• Title/Summary/Keyword: In Situ Degradation

검색결과 231건 처리시간 0.025초

Ruminal Degradability of Tropical Feeds and Their Potential Use in Ruminant Diets

  • Chanjula, P.;Wanapat, M.;Wachirapakorn, C.;Uriyapongson, S.;Rowlinson, P.
    • Asian-Australasian Journal of Animal Sciences
    • /
    • 제16권2호
    • /
    • pp.211-216
    • /
    • 2003
  • The objective of this study was to determine the degradability of cassava chip (CC), cassava waste (CW), yellow sweet potato (YP), white sweet potato (WP), purple sweet potato (PP), corn meal (CM), and rice bran (RB) using in situ technique. Two ruminally fistulated steers with an average weight of $303{\pm}10kg$ were used to determine in situ degradabilities of DM and OM. Seven feed sources were weighted in nylon bags ($38{\mu}m$ pore size) and incubated ruminally for 1, 2, 4, 6, 8, 12, 24, and 48 h. The results showed that asymptote (a+b) and effective degradability (ED) of DM of energy sources ranked from the highest to the lowest; CC, YP, WP, PP, RB, CW, and CM (99.3, 92.5; 97.6, 87.9; 97.5, 87.9; 97.2, 87.8; 87.5, 63.6; 78.6, 63.0 and 81.7; 59.3, respectively) and for OM asymptote (a+b) and effective degradability (ED) were similar to those of degradation of DM (99.4, 93.4; 98.8, 89.8; 98.5, 89.4; 98.4, 88.1; 92.4, 65.8; 85.1, 66.9 and 83.6, 63.3, respectively). It was concluded that disappearance characteristic of CC was the highest and it may potentially facilitate the achievement of optimal ruminal availability of energy: protein especially with NPN for microbial protein synthesis.

액정 폴리에스테르와 폴리(에틸렌 테레프탈레이트)의 복합재료 연구 (On the Composites of Poly(ethylene terephthalate) with a Liquid Crystalline Polyester)

  • 최재곤;방문수;한철
    • 공업화학
    • /
    • 제8권1호
    • /
    • pp.76-83
    • /
    • 1997
  • 열방성 액정고분자(TLCP)와 폴리(에틸렌 테레프탈레이트)를 혼합용매 중에서 블렌딩하였으며, 이들 블렌드는 capillary rheometer die를 통하여 $287^{\circ}C$에서 섬유상 압출물로 가공되었다. 블렌드와 제조된 복합재료의 열적 성질, 기계적 성질, 모폴로지는 DSC, 편광현미경, SEM 및 인장시험에 의하여 조사되었다. 블렌드의 결정화 동력학은 등온 DSC 방법에 의하여 측정된 데이타로부터 Avrami식을 이용하여 결정화 속도 및 결정성장 메카니즘에 대한 정보를 얻었다. 블렌드내의 액정상은 가공온도조건하에서 거대상분킥나 열분해현상을 보이지 않았으며, 액체질소 속에서 절단된 섬유 단면의 SEM 관찰에 의하면, 섬유내 TLCP domain은 $0.1{\mu}m$에서 $0.2{\mu}m$정도의 크기로 분산되었고, 두 상 계면에서의 접착은 잘 되어있음을 알 수 있었다. TLCP/PET in-situ 섬유상 복합재료의 인장강도와 모듈러스는 TLCP 함량이 많을수록, draw ratio가 높을수록 증가됨을 알 수 있었다.

