• Title/Summary/Keyword: Improvements

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Molecular Breeding of Genes, Pathways and Genomes by DNA Shuffing

  • Stemmer, Willem P.C.
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.7 no.3
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    • pp.121-129
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    • 2002
  • Existing methods for optimization of sequences by random mutagenesis generate libraries with a small number of mostly deleterious mutations, resulting in libraries containing a large fraction of non-functional clones that explore only a small part of sequence space. Large numbers of clones need to be screened to find the rare mutants with improvements. Library display formats are useful to screen very large libraries but impose screening limitations that limit the value of this approach for most commercial applications. By contrast, in both classical breeding and in DNA shuffling, natural diversity is permutated by homologous recombination, generating libraries of very high quality, from which improved clones can be identified with a small number of complex screens. Given that this small number of screens can be performed under the conditions of actual use of the product, commercially relevant improvements can be reliably obtained.

A Study on the Improvements for the Legal Systems Related to the Coneservation of Mountain Ridge Areas - In Case of Hwasung - (능선부 산지보전 관련 제도 개선에 관한 연구 - 화성시를 사례로 -)

  • Choi, Hyung-Seok
    • Journal of the Korean Society of Environmental Restoration Technology
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    • v.12 no.5
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    • pp.133-144
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    • 2009
  • This study intends to suggest the improvements for the legal systems related to the conservation of mountain ridge areas. The 4 legal systems directly related to the conservation of mountain ridge areas are reviewed and the current conditions of adaptive reuse of mountain areas and destroy cases in Hwasung are examined. For solving the problems on the basis of the analysis, three proposals for the improvement on legal systems are suggested. First, the integration of present dual legal systems related to adaptive reuse of mountain areas on the assumption that the related criteria and provisions should be amended, second, activation of the legal systems such as natural landscape district and natural landscape review to make up for the laws related to adaptive reuse of mountain areas, third, the enactment and application of the ordinances related with the 'mountain area management law'.