• BMB Reports
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• v.35 no.5
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• pp.498-507
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• 2002

A gene that encodes a thermostable protease, coined thermicin, has been isolated from Thermoanaerobacter yonseiensis that is expressed and characterized in E. coli.. In order to elucidate the molecular characteristics on thermostability of the enzyme, molecular modeling and mutagenesis technology were applied. In the modeling structure, the structural core, including the active site, was well conserved; whereas, the two loop regions were unique when compared to thermitase. The mutant enzyme with the small loop deleted (D190-I196), based on modeling structural information, showed identical enzyme activity. However, when the large loop was deleted (P233-P244), a little lower $K_m$ and even a lower kcat was found. This indicates that the large loop could influence catalytic activity. However, the unfolding temperature ($T_m$), which was determined by a differential-scanning calorimetry for the mutant enzyme deleted the small loop, was $96^{\circ}C$. This is $14^{\circ}C$ lower than that for the parent thermicin. These results suggest that the small loop may play a role in maintaining the proper folding of the enzyme at high temperatures, whereas the large loop might be related to catalysis.

• Korean Institute of Interior Design Journal
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• v.14 no.5 s.52
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• pp.44-51
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• 2005

This study focuses 'Korean characteristics' in architectural interior design. In this study, some Korean characteristics was extracted by analysing traditional Korean spaces and as well as by considering the architectural interior spaces which are designed with Korean character since 1970. This paper started with differenciating the elements and principles that consist of space, and then classified the elements into 8 categories such as Spatial system, Enclosure system, Circulation system, Material system, Furnishing system, Light, Color, Context which are analysing frames. As a conclusion, the concepts of Korean characteristics are extracted and suggested as design concept with Korean characteristics by analysing the contents of each category. These concepts were presented with simple diagrams and supporting cases were also presented in this paper. The design concepts of 'Korean characteristics' are classified in the 25 concepts: Holonic composition, Transitional space, Hierarchy by floor level, Gradation of privacy, Changeability, Various central yards, Space with opened edges, Gray space, Continuity of space, Translucent wall, Folding-lifting wall, Skeleton, Abstract facet, Path as a message, Mild light, Multii-layered vista, Fluidity, framed scenery, Serial view, Interpenetrating Space, Harmony with nature, Organic interrelationship, Complementary Dual Structure, Temporal change of space, Hierarchical order.

• BMB Reports
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• v.35 no.4
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• pp.364-370
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• 2002

The effect of sodium n-dodecyl sulphate (SDS) on hemoglobin autoxidation was studied in the presence of a 100mM phosphate buffer (pH 7.0) by different methods. These included spectorphotometry, fluorescence technique, cyclic voltametry, differential scanning calorimetry, and densitometry. Spectroscopic studies showed that SDS concentrations up to 1 mM increased deoxy-, decreases oxy-, and had no significant effect on the met- conformation of hemoglobin. Therefore, a SDS concentration up to 1 mM increased the deoxy form of hemoglobin as the folded, compact state and decreases the oxy conformation. The turbidity measurements and differential scanning calorimetry techniques indicated a more stable conformation for hemoglobin in the presence of SDS up to 1mM. Electrochemical studies also confirmed a more difficult oxidation under these conditions. The induction of the deoxy form in the presence of SDS was confirmed by densitometry techniques. The compact structure of deoxyhemoglobin blocks the formation of met-conformation in low SDS concentrations.

• 보존과학연구
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• s.13
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• pp.37-58
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• 1992

Iron artifacts from Busǒ Sansǒng inffered to late Baikjae periodwere studied on the aspects of metallugy. These materials were the largest size ever since excavated. From the analytical results these artifacts were found to be pureiron system without impurities or hypo-eutectoid steel system in below 0.3% in carbon contents. From the content of phosphorus in the range of 0.03∼0.05% as aim purity it was shown that charcoal were used for making these iron artifacts from sponge iron not fusion method. By observing metallugical structure it was found that iron artifacts was manufactured by repetitive folding and hammering forging method and some by heating method for adding carbon with cool water. This method were to improve the quality of the soften steel to harden one. In addition to those above repetitive hammering method eliminated the nonferrous materials such as slag inclusion and remained relatively pure ferrite.

