• 한국동물학회지
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• 제33권2호
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• pp.152-157
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• 1990

면역조직화학적 방법을 이용하여 생쥐 난소에서 $\beta$-endorphin이 생성되는 부위를 조사하기 위하여 본 실험을 행하였다. 성숙한 생쥐를 4% neutral buffered paraformaldehyde로 관류고정하였으며 난소를 적출한 후 cryostat로 절편을 만들어 avidin-biotin complex(ABC)를 이용하여 면역색을 하였다. $\beta$-endorphin에 대한 항체반응은 주로 황체에서 일어났으며, theac interna와 theca externa에서는 반응이 나타나지 않았다. 황체에서의 염색 양상은 퇴화가 많이 진행된 황체세포에서 보다 강한 연색반응을 관찰할 수 있었다. 때로 황체세포에서 유래된 것으로 보이는 간질세포에서도 양성반응을 관찰할 수 있었다. 이외에도 large antral follicle의 여포강에 인접한 과립세포에서 약한 반응을 보여주었으나, 제 1차 여포에서는 양성반응이 나타나지 않았다.

• 한국동물학회지
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• 제37권4호
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• pp.478-487
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• 1994

The developmental changes of three enteroendocrine cells, i.e. gastrln, somatostatin and serotonin, of gastric and small intestinal mucosa in pre- and postnatal rat were examined by peroxidase-antiperoxidase (PAP) method. In the course of development, gastrin cells were obsenred in the pyloric gland region and the whole part of small intestine, while somstostatin and serotonin cells in the whole gastric gland region and small intestine. More entroendocrine cells were detected in the pyloric gland region and duodenum than in the other portion. In the stomach, gastrin, somatostatin and serotonin ceils were first obsenred in the pyloric Bland region on 17, 19 and 19 days of gestation respectively. The small intestinal gastrin and serotonin cells were first appeared in the duodenum and iriunum on 17 and 15 days of gestation respectively, and somatostBtin cells in duodenum on 17 days of gestation. The number of cells examined from the stomach were increased from fetal to weanling period and showed a decrease during adult period: the notable increase was shown at the end of suckling period or at early weanling period. The cells of the small intestine increased from fetal to suckling period, especially, these cells markedly increased at the end of fetal period or at early suckling period, and decreased from weanling period. The shape of these cells was oval or fusiform during fetal period. In the stomach, most of gastrin cells turned out to be oval and open-type from suckling period, while the remaining two tripes of cells were oval and open- or closed-type. In the small intestine, 311%Ves of cells examined were changed to fusiform and open-type from the end of fetal period. Three types of cell were distributed over the stratified epithelium on 15 and 17 days of gestation. In the stomach, these cells were distributed lower gastric pit and gland from the following fetal period, and were detected mainly on the upper part of gland from suckling period, and then obsenred on the whole part of gland. In the small intestine, most of cells distributed over only between epithelium of villi on 19 days of gestation, increased in number on the crypt from following fetal period, and also observed abundantly in the crypt at adult period.