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Verification the Systems Thinking Factor Structure and Comparison of Systems Thinking Based on Preferred Subjects about Elementary School Students' (초등학생의 시스템 사고 요인 구조 검증과 선호 과목에 따른 시스템 사고 비교)

  • Lee, Hyonyong;Jeon, Jaedon;Lee, Hyundong
    • Journal of The Korean Association For Science Education
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    • v.39 no.2
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    • pp.161-171
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    • 2019
  • The purposes of this study are: 1) to verify the systems thinking factor structure of elementary school students and 2) to compare systems thinking according to their preferred subjects in order to get implications for following research. For the study, pre-tests analyze data from 732 elementary school students using the STMI (Systems Thinking Measuring Instrument) developed by Lee et al. (2013). And exploratory factor analysis was conducted to identify the factor structure of the students. Based on the results of the pre-test, the expert group council revised the STMI so that elementary school students could respond to the 5-factor structure that STMI intended. In the post-test, 503 data were analyzed by modified STMI and exploratory factor analysis was performed. The results of the study are as follows: First, in the pre-test, elementary school students responded to the STMI with a test paper consisting of two factors (personal internal factors and personal external factors). The total reliability of the instrument was .932 and the reliability of each factor was analyzed as .857 and .894. Second, for modified STMI, elementary school students responded a 4-factor instrument. Team learning, Shared Vision, and Personal Mastery were derived independent factors, and mental model and systems analysis were derived 1-factor. The total reliability of the instrument was .886 and the reliability of each factor was analyzed as .686 to .864. Finally, a comparison of systems thinking according to preferred subjects showed a significant difference between students who selected science (engineering) group and art (music and physical education). In conclusion, it was confirmed that statistically meaningful results could be obtained using STMI modified by term and sentence structure appropriate for elementary school students, and it is a necessary to study the relation of systems thinking with various student variables such as the preferred subjects.

Relationship between porcine miR-20a and its putative target low-density lipoprotein receptor based on dual luciferase reporter gene assays

  • Ding, Yueyun;Zhu, Shujiao;Wu, Chaodong;Qian, Li;Li, DengTao;Wang, Li;Wan, Yuanlang;Zhang, Wei;Yang, Min;Ding, Jian;Wu, Xudong;Zhang, Xiaodong;Gao, Yafei;Yin, Zongjun
    • Asian-Australasian Journal of Animal Sciences
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    • v.32 no.7
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    • pp.922-929
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    • 2019
  • Objective: Mutations in low-density lipoprotein receptor (LDLR), which encodes a critical protein for cholesterol homeostasis and lipid metabolism in mammals, are involved in cardiometabolic diseases, such as familial hypercholesterolemia in pigs. Whereas microRNAs (miRNAs) can control LDLR regulation, their involvement in circulating cholesterol and lipid levels with respect to cardiometabolic diseases in pigs is unclear. We aimed to identify and analyze LDLR as a potential target gene of SSC-miR-20a. Methods: Bioinformatic analysis predicted that porcine LDLR is a target of SSC-miR-20a. Wild-type and mutant LDLR 3'-untranslated region (UTR) fragments were generated by polymerase chain reaction (PCR) and cloned into the pGL3-Control vector to construct pGL3 Control LDLR wild-3'-UTR and pGL3 Control LDLR mutant-3'-UTR recombinant plasmids, respectively. An miR-20a expression plasmid was constructed by inserting the porcine premiR-20a-coding sequence between the HindIII and BamHI sites in pMR-mCherry, and constructs were confirmed by sequencing. HEK293T cells were co-transfected with the miR-20a expression or pMR-mCherry control plasmids and constructs harboring the corresponding 3'-UTR, and relative luciferase activity was determined. The relative expression levels of miR-20a and LDLR mRNA and their correlation in terms of expression levels in porcine liver tissue were analyzed using reverse-transcription quantitative PCR. Results: Gel electrophoresis and sequencing showed that target gene fragments were successfully cloned, and the three recombinant vectors were successfully constructed. Compared to pMR-mCherry, the miR-20a expression vector significantly inhibited wild-type LDLR3'-UTR-driven (p<0.01), but not mutant LDLR-3'-UTR-driven (p>0.05), luciferase reporter activity. Further, miR-20a and LDLR were expressed at relatively high levels in porcine liver tissues. Pearson correlation analysis revealed that porcine liver miR-20a and LDLR levels were significantly negatively correlated (r = -0.656, p<0.05). Conclusion: LDLR is a potential target of miR-20a, which might directly bind the LDLR 3'-UTR to post-transcriptionally inhibit expression. These results have implications in understanding the pathogenesis and progression of porcine cardiovascular diseases.

