• Proceedings of the Korean Vacuum Society Conference
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• 2016.02a
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• pp.402.1-402.1
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• 2016

The performance of solid oxide fuel cells (SOFCs) is directly related to the electrocatalytic activity of composite electrodes in which triple phase boundaries (TPBs) of metallic catalyst, oxygen ion conducting support, and gas should be three-dimensionally maximized. The distribution morphology of catalytic nanoparticle dispersed on external surfaces is of key importance for maximized TPBs. Herein in situ grown nickel nanoparticle onto the surface of fluorite oxide is demonstrated employing gadolium-nickel co-doped ceria ($Gd0.2-xNixCe0.8O2-{\delta}$, GNDC) by reductive annealing. GNDC powders were synthesized via a Pechini-type sol-gel process while maximum doping ratio of Ni into the cerium oxide was defined by X-ray diffraction. Subsequently, NiO-GNDC composite were screen printed on the both sides of yttrium-stabilized zirconia (YSZ) pellet to fabricate the symmetrical half cells. Electrochemical impedance spectroscopy (EIS) showed that the polarization resistance was decreased when it was compared to conventional Ni-GDC anode and this effect became greater at lower temperature. Ex situ microstructural analysis using scanning electron microscopy after the reductive annealing exhibited the exsolution of Ni nanoparticles on the fluorite phases. The influence of Ni contents in GNDC on polarization characteristics of anodes were examined by EIS under H2/H2O atmosphere. Finally, the addition of optimized GNDC into the anode functional layer (AFL) dramatically enhanced cell performance of anode-supported coin cells.

• Applied Microscopy
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• v.51
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• pp.4.1-4.12
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• 2021

Fluorescence in situ hybridization (FISH) is a technique to visualize specific DNA/RNA sequences within the cell nuclei and provide the presence, location and structural integrity of genes on chromosomes. A confocal Whole Slide Imaging (WSI) scanner technology has superior depth resolution compared to wide-field fluorescence imaging. Confocal WSI has the ability to perform serial optical sections with specimen imaging, which is critical for 3D tissue reconstruction for volumetric spatial analysis. The standard clinical manual scoring for FISH is labor-intensive, time-consuming and subjective. Application of multi-gene FISH analysis alongside 3D imaging, significantly increase the level of complexity required for an accurate 3D analysis. Therefore, the purpose of this study is to establish automated 3D FISH scoring for z-stack images from confocal WSI scanner. The algorithm and the application we developed, SHIMARIS PAFQ, successfully employs 3D calculations for clear individual cell nuclei segmentation, gene signals detection and distribution of break-apart probes signal patterns, including standard break-apart, and variant patterns due to truncation, and deletion, etc. The analysis was accurate and precise when compared with ground truth clinical manual counting and scoring reported in ten lymphoma and solid tumors cases. The algorithm and the application we developed, SHIMARIS PAFQ, is objective and more efficient than the conventional procedure. It enables the automated counting of more nuclei, precisely detecting additional abnormal signal variations in nuclei patterns and analyzes gigabyte multi-layer stacking imaging data of tissue samples from patients. Currently, we are developing a deep learning algorithm for automated tumor area detection to be integrated with SHIMARIS PAFQ.

• Journal of Environmental Science International
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• v.21 no.12
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• pp.1455-1462
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• 2012

The vertical distributions of nitrifying bacteria in aerobic fixed biofilm were investigated to evaluate the relationship between nitrification performance and microbial community at different HRT. Fluorescent in situ hybridization (FISH) and portable ion selective microelectrode system were adopted to analyze microbial communities and ions profiles according to the biofilm depth. Cilia media packed MLE (Modified Ludzack-Ettinger) like reactor composed of anoxic, aerobic I/II was operated with synthetic wastewater having COD 200 mg/L and $NH_4{^+}$-N mg/L at HRT of 6 hrs and 4 hrs. Total biofilm thickness of aerobic I, II reactor at 4 hrs condition was over two times than that of 6 hrs condition due to the sufficient substrate supply at 4 hrs condition (6 hrs; aerobic I 380 ${\mu}m$ and II 400 ${\mu}m$, 4 hrs; aerobic I 830 ${\mu}m$ and II 1040 ${\mu}m$). As deepen the biofilm detection point, the ratio of ammonia oxidizing bacteria (AOB) was decreased while the ratio of nitrite oxidizing bacteria (NOB) was maintained similar distribution at both HRT condition. The ratio of AOB was higher at 4 hrs than 6 hrs condition and $NH_4{^+}$-N removal efficiency was also higher at 4 hrs with 89.2% than 65.4% of 6 hrs. However, the ratio of NOB was decreased when HRT was reduced from 6 hrs to 4 hrs and $NO_2{^-}$-N accumulation was observed at 4 hrs condition. Therefore, it is considered that insufficient HRT condition could supply sufficient substrate and enrichment of AOB in all depth of fixed biofilm but cause decrease of NOB and nitrite accumulation.