• Journal of the Korean Magnetic Resonance Society
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• v.12 no.1
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• pp.1-13
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• 2008

Acetyl-CoA carboxylase (ACC) catalyzes the first step in fatty acid biosynthesis: the synthesis of malonyl-CoA from acetyl-CoA. As essential regulators of fatty acid biosynthesis and metabolism, ACCs are regarded as therapeutic targets for the treatment of metabolic diseases such as obesity, In ACC, the biotinoyl domain performs a critical function by transferring an activated carboxyl group from the biotin carboxylase domain to the carboxyl transferase domain, followed by carboxyl transfer to malonyl-CoA. Despite the intensive research on this enzyme, only the bacterial and yeast ACC structures are currently available, To explore the mechanism of ACC holoenzyme function, we determined the structure of the biotinoyl domain of human ACC2 and analyze its characteristics using NMR spectroscopy. The 3D structure of the hACC2 biotinoyl domain has a similar folding topology to the previously determined domains from E. coli and P. Shermanii, however, the 'thumb' structure is absent in the hACC2 biotinoyl domain. Observations of the NMR signals upon the biotinylation indicate that the biotin group of hACC2 does not affect the structure of the biotinoyl domain, while the biotin group for E. coli ACC interacts directly with the thumb residues that are not present in the hACC2 structure. These results imply that, in the E. coli ACC reaction, the biotin moiety carrying the carboxyl group from BC to CT can pause at the thumb of the BCCP domain. The human biotinoyl domain, however, lacks the thumb structure and does not have additional non-covalent interactions with the biotin moiety; thus, the flexible motion of the biotinylated lysine residue must underlie the "swinging arm" motion. This study provides insight into the mechanism of ACC holoenzyme function and supports the "swinging arm" model in human ACCs.

• The Journal of the Petrological Society of Korea
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• v.10 no.2
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• pp.121-131
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• 2001

The combination of geological, structural and satellite image studies is used to make an examination of the Miocene eruptive type in the Eoil Basin, SE Korea. The basin subsided by the NW-SE extension due to NNW dextral shearing during the East Sea opening. Based on geological structures as well as lithofacies and ages of the basin-fills, it is divided into the NE subbasin and the SW subbasin which were abundantly filled with basaltic volcanics and marine sediments without volcanic materials, respectively: Syndeposional synclines and anticlines are characteristically developed in the NE subbasin, which amplitudes decrease away from the adjacent normal faults to make them into a homoclinal structure. The thicker lavas as well as the younger agglomerates and lacustrine sediments, which show circular distributions, are distributed around the axial zones of major synclines. The satellite image shows four remarkable circular structures within the NE subbasin. They are located adjacent to and along the normal faults, and they are laid almost exactly on the axial zones of the synclines as well as on the distribution area of the agglomerates and lacustrine sediments. These facts indicate that the basaltic lava effusion were conducted by the normal faults like a kind of fissure-eruption and its activity was more predominant at the sites in where the synclines are developed. More active effusion of lava became a reason for deeper subsidence to make differential subsidence and syndepositional folding adjacent to and along the normal faults. Hence, we suggest that a nested cauldron structure was formed in the NE subbasin of the Eoil Basin, and that the volcanism made the subbasin to be a lava pond and controlled the process of filling and sedimentation in the subbasin.

• Bulletin of the Korean Chemical Society
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• v.29 no.10
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• pp.1937-1940
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• 2008

The P1 duplex of Tetrahymena group I ribozyme is the important system for studying the conformational changes in folding of ribozyme. The formation of the P1 duplex between IGS and substrate RNA and the catalytic activity of ribozyme require a variety of metal ions such as $Mg^{2+}$ and $Mn^{2+}$. In order to investigate the effect of the $Mg^{2+}$ concentration on the conformation of the P1 duplex, the NMR study was performed as a function of $Mg^{2+}$ concentration. This study revealed that the less stable AU-rich region formed duplex at $50{^{\circ}C}$ under high $Mg^{2+}$ concentration condition but melts out under low $Mg^{2+}$ concentration condition. It was also found that in the active conformation under 10 mM $MgCl_2$ condition, the unstable central G${\cdot}$U wobble pair maintains the significant base pairing up to $50{^{\circ}C}$. This study provides the information of the unique feature of the P1 duplex structure and the roll of $Mg^{2+}$ ion on the formation of the active conformation.

