• 한국식품과학회지
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• 제25권1호
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• pp.78-82
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• 1993

이 연구는 재래방법과 비교하여 수율이 높고 품질이 향상된 효모 자가분해들의 제조방법을 연구하는데 그 목적을 두었다. 제빵용 생 효모에 glucanase와 protease의 복합효소를 작용시킨 다음, 10,000 psi의 고압으로 균질하면 약 85.3%의 효모 세포벽이 파손되었다. 세포벽이 파손된 효모를 사용하여 자가분해 시키는데 가장 적당한 조건을 반응 표변방법(RSM)을 이용하여 조사한 결과는 pH 7.0, 온도 $30^{\circ}C$ 에탄올 4%, 소금 3%이었다. 세포벽이 파손된 효모를 위와 같은 최적조건에서 3일간 분해시켜 얻은 효모 자가분해물의 수율은 2.6배 향상되었으며, 총 아미노산의 함량도 현저히 증가(2.8배)하였다. 그리고 관능적인 품질도 재래방법으로 제조된 시료보다 고기 향미가 많고, 이미-이취가 현저하게 적어서 전체인 기호도에서도 상당히 우수한 것으로 평가되었다.

• Journal of Periodontal and Implant Science
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• 제38권sup2호
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• pp.373-384
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• 2008

Purpose: The aim of this study is to compare the healing response of various Hydroxyapatite(HA) coated dental implants by Ion-Beam Assisted Deposition(IBAD) placed in the surgically created circumferential gap in dogs. Materials and methods: In four mongrel dogs, all mandibular premolars and the first molar were extracted. After an 8 weeks healing period, six submerged type implants were placed and the circumferential cylindrical 2mm coronal defects around the implants were made surgically with customized step drills. Groups were divided into six groups : anodized surface, anodized surface with 150nm HA and heat treatment, anodized surface with 300nm HA and heat treatment, anodized surface with 150nm HA and no heat treatment, and anodized surface with 150nm HA, heat treatment and bone graft, anodized surface with bone graft. The dogs were sacrificed following 12 weeks healing period. Specimens were analyzed histologically and histomorphometrically. Results: During the healing period, healing was uneventful and implants were well maintained. Anodized surface with HA coating and $430^{\circ}C$ heat treatment showed an improved regenerative characteristics. Most of the gaps were filled with newly regenerated bone. The implant surface was covered with bone layer as base for intensive bone formation and remodeling. In case that graft the alloplastic material to the gaps, most of the coronal gaps were filled with newly formed bone and remaining graft particles. The bone-implant contact and bone density parameters showed similar results with the histological findings. The bone graft group presented the best bone-implant contact value which had statistical significance. Conclusion: Within the scope of this study, nano-scale HA coated dental implants appeared to have significant effect on the development of new bone formation. And additional bone graft is an effective method in overcoming the gaps around the implants.

• International Journal of Aeronautical and Space Sciences
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• 제15권3호
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• pp.219-231
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• 2014

We develop a preliminary design optimization procedure in this paper regarding the wing planform in a solar-powered high-altitude long-endurance unmanned aerial vehicle. A high-aspect-ratio wing has been widely adopted in this type of a vehicle, due to both the high lift-to-drag ratio and lightweight design. In the preliminary design, its characteristics need to be addressed correctly, and analyzed in an appropriate manner. In this paper, we use the three-dimensional Euler equation to analyze the wing aerodynamics. We also use an advanced structural modeling approach based on a geometrically exact one-dimensional beam analysis. Regarding the structural integrity of the wing, we determine detailed configuration parameters, specifically the taper ratio and the span length. Next, we conduct a multi-objective optimization scheme based on the response surface method, using the present baseline configuration. We consider the structural integrity as one of the constraints. We reduce the wing weight by approximately 25.3 % from that in the baseline configuration, and also decrease the power required approximately 3.4 %. We confirm that the optimized wing has sufficient flutter margin and improved static longitudinal/directional stability characteristics, as compared to those of the baseline configuration.

