• Korean Journal of Veterinary Research
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• v.42 no.3
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• pp.351-362
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• 2002

Newcastle disease (ND) is a highly contagious infection of poultry, Two pathology-based techniques, in situ RT-PCR and in situ hybridization (ISH) were applied to formalin-fixed, paraffin-embedded tissues from chickens naturally infected with velogenic ND virus (VNDV). Two pairs of primers and a probe for ISH and in situ RT-PCR, respectively, were selected from highly conserved region of matrix gene of NDV. The ISH experiment was carried out using MicroProbe$^{TM}$ capillary action system within 2 hours. In situ RT-PCR was performed using MicroProbe$^{TM}$ capillary action system and GeneAmp In Situ PCR system. With ISH and in situ RT-PCR, viral nucleic acid was detected in the central nervous system of chickens from infected with neurotropic velogenic Newcastle disease virus (NVNDV), whereas viral nucleic acid was detected in various organs or tissues of chickens from infected with viscerotropic velogenic Newcastle disease virus (VVNDV). In the NVND group, positive signals were characteristically defined in the cytoplasm of neuron, vascular endothelial cells, and perivascular mononuclear macrophages in the central nervous system. One of NVND group, chicken from one farm exhibited positive signals in the bronchial epithelium. The VVND group chickens showed positive reaction in the macrophages, vascular endothelium, and bronchiolar epithelium. Markedly, viral nucleic acid was detected in the macrophages of morphologically normal tissues which were peripheral or located in distant areas from lesions. The central nervous system of chickens infected with VVND virus had positive signals in the vascular endothelial cell, perivascular mononuclear macrophages and some neuron. The number and intensity of the positive cells by in situ RT-PCR were more and stronger, respectively, in comparison with those by ISH. Particularly, positive reaction was detected in macrophages infiltrating in cardiac muscle by in situ RT-PCR, but not obtained by ISH. Therefore, these results demonstrated that ISH is a rapid diagnostic method for detection of NDV and in situ RT-PCR can be used as an efficient method for detection of low viral load infection or subclinical viral infection of NDV.

• Biomedical Science Letters
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• v.2 no.2
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• pp.257-265
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• 1996

Simple stain and electron microscopic in situ hybridization is studied and applied for the identification of rabbit haemorrhagic disease viral RNA in a unicrylated preparation of the liver after innoculation of rabbit haemorrhagic disease virus. Hybridization for detection of viral RNA in unicryl embedded tissues using complementary 84 bases oligonucleotide probe labelled by biotin CE-phosphoramidite compared with 4717∼4800 sequences of rabbit haemorrhagic disease virus, modified hybridization protocol and antibiotin antibody-l0nm gold as signal marker. The best results were obtained in 0.02% glutaraldehyde, Unicryl resin cell block, biotinylated oligonucleotide probes, antibiotin-l0nm gold. In this report, RHD viral RNA was distributed widely within the mitochondria and nucleus of liver cell by electron microscopic in situ hybridization. In situ hybridization has become a standard method for localizing DNA or RNA sequences in tissue or celt preparation. In situ hybridization is detected the virus genome in the cells and tissue as specifically compared with others nucleic acid hybridization method.

• The Journal of the Acoustical Society of Korea
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• v.11 no.2
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• pp.15-27
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• 1992

The standard measuring method of the frequency characteristics of headphones has been needed because different results come from the different measuring methods because of the lack of the reasonable measuring method of headphone characteristics, for example, in the case of psycho-acoustic experiments with headphones. In this paper, based on this fact, we studied the measurement method of headphones based on the natural hearing condition of human being, that is in situ measuring method, by measuring the headphone frequency characteristics using an artificial ear and a newly proposed device, HATS(Head And Torso Simulator). From the result of this paper, we could see that the method appropriate to a so called in situ condition was the one wi9th HATS, Because HATS simulated more correctly the acoustic impedance of the ear and the diffraction effect of the human head than the artificial ear.

• The Journal of Korean Society of Virology
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• v.30 no.2
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• pp.125-130
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• 2000

Infection with human cytomegalovirus (HCMV) is of considerable clinical relevance after placental transmission and in immunosuppressed patients such as transplant recipients or patients with AIDS. The rapid detection method of HCMV has been required to overcome the time-consuming methods such as classical plaque assay or other immunological methods. This study was performed to establish the in situ ELISA, in which human lung fibroblasts infected with HCMV were fixed and used directly as antigen in 96 well culture plate. Expressed HCMV antigens were detected with HCMV-specific monoclonal antibodies. This method could detect HCMV dose-dependently upto $3{\times}10^2\;pfu/ml$. Antiviral activity of ganciclovir could be assayed within the known range of effective dose. This result showed that HCMV could be quantitated by in situ ELISA. The chemical, which was selected on the basis of component analysis in natural product, was tested to have the anti-HCMV activity by in situ ELISA, and three among five samples were found to have anti-HCMV activity with the dose-dependent manner. Conclusively in situ ELISA could be useful method for quantitation of HCMV and screening antiviral activity of samples to HCMV.

