• Proceedings of the Korean Society of Developmental Biology Conference
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• 2003.10a
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• pp.111-111
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• 2003

Telomeres are the end of chromosomes and consist of a tandem repeat sequence of (TTAGGG)n and associated proteins. Telomerase is a ribonucleoprotein which act as a template for the synthesis of telomeric DNA. Telomeres are essential for chromosome stability and are related with cell senescence, apoptosis and cancer. Even though telomeres and telomerase have been studied extensively, very little is known about telomere dynamics in embryonic cells. This study was carried out to analyze the telomeres distribution and telomerase activity of chicken cells during embryonic and developmental stages. The target cells for analysing were sperms, ovulated ova, early embryonic cells and the cells from brain, heart, liver, kidney and germinal tissue in fetus. Telomeres distribution on target cells was analyzed by Q-FISH (Quantitation-Fluorescence in situ Hybridization) techniques using a chicken telomere repeat probe. Telomerase activity was performed by TRAP assay (Telomeric repeat Amplification Protocol) with target DNA. In results, the telomeres of chicken were found at the ends of all chromosomes. In addition, chicken had interstitial telomeres on chromosomes 1, 2 and 3. Telomerase activity was highly detectable in early embryonic cells, germinal tissues and kidney cells. Whereas telomerase activity was gradually down-regulated when the organs, including brain, heart, and liver, were developed from embryos. In the distribution of telomeric DNA on the embryonic and developmental stages, most of the cells was gradually decreased in telomere quantity during ontogenesis.

• Proceedings of the KIEE Conference
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• 2001.07c
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• pp.1921-1923
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• 2001

In this work, an array of resistance temperature detector(RTD) was fabricated inside the microchannel in order to investigate in-situ flow characteristics. A rectangular straight microchannel, integrated with RTD's for temperature sensing and a heat source for generating the temperature gradient along the channel. were fabricated with the dimension of $200{\mu}m(W){\times}{\mu}m(D){\times}$48mm(L), while RTD measured precise temperatures at the inside-channel wall. 4" $525{\pm}25{\mu}m$ thick P-type <100> Si wafer was used as a substrate. For the fabrication of RTDs. 5300$\AA$ thick Pt/Ti layer was sputtered on a Pyrex glass wafer. Finally, glass wafer was bonded with Si wafer by anodic bonding, therefore RTD was located inside the microchannel. The temperature distribution inside the fabricated microchannel was obtained from 4 point probe measurements and Dl water is used as a working fluid. Temperature distribution inside the microchannel was measured as a function of mass flow rate and heat flux. As a result, precise temperatures inside the microchannel could be obtained. In conclusion, this novel temperature distribution measurement system will be very useful to the accurate analysis of the flow characteristics in the microchannel.

• Ocean and Polar Research
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• v.30 no.2
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• pp.181-191
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• 2008

This study aims at assessment of spatio-temporal distribution of demersal fish aggregations near the west coast of Jeju Island using hydroacoustic survey. A 200 kHz split beam transducer attached to a small towed body was used for all acoustic investigations. The received acoustic data were in situ acoustic target strength (TS, dB) for all pings and nautical area scattering coefficient(NASC, $m^2/mile^2$) for 0.1 mile along 12 acoustic transects. Demersal fish aggregations are distributed around the coastal slope having 20 to 30 m depth throughout all seasons. The concentration is higher during the summer season. With regard spatial distribution, higher demersal fish aggregations have been detected near the West coast of Shinchang and especially near Chagwi-do. Pelagic fish aggregations were higher to the south of Chagwi-do during the spring season. Additionally, standing stock of demersal fish aggregations from the NASC data, TS function, and length-weight function of dominant species was estimated as follows: 3.2 ton (CV 21.8%) in December 2006, 17.9 ton (CV 21.6%) in April 2007, 30.8 ton (CV 17.8%) in June 2007, and 22.5 ton (CV 24.2%) in October 2007. The application of hydroacoustic methods offers a new approach to understanding spatiotemporal structure and estimate the biomass of demersal fish aggregations in the coastal area. And the results can be made up limitations of qualitative analysis through net and diving for fisheries resources survey in coastal area.

