• Journal of Life Science
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• v.28 no.3
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• pp.307-313
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• 2018

Alopecia cause psychological stress due to their effect on appearance. Thus, the global market size of the alopecia treatment products are growing quickly. Timosaponin A III is the well known active ingredient of Anemarrhenae Rhizoma. In this study, we investigated and compared the binding affinity of timosaponin A III with finasteride (5-beta reductase antagonist) and minoxidil (androgen receptor antagonist) on the target protein active site by in silico computational docking studies. The three dimensional crystallographic structure of 5-beta reductase (PDB ID : 3G1R) and androgen receptor (PDB ID: 4K7A) was obtained from PDB database. In silico computational autodocking analysis was performed using PyRx, Autodock Vina, Discovery Studio Version 4.5, and NX-QuickPharm option based on scoring functions. The timosaponin A III showed optimum binding affinity (docking energy) with 5-beta reductase as -12.20 kcal/mol as compared to the finasteride (-11.70 kcal/mol) and with androgen receptor as -9.00 kcal/mol as compared to the minoxidil (-7.40 kcal/mol). The centroid X, Y, Z grid position of the timosaponin A III on the 5-beta reductase was similar (overlap) to the finasteride, but the X, Y, Z centroid grid of the timosaponin A III on the androgen receptor was significantly far from the minoxidil centroid position. These results significantly indicated that timosaponin A III could be more potent antagonist to the 5-beta reductase and androgen receptor. Therefore, the extract of Anemarrhenae Rhizoma or timosaponin A III containing biomaterials can substitute the finasteride and minoxidil and can be applied to the alopecia protecting product and related industrial fields.

• Journal of the Computational Structural Engineering Institute of Korea
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• v.27 no.6
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• pp.625-633
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• 2014

Mark III CCS plate is considered in this paper to perform its strength assessment. Mark III CCS plate is designed and constructed by stacking various non-metallic engineering materials such as plywood, triplex and reinforced PU foam that are supported by series of mastic upon inner steel hull structure. From the viewpoint of structural analysis, this plated structure is treated as a laminated anisotropic structure. Commercially available general purpose finite element analysis programs such as MSC PATRAN and MARC are used to develop the finite element (FE) model of the Mark III CCS plate. Because of the characteristics of LNG cargo that the Mark III CCS plate deals with, it is subjected to a wide range of temperature variations, i.e. about $-163^{\circ}C$ to $20^{\circ}C$. Different material properties of the Mark III CCS plate at these temperature levels are considered in the FE model. Using the developed FE model, strength assessment procedure is developed incorporating various anisotropic failure criteria such as Hashin, Hill, Hoffman, Maximum stress and Tsai-Wu. The strength assessment is performed within the initial failure state of the Mark III CCS plate and, as a result, failure details such as failure locations and loads are identified.

• Analytical Science and Technology
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• v.8 no.4
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• pp.499-503
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• 1995

In order to study how and why the stabilities of lanthanoid(III) complexes in solutions vary across the series, the formation constants of the adducts of tris(2-thenoyltrifluoroacetonato)lanthanoids(III) with seven carboxylic acids in chloroform have been determined by solvent extraction technique at 298K. The formation constants with carboxylic acids generally decrease with increasing the atomic number, but in the middle of the series, they change only slightly. Such trends have been interpreted as related to a change of the coordination number in the middle of the series. It has been attempted to determine the number of water molecules coordinated to the adducts as well as $Eu(TTA)_3$ in chloroform by measuring the fluorescence life time of europium(III), to ensure the assignment of the coordination number.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• v.26 no.1
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• pp.1-4
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• 2000

True midfacial deficiency is defined as a hypoplasia of various components of midface such as maxilla, orbit, zygoma and nasal bone. For treatment of these anomalies Le Fort III osteotomy and its modifications have been used traditionally. Le Fort III osteotomy is the method which advances maxilla with nasal bone and zygomatic bone at a time. At first midfacial osteotomy was introduced by Gillies to treatment of dentofacial deformity in 1950. In 1967 Tessier designed Le Fort III osteotomy according to Le Fort III midfacial fracture line and popularized to treat midfacial deficiency using coronal incision to appoach osteotomy sites. This is a case of patient who had mandibular prognathism with midfacial deficiency with severe discrepancy in maxillomandibular interrelation. First we performed Le Fort III osteomomy for zygomaticomaxillary advancement, and then carried out simultaneous two jaw surgery with Le Fort I osteotomy and BSSRO three months after first surgery.

