• 한국약용작물학회지
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• 제19권5호
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• pp.313-318
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• 2011

This study was carried out to utilize the byproducts (flower, immature and mature berry, leaf and stem) of ginseng. Yield of byproducts were $32.7{\pm}9.8g$ in flower, $68.2{\pm}2.2g$ in immature berry, $48.5{\pm}4.3g$ in mature berry, $316.2{\pm}20.5g$ in leaf, and $296.6{\pm}15.4g$ in stem per $3.3m^2$ ($180{\times}90cm$, ginseng root $675.5{\pm}35.7g$/drybasis. The total saponin contents of ginseng byproducts and root are $52.36{\pm}1.24$, $68.71{\pm}1.98$, $168.89{\pm}0.57$, $68.26{\pm}1.32$, $7.85{\pm}0.61$ and $35.08{\pm}0.96$ mg/g, respectively. The main ginsenoside of all byproducts was Re and the highest content was $132.23{\pm}1.56$ mg/g in mature berry. But flower and berry was not detected Rf and Rh1, respectively. Total polyphenolic compound content on mature berry was the highest, $2.242{\pm}0.140%$, after, immature berry > leaf > flower > root > stem order. The DPPH radical scavenging activity on mature berry was the highest, $0.115{\pm}0.004$ mg/mL($IC_{50}$), and the others were the same order of polyphenolic compound and ginsenoside content on byproducts.

• 대한장애인치과학회지
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• 제12권2호
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• pp.72-76
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• 2016

저자는 뇌병변 장애와 보행장애로 반복적인 외상성 치아손상을 받은 환아의 치료를 시행하였다. 최근 실활된 미성숙 영구치의 계속된 치근 형성을 위해 재생적 근관치료 술식이 많이 시도되어 성공적인 결과를 보이고 있다. 하지만 장애로 인해 반복적인 외상의 위험을 가진 환아에서 치수조직의 재생 보다는 치근단형성술을 통한 치근단의 폐쇄와 근관 충전이 비용과 장기적인 예후의 측면에서 유리한 치료방법이 될 수 있음을 확인하였다.

• 한국환경농학회지
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• 제38권2호
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• pp.89-95
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• 2019

BACKGROUND: Liquid pig manure (LPM) has been used as an alternative for conventional fertilizers on some gramineous crops. However, its chemical properties varied widely depending on the degree of the digestion. A pot experiment was conducted to determine the responses of persimmon trees to immature (not well-digested) LPM application. METHODS AND RESULTS: Ten application levels of immature LPM, consisted of a total of 3 to 30 L in 3-L increment, were applied during summer to 5-year-old 'Fuyu' trees grown in 50-L pots. Increasing the LPM application rate caused defoliation, wilting, and chlorosis in leaves. When applied with the rate of 3 L during summer, the tree produced small fruits with low soluble solids and bore few flower buds the following season, indicating insufficient nutritional status. In trees applied with the LPM rates of 6~12 L, both fruit characteristics and above-ground growth of the trees appeared normal but some roots were injured. However, application of higher LPM rates than 27 L resulted in small size, poor coloration, or flesh softening of the fruits the current season. Furthermore, the high LPM rates caused severe cold injury in shoots during winter and weak shoot growth the following season. It was noted that the application of higher LPM rate than 9 L damaged the root, even though above-ground parts of the tree appeared to grow normally. CONCLUSION: The results indicated that an excessive immature LPM application could cause various injuries on leaves, fruits, and the roots in both the current and the following season.

• Journal of Periodontal and Implant Science
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• 제38권2호
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• pp.225-230
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• 2008

Purpose: Many clinical studies have reported that higher success rates are achieved with teeth that have immature roots than other autotransplanted teeth that have more immature root. However, based on date published recently, the success rate of autotransplantation of teeth with complete root formation was higher. The purpose of this study was to examine the long term(2 to 6 years follow-up) success rate of autotransplantation of third molar with complete root formation and to discuss some conditions and prerequisites for success. Materials and Methods: 26 sites of 24 patients aged 26 to 55 (mean age 40.8) were autotransplanted with third molars with complete root formation. These cases were followed for 2 to 6 years after surgery. The success criteria included (1) no discomfort during functioning (2) absence of progressive root resorption and alveolar bone resorption. Result: Of 26 teeth 5 teeth were failed, therefore success rate is 81%(21/26 teeth). The results suggested that higher success rate is acquired from (1) extraction socket due to dental caries (2) mandibular recipient site (3) patient younger than 40 years old. Autotransplantation of third molar to replace molars with advanced periodontal disease also showed considerably high success rate(84%). Conclusion: With appropriate case selection, autotransplantation of third molar with complete root formation remains a viable alternative for replacing a missing molar tooth.