  • PDF

저온.담수토양에서 벼종자 $\beta$-아밀라제 유전자 발현과 호분층 인접 배유의 전분분해 양상 (Expression of $\beta$-amylase Gene and Degradation of Starch Granules of Germinating Rice Seed under Low Temperature and Submerged Soil Condition)

  • 윤병성;강원희
    • 한국작물학회지
    • /
    • 제47권6호
    • /
    • pp.413-417
    • /
    • 2002
  • 온대 수도작에 있어서 발아시의 조건에 가까운 저온.담수 토양조건에서의 출아에 관련된다고 생각되어지는 $\beta$-아밀라제 유전자 발현과 발현 양상이 실제로 호분층 인접 부분의 전분 분해에 영향을 미치는지 in situ hybridization과 현미화학적방법으로 검토하였다. 1. $18^{\circ}C$의 저온.담수토양조건하에서 출아했던 장향도 품종은 호분층에서 $\beta$-아밀라제 유전자의 발현이 보였다. 2. $18^{\circ}C$의 저온.담수토양조건하에서 출아하지 못했던 수원 287호는 $\beta$-아밀라제 유전자의 발현이 보이지 않았다. 3. $\beta$-아밀라제 활성의 유무에 의해 배반세포에 인접한 배유부분의 전분분해량에 변화를 보여 $\beta$-아밀라제 활성이 높은 장향도, 은방주(Ginbozu), Fortana I-133가 $18^{\circ}C$의 저온.담수토양조건하에서는 $\beta$-아밀라제 활성을 나타내지 않는 수원 287호와 시험한 모든 조건하에서 $\beta$-아밀라제의 활성이 보이지 않았던 농림(Norin) 6호, 고시히까리(Koshihikari) 보다 배반상피세포 및 배반상피세포에 인접한 호분층 근접 배유부분의 전분립 감소가 컸다. 이상의 결과 저온.담수토양조건하에서 벼 종자의 출아에 $\beta$-아밀라제 유전자의 발현과 전분 분해의 연관 가능성을 확인하였다.

디젤오염토양에 대한 지중 오존산화처리 적용 가능성 (The In-Situ Ozone Oxidative Remediation Potential of Diesel Fuel-contaminated Soil)

  • 유도윤;신응배;배우근
    • 한국토양환경학회지
    • /
    • 제4권3호
    • /
    • pp.3-15
    • /
    • 1999
  • 기존의 SVE 공정에 공기 대신 오존을 주입하여 유류물질의 지중분해를 유도하고 추출가스의 처리부담을 최소화할 수 있는 복원 신공정 개발을 위한 기초연구를 수행하였다. 이를 위하여 비휘발성 유류물질인 디젤유를 처리대상물질로 하여 토양칼럼실험을 통해 오존주입에 의한 지중분해 가능성을 검토하였다. 수분함량 8.39%의 실토양에 디젤유를 혼합하여(초기농도 1,485mg-DRO/kg-soil)칼럼에 충진시킨 후 119.0$\pm$6.1mg/L의 농도를 가지는 오존가스를 50mL/min의 유량으로 연속 주입하였을 때 14시간 반응후 칼럼 상 하부의 디젤유 제거율은 각각 87.9%, 100.0%를 나타내어 비교적 빠른 시간내에 효과적으로 처리되는 것으로 나타났다. 한편 동일한 조건에서 오존가스 대신 공기를 주입하였을 때 공기 유입 및 유출부 모두 총 14시간 동안의 접촉시간 동안 30%이내의 제거율을 나타냄에 따라 디젤오염토양에 대한 기존 SVE공정의 적용한계를 확인하였다.

  • PDF

High energy swift heavy ion irradiation and annealing effects on DC electrical characteristics of 200 GHz SiGe HBTs