• Korean Journal of Environment and Ecology
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• v.34 no.6
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• pp.503-514
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• 2020

Climate change leads to changes in phenological response and movement of plant habitats. Korea's evergreen broad-leaved forest has widened its distribution area compared for the past 20 years, and the range of its native habitats is moving northward. We analyzed climate indices such as the warmth index, the cold index, the lowest temperature in the coldest month, and the annual average temperature, which are closely related to vegetation distribution, to predict the change in the native habitat of Lauraceae evergreen broad-leaved trees. We also analyzed the change and spatial distribution to identify the habitat climate characteristics of 8 species of Lauraceae evergreen broad-leaved trees distributed in the warm temperate zone in Korea. Moreover, we predicted the natural habitat change in the 21st century according to the climate change scenario (RCP 4.5/8.5), applying the MaxEnt species distribution model. The monthly average climate index of the 8 species of Lauraceae evergreen broad-leaved trees was 116.9±10.8℃ for the temperate index, the cold index 3.9±3.8℃, 1495.7±455.4mm for the annual precipitation, 11.7±3.5 for the humidity index, 14.4±1.1℃ for the annual average temperature, and 1.0±2.1℃ for the lowest temperature of winter. Based on the climate change scenario RCP 4.5, the distribution of the Lauraceae evergreen broad-leaved trees was analyzed to expand to islands of Jeollanam-do and Gyeongsangnam-do, adjacent areas of the west and south coasts, and Goseong, Gangwon-do on the east coast. In the case of the distribution based on the climate change scenario RCP 8.5, it was analyzed that the distribution would expand to all of Jeollanam-do and Gyeongsangnam-do, and most regions except for some parts of Jeollabuk-do, Chungcheongnam-do, Gyeongsangbuk-do, and the capital region. For the conservation of Lauraceae evergreen broad-leaved trees to prepare for climate change, it is necessary to establish standards for conservation plans such as in-situ and ex-situ conservation and analyze various physical and chemical characteristics of native habitats. Moreover, it is necessary to preemptively detect changes such as distribution, migration, and decline of Lauraceae evergreen broad-leaved trees following climate change based on phenological response data based on climate indicators and establish conservation management plans.

• Ocean and Polar Research
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• v.22 no.2
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• pp.81-90
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• 2000

Supposed construction of a large port in the mouth of Tuman River requires careful examination of possible unfavorable ecological consequences for the Far Eastern Federal Marine Reserve. Since the Tuman River is the largest source of suspended material and possible contaminants flowing into the sea, and in order to understand how this material is allocated in the coastal zone, analyses are needed to check possible pathways of water transport and circulation system in the region. Linearized shallow water equations were used for numerical simulation of the wind-driven circulation to the north off the Tuman River mouth. The model results satisfactorily agreed with in situ data. The model circulation patterns are largely dependent on the wind direction and are conformed by the distribution of bottom sediments, and by the location of organic carbon and some pollutants accumulation zones. The most unfavorable situation for the Marine Reserve is the case of the southwesterly wind; even with quite moderate wind, the waters polluted by the run-off from the Tuman River can attain the south section of the Marine Reserve during the diurnal period.

• Environmental Engineering Research
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• v.11 no.5
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• pp.241-249
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• 2006

A mathematical model is described for the prediction of convective upward transport of an organic solvent driven by evaporation at the surface, which is known as the major transport mechanism in the in-situ photolysis of a soil contaminated with 2,3,7,8-tetrachlorodibenzo-p-dioxin(TCDD). A finite-element model was proposed to incorporate the effects of multiphase flow on the distribution of each fluid, gravity as a driving force, and the use of hysteretic models for more accurate description of k-S-p relations. Extensive numerical calculations were performed to study fluid flow through three types of soils under different water table conditions. Predictions of relative permeability-saturation-pressure (k-S-p) relations and fluids distribution for an illustrative soil indicate that hysteresis effects may be quite substantial. This result emphasizes the need to use hysteretic models in performing flow simulations including reversals of flow paths. Results of additional calculations accounting for hysteresis on the one-dimensional unsaturated soil columns show that gravity affects significantly on the flow of each fluid during gravity drainage, solvent injection, and evaporation, especially for highly permeable soils. The rate and duration of solvent injection also have a profound influence on the fluid saturation profile and the amount of evaporated solvent. Key factors influencing water drainage and solvent evaporation in soils also include hydraulic conductivity and water table configuration.

• The Journal of Industrial Distribution & Business
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• v.9 no.10
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• pp.21-37
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• 2018

Purpose - This paper explores the contribution lean makes to service levels by addressing lean thinking application in logistics process improvement within a MNC soft drinks manufacturer situated in the United Arab Emirates, and identifies the most significant sources of wastes and their interdependence and highlights key attributes of successful lean implementation. Research design, data, and methodology - This case study examines key logistics employees' perceptions on lean methodology. Results - The results highlight the most significant types of wastes in logistical processes and illustrates ways to eliminate wastes and streamline process flow. The importance of the human role within lean methodology is also identified. Findings suggest that managers should follow both a micro and macro approach when implementing lean practices, as focusing on one waste often leads to ignoring the significance of other wastes. Conclusions - This study highlights the importance of implementing lean at both micro and macro levels and shows how staff involvement increases the efficacy of lean thinking. It offers guidance towards the efficiency of creating value stream mapping processes in situ, which considers different wastes and their interdependence. Managers with limited resources are encouraged to implement lean methodology taking cognizance of these factors.