• 대한소아치과학회지
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• 제34권3호
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• pp.454-460
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• 2007

성장 발육중인 어린이나 청소년에서 치아에 가해지는 외상성 손상은 성인에 비하여 흔히 일어난다. 외상이 일어난 후부터 적절한 치료가 시행될 때까지 소요된 시간은 특히 예후를 결정하는데 중요한 요소이며, 외상성 치아 손상을 입은 환자를 검사하고 치료하는데 사용되는 시간이 제한되어 있기 때문에, 만약 적절한 치료가 시간 내에 시행되지 않는다면 환자에게 심각한 결과를 초래할 수 있다. 이전 연구들에 의하면, 외상의 발생율은 8-10세 아이들에게서 가장 흔하고, 가장 많이 이환된 치아는 상악 영구 중절치이며, 남자의 경우가 여자보다 2:1로 빈도가 높았다. 상악 중절치의 치근 완성시기는 평균 10세 정도로 알려져있다. 외상성 손상에 이환된 호발 연령대의 상악 영구 중절치는 대부분 발육중인 미성숙된 치아로, 혈류공급이 왕성하여 손상시 재혈관화에 의한 치유가 비교적 쉽게 일어날 수 있어 치수 생활력에 대한 긍정적인 예후가 기대될 수 있다. 본 증례는 외상성 손상을 받은 미성숙 상악 영구 중절치의 치근파절 치험례로, 통상적인 근관치료를 하지 않고 보존적인 술식을 통하여 치아의 생활력을 유지할 수 있었기에 그 치료 경과 및 결과를 보고하는 바이다.

• 한국작물학회지
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• 제40권3호
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• pp.308-313
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• 1995

본 시험은 미숙종자의 배배양을 이용한 보리의 세대촉진을 위하여, 재배조건(15/10, 25/15$^{\circ}C$)과 등숙기간에 따른 미숙종자의 형질을 조사하고 이들 형질들간의 상호 관계를 구명함과 동시에, 미숙종자의 배배양시 유묘의 생육 반응을 검토하였던 바, 그 결과를 요약하면 다음과 같다. 1. 출수 후 21일이 경과한 미숙종자의 배장은 이삭의 하부보다는 중상부가, 저온(15/10$^{\circ}C$)보다는 고온(25/15$^{\circ}C$) 조건에서 길었는데, 특히 저온 조건의 하부에서 짧았다. 그러나 부위별 차이보다는 생육 온도 조건에 따른 차이가 컸다. 2. 배배양 후 유아장과 근수 및 근장은 고온의 경우 이삭 부위별 차이가 적었으나, 저온조건에서는 중, 상부보다 하부의 생육이 현저히 불량하였다. 3. 등숙 기간에 따른 배장의 변리는 고온 조건에서 21일경에 최장에 이르러 더이상 신장하지 않았지만 저온 조건에서는 29일까지도 계속적으로 신장하여 저온 조건의 29일배가 고온 조건의 17일배와 유사한 경향치를 보였다. 4. 고온 조건에서 17일이 경과한 배의 배장은 입폭, 유아장, 근수 및 근장과 정의 상관이 인정되었으나, 21일이 경과한 것은 어느 형질과도 상관이 인정되지 않았다. 5. 미숙종자의 배배양을 이용한 세대촉진시에는 25/15$^{\circ}C$의 고온 조건에서 출수후 17일이 경과한 배를 이용하는 것이 적당할 것으로 보였다.

• Journal of Plant Biology
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• 제33권4호
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• pp.271-276
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• 1990

Our study was carried out for plant regeneration via somatic embryogenesis from immature seeds of Bletilla striata. The highest frequency of embryogenic callus formation was obtained from the immature seeds (at 150 days after pollination) cultured on Hyponex and VW medium supplemented with 3 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D) and 1 mg/l kinetin under the dark condition. Multiple somatic embryos were induced when embryogenic callus was transferred to VW medium without growth regulators under continued illumination. Somatic embryos were observed histologically with scanning electron microscopy. Regeneration of Bletilla striata was obtained from somatic embryos with a well-defined scutellum and coleoptile as well as with one or more shoot primordia and root primordia. We think that these methods for orchid multiplication must be useful to access clonal propagation of orchids.