  • Hegde, Vinayakprasanna N.;Praveen, K.C.;Pradeep, T.M.;Pushpa, N.;Cressler, John D.;Tripathi, Ambuj;Asokan, K.;Prakash, A.P. Gnana
    • Nuclear Engineering and Technology
    • /
    • 제51권5호
    • /
    • pp.1428-1435
    • /
    • 2019
  • The total ionizing dose (TID) and non ionizing energy loss (NIEL) effects of 100 MeV phosphorous ($P^{7+}$) and 80 MeV nitrogen ($N^{6+}$) ions on 200 GHz silicon-germanium heterojunction bipolar transistors (SiGe HBTs) were examined in the total dose range from 1 to 100 Mrad(Si). The in-situ I-V characteristics like Gummel characteristics, excess base current (${\Delta}I_B$), net oxide trapped charge ($N_{OX}$), current gain ($h_{FE}$), avalanche multiplication (M-1), neutral base recombination (NBR) and output characteristics ($I_C-V_{CE}$) were analysed before and after irradiation. The significant degradation in device parameters was observed after $100MeV\;P^{7+}$ and $80MeV\;N^{6+}$ ion irradiation. The $100MeV\;P^{7+}$ ions create more damage in the SiGe HBT structure and in turn degrade the electrical characteristics of SiGe HBTs more when compared to $80MeV\;N^{6+}$. The SiGe HBTs irradiated up to 100 Mrad of total dose were annealed from $50^{\circ}C$ to $400^{\circ}C$ in different steps for 30 min duration in order to study the recovery of electrical characteristics. The recovery factors (RFs) are employed to analyse the contribution of room temperature and isochronal annealing in total recovery.

의왕시내 BTEX 오염 부지에서의 자연 정화법 이용 적합성 고찰 (Assessment of Monitored Natural Attenuation as Remediation Approach for a BTEX Contaminated Site in Uiwang City)

  • 이민효;윤정기;박종환;이문순;강진규;이석영
    • 한국지하수토양환경학회:학술대회논문집
    • /
    • 한국지하수토양환경학회 1999년도 정기총회 및 춘계 공동 학술발표회
    • /
    • pp.149-156
    • /
    • 1999
  • In the United States (U.S.), the monitored natural attenuation (MNA) approach has been used as an alternative remedial option for organic and inorganic compounds retained in soil and dissolved in groundwater. The U.S. Environmental Protection Agency (EPA) defines the MNA as“in-situ naturally-occurring processes include biodegradation, diffusion, dilution, sorption, volatilization, and/or chemical and biochemical stabilization of contaminants and reduce contaminant toxicity, mobility or volume to the levels that are protective of human health and the environment”. The Department of Soil Environment. National Institute Environmental Research (NIER) is in the process for demonstrating the MNA approach as a potential remedial option for the BTEX contaminated site in Uiwang City. The project is charactering the research site in terms of the nature and extend of contamination, biological degradation rate, and geochemical and hydrological properties. The microbial-degradation rate and effectiveness of nutrient and redox supplements will be determined through laboratory batch and column tests. The geochemical process will be monitored for determining the concentration changes of chemical species involved in the electron transfer processes that include methanogenesis, sulfate and iron reduction, denitrification, and aerobic respiration. Through field works, critical soil and hydrogeologic parameters will be acquired to simulate the effects of dispersion, advection, sorption, and biodegradation on the fate and transport of the dissolved-phase BTEX plume using Bioplume III model. The objectives of this multi-years research project are (1) to evaluate the MNA approach using the BTEX contaminated site in Uiwang City, (2) to establish a standard protocol for future application of the approach, (3) to investigate applicability of the passive approach as a secondary treatment remedy after active treatments. In this presentation, the overall picture and philosophy behind the MNA approach will be reviewed. Detailed discussions of the site characterization/monitoring plans and risk-based decision-making processes for the demonstration site will be included.

