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Study on suitable substrate formulation for bottle and bag cultivation of Pleurotus pulmonarius (산느타리(Pleurotus pulmonarius) 병·봉지재배에 적합한 배지조성 연구)

  • Lee, Jae-Hong;Lee, Nam-Gil;Park, Young-Hak;Mun, Youn-Gi;Jeong, Tae-Sung;Kwon, Sun-Bae;Kim, Jae-Rok;Kim, Jin-Won
    • Journal of Mushroom
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    • v.12 no.4
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    • pp.275-279
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    • 2014
  • Pleurotus pulmonarius usually grows on the dead wood of deciduous trees from mid summer to early fall. Cultivation of this mushroom is recently increasing due to its good flavour and fragrance. This study was conducted to find out the favorable substrate formulation in the bottle and bag cultivation of P. pulmonarius. The V formulation (10 : 50 : 20 : 20 mixing ratio of poplar sawdust, cottonseed hull, cottonseed meal and beet pulp) showed the fastest mycelial growth in the test tube column medium and highest yields in both bottle cultivation and bag cultivation. Those chemical conditions were pH 5.52, 29.07% organic matter contents, 1.12% total nitrogen, 25.95 OM/N ratio, 0.16% CaO, 0.51% $K_2O$, 0.17 MgO and 0.26 $P_2O_5$.

Clinical Analysis Of Ventricular Septal Defect (심실중격결손증의 외과적 고찰)

  • Seong, Suk-Hwan;Suh, Kyung-Pill
    • Journal of Chest Surgery
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    • v.15 no.1
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    • pp.90-97
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    • 1982
  • Two hundred one patients of ventricular septal defect, which were operated at Seoul National University Hospital, were analysed on clinical background during the period from January, 1975 to December, 1980. The results were as follows: 1. Of the 201 patients, 118 patients were male [58.7%] and 83 patients were female [41.3%]. Their age ranged from 15 months to 40 years, and the mean age was 8.7 years. 40% of the patients were between 4 and 8 years. 2. The most common symptoms showed frequent U RI and exertional dyspnea. 3. On Kirklin`s anatomical classification, type I constituted 26.9%, type II 58.2%, type III 12.4%, and type IV 1.0%. We showed marked increased incidence of type I VSD as compared to Caucasians* 4. 46 cases were associated with other congenital cardiac diseases. They were PDA [13 cases], AI[11 ], ASD[6], PS[10], MI[4], and Double aortic arch [1]. 5. In 128 patients, who had complete hemodynamic data and were not associated with other congenital cardiac diseases, an attempt was made to correlate the EKG findings with the hemodynamic data, and defect size with the hemodynamic data. The children had variable distribution of PA syst. pr. and Rp/Rs. But most of adults had $R_P$/$R_S$of 0.15 or less. As $P_P$/$P_S$increased, the rate of operative complication increased also. 6. When a normal EKG pattern was present, $Q_P$/$Q_S$and $R_P$/$R_S$and $P_P$/$P_S$were relatively low. When EKG findings were LVH pattern, there was diastolic volume overload to left ventricle. As RVH, there was systolic pressure overload to right ventricle. And as BVH, there was mixed pattern of diastolic volume overload to left ventricle and systolic pressure overload to right ventricle. 7. Among patients in defect was less than 1 $cm^2$ per $M^2$ of BSA, $Q_P$/$Q_S$was less than 2:1, and $R_P$/$R_S$less than 0.25, and PAsyst. pr. less than 50 mmHg, and $P_P$/$P_S$was less than 0.5. But patients with the defect greater than 1 $cm^2$ per $M^2$ of BSA had no correlationship between $Q_P$/$Q_S$, $R_P$/$R_S$, PAsyst. pr. and defect size in each other. Most of patients with the defect greater than 2 $cm^2$/$M^2$ BSA, $R_P$/$R_S$was greater than 0.5. 8. Operative mortality rate was 9.5% [19 cases] among 201 patients. And complication rate including mortality rate was 22.9% [46 cases].

