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Evaluation on Microbiological Contamination Level of Raw Beef from Retail Markets in Seoul, Korea (서울지역 식육판매점의 우육에 대한 미생물학적 오염도 평가)

  • Ko, Eun-Kyung;Heo, Eun Jeong;Kim, Young Jo;Park, Hyun Jung;Wi, Seong-Hwan;Moon, Jin San
    • Food Science of Animal Resources
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    • v.33 no.3
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    • pp.403-410
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    • 2013
  • This study was performed to evaluate the microbiological contamination level of raw beef from retail markets in Seoul, Korea. The sampling and laboratory test were performed according to the procedure of "Standard for processing and ingredients specification of livestock product" and "Korean food code". Enterotoxin of Staphylococcus aureus isolates were detected using VIDAS$^{(R)}$ and PCR-based methods. Listeria monocytogenes serotyping and genotyping were carried out using Listeria antisera and L. monocytogenes Fingerprinting kit, respectively. A total of 48 samples were collected from 16 retail markets (butcher's shop: 5, department store: 6, supermarket: 5) in 2011. The level of total bacteria counts in the butcher's shop, department store and supermarket were $4.4{\times}10^3$ CFU/g, $3.9{\times}10^5$ CFU/g and $1.0{\times}10^4$ CFU/g, respectively. The concentrations of Escherichia coli of these three retail markets were $6.4{\times}10$ CFU/g, 7.6 CFU/g and $2.0{\times}10$ CFU/g, respectively. Salmonella species was not detected on all samples. However, S. aureus was isolated in the 3 samples (6.25%) from each type of three retail markets. L. monocytogenes was isolated in the 4 samples (8.3%) from department stores. The level of contamination of these foodborne bacteria was less than 100 CFU/g. The enterotoxin-encoding genes of S. aureus isolates were sea, seh, sei and sep gene. The gene similarity of L. monocytogenes isolated from two retail markets by Rep-PCR showed 57.8-98.1% and 68.1-98.1%, respectively. These results suggest that the HACCP guideline for environmental control in slaughterhouse and retail markets should be provided to prevent cross contamination and manage foodborne pathogens such as L. monocytogenes and S. aureus.

Characteristic of mycelial growth of cauliflower mushroom (Sparassis latifolia) using replacement culture with Trichoderma and rDNA analysis in genealogy of crossbreeding strain (푸른곰팡이 대치배양에 의한 꽃송이버섯 균사 생장 특성 및 계통간 교잡균주의 rDNA 분석)

  • Oh, Deuk-Sil;Kim, Hyun-Suk;Kim, Young;Wi, An-Jin;Yoon, Byung-Sun;Park, Whoa-Shig;Park, Hyeong-Ho;Wang, Seung-Jin
    • Journal of Mushroom
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    • v.12 no.1
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    • pp.41-51
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    • 2014
  • Cauliflower mushroom widely known high concent of ${\beta}$-glucan for farm cultivation invigoration verified characteristics of mycelia growth, genetic diversity, resistance to Trichoderma by replacement culture with Trichoderma and growth characteristics of new variety crossbleeding strain. The result of replacement culture with Trichoderma for verification resistance about Trichoderma, 6951 (T. viride) strain did not show special change after formation of confrontation line and 6952 (T. spp.) strain was showed more formation of spore after formation of confrontation line. But 6426 (T. harzianum) strain found to encroach part of growth area of cauliflower mushroom mycelia. Among 10 kinds cauliflower mushroom strain, JF02-06 strain collected by Gurye, found did not spore of Trichoderma and thought to be resistant to Trichoderma. The result of crossbleeding after selected that mother strain good growth and formation of fruit body, verified good mycelia growth at JF02-47, 49 and 50 strain in Korean pine of wood-chip media. The result of gene sequence about ITS1, 5.8S and ITS4 for analysis of genetic diversity at crossbleeding strain, found high significance to other cauliflower mushroom in registered Genebank. The result of growth characteristic of spore and mycelia of cauliflower mushroom by observation microscope, size of spore showed water drop shape to major axis $6{\mu}m$ and minor axis $5{\mu}m$ and clamp showed 3 types in mycelia. The wide of mycelia was $3{\mu}m$. The characteristic of mycelia of cauliflower mushroom found to grow mycelia in clamp at approximately 50%. The growth speed of mycelia was $0.507{\mu}m/min$ and 2nd mycelia grown similar speed to mother mycelia at parallel with mother mycelia after growth speed at $0.082{\mu}m/min$. The formation of clamp made small clamp for 5 hours after shown transfer of electrolyte in mycelia inside. The septum formation started after 3 hours and then finally completed after 2 hours. In this study, strain of cauliflower mushroom verified resistance of Trichoderma, genetic diversity and characteristic of mycelia growth. Therefore, basic knowledge of cauliflower mushroom will improve and further contribute to development of mushroom industry.