Improvement of Nitrification Efficiency by Activated Nitrifying Bacteria Injection at Low Temperature (활성화된 질산화균 주입에 의한 저온 질산화효율 향상)

  • Lim, Dongil;Kim, Younghee
    • Journal of the Korean Society of Urban Environment
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    • v.18 no.4
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    • pp.473-483
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    • 2018
  • In this study, we have developed a lab scale bioreactor to identify the characteristics of nitrification reaction according to operation condition (temperature, inhibitor (as Cl), activated nitrifying bacteria (ANB). etc) to improve nitrification efficiency at low temperature. Recovery rate of nitrification took about 4 days to reach the normal level by injected ANB after inhibition shock of CI injection at $20^{\circ}C$, when measured the concentration of $NO_2{^-}-N+NO_3{^-}-N$ in the effluent. In the case of $10^{\circ}C$, recovery of nitrification rate took about 4 days to reach the level of half to the normal level and 7 days for complete recovery which took 3 days more than those at $20^{\circ}C$. At $10^{\circ}C$ considering the winter season, the specific nitrification rate(SNR) of the from 1 day to 6 days after injected ANB according to its operation condition increased from 0.029 to 0.767 mgN/gSS/hr. The simulated SNR for the 8th day after the injected ANB at $10^{\circ}C$ was 0.840, 3.625 mgN/gSS/hr, respectively as linear function and exponential function, expecting to exceed level of 2.592 mgN/gSS/hr at normal condition. It was confirmed that injection of ANB during low temperature operation has many effects for improving nitrification efficiency through this study. In future studies, if further studies are carried out the determination of ANB injection and the design of efficient ANB reactor considering the changes of operating characteristics by site, it will contribute to the improvement of nitrification efficiency in winter season.

A Study on the Characteristics of PM1.0 Chemical Components Using a Real-time Aerosol Mass Spectrometer (실시간 에어로졸 질량분석기를 이용한 PM1.0의 화학적성분의 특성에 관한 연구)

  • Park, Jinsoo;Choi, Jinsoo;Kim, Hyunjae;Oh, Jun;Sung, Minyoung;Ahn, Joonyoung;Lee, Sangbo;Kim, Jeongho
    • Journal of the Korean Society of Urban Environment
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    • v.18 no.4
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    • pp.485-494
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    • 2018
  • This study aims to identify the characteristics of oxidation and chemical composition of PM in winter season, 2017 at Incheon area. The mean concentration of air pollutants were $46{\pm}22{\mu}g/m^3-PM_{10}$, $29{\pm}18{\mu}g/m^3/-PM_{2.5}$, $5{\pm}3ppb-SO_2$, $0.56{\pm}0.24ppm-CO$, $21{\pm}13ppb-O_3$ and $28{\pm}17ppb-NO_2$, respectively. The dominant ion of the $PM_{1.0}$ chemical component were organic with $3.2{\mu}g/m^3$ and nitrate with $1.9{\mu}g/m^3$. The day and night variation of the $PM_{1.0}$ chemical components was higher in nighttime than those of daytime. The averaged nitrate oxidation rate (SOR) was 0.06 and sulfate oxidation rate was 0.11 during the field campaign. In the high mass loading period, nitrate oxidation rate (NOR) was up to 0.6 and also the nitrate in $PM_{1.0}$ was increased. The averaged ratio of $NO_x/SO_2$ was 8.7 and nitrate/sulfate was 3.1, respectively. In this results, the nitrate component in $PM_{1.0}$ was influenced by NOx from the stationary source as power plant and the mobile source around the measurement site.