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Stratigraphy and Paleoenvironment of Domi-1 and Sora-1 Wells, Domi Basin (도미분지 도미-1, 소라-1공의 층서와 고환경)

  • Yun, Hye-Su;Byun, Hyun-Suk;Oh, Jin-Yong;Park, Myong-Ho;Lee, Min-Woo
    • Economic and Environmental Geology
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    • v.45 no.4
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    • pp.407-429
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    • 2012
  • There has been much debates on the geologic age of the sediments of the Domi Basin, since age results varied after research methods and fossil groups. This study carried out palynological analysis and seismic interpretation to establish a stratigraphy and environmental reconstruction mainly based on fossil dinoflagellates and Seismic data from the Domi-1 and Sora-1 wells. The dinocyst assemblages found enabled zonation of the well sediment sequence resulting in 4 ecozones. Index fossils among dinocysts and palynomorphic substances indicate geologic age of the well ranges from Eocene to Pleistocene, and paleoenvironment varies from freshwater to inner-neritic marine. The fossil association also suggests strong relationship to Japanese Tertiary basins in Kyushu area in terms of stratigraphy and basin developmental history.

Transcriptional regulation of soybean ${\beta}-conglycinin$ gene expression. -(I) Identification of a soybean embryo factor interacting with upstream region of soybean ${\beta}-conglycinin$ gene- (대두 ${\beta}-conglycinin$ 유전자 발현의 전사 조절에 관한 연구 -(I) 대두 ${\beta}-conglycinin$ 유전자의 upstream 영역에 결합하는 대두 배 인자의 동정-)

  • Lee, Jeong-Yeon;Chung, Dong-Hyo;Kim, Woo-Yeon
    • Applied Biological Chemistry
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    • v.36 no.6
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    • pp.547-552
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    • 1993
  • Soybean nuclear extracts and S-100 were prepared to examine the soybean embryo factors which bind to the upstream region of soybean ${\beta}-conglycinin$ ${\alpha}'$ subunit gene. SEF3(soybean embryo factor 3), which is presumed to be a trans-acting factor for the expression of the gene, was detected in gel mobility shift assay using the DNA probe containing two AACCCA hexanucleotides. DNA probe containing CATGCAT or AACACA was used to find any other soybean embryo factor interacting with the upstream region of ${\beta}-Conglycinin$ ${\alpha}'$ subunit gene. It was found that there was no common DNA binding protein detected both in nuclear extracts and S-100. The relative levels of SEF3 binding activity both in nuclear extracts and S-100 of maturing soybean seeds were determined. SEF3 activity of nuclear extracts was first detected around 20 days after pollination and significantly increased around 32 days after pollination.

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A Study on the Status and Supporting Strategy of National R&D Programs related to the Convergence Technology (융합기술 관련 국가 연구개발 사업 현황과 효과적 지원전략에 대한 연구)

  • Kim, Yun-Jong;Jung, Uk;Jeong, Sang-Ki
    • Journal of Korea Technology Innovation Society
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    • v.12 no.2
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    • pp.413-429
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    • 2009
  • As science and technology which the society requires become highly advanced and complicated, the need of so-called Convergence Technology(CovT) is getting more significant. The major developed countries are presenting their own vision and strategies of CovT to take the lead of future society and global economies. In Korea, each ministries and government offices are suggesting their own directions of promoting CovT and supporting the related programs. This paper investigates the overall status of national and international R&D activities related to CovT in order to invigorate the related national R&D programs and maximize their effectiveness. It also purports to realize the growth potential and a ripple effect of CovT, such as new business creation and fulfillment of public needs, so that secure new CovT in an early stage. Based on the analysis of the overall status of national R&D programs, we redefine and re-categorize the CovT, which is especially oriented to the development of government support policy, and present the customized strategies to each categories.

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A Study of Vertical Axis Wind Turbine by Simulation (시뮬레이션을 통한 수직축 풍력발전에 관한 연구)

  • Park, Jung-Cheul;Won, Woo-Yeon
    • The Journal of Korea Institute of Information, Electronics, and Communication Technology
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    • v.11 no.3
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    • pp.241-245
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    • 2018
  • This paper designed the main blade in V-shape and tried to identify the best design conditions by changing the main blade angle and wind speed. When the main blade open angle was maintained at $120^{\circ}$ and the sample2 with an angle changed by $30^{\circ}$ was compared with the sample3 changed by $60^{\circ}$, the power output of sample2 was 3.8[kW], the power coefficient was 0.12, and the power output of sample3 was 6.0[kW], the power coefficient was measured as 0.18. So the power output of sample 3 was 58% higher than that of sample2, and the power coefficient was increased by 50%. The power coefficient was 0.18 and the wind speed was changed to 10[m/s] at 6.0[kW] at wind speed of 7[m/s] by fixing main blade open angle of $120^{\circ}$ and angle of $60^{\circ}$. The output was measured at 7.7[kW] and the power coefficient at 0.23. When the wind speed was high, the power output increased by 28% and the power coefficient increased by 83%. Also, sample4 was 103% higher in output and 92% more in efficiency than sample2.

