• KSTLE International Journal
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• 제4권1호
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• pp.31-36
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• 2003

Sliding and impact/sliding wear test in room temperature air and water were performed to evaluate the effect of spring shapes on the wear mechanism of a fuel rod. The main focus was to quantitatively compare the wear behavior of a fuel rod with different support springs (i.e. two concaves, a convex and a flat shape) using a ratio of wear volume to worn area (De)-The results indicated that the wear volumes at each spring condition were varied with the change of test environment and loading type. However, the relationship between the wear volume and worn area was determined by only spring shape even though the wear tests were carried out at different test conditions. From the above results, the optimized spring shape which has more wear-resistant could be determined using the analysis results of the relation between the variation of De and worn surface observations in each test condition.

• 환경영향평가
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• 제22권2호
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• pp.125-134
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• 2013

Plant virus coat (CP) gene-mediated protection is one of the best known approaches to protect against virus resistant transgenic plants. Transgenic N. benthamiana plants containing the CP gene of Zucchini green mottle mosaic virus (ZGMMV) were used for the environmental risk assessment of the living modified (LM) plants with plant virus resistance. The most optimal co-infection method of both ZGMMV and CMV (Cucumber mosaic virus) on Non-LM and CP-expressing LM tobacco plants was established and co-infection of CMV and ZGMMV was confirmed by polymerase chain reaction (PCR). To address the effects of LM tobacco plants on the mutation of the virus, in-vitro transcripts of CP and Replicase (Rep) derived from CMV and/or ZGMMV were inoculated onto Non-LM or LM tobacco plants. Mutation frequency of CP and Rep from CMV and ZGMMV was examined through six serial passages in Non-LM and LM tobacco plants. Little actual frequency of mutation was estimated, probably due to the limited number of transgenic plants tested in this study. However, it does not suggest environmental safety of these CP-mediated LM plants. Further study at a larger scale is needed to evaluate the environmental risk associated with the CP-expressing LM plants.

• 한국윤활학회:학술대회논문집
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• 한국윤활학회 2004년도 학술대회지
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• pp.37-40
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• 2004

The impact/sliding wear tests have been performed in high temperature high pressure water in order to evaluate the effect of spring shape on the wear behavior of a spring supported tube for nuclear fuel fretting study. The results indicate that the tube wear volume and the size of the wear scar are closely related to each spring shape. From the analysis of the wear scar, it is possible to extract the real worn area (Aw) from the size of the wear scar (At). In addition, we found that the wear volume has a linear relation with the real worm area rather than the size of wear scar and this was only determined by each spring shape in the high temperature and pressure water condition. From the above results, it is possible to evaluate the wear resistant spring using the correlation between the variation of the real worn area and the wear behavior at each spring.

• Toxicological Research
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• 제26권4호
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• pp.301-313
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• 2010

Growth promoters including hormonal substances and antibiotics are used legally and illegally in food producing animals for the growth promotion of livestock animals. Hormonal substances still under debate in terms of their human health impacts are estradiol-$17\beta$, progesterone, testosterone, zeranol, trenbolone, and melengestrol acetate (MGA). Many of the risk assessment results of natural steroid hormones have presented negligible impacts when they are used under good veterinary practices. For synthetic hormonelike substances, ADIs and MRLs have been established for food safety along with the approval of animal treatment. Small amounts of antibiotics added to feedstuff present growth promotion effects via the prevention of infectious diseases at doses lower than therapeutic dose. The induction of antimicrobial resistant bacteria and the disruption of normal human intestinal flora are major concerns in terms of human health impact. Regulatory guidance such as ADIs and MRLs fully reflect the impact on human gastrointestinal microflora. However, before deciding on any risk management options, risk assessments of antimicrobial resistance require large-scale evidence regarding the relationship between antimicrobial use in food-producing animals and the occurrence of antimicrobial resistance in human pathogens. In this article, the risk profiles of hormonal and antibacterial growth promoters are provided based on recent toxicity and human exposure information, and recommendations for risk management to prevent human health impacts by the use of growth promoters are also presented.

