactici M had high effective bactericidal ability against gram positive bacteria, especially Listeria monocytogenes and was very stable at extreme pHs and even at high temperatures such as autoclaving temperature (121$^{\circ}C$). Pediocin was consisted of 44 amino acids with four cysteines. Novel synthetic peptides were achieved by solid phase peptide synthesis(SPPS) method. To explain the function of cysteine in C-terminal region, mutant pediocin, Ped[C24A＋C44A], was synthesized and their structural and biological functions were analyzed. Second mutant pediocin, Ped[KllE], was prepared to explain the function of lysine at 11 of N-terminal part of pediocin, especially loop of $\beta$-sheet, and to predict the initial binding site of pediocin. The native and synthetic pediocins was showed random coil conformation by spectropolarimetry in moderate conditions. This conformation was observed in extreme conditions such as high temperature and low and high pHs, also. Circular dichroism(CD) data also showed the existence of $\beta$-turn structure in N-terminal part both native and synthetic pediocins. A structural model for pediocin predicts that 18 amino acids in the N-terminal part of the peptide assume a three-strand $\beta$-sheet conformation. This random coil in C-terminal part of pediocin was converted to folding structure, helix structure, in nonpolar solvents such as alcohol and TFE. The disulfide bond between $^{9}$ Cys and $^{14}$ Cys was concrete and inevitable, however, evidences of disulfide bond between $^{24}$ Cys and $^{44}$ Cys was not. Data of Ped[C24A＋C44A], pediocin mutant showed that $^{44}$ Cys was required during killing the target cells but not inevitable, since Ped[C24A＋C44A] still have bactericidal activity but much less than native pediocin. Another pediocin mutant, Ped[KllE], had still bactericidal activity, was controversial to propose that positive charge like as $^{11}$ Lys in loop or hinge in bacteriocin bound or helped to binding to microorganism with electrostatic interaction between cell membrane especially teichoic acid and positive amino acid nonspecifically. The conformation of pediocin among native, synthetic and mutant pediocins did not show big difference. The conformations between oxidized and reduced pediocin were almost similar regardless of native or synthetic.

• 한국지구과학회지
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• 제42권5호
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• pp.479-495
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• 2021

본 연구는 2020년 3월 18일부터 20일까지 영동지역에 강풍이 발생했던 사례(남고북저형, 대류권계면 접힘에 의해 급격하게 발달하는 저기압)의 종관 및 열역학적/운동학적특성을 조사하기 위해 AWS 관측 자료, 종관 일기도, ECMWF 재분석 자료, 레윈존데, 윈드프로파일러 자료를 이용하였다. 분석결과, 사례 기간 영동지역 5개소에서 관측된 최대 순간 풍속은 20 m s^-1 이상으로 나타났으며 대관령(27.7 m s^-1)에서 가장 강하게 나타났다. 종관분석에서는 남고북저형의 기압배치와 함께 영동지역으로 등압선의 모양이 사인(sin)파 형태를 보이며 강한 기압경도력에 의해 강풍이 발달하다가 3월 19일부터는 한반도 북부지역에서 하루 내에 19 hPa 이상의 기압 하강과 함께 발달하는 저기압에 의해 지속적인 강풍이 발달했다. 북강릉 단열선도에서 역전층의 고도는 하층 강풍대와 함께 산 정상의 약 1-3 km 고도에 위치하였고, 레윈존데 및 수직 측풍 장비(윈드프로파일러)의 연직 바람장 분석 결과와 일치함을 확인할 수 있었다. 특히 열역학적 및 운동학적 연직 분석에서, 하층에서 온위의 연직 경도에 의한 강한 바람과 대류권계면 접힘에 의한 위치 소용돌이도의 발달이 영동지역 강풍 발생에 큰 역할을 한 것으로 사료된다.