• The Bulletin of The Korean Astronomical Society
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• v.37 no.2
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• pp.118.1-118.1
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• 2012

In situ observations from the Wind spacecraft that statistically analyzed the solar wind proton at 1 AU has indicated that the measured proton temperature anisotropies seems to be regulated by the oblique instabilities (the mirror and oblique firehose). This result is in contradiction with the prediction of linear kinetic theory that the ion-cyclotron (for ${\beta}_{\parallel}$ < 2) and parallel firehose (for ${\beta}_{\parallel}$ <10) would dominate over the oblique instabilities. Various kinds of physical mechanisms have been suggested to explain this disagreement between the observations and linear theory. All of the suggestions consider the solar wind as a unoform magnetized plasma. However the real space environment is replete with the intermediate spatio-temporal scale variations associated with various physical quantities, such as the magnetic field intensity and the solar wind density. In this paper we present that the pervasive intermediate-scale temporal variation of the local magnetic field intensity can lead to the modification of the proton temperature anisotropy versus beta inverse correlation for temperature-anisotropy-driven instabilities. By means of quasilinear kinetic theory involving such temporal variation, we construct the simulated solar wind proton data distribution associated the magnetic fluctuations in (${\beta}_{\parallel}$, $T_{\perp}/T_{\parallel}$) space. It is shown that the theoretically simulated proton distribution and a general trend of the enhanced fluctuations bounded by the oblique instabilities are consistent with in situ observations. Furthermore, the measure magnetic compressibility can be accounted for by the magnetic spectral signatures of the unstable modes.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.51 no.2
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• pp.272-278
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• 2015

The aim of this study is to find out the behavior and acoustic backscattering of the large jellyfish Nemopliema nomurai using hydroacoustics in situ. N. nomurai was distributed at depths ranging from 10~15 m during the day. Regarding the behavior of N. nomurai, there was no significant change in depth, and 3D tortuosity was not high. The vertical direction was ${\pm}10^{\circ}$ from the horizontal, and moving speed was $0.9{\sim}1.5\;m\;s^{-1}$. With regard to hydro-acoustical characteristics, the mean TS of N. nomurai ranged from -69.6~-56.0 dB at 38 kHz and -69.4~-54.5 dB at 120 kHz. TS variation (Max TS-Min TS) at 38 and 120 kHz was 0~10.2 dB and 0.2~16.0 dB, respectively. Mean TS and TS variation (Max TS-Min TS) of N. nomurai were higher at 120 kHz than at 38 kHz. The results showed that the use of hydroacoustics was effective in estimating the distribution depth, behavior, and acoustic characteristics of the target.

• Korean Journal of Mineralogy and Petrology
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• v.34 no.1
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• pp.83-93
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• 2021

NASA has a plan for the Artemis manned lunar mission in 2020. In 2030s, not only America but also other countries are considering to prepare for human to stay on the Moon at least for a month and necessary technology is currently being developed. With this plan, the mostly considered thing is lunar in-situ resource utilization. The most essential resources could be water and oxygen for sustain human life on the Moon. These resources are not supposed to be brought from the Earth, and it is economically sensible if they are obtained from the lunar surface. Because oxygen can be used as both oxidizer and propellent when a rocket departs from a lunar base directly to Mars, technology for extraction method of oxygen resource and its utilization has been being developed worldwide. This paper introduces oxygen distribution on the Moon and major oxygen extraction methods.