Identification and Chromosomal Reshuffling Patterns of Soybean Cultivars Bred in Gangwon-do using 202 InDel Markers Specific to Variation Blocks (변이영역 특이 202개 InDel 마커를 이용한 강원도 육성 콩 품종의 판별 및 염색체 재조합 양상 구명)

  • Sohn, Hwang-Bae;Song, Yun-Ho;Kim, Su-Jeong;Hong, Su-Young;Kim, Ki-Deog;Koo, Bon-Cheol;Kim, Yul-Ho
    • Korean Journal of Breeding Science
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    • v.50 no.4
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    • pp.396-405
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    • 2018
  • The areas of soybean (Glycine max (L.) Merrill) cultivation in Gangwon-do have increased due to the growing demand for well-being foods. The soybean barcode system is a useful tool for cultivar identification and diversity analysis, which could be used in the seed production system for soybean cultivars. We genotyped cultivars using 202 insertion and deletion (InDel) markers specific to dense variation blocks (dVBs), and examined their ability to identify cultivars and analyze diversity by comparison to the database in the soybean barcode system. The genetic homology of "Cheonga," "Gichan," "Daewang," "Haesal," and "Gangil" to the 147 accessions was lower than 81.2%, demonstrating that these barcodes have potentiality in cultivar identification. Diversity analysis of one hundred and fifty-three soybean cultivars revealed four subgroups and one admixture (major allele frequency <0.6). Among the accessions, "Heugcheong," "Hoban," and "Cheonga" were included in subgroup 1 and "Gichan," "Daewang," "Haesal," and "Gangil" in the admixture. The genetic regions of subgroups 3 and 4 in the admixture were reshuffled for early maturity and environmental tolerance, respectively, suggesting that soybean accessions with new dVB types should be developed to improve the value of soybean products to the end user. These results indicated that the two-dimensional barcodes of soybean cultivars enable not only genetic identification, but also management of genetic resources through diversity analysis.

The Influence of Moral Distress and Moral Sensitivity on Moral Courage in Nursing Students (간호대학생의 도덕적 용기에 대한 도덕적 고뇌와 도덕적 민감성의 관계)

  • YUN, Hye-Young;KIM, Sun-Ki;JANG, Hyo-Eun;HWANG, Sin-Woo;KIM, Sang-Hee
    • Korean Journal of Medical Ethics
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    • v.21 no.4
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    • pp.360-376
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    • 2018
  • Nursing students experience ethical conflicts that lead to moral distress and moral sensitivity in clinical practice. Most nursing students have some difficulty in speaking up when faced with morally challenging situations. Hence, increasing moral courage of these students is important to improve the quality of practice, and carry out nursing responsibilities. However, research on the moral distress, moral sensitivity, and moral courage of nursing students has not been reported in South Korea. The purposes of this study were to (a) identify the levels of moral distress, moral sensitivity, and moral courage of nursing students and (b) examine the influence of moral distress and moral sensitivity on moral courage. Data were collected through a survey using self-reported questionnaires sent to senior nursing students at two nursing colleges in Seoul and Gyeonggido. A total of 138 senior nursing students participated in the survey. The data were analyzed using the IBM SPSS Statistics 23 program by Pearson's correlation coefficients and multiple regression analysis. The mean scores of the moral distress thermometer, moral distress, moral sensitivity, and moral courage were $3.53{\pm}2.18$, $57.33{\pm}43.35$, $134.98{\pm}13.98$, and $56.33{\pm}12.75$, respectively. The significant factors influencing moral courage were the moral distress thermometer and patient-centered nursing, which was a subcomponent of moral sensitivity. The explanatory power of the model was 5%. This study confirms that nursing students, like nurses, experience moral distress. It is therefore important to create organizational environments that support the moral courage of nursing students.

