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메주 발효 관련 Bacillus, Enterococcus, Staphylococcus 속 우점종 확인 (Identification of the Predominant Bacillus, Enterococcus, and Staphylococcus Species in Meju, a Spontaneously Fermented Soybean Product)

  • 장미현;정도원;이종훈
    • 한국미생물·생명공학회지
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    • 제47권3호
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    • pp.359-363
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    • 2019
  • 국내 5개 지역에서 수집한 12개 메주로부터 Bacillus, Enterococcus, Staphylococcus 속 bacteria를 선택배지를 이용하여 분리하고, 16S ribosomal RNA 유전자 및 gmk(guanylate kinase) 유전자 염기서열분석을 통해 동정하여, 이들 속의 우점종을 검토하였다. Bacillus 속과 Enterococcus 속은 모든 시료로부터 검출되었고, Bacillus 속은 11개 시료에서 총균수 대비 15% 이상 검출되어 메주 발효에서 가장 우점하는 bacteria로 확인되었다. Staphylococcus 속은 6개 시료에서만 검출되었다. Bacillus 속으로 동정된 151개 분리주는 B. velezensis, B. sonorensis 순으로 우점하였고, B. subtilis, B. licheniformis가 그 뒤를 이었다. Enterococcus 속으로 동정된 165개 분리주 중, 163균주가 E. faecium으로 확인되었다. Staphylococcus 속으로 동정된 82개 분리주에는 6종의 coagulase-negative Staphylococcus 존재가 확인되었고, S. xylosus가 우점종으로 확인되었다.

배양법을 이용한 누룩 발효 관련 Bacillus 속, Staphylococcus 속 세균 및 유산균의 우점종 확인 (Identification of the Predominant Species of Bacillus, Staphylococcus, and Lactic Acid Bacteria in Nuruk, a Korean Starter Culture)

  • 서세영;정도원;이종훈
    • 한국미생물·생명공학회지
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    • 제51권1호
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    • pp.93-98
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    • 2023
  • 우리나라 4개 지역에서 상업적으로 제조한 8개 누룩을 구입하여 Bacillus 속, Staphylococcus 속 및 유산균을 선택적으로 검출할 수 있는 배지를 이용하여 세균을 분리하고, 최근 확립된 분류기준에 따라 동정하여 세균의 그룹 별 다양성 및 우점종을 검토하였다. 모든 시료로부터 Bacillus 속이 검출되었지만, 3개 시료에서는 Staphylococcus 속 또는 유산균이 검출되지 않았다. 유산균이 가장 많이 검출된 1개 시료를 제외한 7개 시료에서 Bacillus 속이 가장 많이 계수되었고, Staphylococcus 속은 가장 적은 수로 검출되었다. Bacillus 속 6개 종의 존재가 확인되었고, B. subtilis, B. velezensis, B. licheniformis가 우점으로 검출되었다. Staphylococcus 속 9개 종의 존재가 확인되었고, coagulase 음성 S. pseudoxylosus와 S. saprophyticus의 우점을 확인했다. 유산균은 Enterococcus 속, Lactobacillus 및 근연속, Pediococcus 속, Weissella 속 10종의 존재가 확인되었고, P. pentosaceus가 우점종으로 확인되었다.

항공정비 품질 엔지니어의 핵심역량에 관한 연구 (A Study on the Core Competency of Aircraft Maintenance Quality Engineer)

  • 최병선;박종훈;이상천
    • 산업경영시스템학회지
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    • 제46권2호
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    • pp.47-56
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    • 2023
  • This study identified the core competencies of aircraft maintenance quality engineers and compared the importance and retention of core competencies. Through literature research, 21 core competencies were derived in three areas of management technology, elemenal technology and collaboration technology, and a survey was conducted on the importance and retention of core competencies for 42 aircraft maintenance quality engineers. As a result of the survey, the importance of all core competencies of aircraft maintenance quality engineers is 3.95/5 on average, and the retention of all core competencies is 3.99/5 on average. 'Risk Management', 'Creating Document', 'Honesty/Moral' were identified as the most important competencies in each area, and 'Quality Management', 'Language', 'Honesty/Moral' were identified as the most possessed competencies in each area. An IPA (Importance-Performance Analysis) was performed to analyze the details. Through IPA, 'Risk Management' and 'Safety Management' were evaluated as having a low degree of retention compared to a high level of importance. Therefore, they were identified as a core competencies that need to be improved first. In addition, the characteristics of each core competency and the recognition level in the field were also identified. This study will be helpful in defining the roles and functions of aircraft maintenance quality engineers to improve flight quality and prevent aviation accidents.