Impact of Elevating Temperature Based on Climate Change Scenarios on Growth and Fruit Quality of Red Pepper (Capsicum annuum L.) (기후변화 시나리오에 근거한 온도상승이 고추의 생육양상 및 과실특성에 미치는 영향)

  • Song, Eun Young;Moon, Kyung Hwan;Son, In Chang;Wi, Seung Hwan;Kim, Chun Hwan;Lim, Chan Kyu;Oh, Soonja
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.17 no.3
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    • pp.248-253
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    • 2015
  • This study was conducted to determine the impact of temperature elevated based on climate change scenario on growth and fruit quality of red pepper (Capsicum annuum L.) in walk-in plant growth chambers. The intraday temperatures of climate normal years (IT) were determined using intraday mean temperatures of climatic normal years (1971~2000) in the Andong Province during the growing season (May 1~July 30). Red pepper plants were cultivated under different temperatures (starting at IT rise by up to $6^{\circ}C$, $2^{\circ}C$ increment). Plant height, stem diameter, branch number, leaf number, fresh weight and dry weight increased under the temperatures higher than IT. The number of flower was the greatest under IT+$2^{\circ}C$ (mean temperature at $22.8^{\circ}C$). The total number and the weight of fruits were the highest under IT+$2^{\circ}C$. While the fruit weight, fruit length and fruit diameter decreased more than IT+$2^{\circ}C$ as the temperature increased gradually. These results concluded that in condition that the current diurnal temperature change cycle is maintained in Andong area, in accordance with climate change scenarios, when the temperature rise $2^{\circ}C$ higher than intraday temperature of Andong area the quantity of pepper fruits will increase while maintaining quality, but increases more than that degree yields are expected to decrease significantly. This result suggests that the fruit yield could increase under IT+$2^{\circ}C$ and fruit quality could maintain great, but the fruit yield could decrease under the temperatures higher than IT+$2^{\circ}C$.

A Study on the aesthetic of Calligraphy on Changam, Lee Samman (창암(蒼巖) 이삼만(李三晩)의 서예미학(書藝美學) 고찰)

  • Kim, Doyoung
    • The Journal of the Convergence on Culture Technology
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    • v.6 no.2
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    • pp.99-106
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    • 2020
  • Changam, Lee Samman(1770~1845), who created his own handwriiting to be referred to as the three great writers of the late Joseon Dynasty, invented as the original 'Haengunyusu Typeface' and developed Calligraphy spirit of DonggugJinche in Honam province. He ultimately pursued the state of tonglyeong by raising the personality of 'writing is the person's personality' and the attitude of learning the old. Through the book chang-amseogyeol, he basically polished Haeseo of Han Dynasty and Wi Dynasty and emphasized that Haengseo and Choseo are done automatically when muscle strength and bone strength are established. And since calligraphy originated from 'nature', it goes through the 'Beobcheongwijin' spirit. After doing so, expressed the state of tonglyeong of " mubeob-ibeob ", the stage of reaching. In addition, Changam showed the aesthetic that you can get the novelty by pursuing the philosophy of 'Wu' and the 'beauty of Stupid and Lacking' based on LaoTzu and ChuangTzu. This is a philosophy that follows nature's logic to reveal nature's nature. And it is an aesthetic that protects his 'True Wu' without knowing and greedy. On the other hand, Changam promoted natural and vital beauty through force in the method of using the brush. He suggested the 'Push and Hard' of the Han dynasty, pushing it with force using this power properly. In particular, the feeling of an IlunMujeog brush in 『Changam Calligraphy-The cloud stays Poem』 overflows with the vitality and bizarre and strange dynamism of the spirit and typeface as eum-yang harmonizes with each other. In addition, the beauty of Push and Hard containing polyeoghamse is misaligned, but it has achieved a natural aesthetic without invading. This work demonstrates the real look of Changam choseo. In addition, the beauty of Push and Hard containing polyeoghamse is misaligned, but it has achieved a natural aesthetic without invading. Changam proves the real look of "Haedong's best Chose Maestro".