A Study on Survey of Improvement of Non Face to Face Education focused on Professor of Disaster Management Field in COVID-19 (코로나19 상황에서 재난분야 교수자를 대상으로 한 비대면 교육의 개선에 관한 조사연구)

  • Park, Jin Chan;Beck, Min Ho
    • Journal of the Society of Disaster Information
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    • v.17 no.3
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    • pp.640-654
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    • 2021
  • Purpose: Normal education operation was difficult in the national disaster situation of Coronavirus Infection-19. Non-face-to-face education can be an alternative to face to face education, but it is not easy to provide the same level of education. In this study, the professor of disaster management field will identify problems that can occur in the overall operation and progress of non-face-to-face education and seek ways to improve non-face-to-face education. Method: Non-face-to-face real-time education was largely categorized into pre-class, in-class, post-class, and evaluation, and case studies were conducted through the professor's case studies. Result&Conclusion: The results of the survey are as follows: First, pre-class, it was worth considering providing a non-face-to-face educational place for professors, and the need for prior education on non-face-to-face educational equipment and systems was required. In addition, it seems necessary to make sure that education is operated smoothly by giving enough notice on classes and to make efforts to develop non-face-to-face education programs for practical class. Second, communication between professor and learner, and among learners can be an important factor in non-face-to-face mid classes. To this end, it is necessary to actively utilize debate-type classes to lead learners to participate in education and enhance the educational effect through constant interaction. Third, non-face-to-face post classes, policies on the protection of privacy due to video records should be prepared to protect the privacy of professors in advance, and copyright infringement on educational materials should also be considered. In addition, it is necessary to devise various methods for fair and objective evaluation. According to the results of the interview, in the contents, which are components of non-face-to-face education, non-face-to-face education requires detailed plans on the number of students, contents, and curriculum suitable for non-face-to-face education from the design of the education. In the system, it is necessary to give the professor enough time to fully learn and familiarize with the function of the program through pre-education on the program before the professor gives non-face-to-face classes, and to operate the helpdesk, which can thoroughly check the pre-examination before non-face-to-face education and quickly resolve the problem in case of a problem.

A Status Analysis for the Standards on Permission of Altering Cultural Heritage's Current State Focusing on the Results of Handling Application Cases on Permission of State-Designated Cultural Heritage (Historic Site) for the Last Five Years (2015~2019) (문화재 현상변경 인·허가 검토기준 마련을 위한 실태분석 연구 - 최근 5년(2015~2019)간 국가지정문화재(사적)의 허가신청 안건 처리결과를 중심으로 -)

  • CHO, Hongseok;SUH, Hyunjung;CHOI, Jisu
    • Korean Journal of Heritage: History & Science
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    • v.54 no.3
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    • pp.24-51
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    • 2021
  • Since June 2006, there have been active efforts to systematize the permission system including the amendment of [Cultural Heritage Protection Act]. Cultural Heritage Administration prepared standards on reviewing each type of cultural heritages(CH) in 2015, promoted a project on the modification of permission standards and showed remarkable performances in quantitative aspects. But as there has been little change for the cases applied for permission, additional studies on policy are required to improve the management efficiency and reduce the citizens'inconvenience. In response, this study aims to identify the actual management status on the current state alteration permission system, and establish practically utilizable reference materials at permission review. While historic sites(HS) constitute a relatively small proportion in state-designated CHs, they are subject to the designation of permission standards. Also, with their location in the downtown area, the application rate is high (51.4%) and the results are commonly utilizable to other types of CH. We constructed a DB based on the minutes of Cultural Heritage Committee(CHC) on HS and categorized similar features in permission handling results. The result of the analysis is as follows. Out of a total of 5,243 cases for permission applied for HS, 1,734 cases of cultural heritage areas(CHA) and 3,509 cases of historic and cultural environment preservation areas(HCEPA) have been applied. CHA has a great proportion of the applications for events and festivals, which are highly related to CHs or representing the local area. There is a high permission rate on applications for the purpose of public service by local governments. Meanwhile, HCEPA has a high proportion of applying for the installation and extension of buildings and facilities at the private level. Thus, negative decisions were made for tall buildings, massed facilities, or suspected scattering of similar acts. Our actual condition analysis has identified a total of 78 types of harmful acts which may influence the preservation of CHs. 31 types in CHA and 37 types in HCEPA are categorized. Especially, 10 common types of permission have been confirmed in both sectors. As a result, it is expected to secure consistency in the permission administration, enhance the management efficiency and improve the public's satisfaction over the regulatory administration by providing practically utilizable reference materials for altering the current state of CH and for decision making on the part of CHC.

