• 제목/요약/키워드: Image Sequence Database

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Differential Protein Expression in EC304 Gastric Cancer Cells Induced by Alphastatin

  • Wang, Xin-Xin;Sun, Rong-Ju;Wu, Meng;Li, Tao;Zhang, Yong;Chen, Lin
    • Asian Pacific Journal of Cancer Prevention
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    • v.13 no.4
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    • pp.1667-1674
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    • 2012
  • Objective: To explore the differential protein expression profile in EC304 gastric cancer cells induced by alphastatin. Methods: Cultured EC304 cells in the exponential phase of growth were randomly divided into alphastatin and control groups. Total proteins were extracted and the two dimensional electrophoresis (2-DE) technique was applied to analyze differences in expression with ImageMaster 2D Platinum 5.0 software. Proteins were identified using the MASCOT database and selected differently expressed proteins were characterised by western blotting and immunofluorescence. Results: $1350{\pm}90$ protein spots were detected by the ImageMaster software in the 2-DE gel images from the control and alphastatin groups. The match rate was about 72-80% for the spectrum profiles, with 29 significantly different protein spots being identified, 10 upregulated, 16 downregulated, two new and one lost. The MASCOT search scores were 64-666 and the peptide matching numbers were 3-27 with sequence coverage of 8-62%. Twenty-three proteins were checked by mass spectrometry, including decrease in Nm23 and profilin-2 isoform b associated with the regulation of actin multimerisation induced by extracellular signals. Conclusion: The proteome in EC304 cells is dramatically altered by alphastatin, which appears to play an important role in modulating cellular activity and anti-angiogenesis by regulating protein expression and signal transduction pathways through Nm23 and profilin-2 isoform b, providing new research directions for anti-angiogenic therapy of gastric cancer.

Automatic Generation of DB Images for Testing Enterprise Systems (전사적 응용시스템 테스트를 위한 DB이미지 생성에 관한 연구)

  • Kwon, Oh-Seung;Hong, Sa-Neung
    • Journal of Intelligence and Information Systems
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    • v.17 no.4
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    • pp.37-58
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    • 2011
  • In general, testing DB applications is much more difficult than testing other types of software. The fact that the DB states as much as the input data influence and determine the procedures and results of program testing is one of the decisive reasons for the difficulties. In order to create and maintain proper DB states for testing, it not only takes a lot of time and efforts, but also requires extensive IT expertise and business knowledge. Despite the difficulties, there are not enough research and tools for the needed help. This article reports the result of research on automatic creation and maintenance of DB states for testing DB applications. As its core, this investigation develops an automation tool which collects relevant information from a variety of sources such as log, schema, tables and messages, combines collected information intelligently, and creates pre- and post-Images of database tables proper for application tests. The proposed procedures and tool are expected to be greatly helpful for overcoming inefficiencies and difficulties in not just unit and integration tests but including regression tests. Practically, the tool and procedures proposed in this research allows developers to improve their productivity by reducing time and effort required for creating and maintaining appropriate DB sates, and enhances the quality of DB applications since they are conducive to a wider variety of test cases and support regression tests. Academically, this research deepens our understanding and introduces new approach to testing enterprise systems by analyzing patterns of SQL usages and defining a grammar to express and process the patterns.

Personalized Exhibition Booth Recommendation Methodology Using Sequential Association Rule (순차 연관 규칙을 이용한 개인화된 전시 부스 추천 방법)

