• Journal of Life Science
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• v.15 no.3 s.70
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• pp.317-322
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• 2005

The cycloinulooligosaccharide fructanotransferase (CFTase) gene (cft) from Paenibacillus macerans was subcloned into an E. coli-yeast shuttle vector, pYES2.0, resulting in pYGECFTN. The plasmid pYGECFTN (8.6 kb) was introduced into Saccharomyces cerevisiae SEY2102 cells and then the transformants were selected on the synthetic defined media lacking uracil. The cft gene expression in yeast transformant was demonstrated by the analyses cyclofructan (CF) spots on thin-layer chromatogram. The recombinant CFTase was not secreted into the medium and localized in the periplasmic space. The production of CF was observed after 5 min of the enzymatic reaction with inulin. The optimun pH and temperature for CF production were found to be at pH 8.0 and $45^{\circ}C$, respectively. Enzyme activity was stably maintained up to $55^{\circ}C$. The CF was produced from all inulin sources and was most efficiently produced from dahlia tubers and Jerusalem artichokes.

• The Journal of the Korea Contents Association
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• v.17 no.1
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• pp.634-661
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• 2017

This study examines the procedure of successful novel adaptation. It is well known from precedent studies that narrative structure of novels should change forms to suit the media characteristics of films. But, the changing forms of narrative structure is not a sufficient condition of successful novel adaptation, but a necessary condition. A successful adaptation could be completed with filmic expressions on the presented narrative structure. The core of filmic expression is cinematic language which means the composition and array of image and sound. The novel, Kirishma Thing deals with everyday life of high school students and it consists of six stories which are narrated by one student each in first person point of view. The film, Kirishma Thing implemented a different strategy. It reveals the same events several times to show many characters over in each person's point of view in the first half. In the second half, all the characters gathers at the rooftop of the school to have an unilinear narrative structure over one event. This film utilize all kinds of cinematic language to achieve these structures including the widescreen aspect ratio which exposes as many characters as possible in one shot, picture composition which shows the same event in a different point of view, contrast in lighting and music which differentiates and empowers the last sequence of the film.

• Journal of Broadcast Engineering
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• v.15 no.4
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• pp.474-486
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• 2010

In this paper, we propose a new method for detection moving object contour using spatial and temporal edge. In general, contour pixels of the moving object are likely present around pixels with high gradient value along the time axis and the spatial axis. Therefore, we can detect the contour of the moving objects by finding pixels which have high gradient value in the time axis and spatial axis. In this paper, we introduce a new computation method, termed as temporal edge, to compute an gradient value along the time axis for any pixel on an image. The temporal edge can be computed using two input gray images at time t and t-2 using the Sobel operator. Temporal edge is utilized to detect a candidate region of the moving object contour and then the detected candidate region is used to extract spatial edge information. The final contour of the moving object is detected using the combination of these two edge information, which are temporal edge and spatial edge, and then the post processing such as a morphological operation and a background edge removing procedure are applied to remove noise regions. The complexity of the proposed method is very low because it dose not use any background scene and high complex operation, therefore it can be applied to real-time applications. Experimental results show that the proposed method outperforms the conventional contour extraction methods in term of processing effort and a ghost effect which is occurred in the case of entropy method.

• Journal of Broadcast Engineering
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• v.22 no.1
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• pp.28-41
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• 2017

In this paper, the fast encoding algorithm in High Efficiency Video Coding (HEVC) encoder was studied. For the encoding efficiency, the current HEVC reference software is divided the input image into Coding Tree Unit (CTU). then, it should be re-divided into CU up to maximum depth in form of quad-tree for RDO (Rate-Distortion Optimization) in encoding precess. But, it is one of the reason why complexity is high in the encoding precess. In this paper, to reduce the high complexity in the encoding process, it proposed the method by determining the maximum depth of the CU using a hierarchical clustering at the pre-processing. The hierarchical clustering results represented an average combination of motion vectors (MV) on neighboring blocks. Experimental results showed that the proposed method could achieve an average of 16% time saving with minimal BD-rate loss at 1080p video resolution. When combined the previous fast algorithm, the proposed method could achieve an average 45.13% time saving with 1.84% BD-rate loss.