• Reproductive and Developmental Biology
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• v.30 no.4
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• pp.263-270
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• 2006

The Chinese Hamster Ovarian cells CHO-K1 are one of the most extensively used cells for the evaluation of gene expression and toxicology. However, these cells are frequently used for biomedical research without consideration of their cytogenetic characteristics. Therefore, we carried out to investigate the karyologic profiles, the frequency and type of chromosome aberration, and the distribution of telomeric DNA on chromosomes of the CHO-K1 cells. The GTG banding and fluorescence in situ hybridization on CHO-K1 cells were performed to characterize the karyotype and the distribution of telomeric DNA The present study revealed that the chromosome modal number of CHO-K1 cells was 2n=20; eight chromosomes appeared to be identical with those of the normal Chinese hamster, whereas the remaining 12 chromosomes were shown to be translocated, deleted, inversed, or rearranged from Chinese hamster chromosomes. The telomeric DNA on CHO-K1 chromosomes was intensively distributed at the centromeres rather than the ends of chromosomes. In addition, three chromosomes had interstitial telomeres and one marker chromosome entirely consisted of telomeric DNAs. The frequency and type of chromosome aberrations in CHO-K1 cells were examined. Of the 822 metaphase spreads, 68 (8.3%) cells resulted in chromosome aberrations of which the chromosome breakage was the most frequently occurred.

• Macromolecular Research
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• v.16 no.4
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• pp.329-336
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• 2008

Core/shell ($\pm$)PS/(-)silica nanocomposite particles were synthesized by dispersion polymerization using an amphoteric initiator, 2,2'-azobis [N-(2-carboxyethyl)-2,2-methylpropionamidine] ($HOOC(CH_2)_2HN$(HN=) $C(CH_3)_2CN$=NC $(CH_3)_2C$(=NH)NH $(CH_2)_2COOH$), VA-057. Negatively charged (-6.9 mV) silica was used as the stabilizer. The effects of silica addition time and silica and initiator concentrations were investigated in terms of polymerization kinetics, ultimate particle morphology, and size/size distribution. Uniform hybrid microspheres with a well-defined, core-shell structure were obtained at the following conditions: silica content = 10-15 wt% to styrene, VA-057 content=above 2 wt% to styrene and silica addition time=0 min after initiation. The delay in silica addition time retarded the polymerization kinetics and broadened the particle size distribution. The rate of polymerization was strongly affected by the silica content: it increased up to 15 wt% silica but then decreased with further increase in silica content. However, the particle size was only marginally influenced by the silica content. The zeta potential of the composite particles slightly decreased with increasing silica content. With increasing VA-057 concentration, the PS microspheres were entirely coated with silica sol above 1.0 wt% initiator.

• Journal of Environmental Science International
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• v.23 no.8
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• pp.1481-1493
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• 2014

In order to understand the relation between the distribution of sea surface temperature and heavy snowfall over western coast of the Korean peninsula, several numerical assessments were carried out. Numerical model used in this study is WRF, and sea surface temperature data were FNL(National Center for Environment Prediction-Final operational global analysis), RTG(Real Time Global analysis), and OSTIA(Operational Sea Surface Temperature and Sea Ice Analysis). There were produced on the basis of remote sensing data, such as a variety of satellite and in situ observation. The analysis focused on the heavy snowfall over Honam districts for 2 days from 29 December 2010. In comparison with RTG and OSTIA SST data, sensible and latent heat fluexes estimated by numerical simulation with FNL data were higher than those with RTG and OSTIA SST data, due to higher sea surface temperature of FNL. General distribution of RTG and OSTIA SST showed similar, however, fine spatial differences appear in near western coast of the peninsula. Estimated snow fall amount with OSTIA SST was occurred far from the western coast because of higher SST over sea far from coast than that near coast. On the other hand, snowfall amount near coast is larger than that over distance sea in simulation with RTG SST. The difference of snowfall amount between numerical assessment with RTG and OSTIA is induced from the fine difference of SST spatial distributions over the Yellow sea. So, the prediction accuracy of snowfall amount is strongly associated with the SST distribution not only over near coast but also over far from the western coast of the Korean peninsula.