• 대한수의학회지
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• 제48권1호
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• pp.75-81
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• 2008

The rapid evolution of antibiotic resistance in Staphylococcus (S.) aureus is of considerable concern. Methicillin-resistant S. aureus (MRSA) strains are especially one of the greatest public concerns since the treatment of infections is more difficult when encountering resistance. In this study, we conducted a nationwide survey on the antimicrobial resistance of S. aureus isolated from raw meat samples collected from 16 countries, including Korea, and investigated the prevalence of MRSA as a possible source of human infection. Of 1,984 meat samples, S. aureus was isolated from 218 (11.0%) samples consisting of 23 (12.1%) from domestic meat and 195 (10.9%) from imported meat. The isolation rates of poultry meat, pork and beef were 12.8%, 7.0% and 10.0%, respectively. With regard to imported meat, the incidence varied from 4.8% to 16.6% from 13 countries, with the exception of Austria and Poland. In a resistance test to 20 antimicrobial agents, one hundred and eighty-four isolates (84.4%) were resistant to one or more antimicrobial agents tested. Especially, 17 (7.8%), 124 (56.9%) and 28 (12.8%) isolates showed a resistance to 3, 2 and 1 drugs, respectively. One isolate originating from domestic beef was resistant to 7 drugs. Another isolate originating from imported poultry meat showed resistance to oxacillin and methicillin by the disk diffusion test and minimal inhibition concentration methods, but showed negative for detection of the mecA gene.

• Asian-Australasian Journal of Animal Sciences
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• 제14권12호
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• pp.1655-1658
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• 2001

Bovine genome samples were collected from meat of three different beef breeds (Hanwoo, Holstein and imported beef breed) that are commercially merchandized in Korean beef market. Operon B (OPB)-kits were used as random primers (3, 7, 10, 11, 12, 14) in random amplified polymorphic DNA (RAPD) method on whole genome. Each primer provided characteristic bands that were highly polymorphic. Each single primer could provide relatively efficient polymorphic band patterns among breeds. However, use of two or more primers in combination is recommended to improve resolution of experiments with higher molecular weight bands of DNA. In our experiments, OPB-11 resolved well between beef cattle breeds and Holstein. And OPB-7, 12 and 14 could be combined with OPB-11 to identify Hanwoo beef from the other two kinds of beef.

• 한국축산식품학회지
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• 제25권2호
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• pp.203-209
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• 2005

유전자수준의 염기서열에 근거를 둔 유전자 감식기법을 활용하여 개체간의 유전적 차이로 한우육과 젖소육 혹은 수입육의 구별방법을 개발하기 위한 연구가 많이 수행되었다. 그 중 real-time PCR 판별방법은 기존 PCR의 단점인 허위양성을 배제시켜 그 결과의 신뢰성이 높으며, PCR 산물들의 제한효소에 의한 절단 및 겔에서의 확인 등의 과정을 생략시킬 수 있어 짧은 시간 내에 다량의 시료를 분석할 수 있고 민감도가 높아 쇠고기가 포함된 가공식품의 분석도 가능하다는 장점이 있다. 본 연구는 시중에서 한우로 유통되는 쇠고기(생육 또는 양념육)의 DNA를 추출하여 real-time PCR을 통해서 모색유전자의 유전자 형(C-type, C/T-type or T-type)으로 한우육과 젖소육 및 수입육을 구분하였다. 한우육만을 판매한다는 시중 음식점 41개소에서 쇠고기(생육 또는 양념육)를 수거하여 분석한 결과, 분석된 41개의 시료 중 29개가 한우 유전자형으로, 12개의 시료가 젖소 또는 수입육 유전자형으로 판별되었다. 따라서 분석된 시료들의 한우육(T-type) 비율은 $70.1\%$, 그리고 젖소육 또는 수입육 비율은 $29.3\%$(C/T-type; $12.2\%$, C-type; $17.1\%$)이었다.