• Journal of Electrical Engineering and Technology
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• 제11권3호
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• pp.662-669
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• 2016

In this work, nonlinear control of a three-phase shunt active power filter (SAPF) has been studied and compared to classical control based on proportional integral regulator. The control strategy is based on the direct current method using sliding mode control (SMC), where the aim is to regulate the average voltage across the dc bus of the inverter. Details are given for the control algorithm; the controller is comprised of a current loop which utilizes a hysteresis controller to generate the gating signals for the switching devices, and a nonlinear controller based on SMC law which is different from classical laws based on error between reference and measured output voltage of the inverter. Sliding surface applied in this work contains the whole of state variables, in order to ensure full control of the system behavior in the presence of disturbances that affect the supply source, the load parameters or the reference value. The designed controller offers advantage that it can gives the improvement of dynamic and static performances in cases of large disturbances. A comparison of the effects of PI control and SMC on the APF response in steady stat, under line variations, load variations, and different component variations is performed.

• Journal of Microbiology and Biotechnology
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• 제15권1호
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• pp.66-71
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• 2005

Doxorubicin is a clinically important anticancer polyketide compound that is typically produced by Streptomyces peucetius var. caesius. To improve doxorubicin productivity by S. peucetius, a doxorubicin pathway-specific regulatory gene, dnrI, was cloned into a high-copy-number plasmid containing a catechol promoter system. The S. peucetius containing the recombinant plasmid exhibited approximately 9.5-fold higher doxorubicin productivity compared with the wild-type S. peucetius. The doxorubicin productivity by this recombinant S. peucetius strain was further improved through the optimization of culture media composition. Based on the Fractional Factorial Design (FFD), cornstarch, $K_2HPO_4$, and $MgSO_4$ were identified to be the key factors influencing doxorubicin productivity. The Response Surface Method (RSM) results based on 20 independent culture conditions with varying amounts of key factors predicted the highest theoretical doxorubicin productivity of 11.1 mg/l with corn starch of 46.33 g/l, $K_2HPO_4$ of 4.63 g/l, and $MgSO_4$ of 9.26 g/l. The doxorubicin productivity of the recombinant S. peucetius strain with the RSM-based optimized culture condition was experimentally verified to be 11.46 mg/l, which was approximately 30.8-fold higher productivity compared with the wild-type S. peucetius without culture media optimization.

• International Journal of Air-Conditioning and Refrigeration
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• 제17권2호
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• pp.68-73
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• 2009

In this paper, a numerical study has been carried out to investigate the influence of volute geometries on the performance of a sirocco fan. In order to achieve an optimum volute design and explain the interactions between the different geometric configurations in the volute system, three-dimensional computational fluid dynamics and the 'design of experiment' method have been applied. Several geometric parameters, such as the volute expansion angle, the cut-off position and the bell mouth shape, are employed to improve efficiency and performance. $2^k$ factorial designs were performed to screen the most influential parameters and interactions, and showed that the cut-off position and the bell mouth shape are the most significant parameters. The optimum design was selected as a result of the response surface methodology, and effects of these parameters and their interactions were presented. From the results of computational analyses and experimental data, the performance and efficiency of the sirocco fan were successfully improved. Also, detailed effects of geometric variables of the volute system on the fan performance were discussed.

• 한국추진공학회지
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• 제6권2호
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• pp.65-74
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• 2002

전압력 회복율을 최대로 하기 위한 IRR형 램젯 공기 흡입구 초음속 확산부의 최적형상 설계를 수행하였다. 질량유량을 제약조건으로 고려하고 외부램프에서의 두 번째 경사충격파와 카울립 형상, 그리고 흡입구 목의 단면적을 설계변수로 선택하였다. 효율적인 최적화를 위해 설계변수 변환을 통한 개선된 반응면 기법을 사용하였으며 설계반복을 통해 높은 신뢰도의 반응면을 구성할 수 있었다. 최적화 기법으로 유전자 알고리즘을 사용하였으며, 이차원 Euler Code를 사용하여 공력해석을 수행하였다. 배압조건의 적용을 위해 흡입구 목 뒤로 가상의 노즐을 장착하였고 총 20회의 계산으로 종말충격파 이후의 전압력 회복율이 기준형상에 비하여 14% 향상된 초음속 확산부 최적형상을 설계할 수 있었다.

