• 수산해양기술연구
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• 제49권1호
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• pp.18-28
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• 2013

It was compared the estimated parameters by the surplus production from three different models, i.e., three types (Schaefer, Gulland, and Schnute) of the traditional surplus production models, a stock production model incorporating covariates (ASPIC) model and a maximum entropy (ME) model. We also evaluated the performance of models in the estimation of their parameters. The maximum sustainable yield (MSY) of small yellow croaker (Pseudosciaena polyactis) in Korean waters ranged from 35,061 metric tons (mt) by Gulland model to 44,844mt by ME model, and fishing effort at MSY ($f_{MSY}$) ranged from 262,188hauls by Schnute model to 355,200hauls by ME model. The lowest root mean square error (RMSE) for small yellow croaker was obtained from the Gulland surplus production model, while the highest RMSE was from Schnute model. However, the highest coefficient of determination ($R^2$) was from the ME model, but the ASPIC model yielded the lowest coefficient. On the other hand, the MSY of Kapenta (Limnothrissa miodon) ranged from 16,880 mt by ASPIC model to 25,373mt by ME model, and $f_{MSY}$, from 94,580hauls by ASPIC model to 225,490hauls by Schnute model. In this case, both the lowest root mean square error (RMSE) and the highest coefficient of determination ($R^2$) were obtained from the ME model, which showed relatively better fits of data to the model, indicating that the ME model is statistically more stable and robust than other models. Moreover, the ME model could provide additional ecologically useful parameters such as, biomass at MSY ($B_{MSY}$), carrying capacity of the population (K), catchability coefficient (q) and the intrinsic rate of population growth (r).

• The Korean Journal of Pain
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• 제10권2호
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• pp.166-169
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• 1997

Background: We have noted a high incidence of vasovagal episodes associated with use of interscalene block(ISB) for shoulder arthroscopy in the sitting position. The purpose of this retrospective study is to alert the possibility of potentially dangerous vasovagal events, describe the characteristics of this problem, and propose therapeutic devices. Methods: Results 62 patients who underwent shoulder arthroscopy in the sitting position with ISB were retrospectively analyzed. Group 1, 10 patients experienced vasovagal events characterized by sudden hypotension and bradycardia. Remaining 52 patients, Group 2, did not experience these symptoms. All patient charts were reviewed for age, sex, side of surgery, premedication, preoperative fluid and intraoperative medications. Perioperative hemodynamic changes were also compared between the two groups. Results: Vasovagal events experienced in 16% of patients(10/62) and occurred $39{\pm}18$ min after sitting position and $22{\pm}18$ min after start of operation. Number of patients who receiving anticholinergics for premedication were significantly lower in Group 1 than Group 2(2/10 vs. 28/52, p<0.05). Conclusions: Bezold-parish reflex is a potential mechanism for sudden hypotension and bradycardia which can occur during shoulder arthroscopy in sitting position. Therefore anticholinergic pretreatment and meticulous monitoring during operation are recommended to prevent B-J reflex.

• 한국식품과학회지
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• 제22권7호
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• pp.833-839
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• 1990

고추가루 저장성에 미치는 질소치환의 영향을 검토하기 위하여 온도 및 포장조건에 따른 고추가루의 품질 변화를 조사하였다. 고추가루 저장 중 capsanthin 및 capsaicin 함량은 온도가 증가함에 따라서 이들 함량의 감소가 증가하였으며, 질소가스 치환포장은 함기포장보다 온도 및 포장조건에 따라서 이들 함량의 감소가 적었다. 고추가루 저장 중 갈변도는 저장온도 및 포장조건에 따라서 상이 하였는데, 온도가 증가할수록 갈변도는 증가하였고 질소가스 치환포장은 함기포장 보다 갈변도가 감소하였다. 그리고 표면색깔 변화 중 저장 10개월 동안 L값과 b값은 거의 변화가 없었으나 a값은 온도가 올라감에 따라 감소하였다. 따라서 고추가루 저장 중 질소가스 치환포장은 함기포장 보다 고추가루의 품질이 우수하였다.

• 한국통신학회논문지
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• 제34권11C호
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• pp.1040-1048
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• 2009

본 논문에서는 인물 영상에 대한 새로운 자동 톤 균형 알고리즘을 제안하였다. 일반적인 백색 균형 (White balance) 알고리즘은 무채색 영역 또는 물리적 반사율을 알고 있는 피사체에 대해 RGB 출력 신호의 비율이 동일하도록 보정하는 방법을 사용한다. 본 논문에서는 사진 속 얼굴색을 기준 조명하에서의 피부색 좌표와 일치 되도록 RGB의 채널 이득을 조절한다. 실험을 위해 카메라 이미지 센서의 전달 특성을 분석하고 기준 조명하에서의 평균 얼굴 색도를 측정하여 카메라 출력 RGB을 계산한다. 마지막으로 임의의 조명하에서 촬영된 인물 영상에 대해 얼굴 부분의 RGB 출력 비율이 기준 얼굴색에 대한 비율이 되도록 R 채널과 B 채널의 이득을 조정하여 색도 보정을 했다. 입력 삼자극치 XYZ는 카메라 전달 행렬에 의해 카레라 출력 RGB로부터 계산된다. 그리고 입력 삼자극치 XYZ는 sRGB 전달 행렬을 이용하여 기준 컬러 공간(sRGB)으로 변환된다. RGB 데이터는 감마 보정 후 디스플레이를 위해 8 bit 데이터로 인코딩되어진다. 알고리즘은 맥베스 컬러 차트 (Macbeth color chart)의 light skin color인 평균 얼굴색과 실제로 측정된 다양한 얼굴색의 평균색에 적용되어졌다.

