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Distribution and diversity of rhizosphere bacteria of mixed halophytes vegetation native to the Goraebul sand dune, Korea : Approaches to coastal dune conservation (한반도 고래불 해안사구에 자생하는 혼합 염생식물군락 근권세균의 분포 및 다양성 고찰 : 해안사구 보전을 위한 접근)

  • Jong Myong Park;Ji Won Hong;Ki-Eun Lee;Jong-Guk Kim;Young-Hyun You
    • Journal of Marine Bioscience and Biotechnology
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    • v.16 no.1
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    • pp.1-18
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    • 2024
  • Coastal dunes must be conserved. Their native halophytes support coastal geography while their symbiotic microorganisms help vegetation thrive. The Goraebul coast has the largest, well-conserved dune system on the East Sea of the Korean Peninsula due to a climax mixed halophyte (C. soldanella, C. kobomugi, and E. mollis) vegetation support. This study identified rhizobacteria and their diversity in mixed halophyte communities unique to Goraebul. Five phyla, 12 genera, and 21 species were identified based on 16S rDNA sequences from 65 isolates. The phylum Bacillota, class Bacillota, order Bacillales, and family Bacillaceae were identified, with Bacillus as the dominant genus (46.15%). The richness and Shannon's diversity were higher at the species than at the genus level due to the dominance of Bacillus; however, various Bacillus species (7) were identified. Therefore, the climax mixed vegetation adapted to the Goraebul coast may exert natural selection pressure in favor of the common characteristics of Bacillus. However, despite this advantage, the Shannon equitability (0.86), Simpson (0.08), and Shannon diversity (2.79) indexes indicate a stable rhizosphere cluster and the climax mixed vegetation is affected by symbiotic relationships between healthy rhizosphere microbiota.

Peatland restoration research: a global overview with insights from Indonesia

  • Kushartati Budiningsih;Prakoso Bhairawa Putera;Ari Nurlia;Nur Arifatul Ulya;Fitri Nurfatriani;Mimi Salminah;Dhany Yuniati;Asmanah Widarti
    • Journal of Ecology and Environment
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    • v.48 no.3
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    • pp.263-276
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    • 2024
  • Background: Repeated and severe fires have led to a large investment in research directed towards recapturing the natural values of Indonesia's peatland forest resources. The aim of this study was to identify the patterns and trends in research on peatland restoration-related literature available on the Scopus database. Methods in this paper a bibliometric methodology, the Scopus database and VOSviewer were used explore the trends in the published peatland restoration literature in the period 1994-2021; the leading journals and most influential authors, affiliations, countries, documents and research themes were identified. Results: Three hundred and seventeen documents including 266 journal articles were identified. The leading journals based on numbers of articles published and citations were Restoration Ecology and Ecological Engineering. Authors affiliated to institutions in Canada and the United Kingdom were the most influential. Indonesia was the third most influential based on numbers of documents. The most influential article was "The underappreciated potential of peatlands in global climate change mitigation strategies" by Liefield J in Nature Communications with an annual average citation rate of 66/year. A keyword co-occurrence network identified nine main themes in peat restoration research. Conclusions: The findings of the study are used to outline the types of research in peat restoration now required to meet the outstanding and unmet challenges confronted in Indonesia. Three significant challenges have been identified: (1) anthropogenic, those that encompass issues related to community acceptance and participation in peatland restoration, (2) ecological, those associated with severely degraded peatlands, and (3) economic, the absence of secure funding to cover substantial costs.

Aroma Components of Traditional Korean Soy Sauce and Soybean Paste Fermented with the Same Meju

  • Seo, Jae-Soon;Chang, Ho-Geun;Ji, Won-Dae;Lee, Eun-Ju;MYEONG-RAK-CHOI;HAENG-JA-KIM
    • Journal of Microbiology and Biotechnology
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    • v.6 no.4
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    • pp.278-285
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    • 1996
  • We identified volatile components of traditional Korean soy sauce and soybean paste which had been manufactured with the same traditional Meju with a view to improving the quality of traditional Korean soy sauce and soybean paste. All of the volatile components were extracted by simultaneous steam distillation-extraction (SDE) apparatus. To obtain more detailed information, whole volatile components were separated into fractions. The volatile components of the whole and of each fraction were identified by GC-mass and Kovat's retention index. Sixty two and eighty six components were identified in traditional Korean soy sauce and soybean paste, respectively. Many aroma components of traditional Korean soy sauce differ from those of traditional Korean soybean paste. It was confirmed that many aroma components of traditional Korean soy sauce and soybean paste are completely different from those of Japanese fermented soy sauce (Shoyu) and soybean paste (Miso).