  • Moon, Hyun-Sil;Jung, Min-Kyu;Kim, Jae-Kyeong;Kim, Hyea-Kyeong
    • Journal of Intelligence and Information Systems
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    • v.16 no.4
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    • pp.195-211
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    • 2010
  • An exhibition is defined as market events for specific duration to present exhibitors' main product range to either business or private visitors, and it also plays a key role as effective marketing channels. Especially, as the effect of the opinions of the visitors after the exhibition impacts directly on sales or the image of companies, exhibition organizers must consider various needs of visitors. To meet needs of visitors, ubiquitous technologies have been applied in some exhibitions. However, despite of the development of the ubiquitous technologies, their services cannot always reflect visitors' preferences as they only generate information when visitors request. As a result, they have reached their limit to meet needs of visitors, which consequently might lead them to loss of marketing opportunity. Recommendation systems can be the right type to overcome these limitations. They can recommend the booths to coincide with visitors' preferences, so that they help visitors who are in difficulty for choices in exhibition environment. One of the most successful and widely used technologies for building recommender systems is called Collaborative Filtering. Traditional recommender systems, however, only use neighbors' evaluations or behaviors for a personalized prediction. Therefore, they can not reflect visitors' dynamic preference, and also lack of accuracy in exhibition environment. Although there is much useful information to infer visitors' preference in ubiquitous environment (e.g., visitors' current location, booth visit path, and so on), they use only limited information for recommendation. In this study, we propose a booth recommendation methodology using Sequential Association Rule which considers the sequence of visiting. Recent studies of Sequential Association Rule use the constraints to improve the performance. However, since traditional Sequential Association Rule considers the whole rules to recommendation, they have a scalability problem when they are adapted to a large exhibition scale. To solve this problem, our methodology composes the confidence database before recommendation process. To compose the confidence database, we first search preceding rules which have the frequency above threshold. Next, we compute the confidences of each preceding rules to each booth which is not contained in preceding rules. Therefore, the confidence database has two kinds of information which are preceding rules and their confidence to each booth. In recommendation process, we just generate preceding rules of the target visitors based on the records of the visits, and recommend booths according to the confidence database. Throughout these steps, we expect reduction of time spent on recommendation process. To evaluate proposed methodology, we use real booth visit records which are collected by RFID technology in IT exhibition. Booth visit records also contain the visit sequence of each visitor. We compare the performance of proposed methodology with traditional Collaborative Filtering system. As a result, our proposed methodology generally shows higher performance than traditional Collaborative Filtering. We can also see some features of it in experimental results. First, it shows the highest performance at one booth recommendation. It detects preceding rules with some portions of visitors. Therefore, if there is a visitor who moved with very a different pattern compared to the whole visitors, it cannot give a correct recommendation for him/her even though we increase the number of recommendation. Trained by the whole visitors, it cannot correctly give recommendation to visitors who have a unique path. Second, the performance of general recommendation systems increase as time expands. However, our methodology shows higher performance with limited information like one or two time periods. Therefore, not only can it recommend even if there is not much information of the target visitors' booth visit records, but also it uses only small amount of information in recommendation process. We expect that it can give real?time recommendations in exhibition environment. Overall, our methodology shows higher performance ability than traditional Collaborative Filtering systems, we expect it could be applied in booth recommendation system to satisfy visitors in exhibition environment.

Design of Moving Picture Retrieval System using Scene Change Technique (장면 전환 기법을 이용한 동영상 검색 시스템 설계)

  • Kim, Jang-Hui;Kang, Dae-Seong
    • Journal of the Institute of Electronics Engineers of Korea SP
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    • v.44 no.3
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    • pp.8-15
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    • 2007
  • Recently, it is important to process multimedia data efficiently. Especially, in case of retrieval of multimedia information, technique of user interface and retrieval technique are necessary. This paper proposes a new technique which detects cuts effectively in compressed image information by MPEG. A cut is a turning point of scenes. The cut-detection is the basic work and the first-step for video indexing and retrieval. Existing methods have a weak point that they detect wrong cuts according to change of a screen such as fast motion of an object, movement of a camera and a flash. Because they compare between previous frame and present frame. The proposed technique detects shots at first using DC(Direct Current) coefficient of DCT(Discrete Cosine Transform). The database is composed of these detected shots. Features are extracted by HMMD color model and edge histogram descriptor(EHD) among the MPEG-7 visual descriptors. And detections are performed in sequence by the proposed matching technique. Through this experiments, an improved video segmentation system is implemented that it performs more quickly and precisely than existing techniques have.