• 자원환경지질
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• 제6권2호
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• pp.81-114
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• 1973

The purpose of the present study is to clarify the stratigraphy and geologic structure of the Great Limestone Series by means of study on fossil conodonts and detail investigation of geologic structure. In recent years very few geologists in Korea argue without confident evidences against the age and stratigraphy of the Great Limestone Series which have been rather well established previously in most parts of the regions although it is ambiguous and has not been studied in other areas. Five type localities in the Kangweon basin where the Great Limestone Series is well cropped out were chosen for the study. Total 26 genus and 66 species of conodont were identified from 290 samples collected and treated. From the study on conodonts the age of each formations of the Great Limestone Series has been determined as follows: The Great Limestone Series of Duwibong type Duwibong limestone: Caradocian (mid-Ord.) Jikunsan shale: Landeilian (mid-Ord.) Maggol limestone: Llanvirn-Llandeilian (mid-Ord.) Dumugol: Arenigian (Ord.) Hwajeol: Upper Cambrian The Great Limestone Series of Yeongweol type Mungok (Samtaesan) : Ordovician Machari: upper Cambrian The Great Limestone Series of Jeongseon type Erstwhile Jeongseon limestone: mid-Ord. The erstwhile Jongseon Limestone formation in Jeongseon district is separated into Hwajeol, Dongjeom, Dumudong, and Maggol formations which were cropped out repeatedly by folding and faulting, but Maggol is predominant in areal distribution. Yemi Limestone Breccia bed is not a single bed but distributed in several horizons so that it bears no stratigraphic significance. The limestone bed above Yemi Limestone Breccia, which was believed by some geologists to be much younger than Ordovician, is identified to be Maggol and its age is determined to be mid-Ordovician. Sambangsan formation in Yeongweol district was believed to be Cambrian age and lower horizon than Machari formation by Kobayashi, but C. M. Son believed that it might belong to later than Ordovician and lies above the Great Limestone Series of Yeongweol type. It was identified to be upper Cambrian and lies beneath the Machari formation and above the Daeki formation, the lower most horizon of the Great Limestone Series. The age of Yeongweol type Choseon system is contemporaneous with that of Duwibong type Choseon system. The difference in lithofacies is not due to lateral facies change, but due to the difference in its depositional environment. The Yeongweol type Choseon system is believed to be deposited in the small Yeongweol basin which was separated from the main Kangweon sedimentary basin. Judging from these facts it is definitely concluded that there exists no Gotlandian formation in the regions studied. Structurally the Kangweon basin comprises five basins and two uplifted areas. These structures were originated by at least two crustal movements, that is, Songrim disturbance of Triassic and Daebo orogeny of Jurasic age.

• Journal of Microbiology
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• 제44권1호
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• pp.64-71
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• 2006

To investigate the effects of the nucleotide sequences in Shine-Dalgarno (SD) and the spacer region (SD-ATG) on bovine growth hormone (bGH) gene expression, the expression vectors under the control of the T7 promoter (pT7-7 vector) were constructed using bGH derivatives (bGH1 & bGH14) which have different 5'-coding regions and were induced in E. coli BL21 (DE3). Oligonucleotides containing random SD sequences and a spacer region were chemically synthesized and the distance between the SD region and the initiation codon were fixed to nine bases in length. The oligonucleotides were annealed and fused to the bGH1 and bGH14 cDNA, respectively. When the bGH gene was induced with IPTG in E. coli BL21(DE3), some clones containing only bGH14 cDNA produced considerable levels of bGH in the range of $6.9\%\;to\;8.5\%$ of total cell proteins by SDS-PAGE and Western blot. Otherwise, the bGH was not detected in any clones with bGH1 cDNA. Accordingly, the nucleotide sequences of SD and the spacer region affect on bGH expression indicates that the sequences sufficiently destabilize the mRNA secondary structure of the bGH14 gene. When the free energy was calculated from the transcription initiation site to the +51 nucleotide of bGH cDNA using a program of nucleic acid folding and hybridization prediction, the constructs with values below -26.3 kcal/mole (toward minus direction) were not expressed. The constructs with the original sequence of bGH cDNA also did not show any expression, regardless of the free energy values. Thus, the disruption of the mRNA secondary structure may be a major factor regulating bGH expression in the translation initiation process. Accordingly, the first stem-loop among two secondary structures present in the 5'-end region of the bGH gene should be disrupted for the effective expression of bGH.