Photosynthetic and Growth Responses of Chinese Cabbage to Rising Atmospheric CO2 (대기 중 CO2 농도의 상승에 대한 배추의 광합성과 생장 반응)

  • Oh, Soonja;Son, In-Chang;Wi, Seung Hwan;Song, Eun Young;Koh, Seok Chan
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.18 no.4
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    • pp.357-365
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    • 2016
  • The effects of elevated atmospheric $CO_2$ on photosynthesis and growth of Chinese cabbage (Brassica campestris subsp. napus var. pekinensis) were investigated to predict productivity in highland cropping in an environment where $CO_2$ levels are increasing. Vegetative growth, based on fresh weight of the aerial part, and leaf characteristics (number, area, length, and width) of Chinese cabbage grown for 5 weeks, increased significantly under elevated $CO_2$ ($800{\mu}mol{\cdot}mol^{-1}$) compared to ambient $CO_2$ ($400{\mu}mol{\cdot}mol^{-1}$). The photosynthetic rate (A), stomatal conductance ($g_s$), and water use efficiency (WUE) increased, although the transpiration rate (E) decreased, under elevated atmospheric $CO_2$. The photosynthetic light-response parameters, the maximum photosynthetic rate ($A_{max}$) and apparent quantum yield (${\varphi}$), were higher at elevated $CO_2$ than at ambient $CO_2$, while the light compensation point ($Q_{comp}$) was lower at elevated $CO_2$. In particular, the maximum photosynthetic rate ($A_{max}$) was higher at elevated $CO_2$ by 2.2-fold than at ambient $CO_2$. However, the photosynthetic $CO_2$-response parameters such as light respiration rate ($R_p$), maximum Rubisco carboxylation efficiency ($V_{cmax}$), and $CO_2$ compensation point (CCP) were less responsive to elevated $CO_2$ relative to the light-response parameters. The photochemical efficiency parameters ($F_v/F_m$, $F_v/F_o$) of PSII were not significantly affected by elevated $CO_2$, suggesting that elevated atmospheric $CO_2$ will not reduce the photosynthetic efficiency of Chinese cabbage in highland cropping. The optimal temperature for photosynthesis shifted significantly by about $2^{\circ}C$ under elevated $CO_2$. Above the optimal temperature, the photosynthetic rate (A) decreased and the dark respiration rate ($R_d$) increased as the temperature increased. These findings indicate that future increases in $CO_2$ will favor the growth of Chinese cabbage on highland cropping, and its productivity will increase due to the increase in photosynthetic affinity for light rather than $CO_2$.

Evaluation of reverse transcriptase loop-mediated isothermal amplification (RT-LAMP) assay for detection of infectious hematopoietic necrosis virus (IHNV) (Infectious hematopoietic necrosis virus (IHNV)-검출 Reverse transcriptase loop-mediated isothermal amplification (RT-LAMP) 법의 평가)

  • Kim, Wi-Sik;Jeon, Chan-Hyeok;Kim, Jeong-Ho;Oh, Myung-Joo
    • Journal of fish pathology
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    • v.25 no.3
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    • pp.257-262
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    • 2012
  • A reverse transcriptase loop-mediated isothermal amplification (RT-LAMP) assay was evaluated to monitor infectious hematopoietic necrosis virus (IHNV) from artificially infected rainbow trout Oncorhynchus mykiss. The cumulative mortalities of fish challenged with IHNV at $10^{6.5}\;TCID_{50}$/fish, $10^{5.5}\;TCID_{50}$/fish and $10^{4.5}\;TCID_{50}$/fish were 40%, 0% and 0%, respectively. Dead fish and survivors at 16 and 28 d post-challenge in each group were employed for IHNV detection by RT-LAMP assay and virus isolation using BF-2 cells. IHNV from $10^{4.3}$ to $10^{6.8}\;TCID_{50}/ml$ was isolated from all the dead fish and also detected in all of the examined dead fish by RT-LAMP assay. In survivors at 16 d, 60% (3/5 fish, $10^{2.8}-10^{5.05}\;TCID_{50}/ml$), 20% (1/5 fish, $10^{1.05}\;TCID_{50}/ml$) and 60% (3/5 fish, $10^{1.05}-10^{4.8}\;TCID_{50}/ml$) were found to be IHNV-positive by virus isolation in fish challenged with IHNV at $10^{6.5}\;TCID_{50}$/fish, $10^{5.5}\;TCID_{50}$/fish and $10^{4.5}\;TCID_{50}$/fish, respectively, while 20% (1/5 fish), 0% (0/5 fish) and 20% (1/5 fish) were IHNV-positive by RT-LAMP assay. No IHNV was detected in the survivors at 28 d and control fish. These results indicate that the RT-LAMP assay is useful for detection of IHNV in diseased fish although it is not enough to monitor virus in IHNV-survivors.

