• Archives of Pharmacal Research
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• v.10 no.2
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• pp.132-141
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• 1987

New triterpenoid saponins, ilexosides A, D, E, J, K and O have been isolated form the root of Ilex pubescens. Chemical and spectroscopic studies have established their structures as shown in formulae 1, 2, 8, 11, 3, 4 and 5.

• Archives of Pharmacal Research
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• v.10 no.2
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• pp.115-120
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• 1987

Methanol extract of Ilex pubescens roots prolonged bleeding time threefold, and inhibited the generation of malondialdehyde released during platelet aggregation inducted by thrombin. Through several purification procedures, its saponin, named ilexoside, was proved to be responsible for the antithrombotic activities of the plant. Ilexosides A, -D and -J and 24-carboxypomolic acid showed strong inhibitory activities on platelet aggregation induced by thrombin.

• Archives of Pharmacal Research
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• v.10 no.2
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• pp.121-131
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• 1987

Four triterpenoids were isolated from the roots of Ilex pubescens : two new triterpenoids named pubescenolic acid (I) and pubescenic acid (II), and two known triterpenoids ilexolic acid (III) and oleanolic acid (IV). Chemical and spectroscopic studies have established I and II as 20-epipomolic acid and 24-carboxypomolic acid, repectively.

• Archives of Pharmacal Research
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• v.14 no.4
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• pp.352-356
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• 1991

The anti-platelet activity of ilexoside D isolated from the roots of Ilex pubescens Hook. et Arn. was investigated in in vitro and ex vivo models of platelet aggregation induced by ADP, thrombin or collagen in rats. In vitro ilexoside D inhibited more effectively platelet aggregation induced by ADP and thrombin than by collagen as compared with aspirin. Ex vivo ilexoside D also inhibited platelet aggregation induced by ADP and collagen, but not by thrombin, and the inhibitory action of ilexoside D was more effective than that of aspirin. However, in vitro ilexoside D inhibited very poorly the generation of malonyldialdehyde, which is known to be concomitantly released with thromboxane $A_2$ during platelet aggregation. These results suggest that the anti-platelet activity of ilexoside D may not be responsible for prostaglandin synthesis in platelets.