• Applied Microscopy
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• 제52권
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• pp.4.1-4.11
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• 2022

Field emission scanning electron microscopy (FESEM) is an essential tool for observing surface details of specimens in a high vacuum. A series of specimen procedures precludes the observations of living organisms, resulting in artifacts. To overcome these problems, Takahiko Hariyama and his colleagues proposed the concept of the "nanosuit" later referred to as "NanoSuit", describing a thin polymer layer placed on organisms to protect them in a high vacuum in 2013. The NanoSuit is formed rapidly by (i) electron beam irradiation, (ii) plasma irradiation, (iii) Tween 20 solution immersion, and (iv) surface shield enhancer (SSE) solution immersion. Without chemical fixation and metal coating, the NanoSuit-formed specimens allowed structural preservation and accurate element detection of insulating, wet specimens at high spatial resolution. NanoSuit-formed larvae were able to resume normal growth following FESEM observation. The method has been employed to observe unfixed and uncoated bacteria, multicellular organisms, and paraffin sections. These results suggest that the NanoSuit can be applied to prolong life in vacuo and overcome the limit of dead imaging of electron microscopy.

• 수산해양교육연구
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• 제28권6호
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• pp.1581-1590
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• 2016

In order to reduce the risk of death from hypothermia for the fishing vessel's crew at sea, this paper suggests that the criteria of equipment of fishing vessels should be revised for fishing vessels engaged in coastal and inshore fisheries to be equipped with the immersion suit. The criteria of equipment of fishing vessels for immersion suits was amended to reflect the sinking of No.501 Oryongho but it was only reflected in ocean fisheries at Bering sea and Antarctic ocean that immersion suits must be provided with the same number of the total number of crew on board. Therefore, this paper analyses the relationship between maritime accident of fishing vessels and weather condition based on sea water temperature to find out the risk of hypothermia and also compares the international conventions(SFV Protocol 1993, Cape Town Agreement 2012, STCW-F 1995 and SOLAS 1974) and domestic law concerning criteria of equipment of fishing vessels. As a result, fishing vessels engaged in coastal and inshore fisheries are exposed to the risk of hypothermia when they are in distress and the criteria of equipment of fishing vessels should be amended to provide the immersion suits in accordance with the revision trend of international conventions.

• 품질경영학회지
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• 제48권3호
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• pp.433-451
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• 2020

Purpose: The purpose of this study is to investigate how the type of virtual reality content production affects the interaction and immersion that is a virtual reality characteristic, and to provide the platform companies and content producers with the basic information necessary to provide the production and service of content suitable for VR characteristics. Methods: Based on the data collected in the survey, multiple regression analysis and hierarchical regression analysis were used. The measurement tools used in this study were studied through three-dimensional composition, including the characteristics and interaction and immersion of content produced in 2D, 3D and 360°, which are elements of virtual reality characteristics. Results: The results of this study are as follows. Among the types of content production, content produced with 2D and 360 technologies was found to affect immersion, while content produced in 3D affected interaction. Motion sickness has been investigated to affect both immersion and interaction. Conclusion: Service-provided platform enterprises and content-making enterprises should consider content-making and providing services that suit service characteristics and purposes, taking into account the characteristics of interaction and immersion in content-making investment and service delivery.

• 한국염색가공학회지
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• 제33권4호
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• pp.317-326
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• 2021

In this study, we investigated for Bio-EPDM foam with oyster shell surface modified earth coupling agent. Experiments were carried out to confirm the bio-EPDM/Oyster shell foam applying content of earth coupling agent. The cure characterization were evaluated by measuring the mooney viscosity and oscillating disc rheometer (ODR). Mechanical properties such as hardness, tensile strength, elogation at break and tear strength were measured, and changes of mechanical properties were also evaluated after immersion in NaCl solution. In addition degree of volume change was measured after immersing the Bio-EPDM foam in NaCl solution and the low-temperature permanent compression set was evaluated at 4℃. To evaluate the low-temperature characteristics of Bio-EPDM/Oyster shell, the glass transition temperature was measured using Differential Scanning Calorimeter (DSC). As a result as the content of the earth coupling agent increased up to 3phr, the crosslinking density and mooney viscosity increased, and the mechanical properties and low-temperature permanent compression set improved, but from 4phr, it was rather decreased. The change in the glass transition temperature was insignificant, and the foam cell appeared to be uniform when the earth coupling agent was applied.