• 한국축산식품학회지
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• 제24권4호
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• pp.355-360
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• 2004

본 연구는 축우의 모색발현에 관여하는 MC1R, MGF 및 TYRP1 3종류의 모색 유전자의 PCR-RFLP marker를 이용하여 쇠고기 품종 판별기술을 개발하고자 수행하였다. MC1R 유전자의 104번째 아미노산을 지정하는 codon에 GGT 염기를 갖고 있는 Holstein 젖소와 Angus 육우는 제한효소 인지부위가 존재하여 537 bp증폭산물이 절단되어 329와 208bp 두개의 band가 검출되었으나 한우에서는 GTG로 G 염기가 T염기로 치환됨으로써 제한효소 인식부위가 소실되어 537 bp의 단일 bind 만이 검출되었다. 따라서, 이처럼 MC1R 모색유전자의 품종 간 특정 염기서열의 차이가 곧 특정 제한효소의 염기 서열상의 인지 부위 차이를 가져와 한우와 Holstein 젖소 및 Angus 육우 품종간의 RFLP 유전자형 출현에 확실한 차이가 인정되어 한우 품종에 특이적인 MC1R 유전자의 RFLP marker를 이용한 한우육 판별이 가능하였다. 또한, MGF 유전자의 RFLP 유전자형 출현빈도에서 한우는 r/r형이 75％로 출현율이 매우 높은 유전자형으로 분석된 반면 Hereford종은 R/R 형이 80％로 출현율이 매우 높았고 Holstein종과 Angus종은 R/r형이 100％ 출현함으로써 한우와 Holstein 및 수입육우 품종간의 MGF 유전자형 출현빈도에 뚜렷한 차이가 인정되었다. 한편, TYRP1 유전자의 RFLP유전자형을 분석한 결과 모든 품종에서 동일한 RFLP type이 검출되어 TYRP1 모색 유전자를 이용한 쇠고기 품종 구별은 불가능한 것으로 나타났다. 따라서, 소 모색 관련 MC1R과 MGF 두 유전자의 품종 특이적 PCR-RFLP 유전자형은 한우육과 국내산 Holstein젖소고기 및 Angus 수입육간의 품종을 식별하는데 매우 유용한 DNA marker로 이용될 수 있음이 확인되었다.

• 한국기후변화학회지
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• 제2권1호
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• pp.15-32
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• 2011

인간 활동에 의해 증가된 온실가스로 인하여 지구촌 곳곳에서 기상이변이 속출하고 있으며, 세계적으로 온실가스 배출 감축을 위한 노력을 하고 있다. 여기에 발맞추어 우리나라 또한 녹색성장을 기조로 한 저탄소 사회 실현을 위한 노력을 하고 있으며, 이를 위해서는 녹색생활을 통한 저탄소 소비 패턴 정착이 필요하다. 이에 국민들에게 저탄소 생활로의 변화를 통해 저감되는 온실가스 배출량 정보 제공이 요구되는 바, 본 연구에서는 국내에서 소비되는 식품들 중 수입의 비중이 높은 쇠고기와 포도주를 대상으로 하여 국내외산 쇠고기와 포도주의 푸드 마일리지 및 이산화탄소 배출량을 산정하였고, 수입산과 국내산의 이산화탄소 배출량 산정 결과를 이용하여 수입산을 국내산으로 대체할 경우에 저감 가능한 이산화탄소 배출량을 평가하였다. '07년 기준으로 수입 쇠고기 10%를 국내산으로 대체하면 연간 14,000톤 만큼의 이산화탄소 감축이 가능하며, 일 년 중 하루를 수입 쇠고기 대신 국내산으로 대체했을 때에는 연간 384톤 만큼 감축이 가능한 것으로 나타났다. 같은 방법으로 수입 포도주 10%를 국내산으로 대체하면 연간 1,396톤이 감축되고, 일 년 중 하루를 수입 포도주 대신 국내산으로 대체했을 때에는 연간 38톤의 감축이 가능한 것으로 나타났다. 푸드 마일리지와 같은 정량적 지표를 사용하여 국내산 식품의 수요를 유도함으로써 국민들의 실생활 속에서 자연스럽게 저탄소 생활로의 소비 패턴이 이루어질 수 있을 것이며, 앞으로도 이러한 정량적인 이산화탄소 배출량 DB의 개발과 보급이 필요할 것으로 사료된다.

• 한국식품위생안전성학회지
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• 제10권2호
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• pp.89-95
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• 1995

Highly lethal Listeria monocytogenes, causing bromatoxism through vegetables, dairy products, meat products and shellfish etc, was examined for possible contamination in market beef. USDA, FDA, Malthus and Modified Cold Enrichment methods were used for the detection of Listeria spp.. Samples of domestic and imported market beef were collected from local meat shopsat Seoul, Korea. Total two hundreds and six of Listeria spp. were isolated and identified from beef. Among 206 isolates, the number of L. welshimeri was one hundred and twenty-one(44.8%). The numbers of isolated L. innocua, L. murrayi, L. monocytogenes, L. grayi, L. seeligeri, and L. ivanovii were 49(18.1%), 14(5.2%), 12(4.4%), 6(2.2%), 2(0.7%), and 2(0.7%), respectively. Detection rates of Listeria spp. varied among four methods. The highest detection rate of Listeria spp. in market beef was found at USDA method and that of L. monocytogenes was found at Malthus method.