• Journal of Ginseng Research
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• 제39권4호
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• pp.331-337
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• 2015

Background: The biological actions of various ginseng extracts have been studied for treating obesity and diabetes mellitus. However, few studies have evaluated the effects of fermented Korean Red Ginseng (Panax ginseng Meyer) on metabolic syndrome. The present study evaluated the antiobesity and antidiabetic effects of fermented red ginseng (FRG) on old-aged, obese, leptin-deficient (B6.V-Lepob, "ob/ob") mice. Methods: The animals were divided into three groups and given water containing 0%, 0.5%, and 1.0% FRG for 16 wk. The effect of FRG on ob/ob mice was determined by measuring changes in body weight, levels of blood glucose, serum contents of triglycerides, total cholesterol and free fatty acids, messenger RNA (mRNA) expressions of key factors associated with insulin action, such as insulin receptor (IR), lipoprotein lipase (LPL), glucose transporter 1 and 4 (GLUT1 and GLUT4), peroxisome proliferators-activated receptor gamma ($PPAR-{\gamma}$), and phosphoenolpyruvate carboxykinase (PEPCK) in the liver and in muscle, and histology of the liver and pancreas. Results: FRG-treated mice had decreased body weight and blood glucose levels compared with control ob/ob mice. However, anti-obesity effect of FRG was not evident rather than hypoglycemic effect in old aged ob/ob mice. The hyperlipidemia in control group was attenuated in FRG-treated ob/ob mice. The mRNA expressions of IR, LPL, GLUT1, GLUT4, $PPAR-{\gamma}$, and PEPCK in the liver and in muscle were increased in the FRG-treated groups compared with the control group. Conclusion: These results suggest that FRG may play a vital role in improving insulin sensitivity relative to reducing body weight in old-aged ob/ob mice.

• Journal of Ginseng Research
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• 제39권4호
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• pp.376-383
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• 2015

Background: Panax ginseng has a wide range of biological activities including anti-inflammatory, antioxidant, and immunomodulatory functions. Wild ginseng cambial meristematic cells (CMCs) were obtained from P. ginseng cambium. This study examined the protective mechanism of wild ginseng CMCs against $\small{D}$-galactosamine (GalN)-induced liver injury. GalN, a well-known hepatotoxicant, causes severe hepatocellular inflammatory damage and clinical features similar to those of human viral hepatitis in experimental animals. Methods: Hepatotoxicity was induced in rats using GalN (700 mg/kg, i.p.). Wild ginseng CMCs was administered orally once a day for 2 wks, and then 2 h prior to and 6 h after GalN injection. Results: Wild ginseng CMCs attenuated the increase in serum aminotransferase activity that occurs 24 h after GalN injection. Wild ginseng CMCs also attenuated the GalN-induced increase in serum tumor necrosis factor-${\alpha}$, interleukin-6 level, and hepatic cyclooxygenase-2 protein and mRNA expression. Wild ginseng CMCs augmented the increase in serum interleukin -10 and hepatic heme oxygenase-1 protein and mRNA expression that was induced by GalN, inhibited the increase in the nuclear level of nuclear factor-kappa B, and enhanced the increase in NF-E2-related factor 2. Conclusion: Our findings suggest that wild ginseng CMCs protects liver against GalN-induced inflammation by suppressing proinflammatory mediators and enhancing production of anti-inflammatory mediators.

• Journal of Ginseng Research
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• 제42권3호
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• pp.379-388
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• 2018

