• 생약학회지
• /
• 제35권2호통권137호
• /
• pp.134-138
• /
• 2004

A bioassay-guided fractionation of the whole extract of Viscum album var. coloratum Ohwi (Loranthaceae) led to the isolation of two triterpenoidal components; oleanolic acid (1) and ${\beta}-amyrin$ acetate (2), and a flavonoid, homoflavoyadorinin B (3) as well as large quantity of free fatty acid mixtures as active ingredients of the extract responsible for the antitumoral property. The EtOAc soluble fraction and BuOH soluble fraction of the extract demonstrated a significant inhibition on the proliferation of cultured human tumor cells such as A549 (non small cell lung), SK-OV-3 (ovary), SK-MEL-2 (melanoma), XF498 (central nerve system), and HCT-15 (colon) in vitro, whereas the remaining water soluble fraction exhibited a poor inhibition. The intensive phytochemical investigation of the EtOAc soluble fraction and BuOH soluble fraction of the extract indicated that the oleanolic acid (1) and large amounts of free fatty acid mixtures might be attributed to the in vitro antitumoral property of the whole extract of Viscum album var. coloratum.

• 한국결정성장학회지
• /
• 제26권6호
• /
• pp.243-251
• /
• 2016

실란처리된 하이드록시프로필 메틸셀룰로오스/상전이된 칼슘포스페이트 복합체 시멘트는 향상된 생체적합성을 가지는 주입이 가능한 골시멘트로서 제조 되었다. 복합체 시멘트의 고체상과 액체상으로서 상전이된 칼슘포스페이트와 실란처리된 하이드록시프로필 메틸셀룰로오스의 결정구조 및 화학적 상태는 XRD 및 FT-IR에 의해 측정 되었고, 시멘트의 조작성 및 기계적 물성은 주입성 및 3점 굽힘 시험에 의해 측정하였다. 체외에서 진행된 골시멘트의 기계적 물성, XRD 및 SEM 결과는 골시멘트의 고유 기능으로서의 향상된 경화거동을 보였다. 또한 골시멘트의 세포독성 결과 향상된 생체적합성을 보였다. 따라서 이러한 주입이 가능한 시멘트는 생체의학 응용에 대한 칼슘포스페이트 시멘트로서 사용이 가능하리라 판단되어진다.

• 대한의생명과학회지
• /
• 제3권2호
• /
• pp.71-76
• /
• 1997

실험적 자궁암 치료제로서 본 연구소에서 합성한 마이토센유사체를 인체 자궁암세포주인 SiHa, C33A, HeLa, CaSki에 처리하여, 체외 항암활성 측정시 합성 마이토센유사체들은 이 들 세포주에 대하여 대조 항암제들에 비하여 의의있는 세포독성을 보였으며, SiHa 세포주를 이용한 종양클론형성 억제검사에서는 22번 화합물만이 종양성장 억제활성이 가장 우수하였고 다른 화합물들은 대조항암제들에 비하여 활성이 낮았다. 이 마이토센유사체들에 대한 체외 항암감수성결과는 향후 전임상적인 자궁경부암 화합요법제의 기초자료로 유용할것으로 생각된다.

• 폴리머
• /
• 제35권2호
• /
• pp.119-123
• /
• 2011

본 연구에서는 항암제 전달체로 응용하기 위하여 천연고분자인 히알루론산(hyaluronic acid, HA)에 소수 성기 도입을 위하여 담즙산(bile acid) 중 하나인 디옥시콜산(deoxycholic acid)(DA)을 개질하여 양친성 공중합체를 제조하였고, 이를 항암제 전달체로 응용하고자 하였다. 디옥시콜산이 결합된 히알루론산(HADA)의 물리화학적 특성은 $^1H$ NMR, FTIR, spectrophotometer와 TEM을 이용하여 측정하였다. 디옥시콜산이 결합된 히알루론산에 항암제(파클리탁셀)를 투석방법을 통하여 봉입시켰고, in vitro에서 KB 세포에 대한 항암활성을 확인하였다. 제조된 디옥시콜산이 결합된 히알루론산이 항암제 전달체로서의 응용 가능성을 제시하였다.