Outcomes of Surgical Repair for Truncus Arteriosus: A 30-Year Single-Center Experience

  • Yu Ri Lee;Dong-Hee Kim;Eun Seok Choi;Tae-Jin Yun;Chun Soo Park
    • Journal of Chest Surgery
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    • 제56권2호
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    • pp.75-86
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    • 2023
  • Background: We investigated the long-term outcomes of truncus arteriosus repair at a single institution with a 30-year study period. Methods: Patients who underwent repair of truncus arteriosus between 1993 and 2022 were reviewed retrospectively. Factors associated with early mortality, overall attrition, and reintervention were identified using appropriate statistical methods. Results: In total, 42 patients were enrolled in this study. The median age and weight at repair were 26 days and 3.5 kg, respectively. Thirty patients (71.4%) underwent 1-stage repair. There were 8 early deaths (19%). In the univariable analysis, undergoing surgery before 2011 was associated with early mortality (p=0.031). The overall survival rate at 10 years was 73.8%. In the multivariable analysis, significant truncal valve (TrV) dysfunction (p=0.010), longer cardiopulmonary bypass time (p=0.018), and the earlier era of surgery (p=0.004) were identified as risk factors for overall mortality. During follow-up, 47 reinterventions were required in 27 patients (64.3%). The freedom from all-cause reintervention rate at 10 years was 23.6%. In the multivariable analysis, associated arch obstruction (p<0.001) and significant TrV dysfunction (p=0.011) were identified as risk factors for all-cause reintervention. Arch obstruction (p=0.027) and a number of TrV cusps other than 3 (p=0.014) were identified as risk factors for right ventricle to pulmonary artery (RV-PA) reintervention, and significant TrV dysfunction was identified as a risk factor for TrV reintervention (p=0.002). Conclusion: Despite recent improvements in survival outcomes after repair of truncus arteriosus, RV-PA or TrV reinterventions were required in a significant number of patients during follow-up.

IDENTIFICATION CODE OF INTERSTELLAR CLOUDS WITHIN IRAF

  • Lee, Young-Ung;Jung, Jae-Hoon;Kim, Hyun-Goo
    • 천문학논총
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    • 제12권1호
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    • pp.185-196
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    • 1997
  • We present a code which identifies individual clouds in crowded region using IMFORT interface within Image Reduction and Analysis Facility (IRAF). We define a cloud as an object composed of all pixels in longitude, latitude, and velocity that are simply connected and that lie above some threshold temperature. The code searches the whole pixels of the data cube in efficient way to isolate individual clouds. Along with identification of clouds it is designed to estimate their mean values of longitudes, latitudes, and velocities. In addition, a function of generating individual images (or cube data) of identified clouds is added up. We also present identified individual clouds using a $^{12}CO$ survey data cube of Galactic Anticenter Region (Lee et al. 1997) as a test example. We used a threshold temperature of $5\sigma$ rms noise level of the data With a higher threshold temperature, we isolated subclouds of a huge cloud identified originally. As the most important parameter to identify clouds is the threshold value, its effect to the size and velocity dispersion is discussed rigorously.

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복식미학 연구를 위한 방법론 제안 - 복식 비평 모델을 중심으로 - (Methodological Approaches to Aesthetic Research on Dress - Focused on a Model for Dress Criticism -)

  • 이예영
    • 대한가정학회지
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    • 제44권11호
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    • pp.35-42
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    • 2006
  • A criticism model for dress was developed to offer a methodological insight into research on aesthetics of dress. Concepts from Edmund B. Feldman's art criticism model, James D. Carney's style-relative model of art criticism, and Sung Bok Kim's fashion criticism model were borrowed and integrated to create a criticism model for dress, comprising identification of styles, descriptive features, aesthetic value features, external interpretation, socio-cultural interpretation, and evaluation. Both inductive and deductive approaches for the identification of styles can be made in the process of dress criticism. In the former case, descriptive features and aesthetic features are sequentially identified to locate the styles of dress. In the latter case, styles are identified first, and descriptive features and aesthetic features are identified accordingly. Logical criticisms can be made based on the critics' background knowledge of the history of dress and art.

