• Journal of Animal Science and Technology
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• 제45권3호
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• pp.433-442
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• 2003

본 연구는 옥수수 알곡의 가공방법(통 옥수수, 분쇄, flake 3.8mm, 2.8mm 및 1.5mm)이 in vitro 상의 건물 소화율 및 in sacco 방법에 있어 반추위 내에서의 건물 분해율에 미치는 영향를 규명하기 위해 반추위에 캐뉼라(cannulae)가 장착된 홀스타인 건유우 3두를 공시축으로 하여 수행하였는데 그 결과를 요약하면 다음과 같다. 옥수수 가공형태별 in vitro 소화율은 in sacco 방법에서와 비슷한 경향을 보였다. 48시간 배양시에 1.5mm flake 및 분쇄한 옥수수가 각각 92.3, 91.2%로서 가장 높았다, 다음으로 2.8mm 및 3.8mm flake가 각각 83.9, 83.4%이였다. 특히, 통 옥수수는 가장 낮았으며, density가 낮은 통 옥수수가 높은 것보다 2배 이상 빠르게 분해되었다. In sacco 방법에 의한 건물 분해율은 48시간대에서 1.5mm flake, 분쇄 옥수수 및 2.8mm flake가 각각 94.4, 88.0 및 87.0%로서 가장 높았고, 3.8mm flake는 61.8%로서 이들보다 유의적으로 낮았다. Density가 낮은 통 옥수수가 높은 것보다 12시간대까지는 많이 분해되는 경향을 보였으나, 유의적인 차이가 없었고, 24, 48시간대에서는 유의하게 높아졌다. 본 연구의 결과를 종합하면, 옥수수를 분쇄하거나, flaking 처리를 하면 통 옥수수에 비하여 반추위 내에서 건물 분해율이 유의하게 증가되었다. 또한 flake의 가공정도를 증진시킬수록 건물 분해율이 유의하게 증가되었으며, 1.5 mm로 얇게 가공한 flake는 반추위 내에서 급속하게 분해되는 경향이었다. 한편, 같은 통 옥수수에서도 density가 낮은 것이 빠르게 분해되었다.

• Asian-Australasian Journal of Animal Sciences
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• 제16권9호
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• pp.1303-1308
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• 2003

Samples of eleven brown midrib (ICSU 96031, ICSU 93046, ICSU 96082, ICSU 96078, ICSU 96075, ICSU 95101, ICSU 96034, ICSU 96063, ICSU 45116, ICSA 93-3 and ICSA 3845 X 3816) and nine white midrib genotypes (ICSU 96050, ICSU 96030, ISU 95082, SSG 59-3, FSHI 93-1, FSHI 2219A X 3211, HC 171, ICSA 93-2 and ICSA 93-1) based on their phenotypic appearance were collected at 50 per cent flowering from the sorghum germplasm grown at Research farm of IGFRI, Jhansi. These genotypes were evaluated with respect to crude protein, fiber composition, in-sacco dry matter, OM, cell wall components disappearance/digestibility besides the fodder yield, total phenolic and availability index values. Brown midrib genotypes were lower (p<0.05) in NDF, ADF, cellulose and acid detergent lignin concentration than white midrib genotypes. Mean NDF, ADF, cellulose and lignin contents were 69.4, 42.1, 35.4 and 5.7% in brown mid rib vis-a vis 75.8, 47.5, 39.6 and 7.3% in white mid rib genotypes. Nonsignificant (p>0.05) differences were observed in dry matter, crude protein and organic matter contents between brown midrib and white midrib genotypes. Phenolic contents were significantly (p<0.05) lower in browm mid rib (0.2) than white mid rib (0.3%) sorghum. Brown midrib genotypes exhibited significantly (p<0.05) higher in-sacco DM, OM and CP disappearance than normal (white midrib) genotypes. The mean degradability of DM, OM and CP was 64.1, 62.6 and 79.6% in brown mid rib and 53.1, 54.0 and 76.6% in white mid rib genotypes, respectively. There were no significant (p>0.05) differences between genotypes in extent of fiber fraction degradability though in-sacco NDF and ADF degradability was more by 5 and 4 units, respectively in brown midrib genotypes vis-a-vis white midrib genotypes. Average fodder yield (green and dry g/plant) and availability index (%) values were significantly (p<0.05) higher for brown midrib (474.2, 129.8 and 80.4) genotypes than white midrib (375.0, 104.8 and 69.2) genotypes. Lignin contents had significant negative correlation with DM, OM, NDF and ADF degradability. The results of the study revealed that brown midrib genotypes are superior not only with regard to chemical entities and disappearance of DM and fiber fractions but also better in respect of fodder yield and availability index values. Thus, brown midrib sorghum strains may be useful in increasing digestibility, intake, feed efficiency and animal performance.