A study on distinctive view of Cheng I's the sage-theory (정이(程?) 성인론(聖人論)의 특징에 관한 고찰)

  • Kim, Sang-Rae
    • The Journal of Korean Philosophical History
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    • no.56
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    • pp.151-180
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    • 2018
  • Since the completion of the theories on human ethics and moral had been established to pursue by Confucian thinkers like Confucius and Mencius, they generally had agreed to present the basic principles for human education which every human could be the sage. In these principles for human ethics and morality there is on the premise that the knowledge about your own ethical and that the completion of the so-called act(爲) and learning(學). They had given to us that how to get a goal for the ethical and moral lives there are several academic oriented methodology will have act and learning set. In the point of achieving complete figures which act and learning for good society, there was named the sage(聖). This concept sage has two major types. One is on for the political figures that completed, and the other one is for the realm of academic side. Confucian as above mentioned the moral human being is equipped with a complete personality and political ability to make man and society perfect. Confucius has been understood as a complete human being. Yes, ideal for these two types of figures will be fulfilled in some way? They take a mystical ability to a priori or a posteriori, such as human effort can reach the sage. There are many thinkers are obvious and logical answer for this major problem in the system of confucian philosophy I have been trying. About the sage(聖), inherently natural learning(生知) occur to the position sage or knowledge (學知), can lead to there are two of the doctrine for that problem. With the study of learning and knowledge on human beings and real society the two systems concerned together. In fact, the main content of the "Analects of Confucius" we have a set of ethical and moral values not the benevolent conversation about Jin(仁) and his disciples a steady emphasis but on in praise of learning (學) for. However, at the time in Han Tang(漢唐) Han Wi(韓愈) and Wang Chung(王充), according to such thinkers the sage is already a priori determined, cannot be reached by human effort. But At the beginning of the Neo-Confucianism, Cheng I(程?) for the pioneer this Song(宋) scholars, regarding this issue could rebirth the thought that every human could be the sage through the learning as the pre-Chin(先秦) times.

The Effects of Treatment Dumbbell Exercise on Body Composition, Fitness, and Blood Lipid Profiles in Sarcopenic Elderly (미용덤벨 운동이 노인의 근감소증 예방을 위한 신체조성, 체력 및 혈중지질에 미치는 영향)

  • So, Wi-Young;Song, Mi-Soon;Cho, Be-Long;Park, Yeon-Hwan;Kim, Yeon-Soo;Lim, Jae-Young;Kim, Seon-Ho;Song, Wook
    • 한국노년학
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    • v.29 no.3
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    • pp.837-850
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    • 2009
  • Previous epidemiological studies reported that significant muscle loss is observed with advancing aging, called sarcopenia. This study is to investigate the effects of treatment dumbbell exercise on prevention of sarcopenia. The subjects were elderly between 60~70 years old who participated in J-Welfare senior center exercise program at J-gu in S-city and divided into control group(N=19) and exercise group(N=8). Treatment dumbbell exercise was performed 2 times per week for 12 weeks and body composition, fitness, and blood lipid profiles were measured before and after this program. There was no significance in body fat before and after 12 weeks of treatment dumbbell exercise, but there was significance in weight(F=4.312, p=0.048), BMI(F=4.532, p=0.043), and FFM(F=4.743, p=0.039). There was no significance in fitness such as arm curl(F=1.103, p=0.304), and back scratch(F=0.214, p=0.648), but there was significance in 2-minute step(F=33.638, p<0.001), chair stand(F=14.575, p=0.001), chair sit and reach(F=7.198, p=0.013), and 8-ft up and go(F=14.890, p=0.001). The variables of blood lipid profiles such as TC(F=0.030, p=0.864), TG(F=0.142, p=0.710), HDL(F=2.066, p=0.163), glucose (F=0.125, p=0.727), and HbA1c(F=0.945, p=0.340) has no significance. It was found that treatment dumbbell exercise has positive effects on body composition and fitness but has no positive effects on blood lipid profiles of the elderly.

