• Journal of Microbiology and Biotechnology
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• v.18 no.5
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• pp.983-989
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• 2008

Human insulin is a hormone well-known to regulate the blood glucose level. Recombinant preproinsulin, a precursor of authentic insulin, is typically produced in E. coli as an inactive inclusion body, the solubilization of which needs the addition of reducing agents such as $\beta$-mercaptoethanol. To make authentic insulin, recombinant preproinsulin is modified enzymatically by trypsin and carboxypeptidase B. The effects of $\beta$-mercaptoethanol on the formation of human insulin derivatives were investigated in the enzymatic modification by using commercially available human proinsulin as a substrate. Addition of 1 mM $\beta$-mercaptoethanol induced the formation of various insulin derivatives. Among them, the second major one, impurity 3, was found to be identical to the insulin B chain fragment from $Phe_1$ to $Glu_{21}$. Minimization of the formation of insulin derivatives and concomitant improvement of the production yield of human insulin were achieved by the addition of hydrogen peroxide. Hydrogen peroxide bound with $\beta$-mercaptoethanol and thereby reduced the negative effects of $\beta$-mercaptoethanol considerably. Elimination of the impurity 3 and other derivatives by the addition of over 10 mM hydrogen peroxide in the presence of $\beta$-mercaptoethanolled to a 1.3-fold increase in the recovery efficiency of insulin, compared with those for the case without hydrogen peroxide. The positive effects of hydrogen peroxide were also confirmed with recombinant human preproinsulin expressed in recombinant E. coli as an inclusion body.

• Journal of Microbiology and Biotechnology
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• v.23 no.1
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• pp.85-87
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• 2013

An outbreak of Staphylococcus aureus infections occurred in a university with an enrollment of 80 students in the city of Daejon, Republic of Korea. All nine S. aureus isolates from patients (n = 7), staff members (n = 1), and the fried chicken served as the lunch (n = 1) harbored the enterotoxin A gene and showed an identical antibioticresistant profile, PFGE banding pattern (STAS16.001), and sequence type, ST 6. These results suggested that the outbreak was associated with eating the fried chicken that had been handled by an infected staff member. This case report demonstrated a practical approach to identifying the source and transmission of an infection.

• Journal of Electrical Engineering and Technology
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• v.7 no.2
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• pp.178-183
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• 2012

The design of the integrated quench protection (QP) system for the high current superconducting magnet (SCM) has been fabricated and tested for the toroidal field (TF) coil system of the Korea Superconducting Tokamak Advanced Research (KSTAR) device. The QP system is capable of protecting the TF SCM, which consists of 16 identical coils serially connected with a stored energy of 495 MJ at the design operation level at 35.2 kA per turn. Given that the power supply for the TF coils can only ramp up and maintain the coil current, the design of the QP system includes two features. The first is a basic fast discharge function to protect the TF SCM by a dump resistor circuit with a 7 s time constant in case of coil quench event. The second is a slow discharge function with a time constant of 360 s for a daily TF discharge or for a stop demand from the tokamak control system. The QP system has been successfully tested up to 40 kA with a short circuit and up to 34 kA with TF SCM in the second campaign of KSTAR. This paper describes the characteristics of the TF QP systems and test results of the plasma experiment of KSTAR in 2009.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.8 no.5
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• pp.1026-1033
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• 2007

This paper compared simulation characteristics on the design of center strip by structure simulator with manufacture of 2.6GHz non-reciprocal element. Secures condition that affect in PIMD and confirmed change value of PIMD by change of condition. Implemented non-reciprocal element shows more than 20dB isolation characteristic at center frequency(2,650MHz) and has 0.2dB insertion loss in overall 100MHz operating bandwidth. Return losses of input and output port are measured below -20dB. IMD of non-reciprocal element exhibited superior value when there were no gaps between internal components and when materials having identical substances were used, and it showed an improving tendency when the pressure was increased higher, however, in case of applying pressure higher than a certain level, it results in damages, etc. of the magnet inside and ferrite as well, so then the characteristics of IMD was rapidly increased along with decrease of frequency characteristics.

• Journal of KIISE:Software and Applications
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• v.30 no.12
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• pp.1172-1184
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• 2003

At present, new techniques based on different component reference models for the integration of component and system of different platforms, such as EJB and COM+, are introduced. The operation between the components in the identical component platform is realized by the composition at the source level. In case of the different component platform, however, it is impossible to use combined components in real condition although they are components of similar domain. In this paper we proposed a solution for the composition problem by using component transformation methodology based on model between EJB and COM+ components which are different components. For the composition between EJB and COM+ components, we compared and analyzed each reference model, then proposed the Virtual Component Model which is implementation independent and the Implementation Table for the mutual conversion. Reffering to the Virtual Component Model and the Implementation Table, we can generalize each Implementation model to the Virtual Component Model, make the Virtual Component Model which is implementation independent through the virtual component modeling, transform EJB and COM+ components selectively. Proposing the effective Model Transformation method to the different component platform, we can combine EJB and COM+ components.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.15 no.2
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• pp.1089-1095
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• 2014