• 대한수의사회지
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• 제16권1112호
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• pp.363-367
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• 1980

An annual incidence of disease and mortality rate was surveyed to the 573 imported beef cattle in sunchun district, Jeonnam during 1979 inorder to evaluate to advanced animal health control and laboratory reutine examinations for piroplasmosis and interna

• 한국식품영양과학회지
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• 제28권3호
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• pp.511-519
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• 1999

To prevent the denaturation of ficin(EC 3.4.22.3) that is a proteolytic enzyme in fig(Ficus carica L.), fig conserve was heated to 55oC. The fig conserve was added as a tenderizer to native Korean cattle beef(KCB), dairy cattle beef(DCB), castrated dairy cattle beef(CDCB), and imported beef(IB). The composition of free amino acids, hydroxyproline content, shear force, cooking loss, morphological changes and sensory evaluation were then investigated to observe the effect of tenderizing beef with fig conserve. Free amino acids and cooking loss of treated beef were higher than those of control, whereas hydroxyproline and shear force were lower. Glutamine in treated beef decreased relatively but asparagine increased. Hydroxyproline was found, in increasing order of abundance, in DCB, CDCB, IB and KCB. By portion, loin was higher than tenderloin in free amino acids, hydroxyproline and shear force but was lower in cooking loss. Observation with a light microgram revealed a surprising loss of muscle fiber in treated beef. In sensory evaluation of uncooked beef, the control was redder than the treated beef(p<0.01~p<0.05). Treatment with fig conserve increased the juiciness of both cooked CDCB and IB(p<0.001) and decreased their hardness(p<0.01~p<0.001).

• 대한수의학회지
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• 제49권4호
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• pp.319-328
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• 2009

This study investigated the epidemiology of Listeria (L.) monocytogenes, a food-borne pathogen. The epidemiology of food-borne pathogens is of great importance for clarifying bacterial origin and preventing bacterial contamination and infection. This work examined 68 L. monocytogenes strains, including 11 reference strains and 57 isolates from imported US beef, domestic meats (beef, pork, chicken meat), raw milk, and milk plants. The random amplified polymorphic DNA (RAPD) techniques were optimized to develop a standard molecular epidemiological analysis of L. monocytogenes. There was great genetic variability among the isolates, which produced 24 and 34 RAPD patterns with primer HLWL85 and HLWL74, respectively. The discriminatory power of the RAPD methods with HLWL85 and HLWL74 primer were very high (DI = 0.957; S ${\geq}$ 80%, S ${\geq}$ 95%). Some RAPD types were specific to origin. A few RAPD types were specific for L. monocytogenes strains belonging to a particular serotype. Using the HLWL85 primer, the strains isolated from milk plants could be distinguished from the other strains. And using the HLWL74 primer, the strains isolated from imported beef (US) could be distinguished completely from the other strains.

• 대한영양사협회학술지
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• 제21권3호
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• pp.203-214
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• 2015

A survey was conducted to determine the perception of youku meat among college students majoring in food and/or nutrition. The survey participants were located nationwide, and the responses from the 2,454 students were analyzed. More male and higher grade students answered that they had heard about youku while only 20.0% had learned about Youku from class. Approximately 37.8% of the subjects recognized youku as 'dairy cattle which are too old to produce milk', 54.0% as 'all cattle grown for the purpose of meat', and 23.1% as 'all cattle except for Hanwoo'. Only 37.4% recognized youku correctly. Compared with the same quality grade, 25.3% recognized youku meat as being cheaper than imported beef, and only 25.6% of them recognized that youku meat has less fat than imported beef. As much as 83.3% of subjects did not know whether or not they were served youku meat, and 23.7% of subjects wanted increased availability of youku meat. As much as 22.9% of subjects opposed the increased use of youku meat, and the reasons were "it does not taste good" (18.1%), "it is not Hanwoo" (15.1%), "it is not sanitary" (13.1%), and "it is imported" (6.0%). The findings provide basic information on barriers regarding youku meat promotion among subjects who will be dieticians in food service or managers in purchase departments of catering companies in the future.