• 대한소아치과학회지
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• 제26권2호
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• pp.437-445
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• 1999

외상받은 치아의 치수 생활력을 조기에 진단하는 것은 매우 중요하다. 그러나 대부분의 경우 치수생활력을 조기에 알 수 없으며, 특히 미완성 치근단을 가진 치아에서는 치수생활력 검사의 신뢰도는 더욱 낮아진다. 본 연구는 미완성 치근단을 가진 치아를 대상으로 하여 치근단 완성도에 따른 Laser Doppler Flowmeter(이하 LDF)의 측정치 차이와 발육단계에 따른 각각 LDF 측정 기준치를 알아 보고자 하였다. 이를 위하여 23세에서 24세 성인 남녀 20명 및 7세에서 8세 취학 아동중 치근단공의 크기가 2mm이상인 아동 20명, $1{\sim}2mm$인 아동 20명의 상악 좌우중절치를 대상으로 LDF검사를 시행하였다. LDF는 안정된 signal이 나타날 때 까지 5분이상 관찰하였으며, 안정상태 도달후 5분이상 측정하였다. 모든 반응인자의 정규분포를 확인한 다음, 각 시험군간의 LDF측정치를 ANOVA test를 이용하여 비교하였고, 동일대상에서 좌우 치아간의 비교는 paired-t test를 이용하여 다음과 같은 결론을 얻었다. 1. LDF측정치에 있어 미완성 치근단을 가진군이 완성된 치근단을 가진 군에 비해 높게 나타났지만 통계학적으로 유의한 차이는 나타나지 않았다 (p>0.05). 2. 미완성 치근단을 가진 군간의 비교시 LDF측정치에 있어 통계학적으로 유의한 차이는 나타나지 않았다 (p>0.05). 3. 각 실험군내에서 좌우중절치간의 비교시 LDF측정치에 있어 통계학적으로 유의한 차이는 나타나지 않았다 (p>0.05).

• Journal of Ginseng Research
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• 제31권1호
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• pp.14-22
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• 2007

As the zygotic embryo of North American ginseng (Panax quinquefolius L.) matured during stratification over 203 days it grew from 0.75 to 5.2 mm. Embryo excision and culturing on media containing different concentrations of two growth regulators, gibberellic acid ($GA_3$, 1 to 10 ${\mu}M$) and benzyladenine (BA, 1 to 5 ${\mu}M$), during stratification, showed that shoot and root number and the shoot, root and cotyledon length increased with increased stratification time. Gibberellic acid was the more effective growth regulator for increasing shoot and root number and shoot, root and cotyledon lengths. Immature embryos (stratified for up to 63 days) needed growth regulators for further development. Cultures on $GA_3$ at the last culture date (stratified for 203 days) when embryos were mature, produced multiple shoots but there was no effect of $GA_3$ concentration. Benzyladenine inhibited shoot and root growth regardless of embryo stratification. Growth regulators had little effect on cotyledon length of mature embryos. Embryos cultured on $GA_3$ combined with BA were green on all culture dates whereas greening in the control and BA treatments increased with culture date. The BA treatments induced 100% swelling of the embryos on the final culture date while in the control and $GA_3$ treatments there was no swelling. There was little or no curling in the control and BA treatments and a linear decrease in curling with culture date in the $GA_3$ and $GA_3$ + BA treatments.

• Restorative Dentistry and Endodontics
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• 제45권3호
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• pp.29.1-29.9
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• 2020

This study describes the clinical steps taken in the treatment of a patient who had an avulsed right upper central incisor that presented with incomplete root development and chronic apical periodontitis. A 7-year-old boy was referred from a private dentist to a dental office specializing in endodontics. The tooth had remained in a dry environment for 20 minutes, and tooth replantation was performed at an emergency appointment. After clinical and radiographic examinations, root canal decontamination was performed, followed by several changes in intracanal calcium hydroxide medication. Blood clot formation was attempted, but bleeding within the root canal was insufficient; therefore, we opted for an intracanal medication change to stimulate mineralized tissue formation in the apical region. Root obturation was performed 45 days after the last change of intracanal medication, and clinical, radiographic, and tomographic follow-up examinations were performed at 3, 6, 18, and 40 months after the endodontic intervention. The increase in thickness and length of the root structure and the absence of root resorption were verified through follow-up examinations. Therefore, it was concluded that the procedures used were successful for tooth replantation.