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Highly Polymorphic Bovine Leptin Gene

  • Yoon, D.H.;Cho, B.H.;Park, B.L.;Choi, Y.H.;Cheong, H.S.;Lee, H.K.;Chung, E.R.;Cheong, I.C.;Shin, H.D.
    • Asian-Australasian Journal of Animal Sciences
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    • v.18 no.11
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    • pp.1548-1551
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    • 2005
  • The leptin, an anti-obesity protein, is a hormone protein expressed and secreted mainly from adipocyte tissue, and involved in regulation of body weight, food intake and energy metabolism. In an effort to discover polymorphism(s) in genes whose variant(s) might be implicated in phenotypic traits of growth, we have sequenced exons and their boundaries of leptin gene including 1,000 bp upstream of promoter region with twenty-four unrelated Korean cattle. Fifty-seven sequence variants were identified: fourteen in 5' flanking region, twenty-seven in introns, eight in exons, and eight in 3' flanking region. By pair-wise linkage analysis among polymorphisms, ten sets of SNPs were in absolute linkage disequilibrium (LD) (|D'| = 1 and $r^2$ = 1). Among variants identified, thirty-six SNPs were newly identified, and twenty-one SNPs, which were reported in other breeds, were also confirmed in Korean cattle. The allele frequencies of variants were quite different among breeds. The information from SNPs of bovine leptin gene could be useful for further genetic studies of this gene.

Symptom Clusters in Patients with Breast Cancer (유방암 환자의 증상 클러스터)

  • Kim, Soo-Hyun;Lee, Ran;Lee, Keon-Suk
    • Korean Journal of Adult Nursing
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    • v.21 no.6
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    • pp.705-717
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    • 2009
  • Purpose: The purpose of this study was to identify symptom clusters in patients with breast cancer and to investigate the associations among them with functional status and quality of life (QOL). Methods: A convenient sample of 303 patients was recruited from an oncology-specialized hospital. Results: Two distinct clusters were identified: A gastrointestinal- fatigue cluster and a pain cluster. Each cluster significantly influenced functional status and QOL. Based on these two clusters, we identified subgroups of symptom clusters using K-means cluster analysis. Three relatively distinct patient subgroups were identified in each cluster: mild, moderate, and severe group. Disease-related factors (i.e., stage, metastasis, type of surgery, current chemotherapy, and anti-hormone therapy) were associated with these subgroups of symptom clusters. There were significant differences in functional status and QOL among the three subgroups. The subgroup of patients who reported high levels of symptom clusters reported poorer functional status and QOL. Conclusion: Clinicians can anticipate that breast cancer patients with advanced stage, metastasis, and who receive mastectomy, and chemotherapy will have more intense gastrointestinal-fatigue or pain symptoms. In order to enhance functional status and QOL for patients with breast cancer, collective management for symptoms in a cluster may be beneficial.

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Identification of modal damping ratios of structures with closely spaced modal frequencies

  • Chen, J.;Xu, Y.L.
    • Structural Engineering and Mechanics
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    • v.14 no.4
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    • pp.417-434
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    • 2002
  • This paper explores the possibility of using a combination of the empirical mode decomposition (EMD) and the Hilbert transform (HT), termed the Hilbert-Huang transform (HHT) method, to identify the modal damping ratios of the structure with closely spaced modal frequencies. The principle of the HHT method and the procedure of using the HHT method for modal damping ratio identification are briefly introduced first. The dynamic response of a two-degrees-of-freedom (2DOF) system under an impact load is then computed for a wide range of dynamic properties from well-separated modal frequencies to very closely spaced modal frequencies. The natural frequencies and modal damping ratios identified by the HHT method are compared with the theoretical values and those identified using the fast Fourier transform (FFT) method. The results show that the HHT method is superior to the FFT method in the identification of modal damping ratios of the structure with closely spaced modes of vibration. Finally, a 36-storey shear building with a 4-storey light appendage, having closely spaced modal frequencies and subjected to an ambient ground motion, is analyzed. The modal damping ratios identified by the HHT method in conjunction with the random decrement technique (RDT) are much better than those obtained by the FFT method. The HHT method performing in the frequency-time domain seems to be a promising tool for system identification of civil engineering structures.