• The Journal of Engineering Geology
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• v.9 no.3
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• pp.187-206
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• 1999

A computer program to numerically predict the permeability tensor of fractured rocks is developed using information on discontinuities which Borehole Televiewer and Borehole Image Processing System (BIPS) provide. It uses orientation and thickness of a large number of discontinuities as input data, and calculates relative values of the 9 elements consisting of the permeability tensor by the formulation based on the EPM model, which regards a fractured rock as a homogeneous, anisotropic porous medium. In order to assess feasibility of the program on field sites, the numerically calculated tensor was obtained using BIPS logs and compared to the results of pumping test conducted in the boreholes of the study area. The degree of horizontal anisotropy and the direction of maximum horizontal permeability are 2.8 and $N77^{\circ}CE$, respectively, determined from the pumping test data, while 3.0 and $N63^{\circ}CE$ from the numerical analysis by the developed program. Disagreement between two analyses, especially for the principal direction of anisotropy, seems to be caused by problems in analyzing the pumping test data, in applicability of the EPM model and the cubic law, and in simplified relationship between the crack size and aperture. Aside from these problems, consideration of hydraulic parameters characterizing roughness of cracks and infilling materials seems to be required to improve feasibility of the proposed program. Three-dimensional assessment of its feasibility on field sites can be accomplished by conducting a series of cross-hole packer tests consisting of an injecting well and a monitoring well at close distance.

• Journal of Digital Convergence
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• v.13 no.7
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• pp.1-8
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• 2015

To identify the satisfaction level towards Gwangju regional TV broadcasting company's program, "I Like the Market" and the effect of market vitalization on the market merchants' public relationships, this study carried out survey targeting 256 merchants who are residing at the region. The research results demonstrated that the satisfaction level towards the program and market vitalization via the program exert significant effect on the public relationships. Specifically, satisfaction level towards the program for the vitalization of regional broadcast exert effect in the following order; devotion, relationship and reliability. Meanwhile, market vitalization exert positive effect on the relationship, reliability and devotion, in the order mentioned. This implies that the development of quality program for the regional residents plays an important role in the convergence of regional broadcast and in increasing broadcasting company's brand image. Accordingly, positive interaction via regional vitalization when it comes to the formation of regional broadcast program going forth may be needed for regional broadcast policies.

• The Korean Journal of Pain
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• v.8 no.2
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• pp.298-303
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• 1995

We have inserted epidural catheter for single or continuous injection of a drug for epidural analgesia. It is important to localize the tip of epidural catheter in appropriate site to acquire the most effective analgesia. In epidural block, we observed course and location of the tip of epidural catheter. Subject: 70 patients were divided into group I(non-injection of saline group during catheter insertion) and group II(injection group during catheter insertion). Group I included cervical(n=20), thoracic(n=10), and lumbar(n=20) epidural group. Group II, cervical(n=10), and lumbar(n=10) epidural group. Method: 19G FlexTip $Plus^{TM}$ Epidural Catheter ($Arrow^{(R)}$) was inserted 10cm cephaladly in epidural space with(group II) or without(group I) saline flushing. We observed course and location of the tip of epidural catheter by C-arm image intensifier during injection of contrast media ($Omnipaque^{(R)}$). Result: In group I, the number of tips of epidural catheters located within 2 cm from inserted site were: cervical 14/20(70%), thoracic 2/10(20%). lumbar 16/20(80%). In thoracic epidural blocks, tips of epidural catheters were more cephaladly located than with cervical and lumbar epidural blocks. With cervical epidural blocks, the number of tips of epidural catheters located within 2 cm from insertion site were less in group II than group I (20% vs. 70%). But no significant differences were noted between group I and group II with lumbar epidural block(90% vs. 80%). The number of tips of epidural catheters located around a predicted site were: cervical 2/20(10%), thoracic 4/10(40%), lumbar 0/20(0%) in group I, and cervical 2/10(20%), lumbar 1/10(10%) in group II. Conclusion: It was impossible to predict the exact location of tips of epidural catheters by measuring the inserted length without epidurogram. With many cases, tips of epidural catheters were located around the insertion site in lumbar epidural blocks, and in some cases around the predicted site in thoracic epidural blocks. The results suggests that epidural block should be done at a point near the required band of analgesia.