• Asian-Australasian Journal of Animal Sciences
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• 제13권8호
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• pp.1113-1120
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• 2000

Productivity, nutrient contents, in vitro gas production and in sacco degradability of different fractions and whole OPF were determined to assess the feeding value of OPF as a ruminant feed. An in vivo digestibility trial was also carried out using goat. Freshly harvested OPF was randomly collected, partitioned and weighed. An OPF from 21 years older palm weighed 13.4 kg and the annual fresh matter yield of petiole, leaflet and midrib was 46.5, 11.8 and 3.4 ton/ha, respectively. Leaflet contained 439, 926, 698, 501, 168, 196, 748 and 52 (g/kg) of dry matter (DM), organic matter (OM), neutral detergent fiber (NDF), acid detergent fiber (ADF), cellulose (CE), hemicellulose (HC), total carbohydrate (TC) and non fiber carbohydrate (NFC), respectively. Petiole contained lower (p<0.01) DM, CP and EE contents than leaflet. Organic matter, CE and TC contents were higher (p<0.01) in petiole compared to leaflet. Silica and lignin contents were highest (p<0.01) in midrib followed by leaflet, whole OPF and least in petiole. The Ca, P, Na, K and Mg contents (g/100 g DM) of leaflet were 0.529, 0.182, 0.039, 0.876, and 0.168, respectively. In vitro DM digestibility (g/100 g) at 48 h of leaflet, petiole and midrib was 32.7, 38.7 and 30.2, respectively. The in sacco DM degradation (g/100 g) at 48 h of leaflet was higher than that of whole OPF, petiole and midrib. The in vivo digestibility of DM, OM, CP and ADF of whole OPF was 52, 56, 43 and 26%, respectively. It can be concluded that leaflet is the most nutritious fraction of OPF and midrib is the least. The nutrient content and digestibility of the whole OPF showed that OPF could be an alternative roughage source for ruminant diets.

• Asian-Australasian Journal of Animal Sciences
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• 제5권4호
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• pp.635-641
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• 1992

The influence of alkaline hydrogen peroxide (AHP) and peracetic acid treatment on in sacco digestion of aspen was evaluated in three non-lactating ruminally cannulated Holstein cows fed a diet containing 90% forage and 10% concentrate on a DM basis. AHP treatment decreased lignin concentration by 40 to 60% resulting in increased concentrations of neutral detergent fiber (NDF), acid detergent fiber (ADF) and cellulose. Lignin concentrations in peracetic acid treated samples were less than 10% of values for control samples. In sacco disappearance rates of aspen DM, NDF, ADF and cellulose increased (p<.05) with AHP and peracetic acid treatment. Effective degradability of DM, NDF, ADF and cellulose were determined at a ruminal outflow rate of $.05h^{-1}$. Effective degradabilities of AHP treated aspen were approximately three-fold greater and peracetic acid treated samples five-fold greater than untreated control samples. For all parameters measured, peracetic acid treatment resulted in higher (p<.05) digestion coefficients than AHP treated aspen. Results demonstrate that peracetic acid or AHP treatment can enhance the nutritive value of aspen sawdust for ruminants to a level comparable to that reported for many forages.

• 한국근적외분광분석학회:학술대회논문집
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• 한국근적외분광분석학회 2001년도 NIR-2001
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• pp.1268-1268
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• 2001

The availability of in vivo and in sacco degradability values are limited because those methods require work with fistulated animals and are rather complicated, labour intensive and expensive. That is to say, the dynamics and logistics of the methodology result in considerable work, due to limitations on the amount of samples, number of bags that can be placed in an animal and different time intervals to perform kinetic studies. Therefore, a simpler method is necessary to estimate the degradation characteristics of the feed. In this way, near infrared reflectance spectroscopy has been used to predict degradation characteristics of forages. In other hand, the possibility of achieving successful transfer of spectra and equations between instruments is closely related. The objective of this study was to confirm the potential of NIR to optimize work conditions to avoid duplicated efforts in collaborative trials on animal feeds evaluation between research institutions. For this purpose, one set with forty hays and dehydrated forages samples from SERIDA and ten samples with the same characteristics from SIA, were be used to create a spectral database. A calibration was developed using samples from degradation essays made in SERIDA to predict dry matter and crude protein degradability. With the addition of five samples from SIA in original calibration set, the effect of different origin and location was compensated.