Background: Ginsenosides are the main ingredients of Korean Red Ginseng. They have extensively been studied for their beneficial value in neurodegenerative diseases such as Parkinson's disease (PD). However, the multitarget effects of Korean Red Ginseng extract (KRGE) with various components are unclear. Methods: We investigated the multitarget activities of KRGE on neurological dysfunction and neurotoxicity in a 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced mouse model of PD. KRGE (37.5 mg/ kg/day, 75 mg/kg/day, or 150 mg/kg/day, per os (p.o.)) was given daily before or after MPTP intoxication. Results: Pretreatment with 150 mg/kg/day KRGE produced the greatest positive effect on motor dysfunction as assessed using rotarod, pole, and nesting tests, and on the survival rate. KRGE displayed a wide therapeutic time window. These effects were related to reductions in the loss of tyrosine hydroxylase-immunoreactive dopaminergic neurons, apoptosis, microglial activation, and activation of inflammatory factors in the substantia nigra pars compacta and/or striatum after MPTP intoxication. In addition, pretreatment with KRGE activated the nuclear factor erythroid 2-related factor 2 pathways and inhibited phosphorylation of the mitogen-activated protein kinases and nuclear factor-kappa B signaling pathways, as well as blocked the alteration of blood-brain barrier integrity. Conclusion: These results suggest that KRGE may effectively reduce MPTP-induced neurotoxicity with a wide therapeutic time window through multitarget effects including antiapoptosis, antiinflammation, antioxidant, and maintenance of blood-brain barrier integrity. KRGE has potential as a multitarget drug or functional food for safe preventive and therapeutic strategies for PD.

• Bulletin of the Korean Chemical Society
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• 제33권4호
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• pp.1253-1258
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• 2012

Two phenolic allopyranosides and two phenolic amide allopyranosides, along with eight known phenolic compounds, including cimicifugic acids, shomaside B, fukiic acid, isoferulic acid, and piscidic acid, were isolated from the n-butanolic extract of rhizomes of Cimicifuga heracleifolia. On-line spectroscopic data for UV, NMR, and MS from a combination of LC-NMR and LC-MS techniques directly and rapidly provided sufficient structural information to identify and confirm all the structures of major phenolic compounds in the extract, in addition to their HPLC profiles. This combined analytic information was then used as a dereplication tool for structure-guided screening in order to isolate unknown phenolic compounds in the extract. Successive fractionation and purification using semi-preparative HPLC acquired four unknown allopyranosides, and their structures were identified as cis-ferulic acid 4-O-${\beta}$-D-allopyranoside, trans-ferulic acid 4-O-${\beta}$-D-allopyranoside, trans-feruloyltyramine 4-O-${\beta}$-D-allopyranoside, and trans-feruloyl-(3-O-methyl)dopamine 4-O-${\beta}$-D-allopyranoside, based on a subsequent spectroscopic interpretation.

• Bulletin of the Korean Chemical Society
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• 제25권11호
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• pp.1661-1666
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• 2004

The reaction of titanium tetraisopropoxide with 2 equiv of N,N-diethyl acetoacetamide affords Ti($O^iPr)_2(CH_3COCHCONEt_2)_2$ (1) as colorless crystals in 80% yield. Compound 1 is characterized by spectroscopic (Mass and $^1H/^{13}C$ NMR) and microanalytical data. Molecular structure of 1 has been determined by a single crystal X-ray diffraction study, which reveals that it is a monomeric, cis-diisopropoxide and contains a six coordinate Ti(IV) atom with a cis($CONEt_2$), trans($COCH_3$) configuration (1a) in a distorted octahedral environment. Variable-temperature $^1H$ NMR spectra of 1 indicate that it exists as an equilibrium mixture of cis, trans (1a) and cis, cis (1b) isomers in a 0.57 : 0.43 ratio at -20$^{\circ}C$ in toluene-$d_8$ solution. Thermal properties of 1 as a MOCVD precursor for titanium dioxide films have been evaluated by thermal gravimetric analysis and vapor pressure measurement. Thin films of pure anatase titanium dioxide (after annealing above 500$^{\circ}C$ under oxygen) have been grown on Si(100) with precursor 1 in the substrate temperature range of 350- 500$^{\circ}$ using a bubbler-based MOCVD method.

• Animal cells and systems
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• 제15권1호
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• pp.29-36
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• 2011

Insect lysozymes are basic, cationic proteins synthesized in fat body and hemocytes in response to bacterial infections and depolymerize the bacterial cell wall. The c-type lysozyme of the insect Spodoptera litura (SLLyz) is a single polypeptide chain of 121 residues with four disulfide bridges and 17 rare codons and is approximately 15 kDa. The full-length SLLyz cDNA is 1039 bp long with a poly(A) tail, and contains an open reading frame of 426 bp long (including the termination codon), flanked by a 54 bp long 5' UTR and a 559 bp long 3' UTR. As a host for the production of high-level recombinant proteins, E. coli is used most commonly because of its low cost and short generation time. However, the soluble expression of heterologous proteins in E. coli is not trivial, especially for disulfide-bonded proteins. In order to prevent inclusion body formation, GST was selected as a fusion partner to enhance the solubility of recombinant protein, and fused to the amplified products encoding mature SLLyz. The expression vector pGEX-4T-1/rSLLyz was then transformed into E. coli BL21(DE3)pLysS for soluble expression of rSLLyz, and the soluble fusion protein was purified successfully. Inhibition zone assay demonstrated that rSLLyz showed antibacterial activity against B. megaterium. These results demonstrate that the GST fusion expression system in E. coli described in this study is efficient and inexpensive in producing a disulfide-bonded rSLLyz in soluble, active form, and suggest that the insect lysozyme is an interesting system for future structural and functional studies.