• 약학회지
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• 제49권1호
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• pp.6-10
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• 2005

Immunoglobulin Y (IgY) obtained from chicken as the immunization host brings several advantages to antibody production, such as improved yield, lower cost, longer stability and higher specificity than mammalian immunoglobulin. In the present study, we attempted to produce IgY against a sialic acid-binding lectin, Maackia fauriei agglutinin (MFA), from egg yolk of white Leghorn hens. For the isolation of IgY from egg yolk, we applied a water dilution method. The weekly yield of IgY was determined by enzyme-linked immunosorbent assay, with a final yield of anti-MFA IgY from total IgY of approximately $1\%$. The yielded IgY were used to prepare IgY-affinity column conjugated with CNBr-activated Sepharose 4B, which resulted in the lectin being successfully purified in a single step from Maackia fauriei. This purified lectin exhibited the same hemagglutination activity as lectin purified using conventional purification methods.

• KSBB Journal
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• 제14권6호
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• pp.677-681
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• 1999

계란의 난황으로부터 유래한 IgY의 분리와 정제과정이 흐름을 정리한 결과, 난황에서의 수용성 단백질의 분리단계가 단백질의 수율을 증가시키는데 가장 큰 영향을 미치는 것으로 판단되었다. 본 연구에서는 자연 침전방법을 통하여 실험하였는데 수율의 증가를 위해서는 원심분리와 같이 수용성 단백질의 양을 많이 얻을 수 있는 방법이 필요하고, 인지질이나 lipoprotein등의 성분의 제거효율을 높이기 위해서는 x-carrageenan등과 같은 natural gum성분의 첨가와 같은 방법이 필요하다. 그러므로 자연침전을 이용하여 수율의 증가측면과 비용의 절감의 효과를 얻을 수 있었다. 보다 효율적인 IgY의 분리를 위해서는 초기 단계에서 수율의 극대화와 불순성분 제거의 최대화가 동시에 요구되고 있다. 전처리 후의 시료를 이용하여 이온교환 크로마토그래피와 gel filtration chromatography를 통해 순도 90% 이상의 IgY를 얻을 수 있었다.

• Journal of Periodontal and Implant Science
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• 제29권2호
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• pp.349-355
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• 1999

The present study has been performed to develop a PCR technology to identify human immunoglobulin(Ig) allotypes with restriction fragment length polymorphism(RFLP) using a probe. Genomic DNA were ampilified with PCR tecnology using primers from peripheral blood lymphocytes of 10 periodontal patiens, whose Ig allotypes have been pre-determined by serological tecnique using heagglutination technique. The result indicated that the RFLP patterns could successfully differentiate the Ig allotypes, which suggests that this technology can be developed as a tool useful for population genetics studies.

• 대한영상의학회지
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• 제84권6호
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• pp.1373-1377
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• 2023

면역글로불린 G4 (immunoglobulin G4; 이하 IgG4) 관련 폐 질환은 다양한 임상 경과를 가질 수 있다. 우리가 아는 한, 호전과 악화를 반복하는 폐 침윤을 동반한 IgG4 관련 폐 질환의 단독 보고는 매우 드물다. 36세 여성이 수술 전 평가에서 폐에 결절성 및 둥근 모양의 간유리음영을 발견했다. 폐 병변은 후속 흉부 컴퓨터 단층 촬영에서 호전과 악화를 반복하였다. 병변은 수술 후 IgG4 관련 폐 질환으로 확인되었다.

• 대한영상의학회지
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• 제82권3호
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• pp.575-588
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• 2021

면역글로불린 G4 (immunoglobulin G4; 이하 IgG4) 관련 질환은 특징적인 병리 소견을 동반한 섬유화를 일으키는 전신 염증성 질환이다. 영상검사는 IgG4 관련 질환의 진단 및 치료 반응 평가에 필수적이다. 본 임상화보는 IgG4 관련 질환의 다양한 임상 증례를 통해 영상 소견 및 감별진단을 제시함으로써 IgG4 관련 질환에 대한 이해의 폭을 넓히고자 한다.