• Earthquakes and Structures
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• 제12권4호
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• pp.457-468
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• 2017

When studying the vibration of a suspension bridge based on the traffic-bridge coupled system, most researchers ignored the contribution of the pavement response. For example, the pavement was simplified as a rigid base and the deformation of pavement was ignored. However, the action of deck pavement on the vibration of vehicles or bridges should not be neglected. This study is mainly focused on establishing a new methodology fully considering the effects of bridge deck pavement, probabilistic traffic flows, and varied road roughness conditions. The bridge deck pavement was modeled as a boundless Euler-Bernoulli beam supported on the Kelvin model; the typical traffic flows were simulated by the improved Cellular Automaton (CA) traffic flow model; and the traffic-pavement-bridge coupled equations were established by combining the equations of motion of the vehicles, pavement, and bridge using the displacement and interaction force relationship at the contact locations. The numerical studies show that the proposed method can more rationally simulate the effect of the pavement on the vibrations of bridge and vehicles.

• 한국물환경학회지
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• 제28권6호
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• pp.843-850
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• 2012

Due to the fast response to algae bloom issue in drinking water treatment plant, very fast determination methodology for algal toxin is required. In this study, column switching technique based online preconcentration method was combined with high resolution full scan mass spectrometer to save sample preparation time and to obtain fast and accurate result. After parameter optimization of online preconcentration, 1mL filtered sample was directly injected to trap column with switching valve system. Next, target toxins are eluted by 98% acetonitrile and analysed with 150 - 1,100 amu scan range at 50,000 resolving power. Method detection limit (MDL) for microcystin-LR, the most toxic isomer, was 0.1 ng/mL and others such as microcystin-YR, microcystin-RR and nodularin were 0.08, 0.03 and 0.04 ng/mL, respectively. This is the best improved sensitivities with 1mL volume in the literature. Furthermore, due to the use of ultra pressure HPLC (UPLC), the whole method run was completed in 4 min. Real sample applications for 173 sample including 55 surface water and 118 treatment plant samples for raw and treated water could be done within 16 hours. In our calculation, this methodology is roughly 80% faster than the previous manual solid-phase extraction with LC-MS/MS method.

• 한국수소및신에너지학회논문집
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• 제24권1호
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• pp.50-60
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• 2013

High temperature proton exchange membrane fuel cells (PEMFCs) using phosphoric acid (PA) doped polybenzimidazole (PBI) membranes have been concentrated as one of solutions to the limits with traditional low temperature PEMFCs. However, the amount of reported experimental data is not enough to catch the operational characteristics correlated with cell performance and durability. In this study, design of experiments (DOE) based operational optimization method for high temperature PEMFCs has been proposed. Response surface method (RSM) is very useful to effectively analyze target system's characteristics and to optimize operating conditions for a short time. Thus RSM using central composite design (CCD) as one of methodologies for design of experiments (DOE) was adopted. For this work, the statistic models which predict the performance and degradation rate with respect to the operating conditions have been developed. The developed performance and degradation models exhibit a good agreement with experimental data. Compared to the existing arbitrary operation, the expected cell lifetime and average cell performance during whole operation could be improved by optimizing operating conditions. Furthermore, the proposed optimization method could find different new optimal solutions for operating conditions if the target lifetime of the fuel cell system is changed. It is expected that the proposed method is very useful to find optimal operating conditions and enhance performance and durability for many other types of fuel cell systems.