• Steel and Composite Structures
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• 제50권6호
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• pp.689-703
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• 2024

Construction of high-rise structures, formwork systems that can be installed quickly, resistant to external loads, can be used more than once, have become a necessity. Timber and composite timber materials are preferred in the formation of such formwork systems due to their durability, ease of assembly, light weight and easy to use more than one time. Formwork beams are the most commonly used structural component in the formation of such formwork systems, and these beams can be damaged for different reasons during their lifetime. In this study, H20 top P type timber formwork beams with 1800 and 2450 mm length which is among the products of DOKA(c) company is damaged under the effect of static loading up to a high load level of 85% of the maximum ultimate capacity and after being retrofitted using anchored CFRP strips, performance and behavior of the beams under the influence of various loading types such as static, fatigue and impact are investigated experimentally. Two different lengths of retrofitted timber formwork beams were tested by applying monotonic static, fatigue and impact loading and comments were made about the effects of the retrofit method on performance under different loading types.

• 복합신소재구조학회 논문집
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• 제1권3호
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• pp.41-48
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• 2010

본 논문에서는 폭발하중으로 발생하는 폭풍파의 충격하중과 폭풍파로 초래된 파편의 충돌하중을 동시에 받는 철근 콘크리트 벽체 구조물의 비선형 충돌 손상거동 해석이 수행된다. 이를 위해 먼저 가상 폭발사고 시나리오로부터 철근 콘크리트 구조물에 충격과 충돌 하중이 복합적으로 작용하는 경우를 선정한다. 폭발하중으로 인한 구조물의 저항성능을 확보하기 방안으로는 복합신소재를 보강하는 경우가 고려되고, 복합신소재를 보강하지 않은 철근 콘크리트 벽체 구조물과 보강성능을 비교하여 제시한다. 또한, 막대한 시설과 비용 투자가 요구되는 폭발실험과 근접한 해석을 도출하기 위하여 실제 충격과 충돌 현상을 정확하게 묘사한 구성방정식과 상태방정식을 포함시킨 정교한 해석을 수행한다. 폭발하중과 같은 순간적인 동적 문제를 해석하기 위하여 외연적 고속충돌 해석 프로그램인 AUTODYN-3D을 활용하여 두 가지 대상구조물에 대한 수치 모의실험을 수행하고 복합신소재로 보강된 철근 콘크리트 벽체 구조물의 보강성능을 입증한다.

• Advances in nano research
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• 제12권5호
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• pp.529-547
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• 2022

Graphene is one of the strongest, stiffest, and lightest nanoscale materials known to date, making it a potentially viable and attractive candidate for developing lightweight structural composites to prevent high-velocity ballistic impact, as commonly encountered in defense and space sectors. In-plane twist in bilayer graphene has recently revealed unprecedented electronic properties like superconductivity, which has now started attracting the attention for other multi-physical properties of such twisted structures. For example, the latest studies show that twisting can enhance the strength and stiffness of graphene by many folds, which in turn creates a strong rationale for their prospective exploitation in high-velocity impact. The present article investigates the ballistic performance of twisted bilayer graphene (tBLG) nanostructures. We have employed molecular dynamics (MD) simulations, augmented further by coupling gaussian process-based machine learning, for the nanoscale characterization of various tBLG structures with varying relative rotation angle (RRA). Spherical diamond impactors (with a diameter of 25Å) are enforced with high initial velocity (V_i) in the range of 1 km/s to 6.5 km/s to observe the ballistic performance of tBLG nanostructures. The specific penetration energy (E_p^*) of the impacted nanostructures and residual velocity (V_r) of the impactor are considered as the quantities of interest, wherein the effect of stochastic system parameters is computationally captured based on an efficient Gaussian process regression (GPR) based Monte Carlo simulation approach. A data-driven sensitivity analysis is carried out to quantify the relative importance of different critical system parameters. As an integral part of this study, we have deterministically investigated the resonant behaviour of graphene nanostructures, wherein the high-velocity impact is used as the initial actuation mechanism. The comprehensive dynamic investigation of bilayer graphene under the ballistic impact, as presented in this paper including the effect of twisting and random disorder for their prospective exploitation, would lead to the development of improved impact-resistant lightweight materials.