• Carbon letters
• /
• 제21권
• /
• pp.61-67
• /
• 2017

We report the use of carrot, a new and inexpensive biomaterial source, for preparing high quality carbon dots (CDs) instead of semi-conductive quantum dots for bioimaging application. The as-derived CDs possessing down and up-conversion photoluminescence features were obtained from carrot juice by commonly used hydrothermal treatment. The corresponding physiochemical and optical properties were investigated by electron microscopy, fluorescent spectrometry, and other spectroscopic methods. The surfaces of obtained CDs were highly covered with hydroxyl groups and nitrogen groups without further modification. The quantum yield of as-obtained CDs was as high as 5.16%. The cell viability of HaCaT cells against a purified CD aqueous solution was higher than 85% even at higher concentration ($700{\mu}g\;mL^{-1}$) after 24 h incubation. Finally, CD cultured cells exhibited distinguished blue, green, and red colors, respectively, during in vitro imaging when excited by three wavelength lasers under a confocal microscope. Offering excellent optical properties, biocompatibility, low cytotoxicity, and good cellular imaging capability, the carrot juice derived CDs are a promising candidate for biomedical applications.

• Bulletin of the Korean Chemical Society
• /
• 제30권2호
• /
• pp.435-440
• /
• 2009

19 Aromatic ring and L-amino acid ester contained rupestonic acid amide derivatives 2a~2l, 3a~3g were synthesized and preliminarily evaluated in vitro against influenza virus $A_3$,B and herpes simplex virus type 1 (HSV-1), 2(HSV-2) by the national center for drug screening of China. The rusults showed that 2i possessed the highest inhibition against both influenza virus $A_3\;(TC_{50}\;=\;120.6\;{\mu}mol/L,\;IC_{50}=\;19.2\;{\mu}$mol/L, SI = 6.3) and B (T$C_{50}\;=\;120.6\;{\mu}mol/L,\;IC_{50}=\;29.9\;{\mu}$mol/L, SI = 4.0); 2g was more active against influenza $A_3$ virus at very low cytotoxicity ($TC_{50}\;>\;2092.1\;{\mu}mol/L,\;IC_{50}=\;143.7\;{\mu}mol/L,$ SI > 14.6) than the parent compound; Compounds 2b, 2c, 2f showed higher activities both against HSV-1 and HSV-2 than that of the parent compound, and 2f was the most potent inhibitor of HSV-1 ($TC_{50}\;=\;200.0\;{\mu}mol/L,\;IC_{50}\;=\;11.3\;{\mu}mol$/L, SI = 17.7 ) and HSV-2 ($TC_{50}\;=\;200.0\;{\mu}mol/L,\;IC_{50}\;=\;20.7\;{\mu}mol$/L , SI = 9.7).

• 대한한의학회지
• /
• 제20권2호
• /
• pp.128-140
• /
• 1999

To evaluate the antitumor activity, antimetastatic and immunomodulatory effects of samryongbakchulsankamibang(SBSK) studies were done experimentally, In cytotoxicity against P388, A549. SK-OV-3, B16-F10 and SK-MEL-2. concentration inhibiting cell growth up to below 40% of control was recognized at $10^{-3}g/ml$ of SBSK. In Inhibitory effect on activity of DNA topoisomerase I. the $IC_{50}$ was shown $200-400{\mu}g/ml$ of SBSK. The T/C was 154% in SBSK-treated group in S-180 bearing ICR mice, The concentration inhibiting adhesion of A549 and B16-F10 to complex extracellular matrix up to below 30% of control was recognized at $5{\times}10^{-4}$, $1{\times}10^{-3}\;g/ml$ of SBSK. In pumonary colonization assay with B16-BL/6, a number of colonies in the lungs were decreased significantly in SBSK-treated group as compared with control group, In hematological changes in B16-BL/6 injected C57BL/6, numbers of WBC and platelet were not changed significantly in SBSK-treated groups, In CAM and in vitro neovascularization assay, angiogenesis was inhibited significantly in SBSK-treated group as compared with control group. From above results it was concluded that SBSK could be usefully applied for the prevention and treatment of cancer.

