• 농업과학연구
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• 제38권1호
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• pp.51-63
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• 2011

BoLA (bovine leukocyte antigens) have been known as gene complex related with bovine diseases and immunological traits. This study was conducted to find out the characteristics of BoLA-DRB3 gene related to mastitis and BL(bovine leukocyte) from 280 cattle [193 animals of Holstein cattle and 87 animals of Hanwoo]. As a result, five PCR-RFLP types (b, d, e, f and g) using HaeIII restriction enzyme, three BstYI restriction patterns (b, d and e) and eight RsaI restriction types(b, d, f, I, j, n, o and w) were identified. Moreover, we identified new d' type ($197{\rightarrow}175$/22), having one more cutting site by BstYI enzyme than d type allele and n' type ($180{\rightarrow}169$/11) having one more cutting site by RsaI enzyme than n allele was additionally identified. Next, we identified 53 SNPs in BoLA-DRB3 exon2 from 280 cattle. SNP frequency and heterozygosity of Holstein and Hanwoo were investigated in all the SNP genotype. These results might be based on research for identifying marker associated with bovine diseases.

• 한국임상약학회지
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• 제25권2호
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• pp.120-129
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• 2015

Objectives: Drug utilization review program in Korea has provided 'drug combinations to avoid (DCA)' alerts to physicians and pharmacists to prevent potential adverse drug events or inappropriate drug use. Seven hundred and six DCA pairs have been announced officially by the Ministry of Food and Drug Safety (MFDS) by March, 2015. Some DCA pairs could be grouped based on the drug interaction mechanism and its consequences. This study aimed to investigate the drug-drug interaction (DDI) pairs, which may be potential DCAs, generated by the drug class-drug class interaction method. Methods: Eleven additive/synergistic and one antagonistic drug class-drug class interaction groups were identified. By combining drugs of two interacting drug class groups, numerous DDI pairs were made. The status and severity of DDI pairs were examined using Lexicomp and Micromedex. Also, the DCA listing rate was calculated. Results: Among 258 DDI pairs generated by the drug class-drug class interaction method, only 142 pairs were identified as official DCA pairs by the MFDS. One hundred and four pairs were identified as potential DCA pairs to be listed. QT prolonging agents-QT prolonging agents, triptans-ergot alkaloids, tricyclic antidepressants-monoamine oxidase inhibitors, and dopamine agonists-dopamine antagonists were identified as drug class-drug class interaction groups which have less than 50 % DCA listing rate. Conclusion: To improve the clinicians' adaptability to DCA alerts, the list of DCA pairs needs to be continuously updated.

• 디지털융복합연구
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• 제11권8호
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• pp.109-117
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• 2013

본 연구는 개인의 길찾기 능력이 방문자태도에 미치는 영향에 대해 알아보고자 하였다. 선행 문헌을 분석하여 길찾기와 길찾기 능력에 대해 살펴보았으며, 이러한 길찾기의 능력이 장소에 대한 방문자들의 태도와 어떠한 관계성이 있는 가를 확인하였다. 연구결과 첫째, 이용자들의 길찾기 능력은 이용만족도에 부분적으로 영향을 미치는 것으로 확인되었다. 둘째, 이용자들의 길찾기 능력은 재방문의도에 부분적으로 영향을 미치고 있는 것으로 확인되었다. 셋째, 이용자들의 길찾기 능력은 추천의도에 부분적으로 영향을 미치고 있는 것으로 확인되었다. 마지막으로, 이용자들의 이용만족도는 재방문의도 및 추천의도에 영향을 미치는 것으로 확인되었다. 이상의 본 연구결과는 지하상업시설물이 갖는 공간구조상의 특성을 감안한 지상과는 다른 형태의 커뮤니케이션 디자인이 필요하다는 것, 특히 복합상업시설물이 갖는 서비스 공간의 주요 사항을 고려한 마케팅 전략이 필요하다는 실무적 의의를 갖는다고 할 수 있다.