  • PDF

IN SITU RUMINAL DEGRADATION KINETICS OF FORAGES AND FEED BYPRODUCTS IN MALE NILI-RAVI BUFFALO CALVES

  • Sarwar, M.;Mahmood, S.;Abbas, W.;Ali, C.S.
    • Asian-Australasian Journal of Animal Sciences
    • /
    • 제9권5호
    • /
    • pp.533-538
    • /
    • 1996
  • The rate and extent of digestion of dietary carbohydrates has a tremendous impact on ruminal fermentation and the productivity of the animals. The objective of the study was to determine the dry matter (DM) and neutral detergent fiber (NDF) degradabilities and rate and extent of feed byproducts (cotton seed cake, wheat bran), legumes [berseem (Egyptian clover), lucern (Medicago sativa), cowpeas (Vigna sinensis)], grasses [maize (Zea mays), millet (Panicum miliaceum), sorghum (Sorghum vulgare)] and wheat straw in ruminally fistulated male buffalo calves. By using nylon bags, 10 grams sample was exposed to the ruminal fermentation for 0, 1, 2, 4, 6, 10, 16, 24, 36, 48 and 96 hours. Dry matter and NDF degradability was measured at 48 hours. Extent of DM and NDF disappearance was determined at each time point. Rates of disappearance of DM and NDF were determined by regressing the natural logarithm of the percentage of original DM and NDF remaining in the bags between 1 and 96 hours. The dry matter digestibility (DMD) of the feed byproducts (FBP) and legume forages when incubated in the rumen of male buffalo calves were greater (p < 0.05) than grasses. Extent of digestion followed similar pattern as DMD. Rate of DMD was higher in FBP than in legumes and was the lowest in the wheat straw. The NDF degradability (NDFD) of FBP, legumes and grasses did not differ, however, wheat straw had the lowest NDFD from all the feeds tested. The lowest NDFD of wheat straw may have been due to the depressing effect of lignin on fiber digestion. The FBP and legumes had higher (p < 0.05) rates and lower extents of NDF digestion than grasses.

Effect of pH on the binding of hGM-CSF to ion exchange resin

  • Myoung, Hyun-Jong;Lee, Sang-Yoon;Lee, Kyoung-Hoon;Han, Kyu-Boem;Kim, Dong-Il
    • 한국생물공학회:학술대회논문집
    • /
    • 한국생물공학회 2003년도 생물공학의 동향(XIII)
    • /
    • pp.320-323
    • /
    • 2003
  • 본 연구에서는 형질전환된 식물세포배양으로 생산한 (hGM-CSF)를 cationic exchange resin인 CM-sepharose와 anionic exchange resin인 DEAE-sepharose에 통과키며 pH의 변화를 주었을 때 흡착 여부를 확인하였다. hGM-CSF는 pH가 5-7사이의 범위에서 가장 안정함을 보였으며 이 결과를 바탕으로 각각의 pH 범위를 결정하였다. Buffer exchange를 했을 때 cationic exchange resin인 CM-sepharose의 경우 pH 4.8에서 상대적으로 가장 높은 흡착율(77%)을 보였으며 anionic exchange resin인 DEAE-sepharose를 이용한 흡착에서는 pH 5.5에서 높은 흡착율(74%)을 보였다. 이러한 결과를 바탕으로 buffer exchange 없이 hGM-CSF가 secretion된 배지를 pH를 맞춘 후 좁은 범위에서의 흡착 실험을 수행하였다. 그 결과 pH 4.6에서 CM-sepharose를 이용했을 때 흡착율이 84%로 가장 좋았다. 이러한 결과를 이용하면 외래 단백질을 생산하는 식물세포 배양시에 가장 문제가 되는 protease에 의한 목적 단백질의 degradation을 해결할 수 있는 in situ adsorption이 가능하리라 사료된다.