TRADITIONAL PIG FARMING IN THE SOUTH PACIFIC: PROBLEMS AND OPPORTUNITIES FOR INCREASING PRODUCTIVITY

  • Ochetim, S.
    • Asian-Australasian Journal of Animal Sciences
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    • v.6 no.3
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    • pp.347-360
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    • 1993
  • The project was undertaken to provide information on the present system of traditional pig farming in the South Pacific region, to identify the problems currently limiting productivity of such pigs and to offer practical strategies which could be used for increasing productivity of the animals. The problems were identified by surveying some 220 subsistence pig farms in eleven island countries in the South Pacific region using a prepared questionnaire. The units were found to be generally small, consisting of about 2-4 sows per herd. The productivity of the units as assessed in terms of sow reproductive efficiency was rather low, being only about 7.5. Feed, housing, breeding, disease, marketing, lack of capital, technical know-how and existing social traditions were identified as current constraints. Based on three of the most limiting factors identified namely feed, housing and breeding, strategies for improvement were developed on the basis of better and more effective use of locally available feed resources, better housing and genetic improvement through crossbreeding programmes. These improvement strategies were tested as a package model on some ten farms in two of the island countries. The results of these on-farm trials indicated that using the improvement strategies increased sow reproductive efficiency by approximately 60 percent, to nearly 12. The significance of these findings in the overall management of traditionally raised pigs in the South Pacific region is discussed.

Identification of Predominant Bacteria and Fungi in the Industry Treating Soluble Metal Working Fluids (금속가공유 취급 업종에서 우점하는 세균 및 진균의 정성평가)

  • Park, Hae Dong;Park, Dongjin;Park, Hyunhee
    • Journal of Korean Society of Occupational and Environmental Hygiene
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    • v.24 no.4
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    • pp.416-424
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    • 2014
  • Objectives: The objective of this study is to analyze the predominant microorganisms in the industry treating MWFs(Metal working fluids). Methods: The bacteria and fungi were collected by agar plate impaction and bulk MWFs in storage tank at 54 sites in 9 shops in South Korea. The dominant bacteria and fungi isolated from agar media were identified by fatty acid analysis and morphological analysis, respectively. Results: Totally 111 dominant bacteria were identified in the process, outdoor, and bulk MWFs. The predominant bacterial genus was Micrococcus and Bacillus in the process and outdoor, Pseudomonas in bulk MWF. Among the identified 119 strains of fungi, Cladosporium and Penicillium genus were dominated. The ratios of bacteria designated biosafety level 2 and 1 were 30% and 21%, respectively. Conclusions: This study has investigated the dominant microorganisms in soluble MWF using industry. And it was useful that the qualitative evaluation method along with quantitative analysis for better understanding of the biological factors in the work environment.

Molecular Epidemiology of Clinical Cryptococcus neoformans Isolates in Seoul, Korea

  • Park, So Hae;Kim, Mina;Joo, Sei Ick;Hwang, Soo Myung
    • Mycobiology
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    • v.42 no.1
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    • pp.73-78
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    • 2014
  • Cryptococcal infection is primarily caused by two species, Cryptococcus neoformans and C. gattii. Between the two species, C. neoformans var. grubii is the major causative agent of cryptococcosis in Asia. We investigated the molecular characteristics of 46 isolates of C. neoformans from patients with cryptococcosis between 2008 and 2012 in Seoul, Korea. All the isolates were determined to be C. neoformans var. grubii (serotype A), mating type $MAT{\alpha}$, and molecular type VNI by PCR-restriction fragment length polymorphism of the URA5 gene. Multilocus sequencing type (MLST) analysis using the International Society of Human and Animal Mycoses (ISHAM) consensus MLST scheme identified two sequence types (ST). Out of the 46 strains, 44 (95.7%) were identified as ST5, and remaining 2 were identified as ST31. Our study revealed that the clinical strains of C. neoformans in Korea are genetically homogeneous with the VNI/ST5 genotypes, and new appearance of VNI/ST31 genotype may serve as an important indicator of global genetic analysis.

The Characteristics of Housing Environment of the Elderly in Apartment (공동주택 거주 노인의 주거환경 특성에 관한 연구)

  • Jun, Eun-Jung
    • KIEAE Journal
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    • v.12 no.4
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    • pp.31-40
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    • 2012
  • This study of elderly households living conditions to determine the overall status in the housing environment is to understand. To this end, the stage of elderly as a framework to target living consciousness, living space composition, housing Environment composition of elderly households with a comprehensive understanding and analyzes were employed. For this research is a survey conducted on senior citizens(over 60) in Busan, Korea. The major findings are as followings; In the living consciousness, the elderly households showed high perception of dependency but the age of the elderly increases, depends on the parent to the child. Also depending on the aging of the elderly considered health more important than economic conditions. The requirements for living space composition, most respondents prefer to stay in the same place where they currently reside. Move into the new housing was also identified. It was recognized that elderly households not only required living in co-residential space with their families, but also the necessity for individual space. The home environments composition, health care and social welfare support services appears overall the elderly of stage. The age of the elderly increases, a strong demand for care and support service were identified. Health activities in leisure and social activities and social activities, as age increased mainly done by the convenience of public transportation has been identified that are important.