• 한국균학회소식:학술대회논문집
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• 한국균학회 2014년도 춘계학술대회 및 임시총회
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• pp.41-41
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• 2014

Pinewood nematode (PWN, Bursaphelenchus xylophilus) is a serious pathogenic worm that quickly dry pine trees to death. Recently, PWN has been devastating huge amounts of conifer trees in Korea. As a first step to explore the association and ecological roles of fungi in PWN life cycle in Korea, in this study we first isolated and indentified fungi from PWN-infested Korean pine and Japanese black pine wood sampled in Jinju, Sacheon, Pocheon, Chuncheon, Gwangju, and Hoengseong in Korea. A total of 144 fungal isolates were obtained from Japanese black pine wood and 264 fungal isolates from Korean pine wood. Their morphology and nucleotide sequences of the ITS rDNA and ♌-tubulin gene were examined for species identification. Ophiostoma ips, Botrytis anthophila, Penicillium sp., Hypocrea lixii, Trichoderma atroviride, O. galeiforme, Fusarium proliferatum were identified from Japanese black pine wood. Leptographium koreanum, L. pini-densiflorae, Ophiostoma ips, Penicillium raistrick, Trichoderma sp. were isolated from Korean pine wood. O. ips and L. koreanum were the major species on the two different PWN-infected pine tree. The cultivation of PWN on fungal mat of the identified species did some enhance PWN reproduction. The ambrosia beetle, Platypus koryoensis, is a serious pest of oak trees in Korea. In this study we investigated filamentous fungi present in the body of the beetle. Fourteen genera of filamentous fungi belonging to Ascomycota and Basidiomycota were isolated. All the obtained genera were isolated in the mitosporic state. The identified fungi were classified in 11 distinct orders including the Ascomycota (Eurotiales, Hypocreales, Microascales, Ophiostomatales, Pleosporales, and Sordiales) and Basidiomycota (Agaricales, Corticiales, Polyporales, and Russulales Xylariales). Within Ascomycota, 13 species were found. Meanwhile five species were found within Basidiomycota. The results showed the presence of diverse fungi in P. koryoensis. Among the isolated fungi, some were able to produce wood degrading enzymes. Further fungal isolation was performed with P. koryoensis infested Quercus mongolica trees sampled at Kumdan mountain in Hanam-Si, Gyeonggi province from June of 2009 to June of 2010. Penicillin spp. and Trichoderma spp. were the major species of mold fungi group. Pichia guilliermondii was the major species of mold yeast group. Raffaelea quercus-mongolicae was also isolated, but its isolation frequency was not high. Other species identified were Ambrosiella xylebori, Fusarium solani, Cryphonectria nitschke, Chaetomium globosum, and Gliocladium viride, Candida kashinagacola, C. maritima, C. vanderkliftii, Saccharomycopsis crataegensis.

• 한국환경복원기술학회지
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• 제20권3호
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• pp.33-44
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• 2017

This study investigated in the vascular plants of special protection zones (the native land of Berchemia berchemiaefolia in Sadam-ri and Gallon valley) of Songnisan National Park and identified the whole vascular plants. The numbers of vascular plants were summarized as 273 taxa including 82 families, 182 genera, 236 species, 3 subspecies, 28 varieties and 6 forms. Woody plants were identified as 124 taxa (45.4%) and herbaceous plants as 149 taxa (54.6%). A total of 107 taxa were identified in the area of Sadam-ri, and 246 taxa were found in the Gallon valley. A total of 8 taxa of rare plants were identified, each of which was divided into 1 taxa of endangered (EN; Daphne pseudomezereum var. koreana), 3 taxa of vulnerable (VU; Paeonia japonica, Berchemia berchemiaefolia and Iris minutiaurea), 3 taxa of least concern (LC; Aristolochia contorta, Syringa patula var. kamibayshii and Goodyera schlechtendaliana) and 1 taxa of data deficient (DD; Alangium platanifolium). The Korean endemic plants were 9 taxa (Clematis trichotoma, Thalictrum actaefolium var. brevistylum, Aster koraiensis, Cirsium setidens, etc.). In the specific plants by floristic region were 34 taxa, a degree I were 17 taxa (Chloranthus japonicus, Celastrus stephanotifolius, etc.), 9 taxa of a degree II (Euonymus pauciflorus, Acer triflorum, etc.), 4 taxa of a degree III (Aconitum longecassidatum, Angelica gigas, etc.), 2 taxa of a degree IV (Ulmus macrocarpa and Daphne pseudomezereum var. koreana), 2 taxa of a degree V (Berchemia berchemiaefolia and Alangium platanifolium). The naturalized plants were 5 taxa including Fallopia dumetorum, Trifolium repens, Ailanthus altissima, Oenothera biennis, Erigeron annuus. Naturalization rate (NR) was 1.8% of all 273 taxa of vascular plants and the invasive alien plants were not found. The purpose of this study is to identify the flora that grows in the special protection zones of Songnisan National Park and provide the basic data for the management of the special protection zone in the future.