• Cartoon and Animation Studies
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• s.41
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• pp.133-152
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• 2015

Some artists and scientists in physics and animation originating from research on its form of expression thanks to the rapid development of the example in the late 20th century image production technology integrating existing media feature, perform a re-creation and pop culture content has been recognized as a key factor. animation of the modern emphasis is also commercial and artistic activities as show whether the artist can not be excluded that also target audience. The audience does not want only to receive offers simply 'seeing' and 'hearing' in the animation requires a more indirect mental met. the other side, the director should lead the audience to immerse myself in work as intended mystification induce the world. where a conflict occurs between the audience and the director and The director needs to have its troubleshooting point to 'Technology of the communication'. Which is reduced to 'How will tell,' is technology communication technologies that are abbreviated representations of animation director is accessible to the audience and it is a close relationship between the psychological aspect of audience. Because, the audience is reproduced in a limited space, but he called on the board of directors and the same time the screen, the audience located at reception and the director located at provide. It is given. led force is given to the director. for this reason, The director needs to pay attention to the psychological aspect of audience this can be explained based on psychoanalytic theory. In this paper, "How can you lie to the audience and the director is the same line?" put down logic that is the animation audience under the logic that takes place visually capture phenomenon "selective attention" and sub-concept of "goal-directed selection' and 'stimulus-driven capturel' for theory of psychology. also, Induction device to elicit selective attention of the audience accordingly, let's consider whether and how they apply in animation.

• The Journal of The Korea Institute of Intelligent Transport Systems
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• v.19 no.5
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• pp.97-105
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• 2020

Recently, for a smart city, there is a demand for a technology for acquiring traffic information using an intelligent road infrastructure and managing it. In the meantime, various technologies such as loop detectors, ultrasonic detectors, and image detectors have been used to analyze road traffic information but these have difficulty in collecting various informations, such as traffic density and length of a queue required for building a traffic information DB for moving vehicles. Therefore, in this paper, assuming a smart city built on the basis of a camera infrastructure such as intelligent CCTV on the road, a solution for calculating the traffic DB of moving vehicles using Vision Tracking of road CCTV cameras is presented. Simulation and verification of basic performance were conducted and solution can be usefully utilized in related fields as a new intelligent traffic DB calculation solution that reflects the environment of road-mounted CCTV cameras and moving vehicles in a variable smart city road environment. It is expected to be there.

• The Journal of the Institute of Internet, Broadcasting and Communication
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• v.18 no.4
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• pp.43-48
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• 2018

This study was conducted to reproduce digital colour based on the lightness of the background and size of the colour stimulus so that colour can be similarly perceived under different conditions. With the evolution of display technologies, display devices of various sizes can now reproduce more accurate colour and enhanced images, thus affecting the overall quality of display images. This study reproduced digital colour by considering the visual characteristics of the digital media environment. To accomplish this, we developed a colour appearance model which distinguishes the properties of foveal and peripheral vision. The proposed model is based on existing research on the lightness of the background and size of the colour stimulus. Based on experimental results, an analysis of variance was performed in order to develop the colour appearance model. The algorithm and modelling were verified based on the proposed model. In addition, to apply this model to display technologies, a practical colour control system and a method for handling complex input images were developed. Through this research, colour conversion errors which might occur when the input image is converted to fit a specific display size are resolved from the perspective of the human visual system. As a result, more accurate colour can be displayed and enhanced images can be reproduced.