• Journal of Periodontal and Implant Science
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• 제44권6호
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• pp.274-279
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• 2014

Purpose: To evaluate whether the levels of immunoglobulin G (IgG) antibody to Tanerella forsythia are associated with periodontal status. Methods: Patients with a diagnosis of chronic periodontitis were considered candidates for the study; thus 80 chronic periodontitis patients and 28 healthy persons (control group) were invited to participate in this investigation. The presence of T. forsythia was detected by polymerase chain reaction (PCR) analysis using primers designed to target the respective 16S rRNA gene sequences. Peripheral blood was collected from each subject to identify the IgG1 and IgG2 serum antibodies against T. forsythia. All microbiological and immunological laboratory processes were completed blindly, without awareness of the clinical status of the study patients or of the periodontal sites tested. Results: The bivariate analysis showed that lower mean levels of clinical attachment level (CAL) and probing depth were found in the presence of the IgG1 antibody titers against whole-cell T. forsythia; however, only the difference in CAL was statistically significant. In the presence of the IgG2 antibody titers against whole-cell T. forsythia, the periodontal parameters evaluated were higher but they did not show statistical differences, except for plaque. The unadjusted linear regression model showed that the IgG1 antibody against whole-cell T. forsythia in periodontitis patients was associated with a lower mean CAL (${\beta}=-0.654$; 95% confidence interval [CI], -1.27 to -0.28; P<0.05). This statistically significant association remained after adjusting for possible confounders (${\beta}=-0.655$; 95% CI, -1.28 to -0.29; P<0.05). On the other hand, smoking was a statistically significant risk factor in the model (${\beta}=0.704$; 95% CI, 0.24 to 1.38; P<0.05). Conclusions: Significantly lower mean levels of CAL were shown in the presence of the IgG1 antibody titers against whole-cell T. forsythia in periodontitis patients. Thus, the results of this study suggest that IgG1 antibody to T. forsythia may have been a protective factor from periodontitis in this sample.

• 대한영상의학회지
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• 제83권5호
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• pp.1168-1174
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• 2022

면역글로불린 G4 (Immunoglobulin G4; 이하 IgG4) 연관 질환은 혈청 IgG4의 상승과 조직으로의 IgG4 양성 형질세포의 침윤을 특징으로 하는 드문 섬유 염증성 질환이다. 흉부 전산화단층촬영에서 IgG4 연관 폐 질환은 다양한 영상의학적 소견을 보여 다른 질환과의 감별이 어렵고 특히 고형 결절 혹은 종괴의 형태로 나타나는 경우 폐암과의 감별이 필요하다. IgG4 연관 폐 질환에서 나타날 수 있는 다른 영상의학적 소견 또한 폐암과 연관되어 나타나는 전이나 림프관성 암종증처럼 보일 수 있다. 저자들은 수술을 통하여 병리학적으로 확진된 IgG4 연관 폐 질환 2예를 경험하였다. 두 증례는 CT에서 고형결절 혹은 종괴의 형태로 나타났으며 폐암과 연관되어 보일 수 있는 다른 영상의학적 소견을 동반하고 있어 폐암과의 감별이 매우 어려웠다. 혈청 IgG4 수치나 다른 장기의 침범 여부가 감별에 도움을 줄 수 있으나 정확한 확진을 위해서 조직검사가 필요하다.

