• Toxicological Research
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• 제7권1호
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• pp.29-35
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• 1991

Barbiturates are commonly abused tranquilizer and a rapid method to determine these drugs in biological samples is needed. In this study, was screened barbiturates in urine specimens by the fluorescence polarization immunoassay method(FPIA) and the positive samples were confirmed and identified by the more definitive GC/MS method. Fifteen positive smples which have barbiturate values higher than 0.5 ng/ml were analyzed by the GC/MS method. Eight samples were identified as phenobarbital and five samples were identified as crotilbarbitone.

• Bulletin of the Korean Chemical Society
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• 제27권4호
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• pp.479-483
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• 2006

We developed a microfluidic immunoassay platform for the detection of various analytes such as bacterial pathogen by packing antibody-immobilized glass beads in spatially-isolated microchambers on a microfluidic device. Primary amines of antibody were covalently conjugated to carboxyl-terminated glass beads previously treated with aminosilane followed by glutaraldehyde. Through this covalent binding, up to 905 $\mu$g immunoglobulin G (IgG) per gram of glass beads was immobilized. For application, glass beads attaching antibody specific to Escherichia coli O157:H7, a foodborne pathogen, were packed into a microfluidic device and used for the detection of the serotype. This prototype immunoassay device can be used for the simultaneous detection of multiple analytes by sequentially packing different-sized glass beads attaching different antibody in discrete microchambers on a single microfluidic device.

• Bulletin of the Korean Chemical Society
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• 제34권2호
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• pp.595-599
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• 2013

The coordination of pyridyl-N to pentacyanoferrate for the detection of small organic antigens in solution is presented. The unique contribution of this paper is the direct conjugation of pyridyl-N in small organic antigens to pentacyanoferrate. Pentacyanoferrate is promising as an electrochemical label owing to its good electro-chemical properties, which can be utilized to generate an electrical signal in homogeneous electrochemical immunoassays. The facilely synthesized pyridyl-N to pentacyanoferrate was characterized by the electrochemical and spectroscopic methods. Hippuric acid (HA) has been detected competitively on the interaction of free HA and pentacyanoferrate-(4-aminomethylpyridine-hippuric acid) (Fe-HA) to its antibody, with the detection limit of 0.50 ${\mu}g\;mL^{-1}$. While pentacyanoferrate-based immunoassay is in its simplicity and infancy, the proposed immunoassay offers attractive opportunities for developing pyridyl-N-based the electrochemical detection of small organic antigens in the health care area.

• 전기화학회지
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• 제17권3호
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• pp.172-178
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• 2014

이 논문에서는 체내에 존재하는 작은 유기물의 하나인 마뇨산을 빠르고 정량적으로 검출하기 위하여 전기화학적 면역분석법을 이용하였다. 마뇨산은 톨루엔이 인체에 노출되었을 때의 주된 신진 대사 물질로서 대표적인 생화학적 지표물질이다. 톨루엔 노출에 대한 신속하고 정확한 관찰은 산업현장에서 건강관리를 위하여 매우 중요하다. 그래서 마뇨산을 빠르고 정확하게 검출하기 위하여 전기화학적 면역분석법을 실시하였다. 본 연구에서 제시하는 새로운 면역분석방법은 전기화학적 활성물질의 하나인 도파민을 측정물질인 마뇨산과 직접 공유결합을 통하여 항원이 포함된 복합체를 합성하였다. 전기화학적 측면에서 도파민은 두 개의 하이드록시기를 가지고 있어 매우 뛰어난 산화/환원 신호를 보인다. 또한 도파민-마뇨산 복합물도 뛰어난 산화/환원 신호를 보이기 때문에 면역 분석에 적합하다. 도파민-마뇨산 복합물과 마뇨산의 항체와의 균질경쟁반응을 전기화학적인 방법을 통하여 관찰하였다. 본 면역분석을 통하여 실시한 결과는 마뇨산의 농도가 0.010~2.500 mg/mL 까지 정량적으로 분석됨으로써 실제 면역 센서에 적용할 수 있음을 확인하였다.

