• The Korean Journal of Food And Nutrition
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• v.11 no.3
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• pp.319-322
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• 1998

The whole cell of alkalophilic Streptomyces sp. B-2 which produce glucose isomerase was immobilized by entrapment method for the effective production of high fructose syrup. The highest immobilized activity was achieved when the enzyme was bound to 2% $textsc{k}$-carrageenan. Immobilized glucose isomerase the pH optimum was about pH 7.5~8.5. Immobilization of alkalophilic Streptomyces sp. B-2 on 2% $textsc{k}$-carrageenan at 7$0^{\circ}C$ showed an increase in glucose isomerase activity. GI activity of immobilized cells was maximum Co2+ concentration 10-3M, Mg2+ concentration 10-3M.

• Biotechnology and Bioprocess Engineering:BBE
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• v.3 no.2
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• pp.96-102
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• 1998

Primary hepatocytes of small animals such as rat and rabbit were often used for the study of extracorporeal liver support systems. Freshly isolated rat hepatocytes form spheroids within tow days when cultivated as suspension in spinner vessels. These spheroids showed enhanced liver specific functions and more differentiated morphology compared to hepatocytes cultured as monolayers However, shear stress caused by continuous agitation deteriorated spheroids gradually. In this work we immobilized spheroids to prolong liver specific activities. First, hepatocyte spheroids were suspended in collagen solution containing calcium chloride and then dropped into alginate solution. A thin layer of calcium alginate was formed around the droplet and then was removed after the inner collagen was gelled by treatment of sodium citrate buffer. Spheroids embedded in collagen-gel bead maintained liver specific functions such as albumin secretion rate longer than hepatocyte spheroids exposed to shear stress. Therefore, we suggest that this immobilization technique may offer an effective long-term hepatocyte cultivation and facilitase the development of a bioartificial liver support device.

• 한국생물공학회:학술대회논문집
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• 2000.11a
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• pp.536-539
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• 2000

A simple and effective method has been developed for the immobilization of the cell on polyurethane foam. Two types of commercially available polyurethane foam and Hydro-filt were tested. The ultimate purpose of the process is to produce low-cost materials for hydrogen sulfide removal which are being increasingly used for industrial application. Effect of several parameters were studied on the cell loading. These parameters were type, size, and amount of polyurethane foam. MC-70 was the best immobilization material of three type of carriers, and optical particle size was $5{\sim}8mm$ and amount of polyurethane foam was 8g/L.

• Proceedings of the Korea Technical Association of the Pulp and Paper Industry Conference
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• 2000.04a
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• pp.103-110
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• 2000

This study was done in order to increase production speed using coater dryer in maximum capacity without print mottle. The aim of this study was to measure coating color immobilization content(CCIC) and to decrease CCIC through coating color optimization. The final goal of this study was the prevention of print mottle in maximum drying condition by CCIC control. As a result the measuring method of CCIC was set by brightness transition and the effective factors in pigments and binders were defined. With color optimization considered this factors, CCIC was decreased by 4%. Through this CCIC decrease and modification of coater utility, the print mottle was prevented even though using coater dryer in maximum.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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• 2006.06a
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• pp.410-411
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• 2006

High throughput analysis using a DNA chip microarray is powerful tool in the post genome era. Less labor-intensive and lower cost-performance is required. Thus, this paper aims to develop the multi-channel type label-free DNA chip and detect SNP (Single nucleotide polymorphisms). At first, we fabricated a high integrated type DNA chip array by lithography technology. Various probe DNAs were immobilized on the microelectrode array. We succeeded to discriminate of DNA hybridization between target DNA and mismatched DNA on microarray after immobilization of a various probe DNA and hybridization of label-free target DNA on. the electrodes simultaneously. This method is based on redox of an electrochemical ligand.

• Applied Science and Convergence Technology
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• v.25 no.3
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• pp.61-65
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• 2016

Here, the rapid detection of Salmonella typhimurium by a portable surface plasmon resonance (SPR) biosensor in which the beam from a diode laser is modulated by a rotating mirror is reported. Using this system, immunoassay based on lipopolysaccharides (LPS)-specific monoclonal anti-Salmonella antibody was performed. For the purpose of orientation-controlled immobilization of antibodies on the SPR chip surface, the cysteine-mediated immobilization method, which is based on interaction between a gold surface and a thiol group (-SH) of cysteine, was adopted. As a result, using the portable SPR-based immunoassay, we detected S. typhimurium in the range from 10^7 CFU/mL to 10^9 CFU/mL within 1 hour. The results indicate that the portable SPR system could be potentially applied for general laboratory detection as well as on-site monitoring of foodborne, clinical, and environmental agents of interest.

• Proceedings of the Korean Vacuum Society Conference
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• 2013.08a
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• pp.268.1-268.1
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• 2013

Immobilization of conducting nanoparticles on a nanogap comprising two electrodes spaced at a distance comparable to the particle size can be used as a simple and sensitive method of detecting the particles. In this work, we have examined the performance of the nanogap devices in the measurement of metallic nanoparticles, particularly gold nanoparticles (Au NPs). Detection of pM-level Au NPs in an aqueous suspension was quite straightforward irrespective of the existence of non-conducting materials. Speed of detection or the time necessary for the completion of the measurement, however, was strongly dependent upon the immobilization process. Active trapping process was found to be much more efficient and also effective in the detection of nanoparticles than its passive counterpart.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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• 2004.07b
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• pp.845-848
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• 2004

High throughput analysis using a DNA chip microarray is powerful tool in the post genome era. Less labor-intensive and lower cost-performance is required. Thus, this paper aims to develop the multi-channel type label-free DNA chip and detect SNP (Single nucleotide polymorphisms). At first, we fabricated a high integrated type DNA chip array by lithography technology. Various probe DNAs were immobilized on the microelectrode array. We succeeded to discriminate of DNA hybridization between target DNA and mismatched DNA on microarray after immobilization of a various probe DNA and hybridization of label-free target DNA on the electrodes simultaneously. This method is based on redox of an electrochemical ligand.

• Proceedings of the Korean Magnestics Society Conference
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• 2014.05a
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• pp.148-148
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• 2014

• 한국생물공학회:학술대회논문집
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• 2005.10a
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• pp.741-741
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• 2005