• IMMUNE NETWORK
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• 제8권2호
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• pp.59-65
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• 2008

Background: Toll-like receptors (TLRs) detect microbial infection and can directly induce innate host defense responses, which are thought to play critical roles in protecting the tubotympanum from infection. However, little is known about the relationship between TLRs, which are related to innate immunity, and immunoglobulins, which are related to adaptive immunity, in recurrent otitis media with effusion (OME). We therefore investigated the expression of TLR2 and TLR4 and immunoglobulin in children with OME. Methods: The study population consisted of 72 children with OME, 31 with more than 4 episodes in 12 months or more than 3 episodes in 6 months (otitis-prone group), and 41 with fewer than 3 episodes in 12 months (non-otitis prone group). The expression in middle ear effusion of TLR2 and TLR4 mRNA, as determined by Real time- -polymerase chain reaction (RT-PCR), and the concentrations of IgG, IgA, and IgM, as determined by Enzyme-linked immunosorbent assay(ELISA), were compared between the two groups. Results: Expression of TLR2 and TLR4 mRNA was lower in the otitis prone than in the non-otitis prone group, but the difference was not statistically significant (p>0.05). Between group differences in the concentrations of IgG, IgA and IgM in effusion fluid were not significant (p>0.05), and there were no correlations between immunoglobulin concentration and the expression of TLR2 and TLR4. Conclusion: Although there was a trend toward lower expression of TLR2 and TLR4 in the otitis-prone group, the differences, and those in immunoglobulin concentration, did not differ significantly between the otitis-prone and non-prone groups.

• 대한영상의학회지
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• 제84권3호
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• pp.719-725
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• 2023

면역글로불린 G4(immunoglobulin G4; 이하 IgG4) 관련 질환은 드문 전신성 섬유염증 상태로 IgG4 형질 세포가 풍부한 림프구 침윤과 관련된 기관 비대 또는 종창성 병변을 특징으로 한다. 우리는 48세 여자 환자에서 드문 소견인 연조직 종괴로 발현된 왼팔의 피하층을 침범한 IgG4 관련 질환 증례를 보고하고자 한다. 초음파 및 자기공명영상을 촬영하였으며 악성 또는 염증 병변이 의심되는 불규칙하며 침윤성 경계를 갖는 연조직 종괴가 관찰되었다. 우리는 IgG4 관련 질환의 진단 기준, 조직 병리학적 특징, 영상의학적 특징, 및 치료 방법에 대해 논의하고자 한다.

• Animal Bioscience
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• 제36권8호
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• pp.1241-1251
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• 2023

Objective: Egg yolk immunoglobulin (IgY) is an antibiotic alternative to prevent and fight intestinal pathogenic infections. This study aimed to investigate the effects of sodium alginate/chitosan/sodium alginate IgY microcapsules on the growth performance, serum parameters, and intestinal health of broiler chickens. Methods: One-day-old broilers (Ross 308) were divided into five treatments, each with 10 replicates of five chickens. The dietary treatments were maintained for 28 days and consisted of a basal diet (NC), basal diet + 500 mg chlortetracycline/kg diet (CH), basal diet + 50 mg non-microencapsulated IgY/kg diet (NM), basal diet + 600 mg low levels microencapsulated IgY/kg diet (LM), and basal diet + 700 mg high levels microencapsulated IgY/kg diet (HM). Results: Throughout the 28-day trial period, the NM, LM, HM, and CH groups increased average daily gain compared with the NC group (p<0.05), and the HM group reduced feed conversion ratio compared with the CH group (p<0.05). The LM and HM groups increased relative organ weights of thymus and spleen compared with the CH and NM groups (p<0.05). The HM group improved the duodenal, jejunal and ileum villi height (VH) and villus height to crypt depth ratio (VH:CD) compared with the CH and NM groups (p<0.05). Compared with the CH group, the HM group increased serum immunoglobulin (IgA), immunoglobulin G (IgG), superoxide dismutase, total antioxidant capacity, and glutathione peroxidase levels (p<0.05), and decreased serum malondialdehyde levels (p<0.05). Compared with the NC group, the NM, LM, HM, and CH groups reduced colonic Escherichia coli and Salmonella levels (p<0.05). and the HM group promoted the levels of lactic acid bacteria and bifidobacteria compared with the CH group (p<0.05). Conclusion: Microencapsulation could be considered as a way to improve the efficiency of IgY. The 700 mg high levels microencapsulated IgY/kg diet could potentially be used as an alternative to antibiotics to improve the immune performance and intestinal health, leading to better performance of broiler chickens.