• BMB Reports
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• v.40 no.5
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• pp.783-790
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• 2007

Receptor binding plays an important role in determining host specificity of the Bacillus thuringiensis Cry $\delta$-endotoxins. Mutations in domains II and III have suggested the participation of certain residues in receptor recognition and insect specificity. In the present study, we expressed the cloned domain II-III fragment of Cry4Ba and examined its binding characteristics to mosquito-larval midgut proteins. The 43-kDa Cry4Ba-domain II-III protein over-expressed in Escherichia coli as inclusion bodies was only soluble when carbonate buffer, pH 10.0 was supplemented with 4M urea. After renaturation via stepwise dialysis and subsequent purification, the refolded domain II-III protein, which specifically reacts with anti Cry4Ba-domain III monoclonal antibody, predominantly exists as a $\beta$-sheet structure determined by circular dichroism spectroscopy. In vitro binding analysis to both histological midgut tissue sections and brush border membrane proteins prepared from susceptible Aedes aegypti mosquito-larvae revealed that the isolated Cry4Ba-domain II-III protein showed binding functionality comparable to the 65-kDa full-length active toxin. Altogether, the data present the 43-kDa Cry4Ba fragment comprising domains II and III that was produced in isolation was able to retain its receptor-binding characteristics to the target larval midgut proteins.

• Food Science and Biotechnology
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• v.14 no.4
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• pp.551-553
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• 2005

Carbonic anhydrase III was found in liver of flounder, Limanda yokohamae. Protein was isolated from cytosolic extracts and identified using SDS-PAGE and isolectric focusing. Specific protein bands with molecular weight of 30 kDa and pIs of 7.0 and 6.5 were detected by Western blotting. This is the first report of identification of carbonic anhydrase III from L. yokohamae.

• Bulletin of the Korean Chemical Society
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• v.26 no.9
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• pp.1453-1456
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• 2005

• The korean journal of orthodontics
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• v.20 no.1
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• pp.123-133
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• 1990

The work was undertaken to evaluate the calcification of the second and the third molars in skeletal Class II and III malocclusions. The differences in the calcification stages between skeletal Class II and III malocclusion were evaluated and statistically analysed from panoramic radiographs of 202 males and females ranging in age from 11 to 15 years old. The results were as follows, 1. The calcification stages of the second and the third molars were not different between the skeletal Class II and III malocclusions in each age groups of both sexes. 2. The calcification stages of lower second and third molars of the skeletal Class III malocclusion are more advanced than those of the skeletal Class II malocclusion in male. 3. The clacification stages of upper second and third molars are more advanced than those of lower second and third molars in skeletal Class II malocclusion. 4. The calcification stages of lower second and third molars are more advanced than those of upper second and third molars in skeletal Class III malocclusion.

• Journal of mucopolysaccharidosis and rare diseases
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• v.2 no.1
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• pp.5-7
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• 2016

Mucolipidoses II and III alpha/beta (ML II and ML III) are lysosomal disorders in which the essential mannose-6-phosphate recognition marker is not synthesized onto lysosomal hydrolases and other glycoproteins. The disorders are caused by mutations in GNPTAB, which encodes two of three subunits of the heterohexameric enzyme, N-acetylglucosamine-1-phosphotransferase ML II, recognizable at birth, often causes intrauterine growth impairment and sometimes the prenatal "Pacman" dysplasia. The main postnatal manifestations of ML II include gradual coarsening of neonatally evident craniofacial features, early cessation of statural growth and neuromotor development, dysostosis multiplex and major morbidity by hardening of soft connective tissue about the joints and in the cardiac valves. Fatal outcome occurs often before or in early childhood. ML III with clinical onset rarely detectable before three years of age, progresses slowly with gradual coarsening of the facial features, growth deficiency, dysostosis multiplex, restriction of movement in all joints before or from adolescence, painful gait impairment by prominent hip disease. Cognitive handicap remains minor or absent even in the adult, often wheelchair-bound patient with variable though significantly reduced life expectancy. As yet, there is no cure for individuals affected by these diseases. So, clinical manifestations and conservative treatment is important. This review aimed to highlight the extra-skeletal clinical problems in ML II and III.

• Journal of Korean Academy of Nursing
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• v.34 no.2
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• pp.278-289
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• 2004

Purpose: The purpose of this study was to classify the elderly in long-term care facilities using the Resource Utilization Group(RUG-III) and to examine the feasibility of a payment method based on the RUG-III classification system in Korea. Method: This study measured resident characteristics using a Resident Assessment Instrument-Minimum Data Set(RAI-MDS) and staff time. Data was collected from 530 elderly residents over sixty, residing in long-term care facilities. Resource use for individual patients was measured by a wage-weighted sum of staff time and the total time spent with the patient by nurses, aides, and physiotherapists. Result: The subjects were classified into 4 groups out of 7 major groups. The group of Clinically Complex was the largest (46.3%), and then Reduced Physical Function(27.2%), Behavior Problems (17.0%), and Impaired Cognition (9.4%) followed. Homogeneity of the RUG-III groups was examined by total coefficient of variation of resource use. The results showed homogeneity of resource use within RUG-III groups. Also, the difference in resource use among RUG major groups was statistically significant (p<0.001), and it also showed a hierarchy pattern as resource use increases in the same RUG group with an increase of severity levels(ADL). Conclusion: The results of this study showed that the RUG-Ill classification system differentiates resources provided to elderly in long-term care facilities in Korea.