• 한국과학교육학회지
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• 제37권1호
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• pp.125-133
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• 2017

본 연구는 2012년부터 2015년도 사이에 진행된 고등학생 STEAM 기반 학생 연구과제에 대해 STEAM 요소 분석을 실시하여 STEAM 요소의 융합 양상 및 몇 가지 의미 있는 사례들에 대한 고찰을 실시하였다. 이 연구를 위해 4년 동안 진행된 400개 STEAM 기반 학생 연구과제들이 연구대상으로 선정되었다. 연구 결과는 다음과 같다. 첫째, STEAM 기반 학생 연구과제들을 진행하면서 STEAM 요소들 중 과학(S) 요소와 수학(M) 요소가 가장 많이 활용되었으며, 예술(A) 요소가 상대적으로 가장 적게 활용되었음을 알 수 있었다. 둘째, STEAM 융합 유형들 중 STEM 유형이 가장 많이 나타난 것으로 확인되었다. 연도별로 봤을 때, 특별한 경향을 보이지는 않으나, 연도가 지날수록 학생들이 STEAM 요소들을 더욱 다양한 방식으로 활용하고 있음을 확인할 수 있었다. 이러한 결과들을 종합한 결과, 학생들의 창의성을 자극하고, STEAM 요소들을 다양하게 활용하도록 도울 수 있는 안내와 지도 방안의 마련이 필요하다.

• BMB Reports
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• 제51권8호
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• pp.412-417
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• 2018

Homotypic cell-in-cell (CIC) structures forming between cancer cells were proposed to promote tumor evolution via entosis, a nonapoptotic cell death process. However, the mechanisms underlying their formation remained poorly understood. We performed a microarray analysis to identify genes associated with homotypic CIC formation. Cancer cells differing in their ability to form homotypic CIC structures were selected for the study. Association analysis identified 73 probe sets for 62 candidate genes potentially involved in CIC formation. Among them, twenty-one genes were downregulated while 41 genes were upregulated. Pathway analysis identified a gene interaction network centered on IL-8, which was upregulated in high CIC cells. Remarkably, CIC formation was significantly inhibited by IL-8 knockdown and enhanced upon recombinant IL-8 treatment, which correlated with altered cell-cell adhesion and expression of adhesive molecules such as P-cadherin and ${\gamma}$-catenin. Together, our work identified IL-8 as a positive regulator of homotypic CIC formation via enhancing intercellular adhesion.