• 보존과학연구
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• 통권24호
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• pp.187-213
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• 2003

Sungseonsa Temple site in Chungju city in Chungcheongbuk-doProvince is written in "Goryeosa" as a building for Queen Sinmyeongsunseong, the mother of Gwangjong in AD 954 in Goryeo Dynasty. The museum in Chungcheong University takes charge of the excavation for 3 times from 2000 to 2002 and identified that its construction was carried out till Joseon Dynasty. Among the iron artifacts from the first excavation such as a weeding hoe, a hand knife, a lock, two nails and a plow which had conservation treatments, the sample was collected. Its micro-structure and method of manufacture were investigated. Excavation report for those artifacts has not published yet, therefore, the date of each artifacts is not clearly confirmed. The samples were collected from each part of the objects and then embedded in epoxy resin and etched with nitric acid. The examination of its microstructure is carried out under the microscope and the hardness values were measured by Vickers hardness tester. From the results, some artifacts show different manufacture method sin the each parts. The forming processes of the iron weeding hoe and the iron sickle are similar but the blade of iron weeding hoe was strengthened by carbonization whereas the blade of the iron sickle was done by quenching. The hand knife and the nails were produced through almost same methods and shows similar microstructures. The hand knife seems to be made by repeated beating and folding in low temperature resulting in fine crystallization, but the nail shows large crystallization due to processes in high temperature. Lock is made of white cast iron, that does not show any heat treatment.

• BMB Reports
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• 제37권5호
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• pp.586-596
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• 2004

The folding of ervatamin C was investigated in the presence of various fluorinated and non-fluorinated organic solvents. The differences in the unfolding of the protein in the presence of various organic solvents and the stabilities of O-states were interpreted. At pH 2.0, non-fluorinated alkyl alcohols induced a switch from the native $\alpha$-helix to a $\beta$-sheet, contrary to the $\beta$-sheet to $\alpha$-helix conversion observed for many proteins. The magnitude of ellipticity at 215 nm, used as a measure of $\beta$-content, was found to be dependent on the concentration of the alcohol. Under similar conditions of pH, fluorinated alcohol enhanced the intrinsic a-helicity of the protein molecule, whereas the addition of acetonitrile reduced the helical content. Ervatamin C exhibited high stability towards GuHCl induced unfolding in different O-states. Whereas the thermal unfolding of O-states was non-cooperative, contrary to the cooperativity seen in the absence of the organic solvents under similar conditions. Moreover, the differential scanning calorimetry endotherms of the protein acquired at pH 2.0 were deconvoluted into two distinct peaks, suggesting two cooperative transitions. With increase in pH, the shape of the thermogram changed markedly to exhibit a major and a minor transition. The appearance of two distinct peaks in the DSC together with the non-cooperative thermal transition of the protein in O-states indicates that the molecular structure of ervatamin C consists of two domains with different stabilities.

• 동아시아식생활학회지
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• 제18권1호
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• pp.80-86
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• 2008

Both hand-made and machine-made Chinese noodles are popular in Korea. In this study, each type was evaluated in terms of its physical properties to rigorously determine for consumers which one has better qualities. The noodles were instrumentally measured for color, size, moisture content, density, viscoelasticity, and cutting force. The behaviors of the noodles were visually observed during cooking, and sensory evaluations were performed with the cooked noodles. The hand-made raw noodles were less dense, had higher moisture content, and generated more bubbles during cooking than the machine-made noodles. This indicated that the hand-made noodles contained more entrapped air, thereby resulting in the above physical characteristics. The change in noodle size after cooking was greater in the hand-made noodles, indicating that more entrapped air in expansion escaped during cooking and was replaced by water. The cutting force and viscoelasticity of the hand-made noodles were lower, and were controlled by viscous properties, respectively. These results agreed with the fact that the hand-made noodles had higher moisture content and lower density. In the sensory evaluation, the hand-made noodles presented lower hardness, but higher elasticity. It was inferred that the hand-made noodle dough underwent repeated processes of folding and extending, resulting in better developed of the gluten structure. Consequently, the hand-made noodles were determined to be different than the machine-made noodles in terms of instrumental measurements and sensory observations, suggesting that the hand-made noodles had superior textural properties.