• 공업화학
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• 제30권4호
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• pp.429-434
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• 2019

본 논문에서는 수용성의 CdSe/ZnS 양자점을 합성하고 이에 항체기능성을 도입하여 lateral flow immunoassay (LFIA) 플랫폼에 융합하여 폐암 질병진단에 활용 가능한 단백질 바이오마커[예: 인간 혈청 아밀로이드 A-1 (hSAA1)]의 농도 분석에 적용하고자 한다. 면역분석법 센서 스트립은 니트로셀룰로오즈 막에 테스트라인과 대조라인으로 각각 항hSAA1 단일클론항체(10G1)(anti-hSAA1)와 항chicken IgY (anti-chicken IgY)를 스프레이하여 제작하였다. 이와 함께, 유기상에서 합성된 CdSe/ZnS 양자점은 카르복실기로 변형된 알케인티올기를 이용한 리간드 교환방법으로 수용성으로 전환하였으며, 이에 타겟 단백질인 hSAA1에 특이적으로 결합 가능한 항체인 항hSAA1 단일클론항체(14F8)로 컨쥬게이션하여 형광검출용 입자[QDs-anti hSAA1 (14F8)]로 사용하였다. 제작된 LFIA 스트립 위에 순차적으로 다른 농도의 hSAA1과 QDs-anti hSAA1 (14F8)의 복합체를 흘려주면, 테스트라인에 anti hSAA1 (10G1)/hSAA1/QDs-anti hSAA1 (14F8) 샌드위치 복합체가 형성되어 양자점에 의한 발광신호가 검출됨을 측정하였다. 최적화된 측방흐름이 가능한 완충용액 조건에서 100 nM 농도의 hSAA1 단백질의 유무를 5 min 안에 눈으로 확인 가능하였다.

• 한국전기화학회:학술대회논문집
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• 한국전기화학회 2005년도 춘계총회 및 학술발표회
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• pp.30-30
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• 2005

• 대한세포병리학회지
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• 제12권1호
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• pp.25-30
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• 2001

Tuberculous lymphadenitis is not uncommon in Korea. Therefore, an inexpensive, safe and rapid method is needed to diagnose the tuberculous lymphadenitis. Flne needle aspiration cytology Is a good method for this purpose, but has several limitations in the diagnosis of tuberculous lymphadenitis, especially when the presence of acid-fast bacilli is not proved. To evaluation the usefulness of the polymerase chain reaction with enzyme immunoassay technique in the detection of Mycobacterium tuberculosis (M. tuberculosis) In the cervical Iymph node asplrates, the authors performed fine needle aspiration cytology and M. tuberculosis PCR with enzyme immunoassay for mycobacterial DNA sequences from 15 cases of the fine needle aspirates. Cytomorphologically, the cases were categorized into three types: predominantly necrotic materials; typical epithelioid cell granulomas with or without slant cells and caseous necrosis; and non-tuberculous lesions, such as reactive lymphadenitis, abscess, metastatic carcinoma and malignant lymphoma. M. tuberculosis DNA was found in 8 of 15 cases by PCR with enzyme immunoassay. Negative findings on PCR were achieved in 7 cases, which revealed non-tuberculous tymphadenopathy. In conclusion, we suggest that M. tuberculosis PCR with enzyme immunoassay using the fine needle aspirates is a very useful tool for the diagnosis of tuberculous lymphadenitis.

