• 한국균학회지
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• 제23권4호통권75호
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• pp.332-339
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• 1995

Fomitella fraxinea의 자실체로부터 중성염 용액을 사용하여 다당류 FCW를 추출하였다. FCW를 이온 교환 크로마토그라피하여 FCW-I 과 FCW- II로 분획하였고, 이 두 분획을 겔 여과 크로마토그라피하여 흰색 분말의 FCW-Ia와 갈색 분말의 FCW-IIa를 각각 얻었다. FCW-Ia 와 FCW-IIa 의 균질성을 HPLC 와 GPC로 확인하였고, 그들의 분자량을 각각 15,000 과 8,700 으로 결정하였다. FCW-Ia에는 항암 면역 증강 활성이 없었고, FCW-IIa는 높은 활성을 나타냈다. 두 분획에서 소량의 uronic acid 가 검출되었다. GC에 의한 단당류 분석 결과 FCW-Ia는 fucose, galactose, 그리고 mannose를 갖고 있으며 그 몰비는 19.5 : 63. 2 : 25.0이었다. 단백질은 FCW-Ia에서 검출되지 않았다. 한편 FCW-IIa의 주 단당류는 glucose, galactose 및 mannose 로서 그 비는 1.0 : 0.3 : 0.4이었으며, 단백질을 0.4% 함유하고 있다. 아미노 산 분석 결과 높은 양의 glutamic acid이 검출되었다.

• 대한본초학회지
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• 제23권2호
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• pp.151-157
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• 2008

Objectives : The purpose of this study is to investigate the biological Effect of multi-herbal drug 'KOCO-P1' on mouse macrophage Raw 264.7 cells. Methods : Multi-herbal drug 'KOCO-P1' was composed of Ginseng Radix, Astragali Radix, Polygonati Rhizoma, Liriopis Tuber, and Scrophulariae Radix. Cytotoxicity and cytoprotective activity of K0C0-P1 was verificated by MTT assay. And antioxidative effect of K0C0-P1 against EtOH, Nicotine was inspected by Hydroperoxide assay. Results : K0C0-P1 showed no cytotoxicity on RAW 264.7 cells for 24, 48, 72 hours. KOCO-P1 at 200, 100, and 50 ug/mL reduced the production of H202 in Raw 264.7 cells by EtOH. KOCO-P1 at 50 ug/mL reduced the production of H202 in Raw 264.7 cells by Nicotine. Conclusions : KOCO-P1 could be supposed to have antioxidative effect on macrophage with no cytotoxicity.

• 동의생리병리학회지
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• 제16권5호
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• pp.932-937
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• 2002

The purpose of this research was to investigate the effect of Yonggak-san (YGS) on the immune reaction and apoptosis of leukemia cells. Administration of YGS(500 mg/kg) enhanced proliferation of splenocytes, thymocytes and mesenteric lymph node cells, and also YGS accelerated subpopulation of splenic Band T, thymic T and mesenteric lymph node-T lymphocytes, especially significantly increased CD4+-TH cells in BALB/c mice. YGS accelerated phagocytic activity and production of nitric oxide in peritoneal macrophages. YGS induced apoptosis of transplanted-L1210 cells in vivo, increased apoptotic cell death of cultured-L1210 and/or Molt4 human leukemia cells, decreased of mitochondrial transmembrane potential of both cells in vitro. These results suggest that YGS have an immune-regulatory effect and anti-cancer property.

• 동의생리병리학회지
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• 제22권6호
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• pp.1495-1499
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• 2008

The purpose of this study is to investigate the effect of water extract from Artemisiae Argi Folium (WAAF) on hydrogen peroxide production within mouse macrophage Raw 264.7 Cells treated with gallic acid, EtOH, LPS, and acetaminophen. WAAF (0${\sim}$400 ug/mL) was treated with gallic acid, EtOH, LPS, acetaminophen. And the intracellular productions of hydrogen peroxide were measured by dihydrorhodamine 123 (DHR) assay. WAAF showed the restoration of the intracellular productions of hydrogen peroxide which were reduced by gallic acid, EtOH, LPS, and acetaminophen in Raw 264.7 Cells. WAAF could be supposed to have the immunological activity related with macrophage's oxidative burst.

• 미생물학회지
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• 제28권2호
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• pp.114-119
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• 1990

효모 Saccharomyces cerevisiae의 염색체상에 존재하는 superkiller 유전자인 SKIB 유전자를 cloning 시켜 ski 변이 주내에서 발현시켰다. 이 유전자의 C-말단부위에 E. coli의 tacZ 구조 유전자를 융합시켜 효모와 E. coli의 shuttle vector인 pSR605를 제조하고 이를 효모에 형질전환 시킨 후 나타나는 $\beta$-galactosidase의 융합단백질을 확인할 수 있었다.

• 생약학회지
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• 제21권2호
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• pp.137-141
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• 1990

The effects of various fractions from the aerial parts of Echinosophora koreensis Nakai on the antimutagenic and the immuno-regulating activities were evaluated by in vivo bone-marrow micronucleus test and HA titer reaction. No significant suppressive effects of these extracts and echinoisosophoranone were shown on cyclophosphamide-induced micronuclei, but HA titers were significantly enhanced in ether and BuOH extract treated-group. Tetracosanol and docosanol were isolated from the ether extract of this plant.