• KIEAE Journal
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• v.16 no.3
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• pp.35-46
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• 2016

Purpose: This paper aims to analyse the composition system of architectural elements including shape, kinetic and material elements of kinetic facades and establish the design parameter system as a common conceptual and practical knowledge sharing platform with mechanical and electrical experts. Method: This research has been conducted in a three steps. At first, 120 cases of external shading devices are analyzed and their classification criteria have been established. Secondly geometric, kinetic and material elements are categorized in a common kinetic facade coordinates system considering environmental effects and operation method, and the applicability of combination of each element are tested. Lastly core design parameters for each element have been established in a common office building installation coordinate. Result: Geometry elements are categorized into seven geometric shapes and kinetic elements is categorized into basic linear and rotational motion and combinational folding and rolling motion. The combined set of parameters for three elements composes the whole design parameters for architectural elements of kinetic façade. Design parameters of shape elements are composed of shape, installation and arrangement parameters; design parameters for kinetic elements are composed of axis and range parameters; and design parameters of material elements are composed of thermal, lighting and color parameters.

• Journal of the Korean Society of Costume
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• v.30
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• pp.295-306
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• 1996

A large quantity of exhumed clothing found for recent 20 years has becom-e the material for making a concrete clarification of the structure of clothing in the Chosun dynasty. Exhumed clothing means the clothing found in the coffin when the grave of the ancestor buried several hundred several hundred years ago is removed to another pla-ce and it is almost comprised of ordinary clothes of the grave-owner not mourning clothes as well as clothing and ornaments in the period between the 15th cen-tury and th 19th century. This research is intended to inquire into the kind of chimas in the 16th century before the Imjin Japancese Invasion of Korea during 1592-1598. The after effect of the Imjin Japanese In-vasion was generally reflected in clothing them and the structure of clothing in the Chosun dynasty began to overshadew its di-chotomy with the Imjin Japanese I vasion of Korea as the point. Accordingly this research is concerned with the study of the chima system of the early Chosun dynasty which shows that there is a common point of the identical form though clothing was exhumed in different graves. Therefore it was possible to grasp women's clothing that was fashionable at that time The object for research is the women in the upper class of the 16th century whose representative characteristics are depicted as follows: 1. Women in the upper class wore the splendid clothing similar to the ordinary clothing of court ladies. 2. The division of ordinary chima and ceremonial chima appeared in the concrete. 3. Ceremonial chima had its extra width and length and exhibited its exaggerated silhouette. in particular it had a various forms of over-pleats made at its center front which play a role in supplementing discomfort in walking. This associate bustle and crinolin styles through which the modern design of clothing can be glimpsed. 4. The opposite phenomenon was shown that has been perceived up to now such as folding at the right plain seam the treatment of the edge-and the like. 5. The elaboration of manual needlework shows the culmination of women's workmanship.

• BMB Reports
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• v.35 no.5
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• pp.472-475
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• 2002

Phaseolus vulgaris phytohemagglutinin L is a homotetrameric-leucoagglutinating seed lectin. Its three-dimensional structure shows similarity with other members of the legume lectin family. The tetrameric form of this lectin is pH dependent. Gel filtration results showed that the protein exists in its dimeric state at pH 2.5 and as a tetramer at pH 7.2. Contrary to earlier reports on legume lectins that possess canonical dimers, thermal denaturation studies show that the refolding of phytohemagglutinin L at neutral pH is irreversible. Differential scanning calorimetry (DSC) was used to study the denaturation of this lectin as a function of pH that ranged from 2.0 to 3.0. The lectin was found to be extremely thermostable with a transition temperature around $82^{\circ}C$ and above $100^{\circ}C$ at pH 2.5 and 7.2, respectively. The ratio of calorimetric to vant Hoff enthalpy could not be calculated because of its irreversible-folding behavior. However, from the DSC data, it was discovered that the protein remains in its compact-folded state, even at pH 2.3, with the onset of denaturation occurring at $60^{\circ}C$.