• Asian-Australasian Journal of Animal Sciences
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• 제5권1호
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• pp.19-24
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• 1992

Formula feed for fattening cattle ground through 2 mm screen was incubated in the rumen of sheep and goats to evaluate effects of ratio of concentrate to hay and kinds of hay in a ration on determining the degradability (dg) value of protein using in sacco technique. Following results were obtained: 1) Residual dry matter (DM) and crude protein (CP) of formula feed decreased as the time of incubation increased. Regression analyses showed that rates of degradation of DM and CP in the rumen were not the same when they were determined under feeding of rations with different percentages of concentrate. 2) Rate of passage of digesta from the rumen differed between feeding of Italian ryegrass hay ration and that of alfalfa hay ration, but was not influenced by the percentage of concentrate in a ration. 3) The dg value was different when it was estimated with results obtained from determinations under feeding of Italian ryegrass hay ration or that of alfalfa hay ration. The percentage of concentrate in a ration had no influence on the dg value of protein in formula feed.

• Asian-Australasian Journal of Animal Sciences
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• 제20권12호
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• pp.1849-1857
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• 2007

The experiment was carried out using fistulated multiparous Holstein Friesian crossbred (75% Holstein Friesian and 25% Red Sindhi) dairy cows in their dry period fed on untreated rice straw to evaluate the nutritive value of local protein feed resources using the in sacco method and in vitro pepsin-pancreatin digestion. Experimental feeds were cottonseed meal (CSM); soybean meal (SBM); dried brewery's grains (DBG); palm kernel meal (PSM); cassava hay (CH); leucaena leaf meal (LLM). Each feedstuff was weighed into duplicate nylon bags and incubated in each of the two rumen fistulated cows for 0, 2, 4, 8, 16, 24, and 48 h. Rumen feed residues from bags of 16 h incubation were used for estimation of lower gut digestibility by the technique of in vitro pepsin-pancreatin digestion. Ruminal ammonia-nitrogen ($NH_3-N$) concentrations did not differ between treatments or time with a mean of 5.5 mg%. Effective degradability of DM of CSM, SBM, DBG, PSM, CH and LLM were 41.9, 56.1, 30.8, 47.0, 41.1 and 47.5%, respectively. Effective degradabilities of the CP in feedstuffs were 49.6, 59.2, 40.9, 33.5, 47.3 and 65.0% for the respective feedstuffs. The CP in vitro pepsin-pancreatin digestibility as ranked from the highest to the lowest were SBM, CSM, LLM, CH, DBG, PSM, respectively. The intestinal and total tract digestion of feedstuffs in the current study were relatively lower than that obtained from previous literature. The results of this study indicate that SBM and LLM were highly degradable in the rumen, while CH, CSM and DBG were less degradable and, hence resulted in higher rumen undegradable protein. Soybean meal and LLM could be used to improve rumen ecology whilst CH, CSM and DBG could be used as rumen by-pass protein for ruminant feeding in the tropics.

• Asian-Australasian Journal of Animal Sciences
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• 제16권3호
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• pp.368-373
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• 2003

This experiment was conducted to examine the effects on the composition and rumen degradation in sacco of rice straw treated with animal urine (1 l of 2.9 g N/kg DM straw) and urea plus calcium hydroxide (2% urea plus 0.5% $Ca(OH)_2$/kg DM straw) as a cheap and relatively safe alternative for ammonia (3% ammonia solution/kg DM straw). Mold occurred in urine treated straw, but other treatments were apparently mold-free. All treatments significantly (p<0.05) increased CP content in the straw compared with untreated one. Ammonia-treated straw contained CP at about twice that in urine or urea-calcium hydroxide treated straw. NDF and hemicellulose contents decreased significantly (p<0.05) in all treatments, while ADF and cellulose showed no differences compared with untreated straw. The degradable fraction of DM, CP, NDF, hemicellulose and cellulose was significantly (p<0.05) increased for ammonia and urea-calcium hydroxide treatments than for urine treated or untreated straw except for CP of urine treated straw. Chemical treatment of rice straw increased the readily degradable fraction of CP, while it decreased the slowly degradable fraction for urine or urea-calcium hydroxide treated rice straw. The degradation rate of hemicellulose was significantly (p<0.05) increased for ammonia and urea-calcium hydroxide treatments compared to urine treated or untreated straw. However, no effect on cellulose degradation rate was found by any of the treatments. There was no improvement in the degradation kinetics caused by the urine treatment despite the improvement of the chemical composition. Although the improvement in rumen degradability was less in the urea-calcium hydroxide treatment than in the ammonia treatment, its use may be more desirable because it is less expensive to obtain, less hazardous nature, and readily available. For further improvement it is necessary to investigate the supplementation of slowly degradable nitrogen to ureacalcium hydroxide treated rice straw diet.