• Advances in Traditional Medicine
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• 제5권1호
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• pp.48-56
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• 2005

We studied the protective effect of Echinacea purpurea against radiation by evaluating changes in the peripheral blood cell count and peripheral blood antioxidant activity. Echinacea purpurea administration had a suppressive effect on radiation-induced leukopenia, especially on lymphocytes and monocytes and resulted in a faster recovery of blood cell counts. Mouse peripheral blood antioxidant activity was increased by Echinacea purpurea, and a relationship between the suppressive effect on radiation-induced leukopenia and the antioxidant effect was suggested.

• 한국식품저장유통학회지
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• 제12권5호
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• pp.496-500
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• 2005

홍경천(Rhodiola dumulosa)을 기능성 식품소재로 개발하기 위하여 홍경천(뿌리)을 80% 메탄올로 열수 추출하여 용매 분획한 후 이들에 대한 항산화, 암세포 성장 억제 및 대식세포의 면역활성 등과 같은 기능성을 조사하였다. 홍경천 메탄올 추출물의 헥산, 에틸 아세테이트, 부탄올, 메탄올 및 물 분획물의 수소공여능은 100 ${\mu}g/mL$의 농도에서 각각 28.30, 53.21, 35.48, 42.64 및 21.14% 로서 에틸아세테이트 분획물이 가장 높았으며, 이는 합성 항산화제인 BHT와 비슷한 항산화 효과를 나타내었다. A549 및 SW480 와 같은 암세포에 용매 분획물 1, 10 및 100 ${\mu}g/mL$의 농도로 48시간 처리한 결과 에틸아세테이트 분획물이 다른 분획물에 비하여 암세포주의 성장을 강하게 억제하였으며, 그 활성은 농도 의존적으로 나타났다. 또한 100 ${\mu}g/mL$의 농도로 48시간 동안 에틸아세테이트 분획물을 암세포에 처리시 처리구에서의 암세포는 뚜렷한 세포수의 감소와 함께 심한 형태학적 변화가 관찰되었다. 대식세포주(RAW 264.7)에 1, 10 및 50 ${\mu}g/mL$의 농도로 용매 분획물을 처리한 후 NO의 생성량을 측정한 결과 10 μg/mL 이상의 농도에서 메탄올 분획물에서 NO 생성을 강하게 유도하였다. 따라서 본 결과는 홍경천의 기능성 식품소재로의 활용 가능성을 시사한다.

• BMB Reports
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• 제39권1호
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• pp.84-90
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• 2006

During our search for macrophage stimulating compounds from medicinal plants, we isolated biopolymers from Acanthopanax sessiliflorus. Isolated fraction AS-5 showed maximum potential, and stimulated lysosonal enzymatic activity by 230% at $300\;{\mu}g/ml$. The nitric oxide (NO) producing ability of AS-5 $100\;{\mu}g/ml$ was $58\;{\mu}M$ when treated with interferon-$\gamma$ and lipopolysaccharide $20\;{\mu}g/ml$. The lymphocyte proliferating effects of isolated biopolymer fractions were also investigated. Highest lymphoproliferative activity (a 2.8-fold enhancement compared to saline treated group was exhibited by AS-3 at $200\;{\mu}g/ml$ followed by AS-5 and AS-6. The AS-3 fraction stimulated only T-lymphocytes and had little or no effect on B-lymphocyte proliferation. Partially methylated alditol acetates were prepared to elucidate the glycosyl linkage-compositions of the AS-3 and AS-5 biopolymers, and were analyzed by GC-MS. The AS-3 and AS-5 biopolymer fractions were found to contain 2,3,4-tri-O-methyl-D-glucitol, 2,3,4-tri-O-methyl-D-galacitol 3,4,6-tri-O-methyl-galacitol, 2-O-methyl-arabinitol and 2,4,6-tri-O-methyl-D-glucitol, 2,3,6-tri-O-methyl-D-galacitol linkages, respectively.

• 약학회지
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• 제52권6호
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• pp.494-499
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• 2008

The role of antibody in the fungal infections is controversial. However, our previous reports showed a certain epitope in Candida albicans cell wall (CACW) induces protective antibody. A major problem is that the epitope isolation requires tremendous time with high cost. This aspect led us to investigate a simple way inducing protective antibodies against C. albicans. In the present study, we determined if $18{\beta}$-glycyrrhetinic acid ($18{\beta}$-GA) from Glabrae Radix (a family of Leguminosae) has immunoadjuvant activity. Data displayed that the $18{\beta}$-GA suppressed proliferations of both T- and Blymphocytes at high concentrations, whereas below 20 ${\mu}M$ concentration the compound supported the proliferations. These observations indicate that $18{\beta}$-GA has immunoregulatory activity. Based on this observation, an immunoadjuvant effect was examined at the low concentration. Results from animal experiments showed that CACW combined with or without $18{\beta}$-GA produced the anti-C. albicans antiserum in mice. Nevertheless, the CACW combined with $18{\beta}$-GA formula only protected mice against disseminated candidiasis (P<0.05). These data implicate that $18{\beta}$-GA has immunoadjuvant activity, which may provoke the CACW antigen to induce protective antibody. Currently, we are investigating possible mechanism of how the $18{\beta}$-GA provokes such protective immunity against the disseminated disease.