Determining Spatial and Temporal Variations of Surface Particulate Organic Carbon (POC) using in situ Measurements and Remote Sensing Data in the Northeastern Gulf of Mexico during El $Ni\tilde{n}o$ and La $Ni\tilde{n}a$ (현장관측 및 원격탐사 자료를 이용한 북동 멕시코 만에서 El $Ni\tilde{n}o$와 La $Ni\tilde{n}a$ 기간 동안 표층 입자성 유기탄소의 시/공간적 변화 연구)

  • Son, Young-Baek;Gardner, Wilford D.
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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    • v.15 no.2
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    • pp.51-61
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    • 2010
  • Surface particulate organic carbon (POC) concentration was measured in the Northeastern Gulf of Mexico on 9 cruises from November 1997 to August 2000 to investigate the seasonal and spatial variability related to synchronous remote sensing data (Sea-viewing Wide Field-of-view Sensor (SeaWiFS), sea surface temperature (SST), sea surface height anomaly (SSHA), and sea surface wind (SSW)) and recorded river discharge data. Surface POC concentrations have higher values (>100 $mg/m^3$) on the inner shelf and near the Mississippi Delta, and decrease across the shelf and slope. The inter-annual variations of surface POC concentrations are relatively higher during 1997 and 1998 (El Nino) than during 1999 and 2000 (La Nina) in the study area. This phenomenon is directly related to the output of Mississippi River and other major rivers, which associated with global climate change such as ENSO events. Although highest river runoff into the northern Gulf of Mexico Coast occurs in early spring and lowest flow in late summer and fall, wide-range POC plumes are observed during the summer cruises and lower concentrations and narrow dispersion of POC during the spring and fall cruises. During the summer seasons, the river discharge remarkably decreases compared to the spring, but increasing temperature causes strong stratification of the water column and increasing buoyancy in near-surface waters. Low-density plumes containing higher POC concentrations extend out over the shelf and slope with spatial patterns and controlled by the Loop Current and eddies, which dominate offshore circulation. Although river discharge is normal or abnormal during the spring and fall seasons, increasing wind stress and decreasing temperature cause vertical mixing, with higher surface POC concentrations confined to the inner shelf.

Plastic Behaviro of Two Phase Intermetallic Compounds Based on $Li_2$-type$(Ai, Cr)_3$/Ti ($Li_2$$(Ai, Cr)_3$/Ti기 2상 금속간화합물의 소성거동)

  • Park, Jeong-Yong;O, Myeong-Hun;Wi, Dang-Mun;Miura, S.;Mishima, Y.
    • Korean Journal of Materials Research
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    • v.4 no.8
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    • pp.906-914
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    • 1994
  • Plastic behavior of two-phase intermetallic compounds based on $LI_{2}$-type $(Al, Cr)_3$ Ti was investigated using compression test at R.T. and 77K. $LI_{2}$ single phase alloys and two-phase alloys consisting of mainly $LI_{2}$ phase and a few or 20% second phases were selected from AI-Ti-Cr phase diagram. In general, compared with Llz single phase, two-phase alloys consisting of 20% second phase showed relatively high yield strength and poor ductility. Among the alloys, however, AI-21Ti-23Cr alloy consisting of 20% $Cr_{2}Al$ phase showed available ductility as well as high yield strength. Plastic behavior of $LI_{2}$ single phase alloys and two-phase alloys consisting of a few% $Cr_{2}Al$ was also investigated. Homogenization of arc melted ingots substantially reduced the amount of second phases but introduced extensive pore. When Cr content increased in $Ll_{2}$ single phase alloys after the homogenization, the volume fraction of pore in the alloys decreased, and no residual pore was observed in two-phase alloys consisting of a few% $Cr_{2}Al$ phase. Environmental effect on the ductility of the alloys was investigated using compression test at different strain rates($1.2 \times 10^{-4}/s$ and $1.2 \times 10^{-2}/s$). Environmental embrittlement was least significant in A1-25Ti-10Cr alloy consisting of LIZ single phase among the alloys tested in this study. However, based on the combined estimation of the pore formation, environmental embrittlement and ingot cast structure, AI-21Ti-23Cr alloy consisting of 20% $Cr_{2}Al$ as the second phase is expected to show the best tensile elongation behavior.

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