• Asian-Australasian Journal of Animal Sciences
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• 제12권8호
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• pp.1222-1227
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• 1999

Effect of three major cropping seasons and five fertilizer treatments on botanical composition, nutritional composition and in sacco digestibility of native grasses grown in 30 experimental plots of a medium fertile land was determined. It was observed that all the major grass species were grown in all seasons but their predominancy of growth was different. During the study the predominant grass species were Panicum repens (Angta), Fimvristylis miliacea (Joina), Cyanolis axillaries (Kanainala), Cynodon dactylon (Durba) and Cyperus iria (Phulchaise) which contributed about 27, 20, 13, 11 and 9% of the total grass yield, respectively. Dry matter (DM) contents was higher in dry followed by monsoon and summer seasons (p<0.05). Crude protein (CP) content in the summer and monsoon appeared to be higher (p<0.05) than that of dry season. Organic matter (OM) and neutral detergent fibre (NDF) were higher (p<0.05) in dry and monsoon than in summer season. Application of urea fertilizer and cowdung increased 28.2% of CP content of the grasses, but decreased 19.5 and 9.8% of DM and NDF contents, respectively. The potential degradation of DM and CP of the grasses grown in summer were 4.1 and 8.4% and 3.9 and 5.8% higher than those of monsoon and dry seasons, respectively, and both of these increased (11.3 and 5.9%, respectively) with the application of cowdung and urea fertilizer.

• Asian-Australasian Journal of Animal Sciences
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• 제20권2호
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• pp.220-228
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• 2007

The nutritive value and the effect of tannins on the utilization of foliage from three commonly used legumes, Acacia nilotica, Albizia procera, and Sesbania acculeata, were determined. Three mature rumen-fistulated bullocks were used to study in sacco degradability and twelve adult sheep were randomly allocated on the basis of live weight to 4 groups of 3 in each to study the in vivo digestibility of the foliages. In all foliages, the contents of crude protein (17 to 24% of DM) were high. Fibre was especially high in Albizia (NDF 58.8% of DM vs. 21% in Sesbania and 15.4% in Acacia). Contents of both hydrolysable (4.4 to 0.05%) and condensed tannins (1.2 to 0.04%) varied from medium to low in the foliages. Acacia contained the highest level of total phenolics (20.1%), protein precipitable phenolics (13.2%) and had the highest capacity to precipitate protein (14.7%). Drying in shade reduced the tannin content in Acacia and Albizia by 48.6 and 69.3% respectively. The foliages ranked similarly for each of the different methods used to estimate tannin content and activity. Acacia and Sesbania foliage was highly degradable (85-87% potential degradability of DM in sacco), compared to Albizia (52%), indicating a minimal effect of tannins in Acacia and Sesbania. Yet, in vitro, the tannins in the Acacia inhibited microbial activity more than those in Albizia and Sesbania. Following the addition of polyethylene glycol to neutralise the tannins, gas production and microbial growth increased by 59% and 0.09 mg RNA equiv./dg microbial yield respectively in the Acacia, compared to 16-17% and 0.06 mg RNA equiv./dg microbial yield in the other foliages. There was a trend for low in vivo apparent digestibility of N in the Acacia (43.2%) and Albizia (44.2%) compared to the Sesbania (54.5%) supplemented groups. This was likely to be due to presence of tannins. Consistent with this was the low N retention (0.22 and 0.19 g N/g NI) in sheep supplemented with Acacia and Albizia compared to that for the Sesbania (0.32). Similarly, a trend for poor microbial N yield was observed in sheep fed these foliages. Across the foliages tested, an increase in tannin content was associated with a reduction in ruminal fermentation, N digestibility and N retention. For overall nutritive value, Sesbania proved to be the superior forage of the three tested.