The Changes of Quality of Life in Cancer Patients Treated with Chemotherapy (화학치료를 받는 암 환자 '삶의 질'의 변화에 관한 연구)

  • Kim, Byung-Soo;Choi, In-Keun;Park, Kyeung-Hwa;Yoon, So-Young;Oh, Sang-Chul;Seo, Jae-Hong;Choi, Chul-Won;Shin, Sang-Won;Kim, Yeul-Hong;Kim, Jun-Suk
    • Journal of Hospice and Palliative Care
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    • v.4 no.2
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    • pp.130-136
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    • 2001
  • Purpose : It is very important to endow the cancer patients underwent chemotherapy with satisfactory quality of life (QOL). However, little is known about the factors influencing QOL during chemotherapy. Therefore, we designed this study to find out the factors influencing QOL in the cancer patients who underwent chemotherapy. Methods : Ninety-seven cancer patients were studied, prospectively. The patients' characteristics were as follows; median age(range): 48(19{\sim}83) years, male:female; 57:40, PS:0,1/2,3;55/42 patients, diagnosis(number): lymphoma (28), lung cancer (22), gastrointestinal cancer (18), sarcoma (12), breast cancer (12), gynecological cancer (5), Stage: I,II/III.IV;37/60 patients. We used EORTC QLQ-C30 questionnaires to evaluate QOL. EORTC QLQ-C30 scores were performed before the onset of chemotherapy and after the end of 3 cycles of chemotherapy. The correlation of these scores with performance status (PS), diagnosis, disease stage, response to chemotherapy, and regimen related toxicity was evaluated. Results : The responder group (CR, PR) demonstrated marked improvement of social functional and emotional scales to non-responder group (SD,PD) (P=0.024, 0.045). Non-hematologic regimen related toxicity such as mucositis, nausea and vomiting was significantly correlated with pain scale change (P=0.043). Other factors had no notable correlation with QOL changes. Conclusion : Our preliminary study results may suggest as follows. The response to chemotherapy is associated with the change of social functional and emotional scales and the severity of non-hematologic regimen related toxicity is associated with pain scale change.

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Cell Surface Display of Arylsulfatase Gene from Pseudoalteromonas carageenovora in Saccharomyces cerevisiae (Saccharomyces cerevisiae에서 Pseudoalteromonas carageenovora 유래 Arylsulfatase 유전자의 표층 발현)

  • Cho, Eun-Soo;Kim, Hyun-Jin;Jung, So-A;Kim, Jeong-Hwan;Kim, Yeon-Hee;Nam, Soo-Wan
    • Microbiology and Biotechnology Letters
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    • v.37 no.4
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    • pp.355-360
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    • 2009
  • In this study, the arylsulfatase gene (astA, 984 bp ORF) from Pseudoalteromonas carrageenovora genome was expressed on the cell surface of S. cerevisiae by fusing with Aga2p linked to the membrane anchored protein, Aga1p. The constructed plasmid, pCTAST (7.1 kb), was introduced to S. cerevisiae EBY100 cell, and yeast transformants on YPDG plate showed the hydrolyzing activity for 4-methylumbelliferyl-sulfate and p-nitrophenyl-sulfate. When S. cerevisiae EBY100/pCTAST was grown on YPDG medium, the arylsulfatase activity of cell pellet reached about 1.2 unit/mL, whereas no extracellular arylsulfatase activity was detected. The DNA ladder in agarose prepared from agar by this recombinant arylsulfatase showed similar resolution and migration patterns with a commercial agarose. This results revealed that arylsulfatase expressed on the cell surface of S. cerevisiae could be applicable to the economic production of electrophoretic-grade agarose.