• Applied Microscopy
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• v.18 no.2
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• pp.167-176
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• 1988

Extrachromosomal circular DNA complexes from erythrocytes and splenocytes isolated from Carassius carassius were examined by mica-press-absorption method. The method was described that released small polydisperse circular DNA molecules in situ from the erythrocytes and the splenocytes and that allows selective observation of the small circular DNA complexes bound to cellular components. The released polydisperse circular DNA complexes were absorbed preferentially on mica in a divalent cation-free medium then processed for electron microscopy. Small circular DNAs showed a heterogeneous size distribution of $2{\sim}10{\mu}m$ with a mean contour length of $4.3{\mu}m$ for the circulating erythrocytes and that of $0.7{\sim}3.6{\mu}m$ with a mean contour of length $2.04{\mu}m$ for the splencytes. Cells contained $100{\sim}300$ copies and $300{\sim}700$ copies obtained from the erythrocytes and the splenocytes, repectively. Possible biological functional implications for size distribution of extrachromosomal circular DNAs are discussed.

• Textile Coloration and Finishing
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• v.10 no.2
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• pp.37-44
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• 1998

In this study, the polyurethane microcapsules containing disperse dye were prepared by in-situ polymerization method using hexamethylene diisocyanate (HDI, aliphatic type) and m-xylene diisocyanate(XDI, aromatic type) with ethylene glycol (EG) And the size, shape, particle size distribution, and breaking behavior of microcapsules prepared were investigated. The size and shape of microcapsule were observed by scanning electron microscope (SEM) . The particle size distribution was analyzed by image analyzer. The breaking behavior of microcapsule was checked by measuring the optical density of solution that the disperse dye was dissolved after the microcapsule was broken by constant pressure. The particle size was inversely proportional to the stirring speed, and the size of microcapsule prepared from HDI and EG was smaller than that of microcapsule prepared from XDI and EG. Aliphatic type microcapsule was broken easily, compared with aromatic type one. It was considered due to the difference of reactivity between HDI and XDI. And the microcapsule prepared by stirring strongly was broken easily.

• Journal of the Korean Geosynthetics Society
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• v.16 no.1
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• pp.73-83
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• 2017

Laboratory and in-situ tests were conducted to evaluate the cone factors for the layers with low plasticity containing a lot of silty and sand soils from the west coast (Incheon, Hwaseong and Gunsan areas) and its applicability was evaluated based on these results. The cone factors were evaluated from 19 to 23 based on unconfined compression strengths (qu), from 13 to 13.8 based on simple CU strengths and from 11.6 to 13.1 based on field vane strengths, respectively. The unconfined compression strengths of undisturbed silty soil samples with low plasticity were considerably underestimated due to the change of in-situ residual effective stress during sampling. Half of unconfined compression strength (qu/2) based cone factors of silty soils with low plasticity fluctuated and were approximately 1.8 times higher than simple CU based values of these soils. When evaluating cone factors of these soils, it should be judged overall on the physical properties such as the grain size distribution and soil plasticity and on the fluctuation of the corrected cone resistance and the sleeve friction due to the distribution of sandseam in the ground including pore pressure parameter.

• Ocean and Polar Research
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• v.28 no.3
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• pp.225-236
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• 2006

Seagrass meadows are considered as critical habitats for a wide variety of marine organisms in coastal and estuarine ecosystems. In many cases, studies on the spatial/temporal distribution of seagrass have depended on direct observations using SCUBA diving. As an alternative method fur studying seagrass distribution, an application of hydroacoustic technique has been assessed for mapping seagrass distribution in Dongdae Bay, on the south coast of Korea, in September 2005. Data were collected using high frequency transducer (420 kHz split-beam), which was installed with towed body system. The system was linked to DGPS to make goo-referenced data. Additionally, in situ seagrass distribution has been observed using underwater cameras and SCUBA diving at four stations in order to compare with acoustic data. Acoustic survey was conducted along 23 transects with 3-4 blot ship speed. Seagrass beds were vertically limited to depths less than 3.5m and seagrass height ranged between 55 and 90cm at the study sites. Dense seagmss beds were mainly found at the entrance of the bay and at a flat area around the center of the bay. Although the study area was a relatively small, the vertical and spatial distributions of the seagrass were highly variable with bathymetry and region. Considering dominant species, Zostera marina L., preliminary estimation of seagrass biomass with acoustic and direct sampling data was approximately $56.55g/m^2$, and total biomass of 104 tones (coefficient variation: 25.77%) was estimated at the study area. Hydroacoustic method provided valuable information to understand distribution pattern and to estimate seagrass biomass.