A technique for the identification of friction at tool/chip interface during machining

  • Arrazola, P.;Meslin, F.
    • 한국윤활학회:학술대회논문집
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    • 한국윤활학회 2002년도 proceedings of the second asia international conference on tribology
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    • pp.319-320
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    • 2002
  • Numerical simulation of chip formation during high speed machining requires knowing the friction at tool/chip interface. This parameter is hardly identified and generally the loadings (temperature, force) during the identification are not similar to those encountered during machining. Thus, Coulomb friction identified with pin-on-disc device is often used to conduct numerical simulation. The used of this technique cannot leads to good numerical results of chip formation compared to the experimental tests especially in the case of low uncut chip thickness. In this contribution, we propose a new method to evaluate the friction at tool/chip interface. In fact several Coulomb friction parameters are identified corresponding to several parts of the cutting tool. Experimental tests have been conducted allowed us to determinate both the level and the distribution of the Coulomb friction. Experimental results are also compared to the results of orthogonal cutting simulation. We show that this technique allows predicting accuracy results of chip formation.

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Yeast Diversity of Wild Flowers in Islands, Jejudo, Ulleungdo and Yokjido of Korea

  • Lee, Jong-Soo;Hyun, Se-Hee;Kim, Ha-Kun
    • 한국균학회소식:학술대회논문집
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    • 한국균학회 2014년도 춘계학술대회 및 임시총회
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    • pp.30-30
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    • 2014
  • We isolated various yeasts from wild flowers in main islands, Jejudo, Ulleungdo and Yokjido of Korea and their yeasts were identified by comparison of their PCR-amplified D1/D2 regions of 26S rDNA using the BLAST database. Thirty two yeast strains of fourteen species were isolated from wild flowers of Jejudo. Forty eight yeast strains of twenty two species were isolated and identified from wild flowers of Ulleungdo, Korea. Sixty yeast strains belonged to twenty five species were isolated identified from wild flowers of Yokjido in Tongyeong, Korea. Only Metschnikowia reukaufii was overlapped from the three different islands areas. Two species overlapped from Jejudo and Ulleungdo: Pichia guilliermondii, Metschnikowia reukaufii. Seven species were overlapped from Ulleungdo and Yokjido: Cryptococcus albidus, Cryptococcus laurentii, Metschnikowia reukafii, Pichia scolyti, Rhodotorula glutinis, Rhodotorula graminis and Rhodotorula mucilaginosa. Four species were overlapped from Jejudo and Yokjido: Candida sp. Cryptococcus aureus, Metschnikowia reukafii and Pseudozyma sp.

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용매의 분획에 따른 겨자 물추출물의 항균성 (Antimicrobial Activities in the Water Extract of Mustard Seed Fractionated by Solvents)

  • 서권일;김홍출;심기환
    • 한국식품저장유통학회지
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    • 제4권3호
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    • pp.295-300
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    • 1997
  • The water extract of mustard seed was fractionated by solvents with an increase of polarity, and antimicrobial activities of each extracts were examined, and volatile compounds of each extract were identified by GC-MS. When the water extract was fractionated with solvents, the antimicrobial activities were high in the order of chloroform, ethylacetate, hexane, butanol and aqueous layer. In chloroform fraction, 16 volatile compounds, including 2 isothiocyanates such as 3-isothiocyanato- 1-propene and 4-isothiocyanato- 1-butane, 1 nitrile and 4 acids were identified, their contents were higher than other fractions. Twelve, 10, 4 and 7 volatile compounds were identified in ethylaceate, hexane, butanol and water fractions, respectively. The volatile compounds were considerably less in the fractions of butanol and water than others.

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낮선상황의 애착유형분류에 관한 일 연구 (A Study on Classification of Attachment on the Strange Situation)

  • 박응임;박성연
    • 대한가정학회지
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    • 제32권3호
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    • pp.159-170
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    • 1994
  • A study on classification of attachment on the Strange Situation was conducted. 55 infants(27 boys and 28 girls) whose attachment to mothers were assessed in the Strange Situation when they were 14 to 20 moths old The analysis was made according to Ainsworth's classificatory system as well as Main & Solomon's. The results were summarized as follows: 43 infants were identified as secure attachment (Group B) 9 infants as insecure-avoidant(Group A) and 1 infant as insecure-resistant (Group C) There were 2 infants identified as insecure-disorganized/disoriented(Group D) In the sub-classification Group B infants were classified into B1(14 infants) B2(11 infants) and B3(18 infants) Group A infants were classified into 8 A1s and 1 A2 The Group C infant was identified as C1 and B4 were found. Finally because of the majority of infants as Group B, the association between sub-classifications and infan's sex and month was examined. the result indicated no signicant relations between them.

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