Pre-treatment Metabolic Tumor Volume and Total Lesion Glycolysis are Useful Prognostic Factors for Esophageal Squamous Cell Cancer Patients

  • Li, Yi-Min;Lin, Qin;Zhao, Long;Wang, Li-Chen;Sun, Long;Dai, Ming-Ming;Luo, Zuo-Ming;Zheng, Hua;Wu, Hua
    • Asian Pacific Journal of Cancer Prevention
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    • v.15 no.3
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    • pp.1369-1373
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    • 2014
  • Objectives: To study application of the maximum standardized uptake value (SUVmax), metabolic tumor volume (MTV) and total lesion glycolysis (TLG) with $^{18}F$-FDG PET/CT for predicting prognosis of esophageal squamous cell cancer (ESC) patients. Methods: Eighty-six patients with ESC staged from I to IV were prospectively enrolled. Cisplatin-based chemoradiotherapy (CCRT) or palliative chemoradiotherapy were the main treatment methods and none received surgery. $^{18}F$-FDG PET/CT scans were performed before the treatment. SUVmax, MTV, and TLG were measured for the primary esophageal lesion and regional lymph nodes. Receiver operating characteristic curves (ROCs) were generated to calculate the P value of the predictive ability and the optimal threshold. Results: MTV and TLG proved to be good indexes in the prediction of outcome for the ESC patients. An MTV value of 15.6 ml and a TLG value of 183.5 were optimal threshold to predict the overall survival (OS). The areas under the curve (AUC) for MTV and TLG were 0.74 and 0.70, respectively. Kaplan-Meier analysis showed an MTV less than 15.6 ml and a TLG less than 183.5 to indicate good media survival time (p value <0.05). In the stage III-IV patient group, MTV could better predict the OS (P < 0.001), with a sensitivity and specificity of 0.80 and 0.67, respectively. Conclusions: Pre-treatment MTV and TLG are useful prognostic factors in nonsurgical ESC.

Evaluation of Health-related Quality of Life for Hypothesized Medical States Associated with Cervical Cancer

  • Murasawa, Hideki;Konno, Ryo;Okubo, Ichiro;Arakawa, Ichiro
    • Asian Pacific Journal of Cancer Prevention
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    • v.15 no.22
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    • pp.9679-9685
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    • 2014
  • Background: When evaluating health-economics for cervical cancer prevention policies in Japan, it is important to use Japanese value settings. This study aimed to obtain preference-based measures (preference measures) for hypothesized health states among healthy Japanese women, and to examine differences between the EuroQol-5D (EQ-5D) and standard gamble (SG) instruments. Materials and Methods: The investigation was performed among female students at a nursing university. We used written hypothetical scenarios describing three grades of cervical intraepithelial neoplasia (CIN) and eight stages of cervical cancer, both at diagnosis and after medical intervention. Preference measures were evaluated using both EQ-5D and SG. Results: We received responses from 136 women. The mean number of respondents per stage was 24.6 (SD: 2.7). At diagnosis, average EQ-5D scores for CIN1, CIN2, CIN3, IA1, IA2, IB1, IB2, IIA, IIB, III, and IV stages were 0.84 (0.14), 0.78 (0.12), 0.73 (0.10), 0.78 (0.12), 0.72 (0.12), 0.63 (0.13), 0.64 (0.12), 0.68 (0.08), 0.62 (0.13), 0.55 (0.21), and 0.18 (0.24), respectively. Using one-way analysis of variance with the Tukey-Kramer method for multiple comparisons (each stage vs. CIN1), we found significant differences for IB1 and more advanced stages (p<0.05). After medical intervention, corresponding EQ-5D scores were 0.84 (0.12), 0.81 (0.12), 0.84 (0.12), 0.80 (0.15), 0.78 (0.11), 0.64 (0.15), 0.63 (0.15), 0.71 (0.15), 0.50 (0.17), 0.52 (0.17), 0.21 (0.28). The multiple comparisons identified significant differences for IB1 and more advanced stages, excepting IIA (p<0.05). SG evaluations were more variable and relatively higher than EQ-5D evaluations. Conclusions: We obtained preference measures for three grades of CIN1-3 and eight stages of cervical cancer. In combination with appropriate sensitivity analyses, these preference measures will provide a basis for an economic evaluation of cervical cancer prevention in Japan. We suggest that EQ-5D is appropriate for cost-utility analysis of this topic.