  • PDF

Replacing Concentrate with Wheat Straw Treated with Urea Molasses and Ensiled with Manure: Effects on Ruminal Characteristics, In situ Digestion Kinetics and Nitrogen Metabolism of Nili-Ravi Buffalo Bulls

  • Hassan, Z.;Nisa, M.;Shahzad, M.A.;Sarwar, M.
    • Asian-Australasian Journal of Animal Sciences
    • /
    • 제24권8호
    • /
    • pp.1092-1099
    • /
    • 2011
  • To evaluate the effects of replacing concentrate with urea molasses treated fermented wheat straw (FWS) ensiled with cattle manure (CM) on ruminal characteristics, in situ digestion kinetics and nitrogen (N) metabolism was studied in Nili Ravi cannulated buffalo bulls in a $4{\times}4$ Latin Square Design. Wheat straw treated with urea (4%) and molasses (6%) was ensiled with cattle manure (CM) (70:30) and fermented for 40 days. Four iso-nitrogenous and iso-caloric diets were formulated. In the FWS0, FWS10, FWS20 and FWS30 diets 0, 10, 20 and 30% of the concentrate was replaced with FWS, respectively. Daily intake by bulls was restricted to 1.5% dry matter (DM) of body weight. Ruminal ammonia nitrogen concentration was greater (p<0.05) in bulls fed FWS diet than for those fed FWS0 diet at 3, 6, 9 and 12 h post-parandial. Bulls fed FWS 20 and FWS 30 diets had higher ruminal pH at 3 and 6 h post-parandial than bulls fed FWS10 and FWS0. Ruminal total volatile fatty acid (VFA) concentrations 3 h post-parandial were greater (p<0.05) in bulls fed FWS0 than those fed FWS diets. However ruminal VFA tended to increase at 6, 9 and 12 h post-parandial as the level of FWS increased. In situ ruminal DM and neutral detergent fiber (NDF) degradation, rates of disappearance and extent of digestion were higher (p<0.05) for bulls fed FWS30 diet than those fed FWS0. Ruminal DM and NDF lag time tended to decrease (p<0.05) as FWS concentration in the diet increased. Feed intake, nitrogen intake, N-balance and blood urea-N did not differ (p>0.05) in buffalo bulls fed different diets. Wheat straw treated with urea and molasses and ensiled with CM enhanced the nutritive value of wheat straw and improved nutrient utilization in buffalo bulls when up to 30% of the concentrate was replaced with FWS; no adverse effects on ruminal characteristics and nutrients digestibilities were detected.

Validation of Sea Surface Temperature (SST) from Satellite Passive Microwave Sensor (GPM/GMI) and Causes of SST Errors in the Northwest Pacific

  • Kim, Hee-Young;Park, Kyung-Ae;Chung, Sung-Rae;Baek, Seon-Kyun;Lee, Byung-Il;Shin, In-Chul;Chung, Chu-Yong;Kim, Jae-Gwan;Jung, Won-Chan
    • 대한원격탐사학회지
    • /
    • 제34권1호
    • /
    • pp.1-15
    • /
    • 2018
  • Passive microwave sea surface temperatures (SST) were validated in the Northwest Pacific using a total of 102,294 collocated matchup data between Global Precipitation Measurement (GPM) / GPM Microwave Sensor(GMI) data and oceanic in-situ temperature measurements from March 2014 to December 2016. A root-mean-square (RMS) error and a bias error of the GMI SST measurements were evaluated to $0.93^{\circ}C$ and $0.05^{\circ}C$, respectively. The SST differences between GMI and in-situ measurements were caused by various factors such as wind speed, columnar atmospheric water vapor, land contamination near coastline or islands. The GMI SSTs were found to be higher than the in-situ temperature measurements at low wind speed (<6 m/s) during the daytime. As the wind speed increased at night, SST errors showed positive bias. In addition, other factors, coming from atmospheric water vapor, sensitivity degradation at a low temperature range, and land contamination, also contributed to the errors. One of remarkable characteristics of the errors was their latitudinal dependence with large errors at high latitudes above $30^{\circ}N$. Seasonal characteristics revealed that the errors were most frequently observed in winter with a significant positive deviation. This implies that SST errors tend to be large under conditions of high wind speeds and low SSTs. Understanding of microwave SST errors in this study is anticipated to compensate less temporal capability of Infrared SSTs and to contribute to increase a satellite observation rate with time, especially in SST composite process.