Demonstration of Disaster Information and Evacuation Support Model for the Safety Vulnerable Groups (안전취약계층을 위한 재난정보 및 대피지원 모델 실증)

  • Son, Min Ho;Kweon, Il Ryong;Jung, Tae Ho;Lee, Han Jun
    • Journal of the Society of Disaster Information
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    • v.17 no.3
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    • pp.465-486
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    • 2021
  • Purpose: Since most disaster information systems are centered on non-disabled people, the reality is that there is a lack of disaster information delivery systems for the vulnerable, such as the disabled, the elderly, and children, who are relatively vulnerable to disasters. The purpose of the service is to improve the safety of the disabled and the elderly by eliminating blind spots of informatization and establishing customized disaster information services to respond to disasters through IoT-based integrated control technology. Method: The model at the core of this study is the disaster alert propagation model and evacuation support model, and it shall be developed by reflecting the behavioral characteristics of the disabled and the elderly in the event of a disaster. The disaster alert propagation model spreads disaster situations collected using IoT technology, and the evacuation support model uses geomagnetic field-based measuring technology to identify the user's indoor location and help the disabled and the elderly evacuate safely. Results: Demonstration model demonstration resulted in an efficient qualitative evaluation of indoor location accuracy, such as the suitability of evacuation route guidance and satisfaction of services from the user's perspective. Conclusion: Disaster information and evacuation support services were established for the safety vulnerable groups of mobile app for model verification. The disaster situation was demonstrated through experts in the related fields and the disabled by limiting it to the fire situation. It was evaluated as "satisfaction" in the adequacy of disaster information delivery and evacuation support, and its functional satisfaction and user UI were evaluated as "normal" due to the nature of the pilot model. Through this, the disaster information and evacuation support services presented in this study were evaluated to support the safety vulnerable groups to a faster disaster evacuation without missing the golden time of disaster evacuation.

Comparison of Plant's Growth in Wall Greening Depending on Orientations (방위에 따른 벽면녹화식물의 생육 비교)

  • Kim, Da-Yoon;Cho, Yong-Hyeon;Son, In-Ki;Kim, Yoon-Ho
    • Journal of the Korean Institute of Landscape Architecture
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    • v.49 no.5
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    • pp.71-78
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    • 2021
  • Green areas and the area of available horizontal surfaces are gradually decreasing due to the overcrowding of buildings. It is adversely affecting the urban climate and ecosystem. However, the recognition of the importance of green areas is gradually increasing. As a result, the importance of wall greening using vertical surfaces is growing. However, despite the fact that domestic wall greening guidelines and institutions related to orientations restrict planting. there was no study to determine whether there were actual differences in plant growth due to orientations. Therefore, this study compared and analyzed the plant growth characteristics by orientations to apply actual wall greening to cities. The experiment was conducted from May to September 2020. First of all, three octave walls were constructed to measure the temperature, the illumination, and the length of the plants once a week. The plants included Parthenocissus tricuspidata, Hedera rhombea, and Euonymus radicans cv. Aueonmarinata Rehd plants. As a result of the study, Parthenocissus tricuspidata was prolific in the north, and Hedera rhombea, and Euonymus radicans cv. Aueonmarinata Rehd plants were prolific in the south. All three types of plants were prolific in June-July, and the Parthenocissus tricuspidata was prolific in grass-growing, and in August, all the walls were 100% covered. Hedera rhombea had the lowest rate of herbaceous growth, and the vertical coverate was also lower at an average of 45%, but among the three plants, the sheath of the horizontal surface coverate was the highest. Euonymus radicans cv. Aueonmarinata Rehd was low in the speed of herbaceous growth, and finally, the walls were 100% covered except for the north and northwest directions. It was found that not all plants used for wall greening show the same growth, and the difference in growth varies more depending on plants than the effect of orientations. Therefore, it is better to identify the characteristics of plant growth and plant suitable plants for each directions.