• 대한수의학회지
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• 제34권3호
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• pp.529-536
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• 1994

To establish more specific and simple diagnostic methods for detection of the antibodies and antigens of Aujeszky's disease virus(ADV), we designed indirect dot-immunoassay(IDI) and double sandwich dotimmunoassay(DSDI) using the solid phases of nitrocellolose paper and polystyrene plate. The diagnostic efficacy of these methods was investigated. As the sensitivity of IDI was tested by various virus concentration, the specimens with the virus titer above $10^{4.0}TCID_{50}/0.2ml$ showed positive reaction, but that below $10^{1.0}TCID_{50}/ml$ revealed negative. Tonsil emulsion at the virus titer of $10^{4.5}TCID_{50}/0.2ml$ showed the highest sensitivity as diluted by 1/100. In detection of ADV antigens from the various tissues of the rats and pigs infected with ADV, IDI using monoclonal antibody showed the higher specificity as compared with IDI using polyclonal antibody and virus isolation method. The efficacy of the DSDI for detection of ADV antibody was compared with other tests. The sensitivity of DSDI was higher than virus neutralization(VN) and agar gel immunodiffusion test(AGID). Meanwhile, specificity of DSDI was lower than AGID, but similar to IDEA. In comparison with VN test, DSDI showed 96.9% agreement to VN test that is the highest of three tests. In general, application of polyclonal antibody in both tests caused the higher sensitivity but the lower specificty.

• 대한수의학회지
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• 제32권3호
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• pp.429-434
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• 1992

This study was carried out to develop an effective liquid-phase double antibody enzyme immunoassay for determining of progesterone. The optimum conditions of assay system, 1st and 2nd antibodies, enzyme conjugate, and time reaction were invested. The bovine plasma progesterone level in dairy cattle and korean native bulls were also analyzed. The results obtained were as follows; 1. The reproducibility of petroleum ether was superior to that of ethyl ether as extract solvent of progesterone in plasma. 2. The optimum dilution rate of 1st and 2nd antibody was 30,000 and 10 times, respectively. Affer the reaction of enzyme conjugate to progesterone 1st antibody, and then 2nd antibody competition reaction was enough for over 1hr. 3. Average plasma progesterone level in 4 pregnant and 9 nonpregnant Holstein was $2.5{\pm}0.5$ and $0.7{\pm}0.2ng/m{\ell}$, respectively. Average plasma progesterone level of 10 Korean native bulls was $0.1{\pm}0.001ng/m{\ell}$ From these results, by using liquid phase double antibody enzyme immunoassay for progesterone is applicable to detect of early pregnancy diagnosis, factorial analysis of reproductive disorder, and also reproductive physiological function such as monitoring of cyclicity during the post-partum period.

• 한국발생생물학회지:발생과생식
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• 제9권2호
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• pp.65-83
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• 2005

면역측정법의 주요 발전 단계를 3가지로 구분한다. 즉 첫 발전 단계(제 1세대)는 방사성 호르몬(방사선 추적자, radiolabeled analyte marker)을 이용한 길항적 측정방법의 개발과 보급이다. 두 번째(제 2세대)는 단가 항체(monoclonal antibody, McAb)를 추적자로 만들고, 비방사성 표지자를 이용하여 비경쟁적 초감도의 측정방법이 면역진단 분야에 적용되는 단계이다. 세 번째(제 3세대)의 발전은 최소량화, 칩을 이용하는(chip-based) microarray의 방법을 응용하여, 한 시료에서 여러 가지 생리활성물질 즉 호르몬의 동태를 파악하는 초감도 다변량분석법(simultaneous ultrasensitive measurement)이 개발되고 보급되는 단계라 할 수 있다. 제 1세대의 방사면역측정법(radioimmunoassay, RIA)을 거쳐 제 2세대 비방사면역측정법(non-isotopic immunoassay methods, NIA)이 모두 장 단점이 비교되고, 각각의 특수성을 가진 측정법이 정립되고, 시장에서의 우열이 정리되고 있는 시점이다. 이미 제 3세대(Chip/microarray-based multianalyte ligand assays)가 매우 빠르게 개발되고 있어 새로운 전환기를 맞고 있다고 판단된다. 그러나 본 종설에서는 일차로 제 2세대를 정리하고, 이어 새로운 전환점의 측정법을 소개하고자 한다.