• Asian Pacific Journal of Cancer Prevention
• /
• 제14권5호
• /
• pp.3023-3028
• /
• 2013

Objective: Photodynamic therapy (PDT ) is a promising modality for the treatment of various tumors. In order to assist in optimizing treatment, we applied 5-ALA/PDT in combination with low-dose cisplatin to evaluate cytotoxicity in Hela cells. Methods: Antiproliferative effects of 5-ALA/PDT and cisplatin, alone and in combination, were assessed using MTT assay. To examine levels of apoptosis, Hela cells treated with 5-ALA/PDT, and combination treatment were assessed with Annexin-V/PI by flow cytometry. To investigate the molecular mechanisms underlying alterations in cell proliferation and apoptosis, Western blot analysis was conducted to determine the expression of p53, p21, Bax and Bcl-2 proteins. Results: MTT assays indicated that combination treatment obviously decreased the viability of Hela cells compared to individual drug treatment. In addition, it was confirmed that exposure of Hela cells to 5-ALA/PDT in combination with low-dose cisplatin resulted in more apoptosis in vitro. Synergistic anticancer activity was related to upregulation p53 expression and alteration in expression of p21, Bcl-2 and Bax. Conclusion: Our findings suggest that administration of 5-ALA/PDT in combination with the low-dose cisplatin may be an effective and feasible therapy for cervical cancer.

• Journal of Ginseng Research
• /
• 제33권2호
• /
• pp.99-103
• /
• 2009

The in-vitro immunomodulatory function of murine natural-killer (NK) cells induced by red-ginseng acidic polysaccharide (RGAP) in ovariectomized (OVX) rats was examined in this study. The IL-2-induced NK cell activity was significantly decreased in the OVX rats compared to the sham groups, but the normally induced NK cell activity was not. RGAP, however, increased the NK cell activity in both groups, and this effect involved iNOS expression. The inhibition of iNOS activity did not increase the NK cell cytotoxicity by RGAP in the OVX rats. The data that were obtained also demonstrated that the expression of iNOS was increased in the spleen of the OVX rats. These results indicate that RGAP increases the tumoricidal activity of the NK cell in the OVX rats, which is a primed or activated state of innate immune cells resulting from the changes in cytokine production induced by estrogen-deficient stress. Therefore, RGAP has a synergistic effect on the NK cell activities, which are regulated by the iNOS signals in OVX rats. This suggests that RGAP is useful for potential therapeutic strategies as a nutrient in regulating the NK cells in OVX rats.

• 한방안이비인후피부과학회지
• /
• 제14권1호
• /
• pp.154-166
• /
• 2001

Naesosan(NSS) has been used in Oriental Medicine as a drug that treated carbuncle and cellulitis. So, the purpose of this Study was to investigate effects of NSS on the cytotoxicity of cancer cell lines and lymphocytes in vitro, proliferation of Ll210 cells and lymphocytes in L1210 cells transplanted mice, improvement of blood count in Ll210 cells transplanted mice, tumor weight and body weight in sarcoma-180 cells transplanted mice, survival prolongation in sarcoma-180 cells transplanted mice. We used NSS extract with freeze-dried, 8wks-old male mice(balb/c and ICR mouse $18{\pm}2g$). Ll210 cell lines, and sarcoma-180 cell lines for this Study, The proliferation of cells was tested using a colorimetric tetrazoliun assay(MTT assay). The results of this Study were obtained as follows ; 1. NSS showed significantly cytotoxicitic effects of cancer cell lines, did not show cytotoxicitic effects of lymphocytes. 2, Proliferation of lymphocytes in L1210 cells transplanted mice did not effects by NSS. 3. NSS inhibited significantly the proliferation of L1210 cells in L1210 cells transplanted mice. 4. NSS improved significantly the blood count in Ll210 cells transplanted mice. 5. NSS increased significantly th body weight in sarcoma-180 cells transplanted mice. 6. NSS dereased significantly the tumor weight in sarcoma-180 cells transplanted mice. 7. NSS prolonged significantly the survival time in sarcoma-180 cells transplanted mice.