In this study, we compared and analyzed the displacement of final excavation with measured value through an inverse analysis method used in urban excavation construction. We maximized the effectiveness of the inverse analysis method, and plan to achieve cost-effective and practical construction management in the field with identical conditions. As the first stage, we suggest an example of a field which has the inverse analysis method. We applied the inverse analysis method to three different fields on which construction and measuring were finished. Of these three fields, two fields showed a very satisfactory result. However, in one field, there were significant differences between the analysis and measured value. The result of our analysis indicated that, we should unite the conditions of the inverse analysis method and field construction. We need to thoughtfully reconsider the RANKINE earth pressure application in a triangle type. This is because the uniformity of earth pressure is made by its arching effect, in the condition of the displacement of lower underground occurring widely, which is differentiated with the earth pressure conditions of RANKINE, even if the slurry wall has stiffness. Also, when recalculating the soil parameter, we should emphasize the adhesion of the weathering zone, and give experimental consideration to ground water level.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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• 2007.11a
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• pp.94-95
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• 2007

In this paper, the dependence of electrical characteristics of Silicon-$Al_2O_3$-Nitride-Oxide-Silicon (SANOS) memory cell transistors and program speed, reliability of memory device on interface trap between Si substrate and tunneling oxide was investigated. The devices were fabricated by the identical processing in a single lot except the deposition method of the charge trapping layer, nitride. In the case of P/E speed, it was shown that P/E speed is slower in the SONOS cell transistors with larger interface trap density by charge blocking effect, which is confirmed by simulation results. However, the data retention characteristics show much less dependence on interface trap. Therefore, to improve SANOS memory characteristic, it is very important to optimize the interface trap and charge trapping layer.

• Journal of Korean Society of Steel Construction
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• v.18 no.2
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• pp.203-211
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• 2006

This paper presents a new, effective torsional constant for thin-waled open beams under concentrated and uniformly distributed torques. The proposed constant can be used directly, instead of the St. Venant torsional constant, for any generic comemrcial finite-element program, without modifying the algorithm. The derived torsional constant accounts for both the pure torsion and the warping torsion, and is equal to the St. Venant torsion constant times a correction factor. It is also shown, in the case of the St. Venant torsion, that the derived constant is identical to the torsional constant. The derived effective torsional constant is different from the one given by Elhelbawey et al. The pure torsional shear stress, the warping shear stress, and the warping normal stress were also determine d, using the maximum twisting angle. The accuracy of the proposed torsional constant was validated by comparing the numerical results with the closed-form solutions or other numerical results available in the literature.

• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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• 2004.11a
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• pp.755-762
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• 2004

In our country, when the replacement for individual components of equipment in nuclear power plants is required, establishment of individual criteria i.e. Required Response Spectra(RRS) of seismic test/analysis for the component is very difficult because of the absence of Test Response Spectra(TRS) for the individual component to be replaced, from the existing qualification documents. In this case, it is required to perform the structural analysis for the nuclear equipment including the components to be replaced. After the structural analysis, Analysis Response Spectra(ARS) at the point of the component shall be generated and used for seismic test of the component. However, as of today, no standard program authorized for the response spectra generation by using the structural analysis exists in korea. Because of above reason, the STAR-Egs computer program was developed by using the method which calculates directly the expected response spectrum(frequency vs. acceleration type) of the selected points in the nuclear equipment with input spectrum(Required Response Spectra, RRS), based on the dynamic characteristics of the Finite Element(FE) model that is equivalent to the nuclear equipment. The STAR-Egs controls ANSYS/I-DEAS commercial software and automatically extract modal parameters of the FE model. The STAR-Egs calculates response spectrum using the established algorithm based on the extracted modal parameters, too. Reliance on the calculation result of the STAR-Egs was verified through comparison output with the result of MATLAB commercial software based on the identical algorithm. Moreover, actual seismic testing was performed as per IEEE344-1987 for the purpose of program verification by comparison of the FE analysis results.

• Cross-Cultural Studies
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• v.33
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• pp.309-330
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• 2013

This paper explores the concept of pure language introduced in Walter Benjamin's "The Task of the Translator" and looks at various perspectives on this concept represented in theories of Paul De Man and Gilles Deleuze and a short story of Jorge Luis Borges. According to "The Task of the Translator," pure language is defined as a vessel of which fragments are the original and the translation. Just as fragments are part of a vessel, so the original and the translation are fragments of a greater language, which is pure language. On the other hand, De Man, from a deconstructive criticism, says that pure language does not exist except as a permanent disjunction, which inhabits all languages as such, and that any work is totally fragmented in relation to this pure language and every translation is totally fragmented in relation to the original. While De Man consider pure language incorporeal, Deleuzian interpretation regards it as a virtual object or differenciator in relation to which the two series of the original and the translation coexist and resonate. Finally in Borges's "Pierre Menard, Author of the Quixote" Menard attempt to translate Cervantes's Don Quixote identically in every detail. By showing a case in which the original and the translation are the same, Borges raises a question what would take place in relation to pure language if the original and the translation were identical. In Deleuze, identity and resemblance are the result of a differenciator, but in Borges, identity is a differenciator which produces differences. If we apply this logic to the last paragraph of "The Task of the Translator," we can say the interlinear version of Scriptures, as the prototype or ideal of all translation, in the form of which the original and the translation must be one, is a differenciator, an endless difference-making machine.