• Genomics & Informatics
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• 제17권4호
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• pp.42.1-42.6
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• 2019

Robust identification of genetic alterations is important for the diagnosis and subsequent treatment of tumors. Screening for genetic alterations using tumor tissue samples may lead to biased interpretations because of the heterogeneous nature of the tumor mass. Liquid biopsy has been suggested as an attractive tool for the non-invasive follow-up of cancer treatment outcomes. In this study, we aimed to verify whether the mutations identified in primary tumor tissue samples could be consistently detected in plasma cell-free DNA (cfDNA) by digital polymerase chain reaction (dPCR). We first examined the genetic alteration profiles of three colorectal cancer (CRC) tissue samples by targeted next-generation sequencing (NGS) and identified 11 non-silent amino acid changes across six cancer-related genes (APC, KRAS, TP53, TERT, ARIDIA, and BRCA1). All three samples had KRAS mutations (G12V, G12C, and G13D), which were well-known driver events. Therefore, we examined the KRAS mutations by dPCR. When we examined the three KRAS mutations by dPCR using tumor tissue samples, all of them were consistently detected and the variant allele frequencies (VAFs) of the mutations were almost identical between targeted NGS and dPCR. When we examined the KRAS mutations using the plasma cfDNA of the three CRC patients by dPCR, all three mutations were consistently identified. However, the VAFs were lower (range, 0.166% to 2.638%) than those obtained using the CRC tissue samples. In conclusion, we confirmed that the KRAS mutations identified from CRC tumor tissue samples were consistently detected in the plasma cfDNA of the three CRC patients by dPCR.

• Journal of Plant Biotechnology
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• 제35권4호
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• pp.265-274
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• 2008

Glutathione S-transferases (GSTs) are multifunctional proteins encoded by a large gene family divided into Phi, Tau, Theta, Zeta, Lambda and DHAR classes on the basis of sequence identity. The Phi(F) and Tau(U) classes are plant-specific and ubiquitous. Their roles have been defined as herbicide detoxification and responses to biotic and abiotic stresses. Fifty-two members of the GST super-family were identified in the Arabidopsis thaliana genome, 13 members of which belong to the Phi class of GSTs (AtGSTFs). Based on the sequence similarities of AtGSTFs, 11 BAC clones were identified from Brassica rapa. Seven unique sequences of ORFs designated the Phi class candidates of GST derived from B. rapa (BrGSTFs) were detected from these 11 BAC clones by blast search and sequence alignment. Some of BrGSTFs were present in the same BAC clones indicating that BrGSTFs could also be clustered as usual in plant. They were mapped on B. rapa linkage group 2, 3, 9 and 10 and their nucleotide and amino acid sequences were highly similar to those of AtGSTFs. In addition, in silico analysis of BrGSTFs using Korea Brassica Genome Project 24K oligochip and microarray database for cold, salt and drought stresses revealed 15 unigenes to be highly similar to AtGSTFs and six of these were identical to one of BrGSTFs identified in the BAC clones indicating their expression. The sequences of BrGSTFs and unigenes identified in this study will facilitate further studies to apply GST genes to medical and agriculture purposes.

• 한국보건간호학회지
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• 제11권2호
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• pp.121-130
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• 1997

The community health nursing process is essential in providing community health nursing service to the community. It helps to identify community health problems. to prioritize problems, to provide service. and to evaluate service results. However. it is very rare to find a study which applies the community health nursing process. This study intended to apply the community health nursing process to a urban community. The focus of the study was a community consisting of 533 families in a region of Seoul. The study process was as follows: 1) The data was collected by conducting interviews with community leaders and by collecting surveys from the people of the community. 2) The data was summarized and analyzed. 3) The community nursing diagnosis was identified. 4) The nursing diagnosis was prioritized. 5) The general and specific objectives for service were identified. 6) A specific nursing plan was set up. 7) A detailed evaluation plan was established. Four community nursing diagnoses were identified from the community. 1) The utilization rate of health center was found to be low due to lack of knowledge about the health center and low accessibility. 2) High trafic accident rate due to narrow roads. 3) High prevalence of chronic disease due to inappropriate health behavior. 4) High noise level and foul smell due to inappropriate waste management. Among the four community nursing diagnoses. 'High prevalence of chronic diseases was identified as a priority community nursing problem. The criteria for prioritizing community nursing problems were as follows: number of people involved, fragility of clients. severity of the problem. availability. of resources. concern of the people. readiness of nurses. relevance to the national policy. This study describes the general and specific objectives to solve the high prevalence of chronic health problems. nursing plans. and an evaluation plan.