• 열처리공학회지
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• 제13권1호
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• pp.27-34
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• 2000

This present study were investigated effect of Ni contents on the microstructure and strength characteristic in 9Cr ferritic heat-resistant steel added 1.7%W in place of Mo in order to restrain laves phase formation. The result obtained from this study are as follow. Volume fraction, number of particles per unite area and particle size of carbide decreased with increase of Ni contents. Other side, carbides of $M_{23}C_6$ type was mainly precipitated in this steel, but laves phases could not precipitated in spite of increasing of aging time. With increase of tempering temperature, hardness was increased, and maximum value was showed around 873k by secondary hardening due to precipitation of $W_2C$ type carbide and then, was decreased. Tensile and yield strength due to decrease precipitation amount of carbide and number of particles per unite area was decreased, but elongation and impact value was increased. In case of aged specimen after tempering than tempered specimen, strength was higher and elongation was lower due to increasing of precipitated amount of carbide and number of particles per unite area.

• IMMUNE NETWORK
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• 제21권2호
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• pp.13.1-13.19
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• 2021

Macrophages are important for the first line of defense against microbial pathogens. Integrin CD11b, which is encoded by Itgam, is expressed on the surface of macrophages and has been implicated in adhesion, migration, and cell-mediated cytotoxicity. However, the functional impact of CD11b on the inflammatory responses of macrophages upon microbial infection remains unclear. Here, we show that CD11b deficiency resulted in increased susceptibility to sepsis induced by methicillin-resistant Staphylococcus aureus (MRSA) infection by enhancing the pro-inflammatory activities of macrophages. Upon infection with MRSA, the mortality of Itgam knockout mice was significantly higher than that of control mice, which is associated with increased production of TNF-α and IL-6. In response to MRSA, both bone marrow-derived macrophages and peritoneal macrophages lacking CD11b produced elevated amounts of pro-inflammatory cytokines and nitric oxide. Moreover, CD11b deficiency upregulated IL-4-induced expression of anti-inflammatory mediators such as IL-10 and arginase-1, and an immunomodulatory function of macrophages to restrain T cell activation. Biochemical and confocal microscopy data revealed that CD11b deficiency augmented the activation of NF-κB signaling and phosphorylation of Akt, which promotes the functional activation of macrophages with pro-inflammatory and immunoregulatory phenotypes, respectively. Overall, our experimental evidence suggests that CD11b is a critical modulator of macrophages in response to microbial infection.

• Asian Pacific Journal of Cancer Prevention
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• 제14권12호
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• pp.7529-7536
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• 2013

Background and Aims: MicroRNA-21 (miR-21) is reported to be overexpressed and to contribute to proliferation, apoptosis and gemcitabine resistance in pancreatic ductal adenocarcinomas (PDACs). The aims of this study were to explore regulation of miR-21 expression by epigenetic change and its impact on chemoresistance and malignant properties of of pancreatic cancer. Materials and methods: We retrospectively collected 41 cases of advanced pancreatic cancer patients who were sensitive or resistant to gemcitabine and assessed levels of serum circulating miR-21 for correlation with cytotoxic activity. Histone acetylation in the miR-21 promoter was also studied in gemcitabine-sensitive and gemcitabine-resistant PDAC cells. Gemcitabine-resistant HPAC and PANC-1 cells were transfected with pre-miR-21 precursors (pre-miR-21) and antisense oligonucleotides (anti-miR-21), and were treated with TSA. Finally, invasion and metastasis assays were performed and alteration in mir-21, PTEN, AKT and pAKT level was evaluated in these cells. Results: Serum miR-21 levels were increased in gemcitabine-resistant PDAC patients compared with gemcitabine-sensitive subjects. The miR-21 levels were increased in 6 PDAC cells treated with gemcitabine significantly, associated with 50% inhibitory concentrations ($IC_{50}s$). Histone acetylation levels at miR-21 promoter were increased in PDAC cells after treatment with gemcitabine. Enhanced invasion and metastasis, increased miR-21 expression, decreased PTEN, elevated pAKT level were demonstrated in gemcitabine-resistant HPAC and PANC-1 cells. Pre-miR-21 transfection or TSA treatment further increased invasion and metastasis ability, decreased PTEN, and elevated pAKT levels in these two lines. In contrast, anti-miR-21 transfection could reverse invasion and metastasis, and PTEN and pAKT expressions induced by gemcitabine. Conclusions: MiR-21 upregulation induced by histone acetylation in the promoter zone is associated with chemoresistance to gemcitabine and enhanced malignant potential in pancreatic cancer cells.