• Journal of Korean Tunnelling and Underground Space Association
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• v.9 no.3
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• pp.275-286
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• 2007

In construction of a structure in underground space, in-situ stress in rock mass has great effect on the stability of the structure. Especially, the direction and magnitude of rock stress have influence on the excavation method, the choice of support and reinforcement method for establishing the stability of tunnel. Therefore, it is very important to consider the characteristics of in-situ stress in rock mass for tunnel stability analysis. In this study, a reasonable design method for underground structure was reviewed through the case study for tunnel design considering in-situ rock stress. For this purpose, the estimation for SRF (Stress Reduction Factor) as input parameter in rock classification using Q-System and the assesment for tunnel support were studied. Also, considering the characteristics of in-situ rock stress such as the magnitude of K and the direction of principal stress, the parameter studies for tunnel stability analysis were carried out. An improved method was proposed for obtaining the better results in the tunnel stability analysis.

• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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• 2007.11a
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• pp.987-990
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• 2007

The accuracy of sound strength (G) measurement method in ISO 3382 has been questioned. One of the main reason is the difficulty in measuring a reference sound level in an anechoic chamber with the same set-up which was applied for the actual hall measurements. In the present paper, an in-situ measurement method for G was proposed by investigating the present G measurement method shown in ISO 3382. In addition, the sound radiation characteristics of typical omnidirectional loudspeakers were investigated and Phi (O) of auto correlation function (ACF) parameters was also calculated from an actual music excerpt to characterize the sound energy distribution in concert halls.

• Geomechanics and Engineering
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• v.8 no.6
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• pp.783-799
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• 2015

In this study, effect of horizontal in situ stress on failure mechanism around underground openings excavated in isotropic, elastic rock zones is investigated. For estimating the plastic zone occurrence, an induced stress influence area approach (Bray Equations) was modified to define critical stress ratio according to the Mohr-Coulomb failure criterion. Results obtained from modified calculations were compared with results of some other analytical solutions for plastic zone thickness estimation and the numerical modelling (finite difference method software, FLAC2D) study. Plastic zone and its geometry around tunnels were analyzed for different in situ stress conditions. The modified equations gave similar results with those obtained from the other approaches. However, safer results were calculated using the modified equations for high in situ stress conditions and excessive ratio of horizontal to vertical in situ stresses. As the outcome of this study, the modified equations are suggested to use for estimating the plastic zone occurrence and its thickness around the tunnels with circular cross-section.

• Journal of The Korean Society of Agricultural Engineers
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• v.50 no.5
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• pp.73-81
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• 2008

This study evaluated the applicability of in-situ concrete pile as a stabilization materials of embankment slopes including agricultural reservoir and rural road etc. The experimental embankment slopes was constructed to investigate the strength properties of in-situ concrete pile with embankment materials and boring methods. The test variable were applied the boring method(driving and augering) and water-cement ratio. In order to analyze the physical and mechanical properties of embankment materials, permeability and water contents test were was performed. Also, the freshly and harden of in-situ concrete properties were measured by the slump and compressive strength tests. The results showed the water content and permeability of embankment materials and boring methods affected on compressive strength of in-situ concrete pile.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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• 2006.11a
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• pp.278-279
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• 2006

In this study, we investigated the electro-optical (EO) characteristic of fringe-field switching (FFS) mode cell by the two kinds of ultraviolet (UV) alignment method on the organic thin film (polyimide Pl). The suitable organic layers for FFS cell and the aligning capabilities of nematic liquid crystal (NLC) using the in-situ photo-alignment method were studied. An unstable V-T curve of UV-aligned FFS-LCD with conventional photo-alignment method can be achieved. However, a stable V-T curve of UV-aligned FFS-LCD with in-situ photo-alignment method (1h), and V-T curve of UV-aligned FFS-LCD with in-situ photo-alignment method was much stable comparing with that of other UV-aligned FFS-LCD's, As a result, more stable EO performance of UV-aligned FFS-LCD with in-Situ photo-alignment method is obtained than that of the other UV-aligned FFS-LCD's.

• Journal of the Korean Institute of Electrical and Electronic Material Engineers
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• v.35 no.5
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• pp.431-438
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• 2022

In functional materials, in situ experimental techniques as a function of external stimulus (e.g., electric field, magnetic field, light, etc.) or changes in ambient environments (e.g., temperature, humidity, pressure, etc.) are highly essential for analyzing how the physical properties of target materials are activated/evolved by the given stimulation. In particular, in situ electric-field-dependent X-ray diffraction (XRD) measurements have been extensively utilized for understanding the underlying mechanisms of the emerging electromechanical responses to external electric field in various ferroelectric, piezoelectric, and electrostrictive materials. This tutorial article briefly introduces basic principles/key concepts of in situ electric-field-dependent XRD analysis using a lab-scale XRD machine. We anticipate that the in situ XRD method provides a practical tool to systematically identify/monitor a structural modification of various electromechanical materials driven by applying an external electric field.